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Article ; Online: Bioactivity and genome analysis of Bacillus amyloliquefaciens GL18 isolated from the rhizosphere of Kobresia myosuroides in an alpine meadow.

Chen, L / Xie, Y L / Wu, X H / Wu, L L / Yang, J / Gao, Y / Mi, Y / Yang, F

2024 Volume 117, Issue 1, Page(s) 16

Abstract: The unique eco-environment of the Qinghai-Tibet Plateau breeds abundant microbial resources. In this research, Bacillus amyloliquefaciens GL18, isolated from the rhizosphere of Kobresia myosuroides from an alpine meadow, and the antagonistic activity, ... ...

Abstract	The unique eco-environment of the Qinghai-Tibet Plateau breeds abundant microbial resources. In this research, Bacillus amyloliquefaciens GL18, isolated from the rhizosphere of Kobresia myosuroides from an alpine meadow, and the antagonistic activity, bacteriostatic hydrolase activity, and low temperature, salt, and drought resistance of it were determined and analysed. The seedlings of Avena sativa were root-irrigated using bacteria suspensions (cell concentration 1 × 10
MeSH term(s)	Bacillus amyloliquefaciens/genetics ; Rhizosphere ; Grassland ; Sodium Chloride ; Peptide Hydrolases ; Cyperaceae
Chemical Substances	Sodium Chloride (451W47IQ8X) ; Peptide Hydrolases (EC 3.4.-)
Language	English
Publishing date	2024-01-08
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	214861-4
ISSN	1572-9699 ; 0003-6072
ISSN (online)	1572-9699
ISSN	0003-6072
DOI	10.1007/s10482-023-01917-x
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Article: First Report of Alternaria Blight of Potato Caused by Alternaria tenuissima in China.

Zheng, H H / Wu, X H

Plant disease

2019 Volume 97, Issue 9, Page(s) 1246

Abstract: Potato (Solanum tuberosum L.) is grown worldwide as a major food crop. Potato early blight is an important disease caused by Alternaria solani (4). In 2011, diseased potato leaves with blight symptoms were collected from 21 sites (incidence averaged 60% ... ...

Abstract	Potato (Solanum tuberosum L.) is grown worldwide as a major food crop. Potato early blight is an important disease caused by Alternaria solani (4). In 2011, diseased potato leaves with blight symptoms were collected from 21 sites (incidence averaged 60% for about 2,000 ha of potato fields examined) in Gansu Province, northwest China. Small pieces of tissue taken from the margin between healthy and diseased tissues were surface-disinfected in 0.3% NaOCl for 2 min, rinsed with sterilized, distilled water, then placed on potato dextrose agar (PDA) at 25°C in the dark. Two of 24 Alternaria isolates from single-spore cultures were identified preliminarily as A. tenuissima, and the remaining isolates as A. solani or A. alternata, based on morphological traits. Colony appearance on potato carrot agar (PCA) was loosely cottony under a day/night cycle of 8 h fluorescent light/16 h dark at 22°C for 7 days (3). The isolates were characterized by formation of unbranched conidial chains up to 12 conidia in length, with one or two lateral branches forming occasionally. Conidia were typically ovoid to obclavate, and ranged from 20.4 to 42.4 × 7.7 to 13.2 μm. Transverse septa and longitudinal septa of conidia varied from 1 to 6 and 0 to 2, respectively. Short conidiophores arose singly and were 15.1 to 76.8 μm long by 2.4 to 6.2 μm wide. The internal transcribed spacer (ITS) region of rDNA and partial coding sequence of a histone gene were amplified from genomic DNA of the two A. tenuissima isolates using the ITS1/ITS4 and H3-1a/H3-1b primers (2), respectively. The ITS sequences of the two isolates (GenBank Accession Nos. JX495165 and JX495166) were 100% identical to those of A. tenuissima strains sdau 07-100 and BL08-3 (GQ871507 and AB470887), as well as to other Alternaria species, but the partial histone gene sequences (JX495167 and JX495168) were 99% identical to those of A. tenuissima isolates CR27, MA1, MA6, and CN-L-01 (AF404622, AF404633, AF404634, and EF371552, respectively) with less similarity to those of other Alternaria spp. Therefore, the isolates were identified as A. tenuissima based on morphological and molecular characteristics. Pathogenicity tests were conducted by inoculating detached leaves (30 per isolate) from 45-day-old plants of potato cv. Favorita with 20 μl drops (one drop per leaf) of a conidial suspension containing 10
Language	English
Publishing date	2019-02-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	754182-x
ISSN	0191-2917
ISSN	0191-2917
DOI	10.1094/PDIS-08-12-0763-PDN
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Article: First Report of Potato Stem Canker Caused by Binucleate Rhizoctonia AG-A in Jilin Province, China.

Yang, Y G / Wu, X H

Plant disease

2019 Volume 97, Issue 9, Page(s) 1246

Abstract: Potato (Solanum tuberosum L.) stem canker caused by Rhizoctonia solani occurs in potato-growing regions all over the world and can result in severe losses in crop yield and quality. In late July 2011, potato subterraneous stems with stem cankers composed ...

Abstract	Potato (Solanum tuberosum L.) stem canker caused by Rhizoctonia solani occurs in potato-growing regions all over the world and can result in severe losses in crop yield and quality. In late July 2011, potato subterraneous stems with stem cankers composed of brownish, sunken lesions were observed at 15% incidence in seven sites in Jilin Province, northeast China. Samples were collected, and stem pieces (each 5 mm long) taken from the margins of the healthy and diseased tissues were surface-disinfested with 0.5% NaOCl for 2 min, rinsed with sterilized water, dried, then placed on potato dextrose agar at 25°C in the dark. Three (designated JL-3, JL-5-1, and JL-6) of seven Rhizoctonia isolates that developed from single hyphal tip transfers were identified preliminarily as binucleate Rhizoctonia (BNR) isolates (teleomorph Ceratobasidium Rogers). The colonies were white or light gray with fluffy aerial hyphae and no sclerotia after 14 days in culture. Hyphal cells were binucleate when stained with 4'-6-diamidino-2-phenylindole. Average hyphal diameters (mean ± standard deviation) of isolates JL-3, JL-5-1, and JL-6 were 4.8 ± 0.5 μm (range 4.1 to 5.6 μm), 4.4 ± 0.4 μm (range 3.9 to 5.2 μm), and 4.5 ± 0.3 μm (range 4.0 to 5.0 μm), respectively. The internal transcribed spacer (ITS) region of ribosomal DNA was amplified from genomic DNA with primers ITS1 and ITS4 and sequenced. BLASTn analysis indicated that the resulting sequences (GenBank Accession Nos. JX885459, JX885460, and JX885461 for JL-3, JL-5-1, and JL-6, respectively) were 100% identical to that of a Ceratobasidium sp. AG-A isolate CHR08-10 (HQ270171). So the three isolates were identified as BNR AG-A based on morphological and molecular characteristics. To determine pathogenicity of the BNR isolates, potato seed tubers (cv. Favorita), each with 3- to 5-mm-long sprouts, were inoculated with wheat seeds (sterilized by autoclaving twice at 121°C for 1 h with a 24-h interval between autoclavings) colonized with each isolate (1). One sprouted potato tuber was planted in a plastic pot with a single colonized wheat seed placed 10 mm above the uppermost sprout tip in a sand/sawdust mixture (1:2 v/v). Plants were incubated in a glasshouse at 25 to 27°C, and assessed after 21 days. The test was performed on 20 plants/isolate and the experiment was repeated. The incidence of plants inoculated with JL-3, JL-5-1, and JL-6 that developed stem canker symptoms averaged 11.1, 23.5, and 28.6%, respectively, whereas all control plants inoculated with sterilized wheat seeds remained asymptomatic. Rhizoctonia spp. were not reisolated from the control plants, whereas BNR isolates were reisolated consistently from symptomatic stems of the inoculated plants, and the identity confirmed by morphological and molecular characteristics as described above, fulfilling Koch's postulates. BNR AG-A has been reported to be pathogenic on soybean (Glycine max), pea (Pisum sativum), snap bean (Phaseolus vulgaris), and pak choi (Brassica chinensis) in China (4). Isolates of R. solani AG-3 are most often associated with potato stem canker (2), although unidentified BNR isolates were reported to cause mild symptoms on potato sprouts in Finland (1), and small lesions on potato roots and stems in the United Kingdom (3). To our knowledge, this is the first report of BNR AG-A causing potato stem canker in Jilin Province, one of the main potato-producing areas of China. References: (1) M. J. Lehtonen et al. Plant Pathol. 57:141, 2008. (2) L. Tsror. J. Phytopatology 158:649, 2010. (3) J. W. Woodhall et al. New Dis. Rep. 23:31, 2011. (4) G. H. Yang et al. J. Phytopathology 153:333, 2005.
Language	English
Publishing date	2019-02-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	754182-x
ISSN	0191-2917
ISSN	0191-2917
DOI	10.1094/PDIS-10-12-0912-PDN
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Article: First Report of Sugar Beet Rhizoctonia Crown and Root Rot Caused by Rhizoctonia solani AG-2-2IIIB in Shanxi Province of China.

Zhao, C / Wu, X H

Plant disease

2019 Volume 98, Issue 3, Page(s) 419

Abstract: Sugar beet (Beta vulgaris L.) is grown worldwide as the second largest sugar crop. Sugar beet crown and root rot is an economically serious disease mainly caused by Rhizoctonia solani (teleomorph Thanatephorus cucumeris) AG 2-2 and AG 4 (1). In July 2010, ...

Abstract	Sugar beet (Beta vulgaris L.) is grown worldwide as the second largest sugar crop. Sugar beet crown and root rot is an economically serious disease mainly caused by Rhizoctonia solani (teleomorph Thanatephorus cucumeris) AG 2-2 and AG 4 (1). In July 2010, at the 25- to 27-leaf stage, symptoms typically associated with crown and root rot, including dark brown to black lesions at the base of the petioles or circular to oval dark lesions (up to 10.0 mm in diameter) at the taproot, were observed on 15% of sugar beet plants collected from three sites in Shanxi Province, northern China. Pieces of internal root tissues cut from the margins between symptomatic and healthy-appearing tissue were disinfected with 0.5% NaOCl for 2 min, rinsed three times with sterile water, then placed on water ager (WA) for incubation at 25°C in the dark. After 2 days, single hyphal tips of three Rhizoctonia-like isolates (designated SX-RSD1, SX-RSD2, and SX-RSD3) were transferred to potato dextrose ager (PDA). Colonies of all isolates were brown and developed dark brown sclerotia (0.5 to 1.0 mm diameter) on the media surface after 4 and 7 days, respectively. Mycelia were branched at right angles with septa near the branches and slight constrictions at the bases of the branches were present. Average hyphal diameters of the three isolates were 8.1, 7.3, and 7.6 μm, respectively. Hyphal cells were determined to be multinucleate (4 to 9 nuclei per cell) by staining with 4'-6-diamidino-2-phenylindole (DAPI) (2). Anastomosis groups were determined by pairing with reference strains (kindly provided by N. Kondo, Hokkaido University, Japan) (2), and all three isolates anastomosed with R. solani AG-2-2IIIB. All three isolates grew well on PDA at 35°C, which separates AG-2-2IIIB from AG-2-2 IV. The internal transcribed spacer (ITS) region of rDNA was amplified from genomic DNA of these isolates with primers ITS1 (5'-TCCGATGGTGAACCTGCGG-3')/ITS4 (5'-TCCTCCGCTTATTGATATGC-3'). Sequences (GenBank Accession Nos. KC413984, KC413985, and KC413986) were over 99% identical to those of 19 R. solani AG-2-2 IIIB isolates (e.g., FJ492146.3; strain F510). Therefore, based on the molecular characteristics and the anastomosis assay, these three isolates were identified as R. solani AG-2-2IIIB. To determine the pathogenicity of the isolates, wheat seeds were autoclaved twice for 60 min at 121°C on consecutive days and inoculated with each isolate (3). Subsequently, wheat seeds (three seeds per plant) were placed around 8-week-old sugar beet (cv. HI0305) plants at 2 cm intervals to each root and 10 mm deep in soil. Plants were grown at 25 to 27°C for 7 days in a glasshouse. All inoculated plants developed symptoms of root rot, whereas control plants inoculated with sterilized wheat seeds remained healthy. R. solani AG-2-2IIIB was consistently re-isolated from the symptomatic root tissue and was confirmed by both morphological and molecular characteristics described above, fulfilling Koch's postulates. To our knowledge, this is the first report of R. solani AG-2-2IIIB on sugar beet in Shanxi Province of China. R. solani AG2-2IIIB has been reported to be pathogenic on wheat in China (4), which is often grown in rotation with sugar beet. This rotation could increase the risk of soilborne infection to either crop by R. solani AG2-2IIIB. References: (1) R. M. Harveson et al. Compendium of Beet Diseases and Pests, American Phytopathological Society. St. Paul, MN. 2009. (2) W. C. Kronland and M. E. Stanghellini. Phytopathology. 78:820, 1988. (3) M. J. Lehtonen et al. Plant Pathol. 57:141, 2008. (4) D. Z. Yu et al., Hubei Agric. Sci. 3:39, 2000.
Language	English
Publishing date	2019-02-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	754182-x
ISSN	0191-2917
ISSN	0191-2917
DOI	10.1094/PDIS-12-12-1202-PDN
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Article: First Report of Sugar Beet Seedling Damping-Off Caused by Binucleate Rhizoctonia AG-A in China.

Wang, P P / Wu, X H

Plant disease

2019 Volume 96, Issue 11, Page(s) 1696

Abstract: Sugar beet (Beta vulgaris L.) is grown worldwide and produces one-third of the world's sugar supply. Sugar beet seedling Rhizoctonia damping-off is an important disease mainly caused by Rhizoctonia solani AG-2, AG-4, and AG-5 (2). In 2010, diseased sugar ...

Abstract	Sugar beet (Beta vulgaris L.) is grown worldwide and produces one-third of the world's sugar supply. Sugar beet seedling Rhizoctonia damping-off is an important disease mainly caused by Rhizoctonia solani AG-2, AG-4, and AG-5 (2). In 2010, diseased sugar beet seedlings with about 20% incidence affected by damping-off, which showed dark brown lesions on the stems just below the soil surface and portions of the roots, were collected from nurseries in three locations in Heilongjiang province, northeast China. Root fragments taken from the margins of healthy tissues and lesions on roots were surface disinfected with 0.5% sodium hypochlorite for 2 min, rinsed with sterile water, then placed on potato dextrose agar (PDA) and incubated at 25°C in the dark. Three (designed HLJ-RAA1, HLJ-RAB1, HLJ-RAB2) of nine Rhizoctonia isolates were obtained from diseased tissues and preliminarily identified as binucleate Rhizoctonia (BNR) anamorph (teleomorph Ceratobasidium Rogers) species-like. Fungal colonies were white with large amounts of floccose, aerial hyphae. Hyphal cells were determined to be binucleate when stained with 4'-6-diamidino-2-phenylindole (DAPI) (1). No sclerotia were produced after 14 days on PDA. Average hyphal diameter of the three isolates were 4.2, 4.3, and 4.8 μm, respectively. Further, the internal transcribed spacer (ITS) region of rDNA was amplified from the genomic DNA extracted from hyphae by bead beating in 2% CTAB solution using stainless steel beads with primers ITS1 and ITS4. The ITS sequences (GenBank Accession Nos. JX073668, JX073669, and JX073670) were over 99% identical to those of more than 50 Ceratobasidium sp. AG-A isolates (e.g., GenBank Accession No. JQ688054.1; strain HY-15). Therefore, based on morphological and molecular characteristics, these isolates were identified to be BNR AG-A. To determine the pathogenicity of the isolates, sugar beet (cv. HI0305) seedlings were inoculated with wheat seeds colonized with each of the isolated Rhizoctonia strains (one seed per seedling), and grew in pots under greenhouse conditions (3). After 3 weeks, some inoculated plants showed damping-off as observed in the nurseries, whereas noninoculated control plants (sterile wheat seeds only) remained healthy. Disease incidence from the trials averaged 53.3%, 70%, and 53.3% for the isolates HLJ-RAA1, HLJ-RAB1, and HLJ-RAB2, respectively. The three BNR cultures of the pathogens were consistently reisolated from symptomatic roots, and their identities confirmed by morphological and molecular characteristics as described above, fulfilling Koch's postulates. BNR AG-A was previously reported to be pathogenic to soybean, pea, snap bean, and pak choy in China (4). However, to our knowledge, this is the first report of BNR AG-A causing sugar beet seedling damping-off in China. Sugar beet is often grown in crop rotation with soya bean and such a rotation could increase the risk of soilborne infection to either crop by BNR AG-A. References: (1) W. C. Kronland and M. E. Stanghellini. Phytopathology 78:820, 1988. (2) E. O'Sullivan and J. A. Kavanagh. Plant Pathol. 40:128, 1991. (3) C. E. Windels and D. J. Nabben. Phytopathology 79:83, 1989. (4) G. H. Yang et al. J. Phytopathol. 153:333, 2005.
Language	English
Publishing date	2019-02-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	754182-x
ISSN	0191-2917
ISSN	0191-2917
DOI	10.1094/PDIS-05-12-0492-PDN
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Article: First Report of Potato Stem Canker Caused by Rhizoctonia solani AG4 HGII in Gansu Province, China.

Yang, Y G / Wu, X H

Plant disease

2019 Volume 97, Issue 6, Page(s) 840

Abstract: Black scurf and stem canker on potato (Solanum tuberosum L.), caused by Rhizoctonia solani, is an important disease throughout the world. Isolates of R. solani AG3 are the principal cause of these diseases on potato (2). In August 2011, at the tuber ... ...

Abstract	Black scurf and stem canker on potato (Solanum tuberosum L.), caused by Rhizoctonia solani, is an important disease throughout the world. Isolates of R. solani AG3 are the principal cause of these diseases on potato (2). In August 2011, at the tuber bulking growth stage, symptoms typically associated stem canker, including dark brown stem lesions, were observed on 20% of potato plants collected from 23 locations (about 2,000 ha) in Gansu Province, northwest China. Stem pieces (each 5 mm long) taken from the margins of the healthy and diseased tissues were surface-disinfected with 0.5% NaOCl for 2 min, rinsed with sterilized water, dried, then placed on potato dextrose agar (PDA) at 25°C in the dark. Twenty-nine fungal isolates taken from single hyphal tips were identified as R. solani based on morphological traits, including mycelium branched at right angles with a septum near the branch and a slight constriction at the branch base. Hyphal cells were determined to be multinucleate (4 to 10 nuclei/cell) when stained with 4'-6-diamidino-2-phenylindole (DAPI). Anastomosis groups were determined by pairing with reference strains (kindly provided by N. Kondo, Hokkaido University, Japan), and three isolates (designed GS-15, GS-24, and GS-25) anastomosed with isolates of R. solani AG4. The internal transcribed spacer (ITS) region of rDNA was amplified from genomic DNA of each of the three isolates with primers ITS1 and ITS4. The resulting sequences (GenBank Accession Nos. JX843818, JX843819, and JX843820) were 100% identical to those of >10 R. solani AG4 HGII isolates (e.g., HQ629873.1; isolate ND13). Therefore, based on the anastomosis assay and molecular characteristics, the three isolates were identified as R. solani AG4 HGII. To determine pathogenicity of the AG4 HGII isolates, potato seed tubers (cv. Favorita) with 3 to 5 mm long sprouts were inoculated with wheat seeds (sterilized by autoclaving twice at 121°C for 1 h with a 24 h interval between autoclavings) colonized with each isolate (1). One sprouted tuber was planted in a sterilized plastic pot (1 liter) with a single colonized wheat seed placed 10 mm above the uppermost sprout tip in a sand/sawdust mixture (1:2 v/v, with dry heat sterilization at 161°C for 4 h before use). Plants were incubated in a glasshouse maintained at 25 to 27°C. The test was performed on 20 plants for each isolate, and the experiment was repeated. After 3 weeks, control plants inoculated with sterilized wheat seeds remained asymptomatic, and no Rhizoctonia spp. were isolated from these plants, whereas all inoculated plants showed symptoms of stem canker. R. solani AG4 HGII was reisolated consistently from symptomatic stems, and the identity of the reisolates confirmed by the morphological and molecular characteristics mentioned above, fulfilling Koch's postulates. Potato stem canker caused by R. solani AG4 HGII was reported previously in the United States (3). To our knowledge, this is the first report of R. solani AG4 HGII causing stem canker on potato in Gansu Province, the main potato-producing area of China. R. solani AG4 HGII can cause sheath blight on corn in China (4), which is commonly grown in rotation with potato. This rotation could increase the risk of soilborne infection to either crop by R. solani AG4 HGII. References: (1) M. J. Lehtonen et al. Plant Pathol. 57:141, 2008. (2) L. Tsror. J. Phytopathol. 158:649, 2010. (3) J. W. Woodhall et al. Plant Dis. 96:1701, 2012. (4) X. Zhou et al. J. Shenyang Agric. Univ. 43:33, 2012.
Language	English
Publishing date	2019-02-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	754182-x
ISSN	0191-2917
ISSN	0191-2917
DOI	10.1094/PDIS-09-12-0896-PDN
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Article: [Spindle cell type follicular adenoma of thyroid: report of a case].

Wu, X H / Liu, Y / Han, L / Chen, J W

Zhonghua bing li xue za zhi = Chinese journal of pathology

2021 Volume 50, Issue 11, Page(s) 1283–1285

MeSH term(s)	Adenocarcinoma, Follicular/surgery ; Adenoma ; Humans ; Thyroid Neoplasms
Language	Chinese
Publishing date	2021-09-01
Publishing country	China
Document type	Case Reports ; Journal Article
ZDB-ID	784533-9
ISSN	0529-5807
ISSN	0529-5807
DOI	10.3760/cma.j.cn112151-20210302-00175
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Article: [Research progress of non-coding RNA in pulmonary hypertension].

Su, H / Chen, E G / Wu, X H / Ying, K J

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases

2021 Volume 44, Issue 2, Page(s) 136–142

MeSH term(s)	Humans ; Hypertension, Pulmonary/genetics ; RNA, Untranslated/genetics ; Research
Chemical Substances	RNA, Untranslated
Language	Chinese
Publishing date	2021-01-30
Publishing country	China
Document type	Journal Article ; Review
ZDB-ID	1027965-9
ISSN	1001-0939
ISSN	1001-0939
DOI	10.3760/cma.j.cn112147-20200330-00434
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Article ; Online: Study on the influence of metformin on castration-resistant prostate cancer PC-3 cell line biological behavior by its inhibition on PLCε gene-mediated Notch1/Hes and androgen receptor signaling pathway.

Yang, Y / Wu, X-H

European review for medical and pharmacological sciences

2017 Volume 21, Issue 8, Page(s) 1918–1923

Abstract: Objective: To study the regulation of metformin on the biological behaviors of the castration-resistant prostate cancer (CRPC) PC-3 cell such as proliferation, invasion, apoptosis through influencing Notch1/Hes and androgen receptor (AR) signaling ... ...

Abstract	Objective: To study the regulation of metformin on the biological behaviors of the castration-resistant prostate cancer (CRPC) PC-3 cell such as proliferation, invasion, apoptosis through influencing Notch1/Hes and androgen receptor (AR) signaling pathway activity by its inhibition on the expression of PLCε gene. Materials and methods: Human prostate cancer-3 (PC-3) cell line was divided into PC-3 cell line (group A), PC-3 cell line + metformin (10 mM) (group B), PC-3 cell line + metformin (20 mM) (group C), PLCε gene knockout cell line (group D), PLCε knockout cell line + metformin (10 mM)_ (group E) and PLCε knockout cell line + metformin (20 mM) (group F), which were respectively tested at 24 h, 48 h and 72 h, and five duplicate wells were set at each time point in each group. Western blot assay and RT-PCR assay were used to test the relative expressions of PLCε, Notch1, Hes, AR protein and mRNA; MTT assay was used to test the cell proliferation. Transwell chamber was used to test the invasion capability. The scratch test was used to test the migration capability and the flow cytometer was used to test cell apoptosis. Results: The relative expressions of PLCε, Notch1, Hes, AR protein and mRNA in Group A were increased gradually with time, but those values in group B and group C were decreased gradually with time and also significantly lower than those in group A (p <0.05) at each time point. The relative expressions of PLCε, Notch1, Hes, AR protein and mRNA in-group D, group E and group F were not changed at each time point (p>0.05). The proliferation, invasion and migration capabilities of the cells in group A, group D, group E and group F were gradually increased with time, but those in group B and group C were rapidly decreased with time and also significantly lower than those in group A, group D, group E and group F (p<0.05) at each time point. The apoptosis rates of group B and group C were increased gradually with time, and there was no other significant change in each group (p<0.05). Conclusions: Metformin can regulate the biological behaviors of CRPC PC-3 cell line such as proliferation, invasion, migration and apoptosis through influencing Notch1/Hes and AR signaling pathway activity by its inhibition on the expression of PLCε gene.
MeSH term(s)	Apoptosis/drug effects ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Humans ; Male ; Metformin/pharmacology ; Neoplasm Invasiveness ; Phosphoinositide Phospholipase C/genetics ; Phosphoinositide Phospholipase C/physiology ; Prostatic Neoplasms, Castration-Resistant/pathology ; Receptor, Notch1/physiology ; Receptors, Androgen/physiology ; Signal Transduction/drug effects
Chemical Substances	NOTCH1 protein, human ; Receptor, Notch1 ; Receptors, Androgen ; Metformin (9100L32L2N) ; Phosphoinositide Phospholipase C (EC 3.1.4.11) ; phospholipase C epsilon (EC 3.1.4.11)
Language	English
Publishing date	2017-04-26
Publishing country	Italy
Document type	Journal Article
ZDB-ID	605550-3
ISSN	2284-0729 ; 1128-3602 ; 0392-291X
ISSN (online)	2284-0729
ISSN	1128-3602 ; 0392-291X
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Article ; Online: Physiological Response of Avena sativa to Low-Temperature Stress is Promoted by Bacillus amyloliquefaciens GL18 and Its Functional Genes

Chen, L. / Xie, Y. L. / Wu, X. H. / Yang, X. / Wang, T. / Peng, W. X.

Russ J Plant Physiol. 2022 Dec., v. 69, no. 7 p.161-161

2022

Abstract: Low temperature affects plant growth and biomass accumulation in the Qinghai-Tibet Plateau. Bacillus is an important plant growth-promoting rhizobacteria that can promote plant growth directly or indirectly. In this study, bacteria suspensions (cell ... ...

Abstract	Low temperature affects plant growth and biomass accumulation in the Qinghai-Tibet Plateau. Bacillus is an important plant growth-promoting rhizobacteria that can promote plant growth directly or indirectly. In this study, bacteria suspensions (cell concentration 1 × 10⁷ cfu/mL) of Bacillus amyloliquefaciens strain GL18 were interacted with Avena sativa seedlings by the root irrigation method under 4°C low-temperature conditions. The biomass accumulation, as well as the physiological and biochemical activities of A. sativa, was detected over 14 days. The results showed that the plant height, root length, fresh and dry weight of A. sativa treated at 4°C with B. amyloliquefaciens by root irrigation increased by 39.28, 32.50, 114.89, and 87.17%, respectively, compared to untreated control. The levels of plant hormones such as abscisic acid, salicylic acid and jasmonic acid were significantly increased, and the activities of superoxide dismutase, peroxidase and catalase increased rapidly and then decreased slowly. The accumulation of hydrogen peroxide and malondialdehyde decreased, and the contents of proline and betaine increased significantly. The genome of GL18 strain was sequenced and its related functional genes were analyzed. The results showed that genome of GL18 strain contained gene clusters encoding proteins related to the synthesis of lipopeptide compounds such as surfactin, iturin, and fengycin, encoding genes involved in 3-indole acetic acid synthesis as well as genes related to the synthesis of plant growth-promoting substances such as siderophore and polyamines. This study provided experimental data and a theoretical basis for the manner in which Bacillus promotes the growth of A. sativa under low-temperature stress.
Keywords	Avena sativa ; Bacillus amyloliquefaciens ; abscisic acid ; betaine ; biomass production ; catalase ; cold stress ; genes ; hydrogen peroxide ; indole acetic acid ; irrigation systems ; iturin ; jasmonic acid ; malondialdehyde ; peroxidase ; physiological response ; plant growth ; plant growth-promoting rhizobacteria ; plant height ; polyamines ; proline ; salicylic acid ; siderophores ; superoxide dismutase ; surfactin ; temperature ; China
Language	English
Dates of publication	2022-12
Size	p. 161.
Publishing place	Pleiades Publishing
Document type	Article ; Online
ZDB-ID	1166808-8
ISSN	1608-3407 ; 1070-3292 ; 1021-4437
ISSN (online)	1608-3407
ISSN	1070-3292 ; 1021-4437
DOI	10.1134/S1021443722601586
Database	NAL-Catalogue (AGRICOLA)

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