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Article: Combinatorial discovery of antibacterials

Wang, Cong / Wu, Yuhui / Xue, Yunfan / Zou, Lingyun / Huang, Yue / Zhang, Peng / Ji, Jian

2024 Volume 15, Issue 16, Page(s) 6044–6052

Abstract: The discovery of new antibacterials within the vast chemical space is crucial in combating drug-resistant bacteria such as methicillin- ... ...

Abstract	The discovery of new antibacterials within the vast chemical space is crucial in combating drug-resistant bacteria such as methicillin-resistant
Language	English
Publishing date	2024-03-26
Publishing country	England
Document type	Journal Article
ZDB-ID	2559110-1
ISSN	2041-6539 ; 2041-6520
ISSN (online)	2041-6539
ISSN	2041-6520
DOI	10.1039/d3sc06441g
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Article ; Online: Correlation between Physical Activity and Psychological Distress in Patients Receiving Hemodialysis with Comorbidities: A Cross-Sectional Study.

Wu, Yu-Hui / Hsu, Yu-Juei / Tzeng, Wen-Chii

International journal of environmental research and public health

2022 Volume 19, Issue 7

Abstract: Comorbidities cause psychological distress to patients on hemodialysis and cause their physical function to deteriorate. This study aims to examine whether physical patterns are associated with anxiety, depression and fatigue among patients with and ... ...

Abstract	Comorbidities cause psychological distress to patients on hemodialysis and cause their physical function to deteriorate. This study aims to examine whether physical patterns are associated with anxiety, depression and fatigue among patients with and without comorbidities who are on hemodialysis. To this end, a cross-sectional survey was administered to 120 patients on hemodialysis. Data were collected using the International Physical Activity Questionnaire—Short Form, Beck Depression Inventory—Second Edition, Beck Anxiety Inventory, and Brief Fatigue Inventory—Taiwan Version. An independent sample t test and generalized linear model analyses were conducted. The results revealed that patients with comorbidities exhibited more severe levels of depression (p < 0.001), anxiety (p < 0.001), and fatigue (p = 0.010) than patients without comorbidities. Additionally, patients on hemodialysis with a high physical activity level (≥600 metabolic equivalent of task per min/week) exhibited less depression (B = −4.03; p < 0.001; 95% confidence interval [CI] = −6.04, −2.03) and anxiety (B = −2.64; p = 0.002; 95% CI = −4.27, −1.00) severity than those with a low physical activity level; those who engaged in weekly physical activities exhibited less fatigue severity (B = −1.17; p = 0.001; 95% CI = −1.84, −0.49) and fatigue interference (B = −0.61; p = 0.015; 95% CI = −1.10, −0.12). For patients on hemodialysis, having comorbidities was correlated with more severe levels of depression, anxiety, and fatigue. Weekly moderate-intensity physical activities were revealed to be correlated with less severity levels of depression, anxiety, and fatigue. The study findings aid the development of interventions for promoting physical activity among patients on hemodialysis to prevent the exacerbation of complications caused by comorbidities and psychological distress.
MeSH term(s)	Anxiety/psychology ; Cross-Sectional Studies ; Depression/psychology ; Exercise ; Fatigue/epidemiology ; Fatigue/etiology ; Humans ; Psychological Distress ; Quality of Life ; Renal Dialysis/adverse effects
Language	English
Publishing date	2022-03-26
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2175195-X
ISSN	1660-4601 ; 1661-7827
ISSN (online)	1660-4601
ISSN	1661-7827
DOI	10.3390/ijerph19073972
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Article ; Online: Physical Activity and Health-Related Quality of Life of Patients on Hemodialysis with Comorbidities: A Cross-Sectional Study.

Wu, Yu-Hui / Hsu, Yu-Juei / Tzeng, Wen-Chii

International journal of environmental research and public health

2022 Volume 19, Issue 2

Abstract: Patients on hemodialysis with multiple comorbidities have limited physical activity, resulting in poor health, low activity participation, and low quality of life. Accordingly, the nursing care provided to such patients should include regular physical ... ...

Abstract	Patients on hemodialysis with multiple comorbidities have limited physical activity, resulting in poor health, low activity participation, and low quality of life. Accordingly, the nursing care provided to such patients should include regular physical activity training programs. Therefore, this cross-sectional descriptive study investigated whether patients on hemodialysis with and without comorbidities have different levels of physical activity and health-related quality of life (HRQoL); the correlations among the comorbidities, physical activity, and HRQoL of the two cohorts were also assessed. The 36-Item Short-Form Health Survey version 2 and International Physical Activity Questionnaire were employed to collect data from 120 patients on hemodialysis. An independent samples
MeSH term(s)	Cross-Sectional Studies ; Exercise ; Health Surveys ; Humans ; Quality of Life ; Renal Dialysis/methods
Language	English
Publishing date	2022-01-12
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2175195-X
ISSN	1660-4601 ; 1661-7827
ISSN (online)	1660-4601
ISSN	1661-7827
DOI	10.3390/ijerph19020811
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Article: [Effect of Curcumin on Apoptosis of Acute T-Lymphoblastic Leukemia Cells induced by UMI-77 and Its Related Mechanism].

Xu, Zheng / Song, Ling / Wu, Yu-Hui / Cao, Bo

Zhongguo shi yan xue ye xue za zhi

2022 Volume 30, Issue 3, Page(s) 695–703

Abstract: AbstractObjective: To explore the effect and mechanism of curcumin on human T-cell acute lymphoblastic leukemia (T-ALL) cell apoptosis induced by Mcl-1 small molecule inhibitors UMI-77.: Methods: T-ALL cell line Molt-4 was cultured, and the cells were ...

Abstract	AbstractObjective: To explore the effect and mechanism of curcumin on human T-cell acute lymphoblastic leukemia (T-ALL) cell apoptosis induced by Mcl-1 small molecule inhibitors UMI-77. Methods: T-ALL cell line Molt-4 was cultured, and the cells were treated with different concentrations of curcumin and Mcl-1 small molecule inhibitor UMI-77 for 24 h. The MTT method was used to detect the cell survival rate after different treatment; According to the results of curcumin and UMI-77, the experimental settings were divided into control group, curcumin group (20 μmol/L curcumin treated cells), UMI-77 group (15 μmol/L Mcl-1 small molecule inhibitor UMI-77 treated cells) and curcumin+ UMI-77 group (20 μmol/L curcumin and 15 μmol/L Mcl-1 small molecule inhibitor UMI-77 treated cells), MTT method was used to detect cell proliferation inhibition rate, Annexin V-FITC/PI double staining method and TUNEL staining were used to detect cell apoptosis, DCFH-DA probe was used to detect cell reactive oxygen species, JC-1 fluorescent probe was used to detect mitochondrial membrane potential, Western blot was used to detect the expression levels of apoptosis-related proteins and Notch1 signaling pathway-related proteins. Results: After the treatment of Molt-4 cells with different concentrations of curcumin and Mcl-1 small molecule inhibitor UMI-77, the cell survival rate was decreased (P<0.05); Compared with the control group, the cell proliferation inhibition rate of the curcumin group and the UMI-77 group were increased, the apoptosis rate of cell was increased, the level of ROS was increased, the protein expression of Bax, Caspase-3 and Caspase-9 in the cells were all increased, and the protein expression of Bcl-2 was reduced (P<0.05); Compared with the curcumin group or UMI-77 group, the cell proliferation inhibition rate and apoptosis rate of the curcumin+UMI-77 group were further increased, and the level of ROS was increased. At the same time, the protein expression of Bax, Caspase-3 and Caspase-9 in the cells were all increased, the protein expression of Bcl-2 was reduced (P<0.05); In addition, the mitochondrial membrane potential of the cells after curcumin treatment was decreased, and the proteins expression of Notch1 and HES1 were reduced (P<0.05). Conclusion: Curcumin can enhance the apoptosis of T-ALL cells induced by Mcl-1 small molecule inhibitor UMI-77 by reducing the mitochondrial membrane potential, the mechanism may be related to the inhibition of Notch1 signaling pathway.
MeSH term(s)	Apoptosis ; Apoptosis Regulatory Proteins ; Caspase 3/metabolism ; Caspase 9/metabolism ; Caspase 9/pharmacology ; Cell Line, Tumor ; Curcumin/pharmacology ; Humans ; Myeloid Cell Leukemia Sequence 1 Protein/metabolism ; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma ; Proto-Oncogene Proteins c-bcl-2/metabolism ; Reactive Oxygen Species/metabolism ; Reactive Oxygen Species/pharmacology ; Sulfonamides ; Thioglycolates ; bcl-2-Associated X Protein/metabolism ; bcl-2-Associated X Protein/pharmacology
Chemical Substances	Apoptosis Regulatory Proteins ; Myeloid Cell Leukemia Sequence 1 Protein ; Proto-Oncogene Proteins c-bcl-2 ; Reactive Oxygen Species ; Sulfonamides ; Thioglycolates ; UMI-77 ; bcl-2-Associated X Protein ; Caspase 3 (EC 3.4.22.-) ; Caspase 9 (EC 3.4.22.-) ; Curcumin (IT942ZTH98)
Language	Chinese
Publishing date	2022-06-10
Publishing country	China
Document type	Journal Article
ZDB-ID	2404306-0
ISSN	1009-2137
ISSN	1009-2137
DOI	10.19746/j.cnki.issn.1009-2137.2022.03.006
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Article: A nomogram based on ultrasound scoring system for differentiating between immunoglobulin G4-related sialadenitis and primary Sjögren syndrome.

Su, Huan-Zhong / Hong, Long-Cheng / Huang, Mei / Zhang, Feng / Wu, Yu-Hui / Zhang, Zuo-Bing / Zhang, Xiao-Dong

Dento maxillo facial radiology

2024 Volume 53, Issue 1, Page(s) 43–51

Abstract: Objectives: Accurate distinguishing between immunoglobulin G4-related sialadenitis (IgG4-RS) and primary Sjögren syndrome (pSS) is crucial due to their different treatment approaches. This study aimed to construct and validate a nomogram based on the ... ...

Abstract	Objectives: Accurate distinguishing between immunoglobulin G4-related sialadenitis (IgG4-RS) and primary Sjögren syndrome (pSS) is crucial due to their different treatment approaches. This study aimed to construct and validate a nomogram based on the ultrasound (US) scoring system for the differentiation of IgG4-RS and pSS. Methods: A total of 193 patients with a clinical diagnosis of IgG4-RS or pSS treated at our institution were enrolled in the training cohort (n = 135; IgG4-RS = 28, pSS = 107) and the validation cohort (n = 58; IgG4-RS = 15, pSS = 43). The least absolute shrinkage and selection operator regression algorithm was utilized to screen the most optimal clinical features and US scoring parameters. A model for the differential diagnosis of IgG4-RS or pSS was built using logistic regression and visualized as a nomogram. The performance levels of the nomogram model were evaluated and validated in both the training and validation cohorts. Results: The nomogram incorporating clinical features and US scoring parameters showed better predictive value in differentiating IgG4-RS from pSS, with the area under the curves of 0.947 and 0.958 for the training cohort and the validation cohort, respectively. Decision curve analysis demonstrated that the nomogram was clinically useful. Conclusions: A nomogram based on the US scoring system showed favourable predictive efficacy in differentiating IgG4-RS from pSS. It has the potential to aid in clinical decision-making.
MeSH term(s)	Humans ; Sjogren's Syndrome/diagnostic imaging ; Nomograms ; Sialadenitis/diagnostic imaging ; Sialadenitis/drug therapy ; Immunoglobulin G/therapeutic use ; Diagnosis, Differential
Chemical Substances	Immunoglobulin G
Language	English
Publishing date	2024-01-12
Publishing country	England
Document type	Journal Article
ZDB-ID	221323-0
ISSN	1476-542X ; 0250-832X ; 0349-490X
ISSN (online)	1476-542X
ISSN	0250-832X ; 0349-490X
DOI	10.1093/dmfr/twad005
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Article ; Online: Neonatal Filaggrin Genetic Screening and Counseling to Prevent Atopic Dermatitis in High-Risk Infants.

Chen, Han-Ying / Chen, Chih-Ling / Wu, Yu-Hui / Lin, Tze-Kang / Su, Yi-Ning / Guo, Yue-Liang / Lin, Shin-Yu / Lee, Chien-Nan

Dermatitis : contact, atopic, occupational, drug

2024

Abstract: Background: ...

Abstract	Background:
Language	English
Publishing date	2024-04-02
Publishing country	United States
Document type	Journal Article
ZDB-ID	2144723-8
ISSN	2162-5220 ; 1532-8163 ; 1710-3568
ISSN (online)	2162-5220 ; 1532-8163
ISSN	1710-3568
DOI	10.1089/derm.2023.0233
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Article ; Online: Study found increasing use of core outcome sets in Cochrane systematic reviews and identified facilitators and barriers.

Saldanha, Ian J / Hughes, Karen L / Dodd, Susanna / Lasserson, Toby / Kirkham, Jamie J / Wu, Yuhui / Lucas, Samuel W / Williamson, Paula R

Journal of clinical epidemiology

2024 Volume 169, Page(s) 111277

Abstract: Objectives: In 2019, only 7% of Cochrane systematic reviews (SRs) cited a core outcome set (COS) in relation to choosing outcomes, even though a relevant COS existed but was not mentioned (or cited) for a further 29% of SRs. Our objectives for the ... ...

Abstract	Objectives: In 2019, only 7% of Cochrane systematic reviews (SRs) cited a core outcome set (COS) in relation to choosing outcomes, even though a relevant COS existed but was not mentioned (or cited) for a further 29% of SRs. Our objectives for the current work were to (1) examine the extent to which authors are currently considering COS to inform outcome choice in Cochrane protocols and completed SRs, and (2) understand author facilitators and barriers to using COS. Study design and setting: We examined all completed Cochrane SRs published in the last 3 months of 2022 and all Cochrane protocols published in 2022 for the extent to which they: (a) cited a COS, (b) searched for COS, (c) used outcomes from existing COS, and (d) reported outcome inconsistency among included studies and/or noted the need for COS. One investigator extracted information; a second extractor verified all information, discussing discrepancies to achieve consensus. We then conducted an online survey of authors of the included SRs to assess awareness of COS and identify facilitators and barriers to using COS to inform outcome choice. Results: Objective 1: We included 294 SRs of interventions (84 completed SRs and 210 published SR protocols), of which 13% cited specific COS and 5% did not cite but mentioned searching for COS. A median of 83% of core outcomes from cited COS (interquartile range [IQR] 57%-100%) were included in the corresponding SR. We identified a relevant COS for 39% of SRs that did not cite a COS. A median of 50% of core outcomes from noncited COS (IQR 35%-72%) were included in the corresponding SR. Objective 2: Authors of 236 (80%) of the 294 eligible SRs completed our survey. Seventy-seven percent of authors noted being aware of COS before the survey. Fifty-five percent of authors who did not cite COS but were aware of them reported searching for a COS. The most reported facilitators of using COS were author awareness of the existence of COS (59%), author positive perceptions of COS (52%), and recommendation in the Cochrane Handbook regarding COS use (48%). The most reported barriers related to matching of the scope of the COS and the SR: the COS target population was too narrow/broad relative to the SR population (29%) or the COS target intervention was too narrow/broad relative to the SR intervention (21%). Most authors (87%) mentioned that they would consider incorporating missing core outcomes in the SR/update. Conclusion: Since 2019, there is increasing consideration and awareness of COS when choosing outcomes for Cochrane SRs of interventions, but uptake remains low and can be improved further. Use of COS in SRs is important to improve outcome standardization, reduce research waste, and improve evidence syntheses of the relevant effects of interventions across health research.
Language	English
Publishing date	2024-02-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	639306-8
ISSN	1878-5921 ; 0895-4356
ISSN (online)	1878-5921
ISSN	0895-4356
DOI	10.1016/j.jclinepi.2024.111277
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Article: Risk factors for death associated with severe influenza in children and the impact of the COVID-19 pandemic on clinical characteristics.

Hu, Qian / Liang, Wen / Yi, Qiuwei / Zheng, Yuejie / Wang, Wenjian / Wu, Yuhui

Frontiers in pediatrics

2023 Volume 11, Page(s) 1249058

Abstract: Background: To summarize the clinical features of severe influenza in children and the high-risk factors for influenza-related deaths and to raise awareness among pediatricians.: Methods: A retrospective study of clinical manifestations, laboratory ... ...

Abstract	Background: To summarize the clinical features of severe influenza in children and the high-risk factors for influenza-related deaths and to raise awareness among pediatricians. Methods: A retrospective study of clinical manifestations, laboratory tests, and diagnosis and treatment of 243 children with severe influenza admitted to Shenzhen Children's Hospital from January 2009 to December 2022 was conducted. Univariate logistic regression analysis and Boruta analysis were also performed to identify potentially critical clinical characteristics associated with death, and clinically significant were used in further multivariate logistic regression analysis. Subject receiver operating characteristic (ROC) curves were applied to assess the efficacy of death-related independent risk factors to predict death from severe influenza. Results: There were 169 male and 74 female patients with severe influenza, with a median age of 3 years and 2 months and 77.4% of patients under six. There were 46 cases (18.9%) in the death group. The most common pathogen was Influenza A virus (IAV) (81.5%). The most common complication in the death group was influenza-associated acute necrotizing encephalopathy (ANE [52.2%]). Severe influenza in children decreased significantly during the COVID-19 pandemic, with a median age of 5 years, a high predominance of neurological symptoms such as ANE ( Conclusion: Neurological disorders such as ANE are more significant in children with severe influenza after the COVID-19 pandemic. Influenza virus infection can cause serious multisystem complications such as ARDS and ANE, and D-dimer has predictive value for early diagnosis and determination of the prognosis of children with severe influenza.
Language	English
Publishing date	2023-09-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2711999-3
ISSN	2296-2360
ISSN	2296-2360
DOI	10.3389/fped.2023.1249058
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Article ; Online: Synchronous Double Primary Malignant Tumors and their Possible Shared Genes: A Rare Clinical Entity.

Hu, Na / Yan, Gang / Tang, Mao-Wen / Wu, Yu-Hui / Xiang, Yi-Ning / Lei, Ping-Gui

Current medical imaging

2023 Volume 20, Page(s) e15734056258908

Abstract: Objective: This study sought to analyze the 18F-FDG PET/CT and contrast-enhanced computed tomography (CT) images of synchronous colorectal cancer (CRC) and renal clear cell carcinoma (ccRCC) and identify the shared genes between these two types of ... ...

Abstract	Objective: This study sought to analyze the 18F-FDG PET/CT and contrast-enhanced computed tomography (CT) images of synchronous colorectal cancer (CRC) and renal clear cell carcinoma (ccRCC) and identify the shared genes between these two types of cancer through bioinformatic analysis. Methods: A retrospective analysis was conducted on a patient with synchronous CRC and ccRCC who underwent 18F-FDG PET/CT and contrast-enhanced CT before treatment. Databases were analyzed to identify differentially expressed genes between CRC and ccRCC, and co-expression genes were extracted for RCC and CRC. Results: 18F-FDG PET/CT revealed intense metabolic activity in the primary colorectal lesion (SUVmax 13.2), while a left renal mass (diameter = 35 mm) was observed with no significant uptake. Contrast-enhanced CT during the arterial phase showed heterogeneous intense enhancement of the renal lesion, and the lesion washed out earlier than in the renal cortex in the nephrographic and excretory phases, indicating ccRCC. The histopathological results confirmed synchronous double primary malignant tumors. Our bioinformatic analysis results showed that synchronous occurrence of CRC and ccRCC may correlate with simultaneous expression of Carbonic Anhydrase 9 (CA9), integrin-binding sialoprotein (IBSP), and Fibrinogen γ chain (FGG). Conclusion: 18F-FDG PET/CT combined with contrast-enhanced CT is an effective diagnostic tool in evaluating synchronous CRC and RCC. By analyzing this clinical case and conducting bioinformatic analysis, we improved our current understanding of the mechanisms underlying synchronous tumors.
MeSH term(s)	Humans ; Carcinoma, Renal Cell/diagnostic imaging ; Carcinoma, Renal Cell/genetics ; Carcinoma, Renal Cell/pathology ; Fluorodeoxyglucose F18 ; Positron Emission Tomography Computed Tomography/methods ; Radiopharmaceuticals ; Retrospective Studies ; Kidney Neoplasms/diagnostic imaging ; Kidney Neoplasms/genetics ; Kidney Neoplasms/pathology
Chemical Substances	Fluorodeoxyglucose F18 (0Z5B2CJX4D) ; Radiopharmaceuticals
Language	English
Publishing date	2023-12-12
Publishing country	United Arab Emirates
Document type	Journal Article
ISSN	1573-4056
ISSN (online)	1573-4056
DOI	10.2174/0115734056258908230920063040
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Article: Whole-Genome Analysis of Termite-Derived

Zhang, Xingbo / He, Xiaotao / Chen, Jieru / Li, Jingtao / Wu, Yuhui / Chen, Yu / Yang, Yuhui

Microorganisms

2023 Volume 11, Issue 11

Abstract: ... Bacillus ... ...

Abstract	Bacillus velezensis
Language	English
Publishing date	2023-11-03
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms11112697
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