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Article: Identification and validation of risk score model based on gene set activity as a diagnostic biomarker for endometriosis.

Zhang, Yi / Wu, Lulu / Wen, Xiang / Lv, Xiuwei

2023 Volume 9, Issue 7, Page(s) e18277

Abstract: Objective: The enigmatic nature of Endometriosis (EMS) pathogenesis necessitates investigating alterations in signaling pathway activity to enhance our comprehension of the disease's characteristics.: Methods: Three published gene expression profiles ...

Abstract	Objective: The enigmatic nature of Endometriosis (EMS) pathogenesis necessitates investigating alterations in signaling pathway activity to enhance our comprehension of the disease's characteristics. Methods: Three published gene expression profiles (GSE11691, GSE25628, and GSE7305 datasets) were downloaded, and the "combat" algorithm was employed for batch correction, gene expression difference analysis, and pathway enrichment difference analysis. The protein-protein interaction (PPI) network was constructed to identify core genes, and the relative enrichment degree of gene sets was evaluated. The Lasso regression model identified candidate gene sets with diagnostic value, and a risk scoring diagnostic model was constructed for further validation on the GSE86534 and GSE5108 datasets. CIBERSORT was used to assess the composition of immune cells in EMS, and the correlation between EMS diagnostic value gene sets and immune cells was evaluated. Results: A total of 568 differentially expressed genes were identified between eutopic and ectopic endometrium, with 10 core genes in the PPI network associated with cell cycle regulation. Inflammation-related pathways, including cytokine-receptor signaling and chemokine signaling pathways, were significantly more active in ectopic endometrium compared to eutopic endometrium. Diagnostic gene sets for EMS, such as homologous recombination, base excision repair, DNA replication, P53 signaling pathway, adherens junction, and SNARE interactions in vesicular transport, were identified. The risk score's area under the curve (AUC) was 0.854, as indicated by the receiver operating characteristic (ROC) curve, and the risk score's diagnostic value was validated by the validation cohort. Immune cell infiltration analysis revealed correlations between the risk score and Macrophages M2, Plasma cells, resting NK cells, activated NK cells, and regulatory T cells. Conclusion: The risk scoring diagnostic model, based on pathway activity, demonstrates high diagnostic value and offers novel insights and strategies for the clinical diagnosis and treatment of Endometriosis.
Language	English
Publishing date	2023-07-15
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2023.e18277
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Checkerboard corner detection method based on neighborhood linear fitting.

Du, Xin / Jiang, Benchi / Wu, Lulu / Xiao, Meng

Applied optics

2022 Volume 62, Issue 29, Page(s) 7736–7743

Abstract: To improve the calibration accuracy of a vision measurement system, a checkerboard corner detection method based on linear fitting of the checkerboard local contour is proposed. First, by binarization and morphological dilation of the checkerboard image, ...

Abstract	To improve the calibration accuracy of a vision measurement system, a checkerboard corner detection method based on linear fitting of the checkerboard local contour is proposed. First, by binarization and morphological dilation of the checkerboard image, the coordinates of two adjacent vertices of adjacent dark squares are obtained; the midpoint of the two vertices is taken as the reference point; the reference dotted array is obtained; and the Zernike moment subpixel method is used to obtain the checkerboard contour data points in the neighborhood of each reference point. Finally, the contour points are classified according to the orientation based on the reference points; two intersecting lines are fitted; and the intersection of the two lines is exactly the corner point that we want to find. A camera calibration experiment was conducted on the same group of checkerboard images. The results show that the calibration results of the corner points obtained based on this method are highly consistent with the OpenCV library function method and the MATLAB Toolbox calibration method, and the reprojection error is smaller. At the same time, it is robust to changes in the light source brightness.
Language	English
Publishing date	2022-10-28
Publishing country	United States
Document type	Journal Article
ISSN	1539-4522
ISSN (online)	1539-4522
DOI	10.1364/AO.497921
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Article ; Online: Multihydrophone Fusion Network for Modulation Recognition.

Wang, Haiwang / Wang, Bin / Wu, Lulu / Tang, Qiang

Sensors (Basel, Switzerland)

2022 Volume 22, Issue 9

Abstract: Deep learning (DL)-based modulation recognition methods of underwater acoustic communication signals are mostly applied to a single hydrophone reception scenario. In this paper, we propose a novel end-to-end multihydrophone fusion network (MHFNet) for ... ...

Abstract	Deep learning (DL)-based modulation recognition methods of underwater acoustic communication signals are mostly applied to a single hydrophone reception scenario. In this paper, we propose a novel end-to-end multihydrophone fusion network (MHFNet) for multisensory reception scenarios. MHFNet consists of a feature extraction module and a fusion module. The feature extraction module extracts the features of the signals received by the multiple hydrophones. Then, through the neural network, the fusion module fuses and classifies the features of the multiple signals. MHFNet takes full advantage of neural networks and multihydrophone reception to effectively fuse signal features for realizing improved modulation recognition performance. Experimental results on simulation and practical data show that MHFNet is superior to other fusion methods. The classification accuracy is improved by about 16%.
MeSH term(s)	Acoustics ; Communication ; Computer Simulation ; Neural Networks, Computer ; Recognition, Psychology
Language	English
Publishing date	2022-04-22
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s22093214
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Article ; Online: COVID-19 vaccination for patients with benign childhood epilepsy with centrotemporal spikes.

Yang, Xinyu / Wu, Lulu / Zheng, Dandan / Yang, Bin / Wu, De

Epilepsy & behavior : E&B

2022 Volume 134, Page(s) 108744

Abstract: Aim: There is a high demand for information on COVID-19 vaccination for patients with childhood epilepsy with centrotemporal spikes (BECTS). Patients with this condition need a stable, daily life; unfortunately, the decision of vaccination is not easy ... ...

Abstract	Aim: There is a high demand for information on COVID-19 vaccination for patients with childhood epilepsy with centrotemporal spikes (BECTS). Patients with this condition need a stable, daily life; unfortunately, the decision of vaccination is not easy for their parents. We evaluated patients with BECTS for symptoms and seizure control after COVID-19 vaccination. Methods: We asked the caregivers of all patients who visited our hospital to report their vaccination status, and if vaccinated, their experience in terms of adverse effects and seizure control after the second dose of the four Chinese-approved COVID-19 vaccines. Results: Seventy-seven children had received their second COVID-19 vaccine dose: 58 of 77 (75.3%) received Sinopharm (Beijing): BBIBP-CorV (Vero cells) and 16 (20.8%) received CanSino: Ad5-nCoV. Twenty of seventy-seven (25.97%) patients with BECTS reported having side effects; all effects were mild that could be relieved themselves. For Sinopharm (Beijing): BBIBP-CorV (Vero cells), the most frequent local side effect reported by the parents was pain at the site of injection (17.24%) and systematic side effect was fatigue (15.52%). For CanSino: Ad5-nCoV, the most reported local side effect was pain at the site of injection (6.25%). All parents reported that their child's side effects could be relieved by themselves. No patient reported status epilepticus or exacerbation of a pre-existing condition. If non-vaccinated, the cause of hesitation was explored: 40% of parents worried about inducing seizures, 19% of parents worried about vaccine side effects, 32% of parents worried about the vaccine-antiepileptic drug interactions, and 9% of parents feared for their child's physical condition. More than 34.1% of parents accepted that the decision to get the vaccine for their child was difficult. Over 90% of parents believe that research on the safety and tolerability of vaccination would help them to make the decision. Conclusion: Our data suggest that COVID-19 vaccination is well tolerated and safe in patients below 18 years of age having BECTS, thereby supporting the recommendation of vaccination.
MeSH term(s)	Animals ; COVID-19 ; COVID-19 Vaccines ; Child ; Chlorocebus aethiops ; Epilepsy, Rolandic ; Humans ; Pain ; Seizures ; Vaccination ; Vero Cells
Chemical Substances	COVID-19 Vaccines
Language	English
Publishing date	2022-05-17
Publishing country	United States
Document type	Journal Article
ZDB-ID	2010587-3
ISSN	1525-5069 ; 1525-5050
ISSN (online)	1525-5069
ISSN	1525-5050
DOI	10.1016/j.yebeh.2022.108744
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Article ; Online: Ocular emotion discrimination disorders in self-limited epilepsy patients with centrotemporal spikes complicated with electrical status epilepticus during sleep: A pediatric neuropsychological study.

Yang, Chunmei / Jiang, Nan / Wu, Lulu / Zhang, Kaili / Wang, Xiaocui / Yang, Bin

Brain and behavior

2023 Volume 13, Issue 7, Page(s) e3056

Abstract: Objective: We investigated the characteristics and factors influencing eye emotion recognition in self-limited epilepsy patients with centrotemporal spikes (SeLECTS) complicated with electrical status epilepticus during sleep (ESES).: Methods: We ... ...

Abstract	Objective: We investigated the characteristics and factors influencing eye emotion recognition in self-limited epilepsy patients with centrotemporal spikes (SeLECTS) complicated with electrical status epilepticus during sleep (ESES). Methods: We selected SeLECTS (n = 160) patients treated in the outpatient and inpatient departments of Anhui Children's Hospital from September 2020 to January 2022. According to the video electroencephalogram monitoring slow-wave index (SWI), SeLECTS patients with SWI < 50% were assigned into the typical SeLECTS group (n = 79), and patients with SWI ≥ 50% were assigned into the ESES group (n = 81). Patients in the two groups were assessed by The Eye Basic Emotion Discrimination Task (EBEDT) and The Eye Complex Emotion Discrimination Task (ECEDT), respectively. Comparisons were made with age-, sex- and education level-matched healthy control participants. The correlation between the characteristics of emotional discrimination disorder in the eye area and the clinical influencing factors was analyzed in ESES group, and p ≤ .050 was the threshold for significance. Results: Relative to the healthy control group, scores of sadness and fear in the typical SeLECTS group were markedly lower (p = .018, p = .023), while differences in scores of disgust, happiness, surprise, and anger were not significantly different between the groups (p = .072, p = .162, p = .395, p = .380, respectively). Compared with the healthy control group, the ESES group had significantly low scores in recognition of sadness, fear, disgust, and surprise (p = .006, p = .016, p = .043 and p = .038, respectively). However, differences in recognition of happiness and anger between the groups were not significant (p = .665 and p = .272). Univariate logistic analysis showed that the score of eye recognition for sadness in the ESES group was affected by age of onset, SWI, ESES duration and number of seizures. The score of eye recognition for fear was mainly affected by SWI, while the score of eye recognition for disgust was affected by SWI and number of seizures. The surprised eye emotion recognition score was mainly affected by the number of seizures. Variables with p < .1 were considered to be independent variables of multivariable ordered logistic regression. Multivariate logistic analysis showed that sadness emotion recognition was mainly affected by SWI and ESES duration, while disgust was mainly affected by SWI. Conclusion: The typical SeLECTS group showed impaired emotion (sadness and fear) recognition function in the eye area. The ESES group was associated with more intense emotional (sadness, fear, disgust, and surprise) recognition impairment in the eye region. The higher the SWI, the younger the onset age and the longer the duration of ESES, while the more the number of seizures, the more serious the impairment of emotional recognition function in the affected eye area.
MeSH term(s)	Child ; Humans ; Sleep ; Status Epilepticus/drug therapy ; Seizures/complications ; Epilepsy/complications ; Emotions ; Electroencephalography
Language	English
Publishing date	2023-05-16
Publishing country	United States
Document type	Journal Article
ZDB-ID	2623587-0
ISSN	2162-3279 ; 2162-3279
ISSN (online)	2162-3279
ISSN	2162-3279
DOI	10.1002/brb3.3056
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Article ; Online: The influence of placenta microbiota of normal term pregnant women on immune regulation during pregnancy.

Yang, Ping / Lu, Tong / Liang, Xinyuan / Huang, Ting / Wu, Lulu / He, Zonglin / Xiao, Xiaomin / Fan, Shangrong

BMC pregnancy and childbirth

2024 Volume 24, Issue 1, Page(s) 171

Abstract: Background: The concerted regulation of placenta microbiota and the immune responses secures the occurrence and development of pregnancy, while few studies reported this correlation. This study aimed to explore the relationship between the placenta ... ...

Abstract	Background: The concerted regulation of placenta microbiota and the immune responses secures the occurrence and development of pregnancy, while few studies reported this correlation. This study aimed to explore the relationship between the placenta microbiota and immune regulation during pregnancy. Methods: Twenty-six healthy pregnant women scheduled for elective cesarean section in the First Affiliated Hospital of Jinan University who met the inclusion criteria were recruited. Placenta and peripheral venous blood samples were collected. Microbiota in placental tissue was detected using high-throughput sequencing. Flow cytometry was used to detect immune cells in placental tissue and peripheral venous blood. ELISA and Luminex liquid chip technology were used to detect the content of cytokines in placental tissue and peripheral venous blood, respectively. Results: The placental microbiota has stimulating effects on the local immunity of the placenta and mainly stimulates the placental balance ratio CD56 + CD16 + /CD56 + CD16 and the placental macrophages, that is, it plays the role of immune protection and supporting nutrition. The stimulating effect of placental microbiota on maternal systemic immunity mainly induces peripheral Treg cells and B lymphocytes. Conclusion: The placental microbiota may be an important factor mediating local immune regulation in the placenta, and placental microbiota participates in the regulatory function of the maternal immune system.
MeSH term(s)	Pregnancy ; Female ; Humans ; Placenta ; Pregnant Women ; Cesarean Section ; Cytokines ; Microbiota
Chemical Substances	Cytokines
Language	English
Publishing date	2024-02-29
Publishing country	England
Document type	Journal Article
ZDB-ID	2059869-5
ISSN	1471-2393 ; 1471-2393
ISSN (online)	1471-2393
ISSN	1471-2393
DOI	10.1186/s12884-024-06353-x
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Article: Comprehensive effect of Naoxintong capsule combined with Western medicine on coronary heart disease after percutaneous coronary intervention: a meta-analysis.

Yu, Liyuan / Huang, Peiying / Wang, Meida / Li, Zhishang / Cai, Hairong / Feng, Yuchao / Wu, Lulu / Peng, Weihang / Zeng, Jing / Chen, Bojun

Frontiers in pharmacology

2024 Volume 15, Page(s) 1274000

Abstract: Aims: To systematically evaluate the comprehensive effect of combining Naoxintong capsule (NXT) with Western medicine (WM) on coronary heart disease post-percutaneous coronary intervention (PCI).: Methods: Randomized controlled trials (RCTs) of NXT ... ...

Abstract	Aims: To systematically evaluate the comprehensive effect of combining Naoxintong capsule (NXT) with Western medicine (WM) on coronary heart disease post-percutaneous coronary intervention (PCI). Methods: Randomized controlled trials (RCTs) of NXT for patients with CHD after PCI were systematically searched across multiple databases, including the Cochrane Library, PubMed, Embase, Chinese National Knowledge Infrastructure (CNKI), Chinese Science and Technology Journal Database (VIP), and Wan Fang, from inception until 31 January 2023. Study selection, data extraction, and quality assessment were performed by two independent reviewers. The quality of the included studies was evaluated using version 2 of the Cochrane risk-of-bias tool (RoB 2), and data analysis was performed using R4.2.2. Results: Fifteen RCTs conducted between 2011 and 2022 and involving 1,551 patients were identified, with 774 and 777 patients in the experimental and control groups respectively. It was found that the NXT and WM combination was superior to the WM therapy alone in terms of the effective clinical rate (odds ratio [OR] = 4.69, 95% confidence interval [CI] = 2.13-10.30), effective rate in electrocardiogram (OR = 6.92, 95% CI = 3.44-13.92), effective rate in angina (OR = 5.90, 95% CI = 3.04-11.46), left ventricular ejection fraction (mean difference [MD] = 4.94, 95% CI = 2.89-6.99), brain natriuretic peptide (MD = -294.00, 95% CI = -584.60 to -3.39), creatine kinase-MB (MD = -7.82, 95% CI = -13.26 to -2.37), major adverse cardiovascular events (OR = 0.24, 95% CI = 0.14-0.43), maximum platelet aggregation rate (MD = -8.33, 95% CI = -11.64 to -5.01), and Chinese medicine evidence score (OR = 9.79, 95% CI = 3.57-26.85). However, there was no significant difference in cardiac troponin I level reduction (MD = -0.13, 95% CI = 0.35-0.09) or the occurrence of adverse medicine events (OR = 0.92, 95% CI = 0.41-2.05). Meta-regression and subgroup analyses indicated that NXT capsule dosage, treatment duration, and patient baseline characteristics contributed to the heterogeneity. Conclusion: A combination of NXT and WM can improve clinical outcomes in patients undergoing PCI. However, further studies are needed to confirm the reliability and safety of this combined treatment approach. Systematic review registration: PROSPERO, https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=369174, Identifier CRD42022369174.
Language	English
Publishing date	2024-03-25
Publishing country	Switzerland
Document type	Systematic Review
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2024.1274000
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Article ; Online: Study on analgesic effect of Shentong Zhuyu Decoction in neuropathic pain rats by network pharmacology and RNA-Seq.

Wang, Chen / Lin, Jian / Xie, Huiling / Chen, Lukui / Chen, Peng / Wu, Lulu / Gong, Qian / Xia, Dongbin / Wang, Xilong

Journal of ethnopharmacology

2024 Volume 330, Page(s) 118189

Abstract: Ethnopharmacological relevance: Shentong Zhuyu Decoction (STZYD) is a traditional prescription for promoting the flow of Qi and Blood which is often used in the treatment of low back and leg pain clinicall with unclear mechanism. Neuropathic pain (NP) ... ...

Abstract	Ethnopharmacological relevance: Shentong Zhuyu Decoction (STZYD) is a traditional prescription for promoting the flow of Qi and Blood which is often used in the treatment of low back and leg pain clinicall with unclear mechanism. Neuropathic pain (NP) is caused by disease or injury affecting the somatosensory system. LncRNAs may play a key role in NP by regulating the expression of pain-related genes through binding mRNAs or miRNAs sponge mechanisms. Aim of the study: To investigate the effect and potential mechanism of STZYD on neuropathic pain. Methods: Chronic constriction injury (CCI) rats, a commonly used animal model, were used in this study. The target of STZYD in NP was analyzed by network pharmacology, and the analgesic effect of STZYD in different doses (H-STZYD, M-STZYD, L-STZYD) on CCI rats was evaluated by Mechanical withdrawal thresholds (MWT) and thermal withdrawal latency (TWL). Meanwhile, RNA-seq assay was used to detect the changed mRNAs and lncRNAs in CCI rats after STZYD intervention. GO analysis, KEGG pathway analysis, and IPA analysis were used to find key target genes and pathways, verified by qPCR and Western Blot. The regulatory effect of lncRNAs on target genes was predicted by co-expression analysis and ceRNA network construction. Results: We found that STZYD can improve hyperalgesia in CCI rats, and H-STZYD has the best analgesic effect. The results of network pharmacological analysis showed that STZYD could play an analgesic role in CCI rats through the MAPK/ERK/c-FOS pathway. By mRNA-seq and lncRNA-seq, we found that STZYD could regulate the expression of Cnr1, Cacng5, Gucy1a3, Kitlg, Npy2r, and Grm8, and inhibited the phosphorylation level of ERK in the spinal cord of CCI rats. A total of 27 lncRNAs were associated with the target genes and 30 lncRNAs, 83 miRNAs and 5 mRNAs participated in the ceRNA network. Conclusion: STZYD has the effect of improving hyperalgesia in CCI rats through the MAPK/ERK/c-FOS pathway, which is related to the regulation of lncRNAs to Cnr1 and other key targets.
Language	English
Publishing date	2024-04-12
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2024.118189
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Zs.A 1494: Show issues

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bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Impairment of eye emotion discrimination in benign childhood epilepsy with centrotemporal spikes: A neuropsychological study.

Wu, Lulu / Yang, Xinyu / Zhang, Kaili / Wang, Xiaocui / Yang, Bin

Brain and behavior

2021 Volume 11, Issue 6, Page(s) e02154

Abstract: Purpose: To explore the characteristics of the impairment of eye emotional recognition and related clinical factors in children with benign childhood epilepsy with centrotemporal spikes (BECT).: Methods: The Eye Basic Emotion Discrimination Task and ... ...

Abstract	Purpose: To explore the characteristics of the impairment of eye emotional recognition and related clinical factors in children with benign childhood epilepsy with centrotemporal spikes (BECT). Methods: The Eye Basic Emotion Discrimination Task and Eye Complex Emotion Discrimination Task were used to study emotion discrimination in 33 recently diagnosed BECT patients and 33 BECT patients after complete remission compared to respective age- and gender-matched comparison participants. Results: The scores for discrimination of sadness, fear, and disgust were significantly lower in the newly diagnosed BECT group than in the comparison group (p = .004, p = .019, and p = .044, respectively), while scores for recognizing happiness, anger, and surprise were not significantly different between the two groups (p = .248, p = .586, and p = .540, respectively). Our analysis revealed that the BECT onset age influences the scores for recognition of sadness, fear, and disgust (OR = 1.795, 95% CI: 1.097 to 2.936, p = .020; OR=1.846, 95% CI: 1.124 to 3.034, p = .016; OR = 1.851, 95% CI: 1.131-3.029, p = .014). After remission, the scores for discrimination of happiness, anger, sadness, fear, disgust, and surprise of the BECT group were not significantly different from the comparison group (p = .588, p = .765, p = .752, p = .984, p = .328, and p = .339, respectively). Conclusions: In our study, newly diagnosed BECT patients exhibited emotion discrimination dysfunction, mainly related to sadness, fear, and disgust, and this dysfunction was more severe the younger the age of onset was. However, after BECT remission, the ability to discriminate emotions returned to normal.
MeSH term(s)	Anger ; Child ; Emotions ; Epilepsy ; Facial Expression ; Humans ; Recognition, Psychology
Language	English
Publishing date	2021-05-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	2623587-0
ISSN	2162-3279 ; 2162-3279
ISSN (online)	2162-3279
ISSN	2162-3279
DOI	10.1002/brb3.2154
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Article ; Online: Cavity Enhanced Multi-Channels Gases Raman Spectrometer.

Yang, Dewang / Liu, Qingsheng / Guo, Jinjia / Wu, Lulu / Kong, Andong

Sensors (Basel, Switzerland)

2021 Volume 21, Issue 11

Abstract: Raman spectroscopy has the advantages of multi-component detection, with a simple device and wide concentration ranges, and it has been applied in environmental monitoring and gas logging. However, its low sensitivity has limited its further applications. ...

Abstract	Raman spectroscopy has the advantages of multi-component detection, with a simple device and wide concentration ranges, and it has been applied in environmental monitoring and gas logging. However, its low sensitivity has limited its further applications. In fact, the Raman signal is not weak, but the utilization efficiency of the Raman signal is low, and most of the signal is wasted. Given this, in this paper we report a cavity-enhanced multi-channel gas Raman spectrometer with an eight-sided cuvette. First, we simulated the Raman scattering intensity at angles from 30 degrees to 150 degrees. The simulation results showed that the signal intensity at an angle of 45° is 1.4 times that observed at 90°. Based on the simulation results, we designed a three-channel sample cell for higher sensitivity. The results of these experiments showed that the sensitivity could be increased by adding all signal together, and the limit of detection (LOD) for CO
Language	English
Publishing date	2021-05-31
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s21113803
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