LIVIVO - Search results -

Search results

Result 1 - 10 of total 16

Search options

Article ; Online: Lentivirus-mediated short hairpin RNA interference of CENPK inhibits growth of colorectal cancer cells with overexpression of Cullin 4A.

Li, Xian / Han, Yi-Ru / Xuefeng, Xuefeng / Ma, Yong-Xiang / Xing, Guo-Sheng / Yang, Zhi-Wen / Zhang, Zhen / Shi, Lin / Wu, Xin-Lin

World journal of gastroenterology

2022 Volume 28, Issue 37, Page(s) 5420–5443

Abstract: Background: Colorectal cancer (CRC) is one of the most common malignant tumors worldwide. The identification of novel diagnostic and prognostic biomarkers for CRC is a key research imperative. Immunohistochemical analysis has revealed high expression of ...

Abstract	Background: Colorectal cancer (CRC) is one of the most common malignant tumors worldwide. The identification of novel diagnostic and prognostic biomarkers for CRC is a key research imperative. Immunohistochemical analysis has revealed high expression of centromere protein K (CENPK) in CRC. However, the role of CENPK in the progression of CRC is not well characterized. Aim: To evaluate the effects of knockdown of CENPK and overexpression of Cullin 4A (CUL4A) in RKO and HCT116 cells. Methods: Human colon cancer samples were collected and tested using a human gene expression chip. We identified CENPK as a potential oncogene for CRC based on bioinformatics analysis. Results: We demonstrated overexpression of CENPK in human colon cancer samples. CENPK was an independent risk factor in patients with CRC. The downstream genes FBX32, CUL4A, and Yes-associated protein isoform 1 were examined to evaluate the regulatory action of CENPK in RKO cells. Significantly delayed xenograft tumor emergence, slower growth rate, and lower final tumor weight and volume were observed in the CENPK short hairpin RNA virus infected group compared with the CENPK negative control group. The CENPK gene interference inhibited the proliferation of RKO cells Conclusion: We indicated a potential role of CENPK in promoting tumor proliferation, and it may be a novel diagnostic and prognostic biomarker for CRC.
MeSH term(s)	Humans ; Lentivirus/genetics ; RNA, Small Interfering/genetics ; Cullin Proteins/genetics ; Cullin Proteins/metabolism ; Cell Proliferation/genetics ; Colorectal Neoplasms/pathology ; Cell Line, Tumor ; Colonic Neoplasms/pathology ; Gene Expression Regulation, Neoplastic ; RNA Interference ; Cell Movement ; DNA-Binding Proteins/genetics ; Nuclear Proteins/metabolism
Chemical Substances	RNA, Small Interfering ; Cullin Proteins ; CENPK protein, human ; DNA-Binding Proteins ; Nuclear Proteins ; CUL4A protein, human
Language	English
Publishing date	2022-10-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	2185929-2
ISSN	2219-2840 ; 1007-9327
ISSN (online)	2219-2840
ISSN	1007-9327
DOI	10.3748/wjg.v28.i37.5420
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 6039: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾

Article ; Online: Epithelial-mesenchymal transition and metastasis of colon cancer cells induced by the FAK pathway in cancer-associated fibroblasts.

Xuefeng, Xuefeng / Hou, Ming-Xing / Yang, Zhi-Wen / Agudamu, Agudamu / Wang, Feng / Su, Xiu-Lan / Li, Xian / Shi, Lin / Terigele, Terigele / Bao, Li-Li / Wu, Xin-Lin

The Journal of international medical research

2020 Volume 48, Issue 6, Page(s) 300060520931242

Abstract: Objective: The role and mechanism of tetrathiomolybdate (TM) in cancer-associated fibroblasts (CAFs) in colon cancer using three-dimensional (3D) culture were investigated, and the associations between the focal adhesion kinase (FAK) pathway and ... ...

Abstract	Objective: The role and mechanism of tetrathiomolybdate (TM) in cancer-associated fibroblasts (CAFs) in colon cancer using three-dimensional (3D) culture were investigated, and the associations between the focal adhesion kinase (FAK) pathway and epithelial-mesenchymal transition (EMT) in CAFs were explored. Methods: A 3D co-culture model of colon cancer LOVO cells with CAFs and normal fibroblasts (NFs) was established using Matrigel as a scaffold material. The differential expression of LOXL2 (lysyl oxidase-like 2) in the supernatant of CAFs and NFs was determined using ELISA, and expression levels of EMT-related proteins and FAK signaling pathway-related proteins were determined using western blot. Results: LOXL2 levels secreted by CAFs were higher compared with that secreted by NFs. In the CAF + LOVO group, compared with the LOVO group, E-cadherin expression decreased significantly, while N-cadherin and F-PAK expression increased significantly. TM results were opposite compared with the above results. Conclusions: CAFs stimulate EMT in human colon cancer LOVO cells by secreting LOXL2 to activate the FAK signaling pathway, thereby promoting tumor metastasis. TM inhibited the occurrence of EMT in the CAF-induced colon cancer LOVO cell line, thereby reducing the invasion and metastasis of colon cancer cells.
MeSH term(s)	Cancer-Associated Fibroblasts ; Cell Line, Tumor ; Cell Movement ; Colonic Neoplasms ; Epithelial-Mesenchymal Transition ; Fibroblasts ; Focal Adhesion Protein-Tyrosine Kinases ; Humans
Chemical Substances	Focal Adhesion Protein-Tyrosine Kinases (EC 2.7.10.2)
Language	English
Publishing date	2020-06-25
Publishing country	England
Document type	Journal Article
ZDB-ID	184023-x
ISSN	1473-2300 ; 0300-0605 ; 0142-2596
ISSN (online)	1473-2300
ISSN	0300-0605 ; 0142-2596
DOI	10.1177/0300060520931242
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 925: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: The Synergistic Antitumor Effect of Tanshinone IIA Plus Adriamycin on Human Hepatocellular Carcinoma Xenograft in BALB/C Nude Mice and Their Influences on Cytochrome P450 CYP3A4

Liu, Tao-Li / Zhang, Li-Na / Gu, Yue-Yu / Lin, Mei-Gui / Xie, Jun / Chen, Yu-Ling / Liu, Jia-Hui / Wu, Xin-Lin / Mo, Sui-Lin

Advances in medicine

2020 Volume 2020, Page(s) 6231751

Abstract: Objective: Hepatocellular carcinoma is one of the most common diseases that seriously threaten human life and health. In this study, we evaluated the inhibitory effect of tanshinone IIA (Tan IIA) combined with adriamycin (ADM) on human hepatocellular ... ...

Abstract	Objective: Hepatocellular carcinoma is one of the most common diseases that seriously threaten human life and health. In this study, we evaluated the inhibitory effect of tanshinone IIA (Tan IIA) combined with adriamycin (ADM) on human hepatocellular carcinoma and developed a platform to assess the function if Chinese herbal ingredients combined with chemotherapy drugs have synergistic antitumor effects Methods: Established animal model of human hepatocarcinoma HepG2 cell in nude mice. Mice were divided into model control group, Tan IIA group, ADM group, and Tan IIA + ADM group. The changes from general condition, weight, tumor volume, and inhibition rate were observed. The data were gathered from serum AST level and histopathological changes. The content and activity of cytochrome P450 were determined by spectrophotometric analysis. CYP3A4 protein expression was analyzed by western blotting. The binding model crystal structure of Tan IIA and ADM with pregnane X receptor (PXR) was evaluated by Discovery Studio 2.1. Results: A combination of Tan IIA with ADM could improve life quality by relieving ADM toxicity, decreasing tumor volume, declining serum AST level, and improving liner pathological section in tumor-bearing mice. The inhibitory rates of Tan IIA, ADM, and cotreatment were 32.77%, 60.96%, and 73.18%, respectively. The Tan IIA group significantly enhanced the content of cytochrome b5, P450, and erythromycin- Conclusions: Tan IIA in combination with ADM could improve the life quality in tumor-bearing mice and enhance the antitumor effect. The Tan IIA group increased the concentration of cytochrome P450 enzymes and activity. Combined Tan IIA with ADM could upregulate the CYP3A4 protein expression and make relevant interaction with protein PXR by virtual docking.
Language	English
Publishing date	2020-02-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	2776733-4
ISSN	2314-758X ; 2314-758X
ISSN (online)	2314-758X
ISSN	2314-758X
DOI	10.1155/2020/6231751
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Genetic polymorphisms of DNA repair pathways influence the response to chemotherapy and overall survival of gastric cancer.

Zhou, Jing / Liu, Zhi-yue / Li, Cun-bao / Gao, Shang / Ding, Li-hong / Wu, Xin-lin / Wang, Zhao-yang

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

2015 Volume 36, Issue 4, Page(s) 3017–3023

Abstract: We aimed to evaluate the clinical response to platinum-based chemotherapy and treatment outcome of gastric cancer patients in the present of ERCC1, ERCC2, NBN, RAD51, and XRCC3 gene polymorphisms. A number of 415 patients of gastric cancer that received ... ...

Abstract	We aimed to evaluate the clinical response to platinum-based chemotherapy and treatment outcome of gastric cancer patients in the present of ERCC1, ERCC2, NBN, RAD51, and XRCC3 gene polymorphisms. A number of 415 patients of gastric cancer that received platinum-based chemotherapy were enrolled in the present study. The presence of ERCC1 rs11615 and rs2298881, ERCC2 rs1799793 and rs13181, NBN rs1805794, rs709816, and RAD51 rs1801321 and XRCC3 rs1799794 were determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Conditional regression analysis identified that CC genotype of ERCC1 rs11615 and AA genotype of ERCC2 rs1799793 was associated with a better response to chemotherapy in gastric cancer patients, and the odds ratio (ORs)(95% confidence interval (CI)) were 2.70(1.33-5.70) and 3.12(1.52-6.84), respectively. By the Cox analysis, the CC genotype of ERCC1 rs11615, AA genotype of ERCC2 rs1799793, and CC genotype of NBN rs1805794 were significantly associated with a longer overall survival (OS) of gastric cancer. In conclusion, our results suggest that ERCC1 rs11615, ERCC2 rs1799793, and NBN rs1805794 polymorphisms in the DNA repair pathways may influence the response to chemotherapy and OS of gastric cancer.
MeSH term(s)	Adult ; Aged ; Cell Cycle Proteins/genetics ; DNA Repair/drug effects ; DNA-Binding Proteins/genetics ; Endonucleases/genetics ; Female ; Genotype ; Humans ; Male ; Middle Aged ; Nuclear Proteins/genetics ; Platinum/therapeutic use ; Polymorphism, Genetic ; Polymorphism, Single Nucleotide/genetics ; Prognosis ; Rad51 Recombinase/genetics ; Stomach Neoplasms/drug therapy ; Stomach Neoplasms/genetics ; Survival Analysis ; Xeroderma Pigmentosum Group D Protein/genetics
Chemical Substances	Cell Cycle Proteins ; DNA-Binding Proteins ; NBN protein, human ; Nuclear Proteins ; X-ray repair cross complementing protein 3 ; Platinum (49DFR088MY) ; RAD51 protein, human (EC 2.7.7.-) ; Rad51 Recombinase (EC 2.7.7.-) ; ERCC1 protein, human (EC 3.1.-) ; Endonucleases (EC 3.1.-) ; Xeroderma Pigmentosum Group D Protein (EC 3.6.4.12) ; ERCC2 protein, human (EC 5.99.-)
Language	English
Publishing date	2015-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	605825-5
ISSN	1423-0380 ; 0289-5447 ; 1010-4283
ISSN (online)	1423-0380
ISSN	0289-5447 ; 1010-4283
DOI	10.1007/s13277-014-2936-3
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1598: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: RRS1 silencing suppresses colorectal cancer cell proliferation and tumorigenesis by inhibiting G2/M progression and angiogenesis.

Wu, Xin-Lin / Yang, Zhi-Wen / He, Li / Dong, Pei-De / Hou, Ming-Xing / Meng, Xing-Kai / Zhao, Hai-Ping / Wang, Zhao-Yang / Wang, Feng / Baoluri / Wurenqimuge / Agudamu / Jia, Yong-Feng / Shi, Lin

Oncotarget

2017 Volume 8, Issue 47, Page(s) 82968–82980

Abstract: Colorectal cancer (CRC) is one of the most common malignancies worldwide. Ribosome biogenesis regulatory protein homolog (RRS1) is an essential factor involved in ribosome biogenesis, while its role in CRC remains largely unclear. Here, we found that ... ...

Abstract	Colorectal cancer (CRC) is one of the most common malignancies worldwide. Ribosome biogenesis regulatory protein homolog (RRS1) is an essential factor involved in ribosome biogenesis, while its role in CRC remains largely unclear. Here, we found that RRS1 expression was significantly higher in CRC tissues compared with adjacent normal tissues. RRS1 High expression also predicted poor overall survival of CRC patients. Knockdown of RRS1 induced the G2/M cell cycle arrest, apoptosis and suppressed the proliferation of RKO and HCT-116 CRC cells. Furthermore, angiogenesis was also reduced in CRC cells after RRS1 knockdown. In addition, suppression of RRS1 blunted the tumor formation of CRC cells in nude mice. At the molecular level, silencing of RRS1 decreased the expression of M-phase inducer phosphatase 3 (CDC25C), Cyclin-dependent kinase 1 (CDK1), antigen KI-67 (KI67) and increased the protein level of cyclin-dependent kinase inhibitor 1 (CDKN1A) and tumor suppressor p53 (p53). Taken together, our findings provide evidence that RRS1 may promote the development of colon cancer. Therefore, targeting RRS1 may be a promising therapeutic strategy for CRC patients.
Language	English
Publishing date	2017-10-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	2560162-3
ISSN	1949-2553 ; 1949-2553
ISSN (online)	1949-2553
ISSN	1949-2553
DOI	10.18632/oncotarget.20897
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: [Effect of human colon carcinoma-associated fibroblasts on biological behavior of colon carcinoma Lovo cells].

Wu, Xin-lin / Lin, Kai-jin / Shi, Lin / Su, Xiu-lan / Hou, Ming-xing / Dong, Pei-de

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery

2013 Volume 16, Issue 8, Page(s) 759–763

Abstract: Objective: To investigate the effects of human colon carcinoma-associated fibroblasts (CAFs) on proliferation, adhesion, migration and invasion of colon carcinoma Lovo cells.: Methods: The co-culture models among colon CAFs, NFs and Lovo cell were ... ...

Abstract	Objective: To investigate the effects of human colon carcinoma-associated fibroblasts (CAFs) on proliferation, adhesion, migration and invasion of colon carcinoma Lovo cells. Methods: The co-culture models among colon CAFs, NFs and Lovo cell were established by conditioned medium (CM) of human colon CAFs and colon normal fibroblasts (NFs). Lovo cells in the blank control group was treated with serum-free culture medium. The effects of human colon CAFs on proliferation, adhesion, migration and invasion of colon carcinoma Lovo cells were detected by cell proliferation assay, adhesion assay, migration assay and Transwell invasion assay. Results: After co-culture with colon CAFs, the absorbance (A) value of Lovo cells was (0.667±0.059) in 48 h and (0.709±0.030) in 72 h. The A value of Lovo cells adhesion to fibronectin was (0.588±0.067). The cell mobility rates were (35.2±8.7)% in 12 h and (64.6±7.1)% in 24 h. The number of invasive cell was (56.2±4.8). All the above parameters were increased compared with those in the blank control group and NFs group (all P<0.01). Conclusion: Human colon CAFs can promote the proliferation, adhesion, migration and invasion of colon carcinoma Lovo cells.
MeSH term(s)	Cell Adhesion ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Coculture Techniques ; Colonic Neoplasms/pathology ; Fibroblasts/cytology ; Humans
Language	Chinese
Publishing date	2013-08
Publishing country	China
Document type	English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1671-0274
ISSN	1671-0274
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: [Clinical observation on colquhounia root tablet in treating lipid metabolism disturbance secondary to nephrotic syndrome].

Wu, Xin-lin / Li, Jun-biao / Mo, Sui-lin

Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine

2002 Volume 22, Issue 1, Page(s) 30–32

Abstract: Objective: To study the effect of Colquhounia root tablet (CRT) in treating nephrotic syndrome with sequential lipid metabolism disorder (NS-LMD).: Methods: The 96 patients with NS-LMD were randomly divided into two groups, the 60 cases in the ... ...

Abstract	Objective: To study the effect of Colquhounia root tablet (CRT) in treating nephrotic syndrome with sequential lipid metabolism disorder (NS-LMD). Methods: The 96 patients with NS-LMD were randomly divided into two groups, the 60 cases in the treated group treated with CRT and the 36 cases in the control group treated with hormone or cytotoxic medicine. The curative effect and the related indexes were determined before and after treatment. Results: After six months treatment, the general effective rate in the treated group was 88. 33%, which was markedly higher than that in the control group (69.44%, P < 0.05). The levels of the treated group in ameliorating lipid metabolism disorder and renal dysfunction were also higher than those in the control group (P < 0.05). Conclusion: CRT could improve NS-LMD, improve renal function, eliminate urinary protein and increase plasma albumin. It is highly effective with low toxicity and safe.
MeSH term(s)	Adolescent ; Adult ; Aged ; Cholesterol/blood ; Drugs, Chinese Herbal/therapeutic use ; Female ; Humans ; Hyperlipidemias/blood ; Hyperlipidemias/drug therapy ; Hyperlipidemias/etiology ; Lipoproteins, HDL/blood ; Male ; Middle Aged ; Nephrotic Syndrome/blood ; Nephrotic Syndrome/complications ; Nephrotic Syndrome/drug therapy ; Phytotherapy ; Triglycerides/blood
Chemical Substances	Drugs, Chinese Herbal ; Lipoproteins, HDL ; Triglycerides ; Cholesterol (97C5T2UQ7J)
Language	Chinese
Publishing date	2002-01
Publishing country	China
Document type	Clinical Trial ; English Abstract ; Journal Article ; Randomized Controlled Trial
ZDB-ID	1195456-5
ISSN	1003-5370
ISSN	1003-5370
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 2863: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Association between the GSTP1 codon 105 polymorphism and gastric cancer risk: an updated meta-analysis.

Bao, Li-Dao / Niu, Jian-Xiang / Song, Hui / Wang, Yi / Ma, Rui-Lian / Ren, Xian-Hua / Wu, Xin-Lin

Asian Pacific journal of cancer prevention : APJCP

2012 Volume 13, Issue 8, Page(s) 3687–3693

Abstract: Objective: The current meta-analysis was performed to address a more accurate estimation of the association between glutathione S-transferase P1 (GSTP1) codon 105 polymorphism and risk of gastric cancer (GC), which has been widely reported with ... ...

Abstract	Objective: The current meta-analysis was performed to address a more accurate estimation of the association between glutathione S-transferase P1 (GSTP1) codon 105 polymorphism and risk of gastric cancer (GC), which has been widely reported with conflicting results. Methods: A comprehensive literature search was conducted to identify all the relevant studies. Fixed or random effect models were selected based on the heterogeneity test. Publication bias was estimated using Begg's funnel plots and Egger's regression test. Results: A total of 20 studies containing 2,821 GC cases and 6,240 controls were finally included in the analyses. Overall, no significant association between GSTP1 polymorphism and GC risk was observed in worldwide populations. However, subgroup analysis stratified by ethnicity showed that GSTP1 polymorphism was significantly associated with increased risk of GC in Asians (G vs. A, OR = 1.273, 95%CI=1.011-1.605; GG vs. AA, OR=2.103, 95%CI=1.197- 3.387; GG vs. AA+AG, OR =2.103, 95%CI=1.186-3.414). In contrast, no significant association was found in Caucasians in any genetic models, except for with AG vs. AA (OR=0.791, 95%CI=0.669-0.936). Furthermore, the GSTP1 polymorphism was found to be significantly associated with GC in patients with H. pylori infection and in those with a cardiac GC. Subgroup analysis stratified by Lauren's classification and smoking status showed no significant association with any genetic model. No studies were found to significantly influence the pooled effects in each genetic mode, and no potential publication bias was detected. Conclusions: This meta-analysis suggested that the GSTP1 polymorphism might be associated with increased risk of GC in Asians, while GSTP1 heterozygote genotype seemed to be associated with reduced risk of GC. Since potential confounders could not be ruled out completely, further studies are needed to confirm these results.
MeSH term(s)	Case-Control Studies ; Codon/genetics ; Genetic Predisposition to Disease ; Glutathione S-Transferase pi/genetics ; Humans ; Polymorphism, Single Nucleotide/genetics ; Risk Factors ; Stomach Neoplasms/etiology
Chemical Substances	Codon ; GSTP1 protein, human (EC 2.5.1.18) ; Glutathione S-Transferase pi (EC 2.5.1.18)
Language	English
Publishing date	2012-10-24
Publishing country	Thailand
Document type	Journal Article ; Meta-Analysis
ZDB-ID	2218955-5
ISSN	2476-762X ; 1513-7368
ISSN (online)	2476-762X
ISSN	1513-7368
DOI	10.7314/apjcp.2012.13.8.3687
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Metastasis-Associated in Colon Cancer-1 Associates With Poor Prognosis and Promotes Cell Invasion and Angiogenesis in Human Cervical Cancer.

Zhou, Xiang / Xu, Chang-Juan / Wang, Jun-Xian / Dai, Ting / Ye, Ya-Ping / Cui, Yan-Mei / Liao, Wen-Ting / Wu, Xin-Lin / Ou, Jian-Ping

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society

2015 Volume 25, Issue 8, Page(s) 1353–1363

Abstract: Objective: The aim of this study is to investigate the clinicopathologic significance and potential role of metastasis-associated in colon cancer-1 (MACC1) in the progression of cervical cancer.: Methods: MACC1 expression was examined in cervical ... ...

Abstract	Objective: The aim of this study is to investigate the clinicopathologic significance and potential role of metastasis-associated in colon cancer-1 (MACC1) in the progression of cervical cancer. Methods: MACC1 expression was examined in cervical cancer cell lines, 6 matched cervical cancer tissues, and adjacent noncancerous tissues using Western blotting and real-time reverse transcriptase polymerase chain reaction. MACC1 protein expression and localization were determined in 181 paraffin-embedded archived cervical cancer samples using immunohistochemistry. Statistical analyses were applied to evaluate the clinicopathologic significance. The effects of MACC1 on cell migration, invasion, and angiogenesis were examined using migration assay, wound healing assay, 3-dimensional morphogenesis assay, and chicken chorioallantoic membrane assay. Western blotting was performed to examine the impact of MACC1 on the Akt and nuclear factor κB signaling pathways. Results: Both protein and messenger RNA levels of MACC1 was up-regulated in cervical cancer cell lines and cervical cancer tissues, as compared with normal tissues. High MACC1 expression was detected in 96 (53%) of 181 of the cervical cancer tissues. In addition, high MACC1 expression correlated significantly with aggressiveness of cervical cancer, including International Federation of Gynecology and Obstetric stage (P = 0.001), pelvic lymph node metastasis (P = 0.004), recurrence (P = 0.037), and poor survival (P = 0.001). Moreover, enforced expression of MACC1 in cervical cancer cell lines significantly enhanced cell migration, invasion, and angiogenesis. Conversely, knockdown of MACC1 caused an inhibition of cell migration, invasion, and angiogenesis. Up-regulation of MACC1 increased, but knockdown of MACC1 decreased the expression of matrix metalloproteinase-2 and matrix metalloproteinase-9. Furthermore, enforced expression of MACC1 could enhance, but knockdown of MACC1 could reduce AKT and nuclear factor κB pathway activity. Conclusions: Our findings suggest that MACC1 protein, as a valuable marker of cervical cancer prognosis, plays an important role in the progression of human cervical cancer cells.
MeSH term(s)	Adenocarcinoma/blood supply ; Adenocarcinoma/genetics ; Adenocarcinoma/pathology ; Biomarkers, Tumor/physiology ; Blotting, Western ; Carcinoma, Squamous Cell/blood supply ; Carcinoma, Squamous Cell/genetics ; Carcinoma, Squamous Cell/pathology ; Cell Movement ; Cell Proliferation ; Chorioallantoic Membrane/metabolism ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Immunoenzyme Techniques ; Neoplasm Grading ; Neoplasm Invasiveness ; Neoplasm Staging ; Neovascularization, Pathologic ; Prognosis ; RNA, Messenger/genetics ; RNA, Small Interfering/genetics ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction ; Survival Rate ; Transcription Factors/physiology ; Tumor Cells, Cultured ; Uterine Cervical Neoplasms/blood supply ; Uterine Cervical Neoplasms/genetics ; Uterine Cervical Neoplasms/pathology
Chemical Substances	Biomarkers, Tumor ; MACC1 protein, human ; RNA, Messenger ; RNA, Small Interfering ; Transcription Factors
Language	English
Publishing date	2015-08-28
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1070385-8
ISSN	1525-1438 ; 1048-891X
ISSN (online)	1525-1438
ISSN	1048-891X
DOI	10.1097/IGC.0000000000000524
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 3198: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: [A clinical research of Naoxinqing tablet's effects on blood fat and viscosity].

Wu, Xin-lin / Wang, Ming-jun / Kuang, Yu / Zhang, Wei-jun / Ao, Qin-xing

Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials

2008 Volume 31, Issue 1, Page(s) 171–174

Abstract: Objective: To investigate the effects on blood fat and viscosity of Naoxinqing tablet.: Methods: 66 patients of primary hyperlipoidemia were randomly divided into three groups. The first group was treated by Naoxinqing tablet, the second group was ... ...

Abstract	Objective: To investigate the effects on blood fat and viscosity of Naoxinqing tablet. Methods: 66 patients of primary hyperlipoidemia were randomly divided into three groups. The first group was treated by Naoxinqing tablet, the second group was treated by Naoxinqing and combined with half dose Simvastatin, the third group was treated by full dose Simvastatin. Then the clinical effect, fasting plasma lipid, the index of blood viscosity and side effect of the three group' patients were compared after 8 weeks. Results: The index of blood fat improved obviously after treatment of the three groups ( P < 0.05, P < 0. 01), group 2 and 3 were significantly superior to group 1 (P < 0.05), and no significance between group 2 and 3 (P > 0.05). Conclusions: The Naoxinqing tablet can improve the metabolism of blood fat, decrease blood viscosity obviously and treat hyperlipoidemia effectively with little side effect.
MeSH term(s)	Adult ; Aged ; Blood Viscosity/drug effects ; Cholesterol/blood ; Diospyros/chemistry ; Drugs, Chinese Herbal/administration & dosage ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Female ; Humans ; Hyperlipidemias/blood ; Hyperlipidemias/drug therapy ; Hypolipidemic Agents/administration & dosage ; Hypolipidemic Agents/pharmacology ; Hypolipidemic Agents/therapeutic use ; Lipids/blood ; Middle Aged ; Phytotherapy ; Plant Leaves/chemistry ; Plants, Medicinal/chemistry ; Simvastatin/administration & dosage ; Simvastatin/pharmacology ; Simvastatin/therapeutic use ; Tablets ; Treatment Outcome ; Triglycerides/blood
Chemical Substances	Drugs, Chinese Herbal ; Hypolipidemic Agents ; Lipids ; Tablets ; Triglycerides ; Cholesterol (97C5T2UQ7J) ; Simvastatin (AGG2FN16EV)
Language	Chinese
Publishing date	2008-01
Publishing country	China
Document type	English Abstract ; Journal Article ; Randomized Controlled Trial
ISSN	1001-4454
ISSN	1001-4454
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED