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  1. Article ; Online: Threadlike Linear Calcifications along the Proximal Colon of a 71-Year-Old Woman with Chronic Abdominal Pain.

    Le, Puo-Hsien / Chen, Chien-Ming / Wu, Ren-Chin

    Gastrointestinal endoscopy

    2024  

    Language English
    Publishing date 2024-04-29
    Publishing country United States
    Document type Journal Article
    ZDB-ID 391583-9
    ISSN 1097-6779 ; 0016-5107
    ISSN (online) 1097-6779
    ISSN 0016-5107
    DOI 10.1016/j.gie.2024.04.2908
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    Zs.MO 519: Show issues

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  2. Article ; Online: A Hairy Pedunculated Polypoid Lesion of Sigmoid Colon in a Female with Abdominal Pain and Diarrhea.

    Le, Puo-Hsien / Tsou, Yung-Kuan / Wu, Ren-Chin

    Gastrointestinal endoscopy

    2024  

    Language English
    Publishing date 2024-03-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 391583-9
    ISSN 1097-6779 ; 0016-5107
    ISSN (online) 1097-6779
    ISSN 0016-5107
    DOI 10.1016/j.gie.2024.03.014
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  3. Article ; Online: Image processing based on the detection of external defects of fan tower Weld

    Wu Ren / Sun Yuan

    E3S Web of Conferences, Vol 252, p

    2021  Volume 02026

    Abstract: With the continuous development of wind power generation technology and the continuous increase in the demand for electric energy, the height of the fan tower is more and more demanding. It is very important to detect the weld produced in the welding ... ...

    Abstract With the continuous development of wind power generation technology and the continuous increase in the demand for electric energy, the height of the fan tower is more and more demanding. It is very important to detect the weld produced in the welding process of fan tower. In this paper, an algorithm for weld defect detection based on traditional image processing and convolutional neural network is proposed. Firstly, the traditional image processing algorithm is used to gray the weld image collected by industrial camera. Then, the gray image of welding seam is enhanced to improve the visual effect and clear the image, which is convenient for further processing and analysis of the image by computer. Finally, the image is used as the input of the trained convolution neural network to judge whether there are defects outside the weld.
    Keywords Environmental sciences ; GE1-350
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher EDP Sciences
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Digenic CHD7 and SMCHD1 inheritance Unveils phenotypic variability in a family mainly presenting with hypogonadotropic hypogonadism

    Tian Wang / Wu Ren / Fangfang Fu / Hairong Wang / Yan Li / Jie Duan

    Heliyon, Vol 10, Iss 1, Pp e23272- (2024)

    1481  

    Abstract: Objectives: CHARGE syndrome is a congenital hereditary condition involving multiple systems. Patients are easily misdiagnosed with idiopathic hypogonadotropic hypogonadism (IHH) due to the overlap of clinical manifestations. An accurate clinical ... ...

    Abstract Objectives: CHARGE syndrome is a congenital hereditary condition involving multiple systems. Patients are easily misdiagnosed with idiopathic hypogonadotropic hypogonadism (IHH) due to the overlap of clinical manifestations. An accurate clinical diagnosis remains challenging when the predominant clinical manifestation resembles hypogonadotropic hypogonadism. Methods: This original research is conducted based on the genetic finding and analysis of clinical cases. Whole-exome sequencing (WES) and in-silico analyse were performed on two sisters to investigate the pathogenesis in this family. Homology modelling was conducted to evaluate structural changes in the variants. Results: WES and Sanger sequencing revealed two siblings carrying a nonsense mutation (NM_017780.4: c.115C > T) in exon 2 of CHD7 inherited from a mildly affected mother and a missense mutation (NM_015295.3: c.2582T > C) in exon 20 of SMCHD1 inherited from an asymptomatic father. The nonsense mutation in CHD7 was predicted to generate nonsense-mediated decay, whereas the missense mutation in SMCHD1 decreased protein stability. Conclusions: We identified digenic CHD7 and SMCHD1 mutations in IHH-associated diseases for the first time and verified the synergistic role of oligogenic inheritance. It was also determined that WES is an effective tool for distinguishing diseases with overlapping features and establishing a molecular diagnosis for cases with digenic or oligogenic hereditary disorders, which is beneficial for timely treatment, and family genetic counseling.
    Keywords CHARGE syndrome ; Idiopathic hypogonadotropic hypogonadism ; Intrafamilial phenotypic variability ; CHD7 ; SMCHD1 ; Oligogenicity ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
    Subject code 610
    Language English
    Publishing date 2024-01-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Book ; Thesis: Berechnungen des regionalen relativen Blutvolumens mittels MRT bei Patienten mit intracraniellem Meningeom, cerebralen Ischämien und HNO-Tumoren

    Wu, Ren Hua

    Applikation des ersten Bolusdurchganges

    1997  

    Author's details vorgelegt von Ren Hua Wu
    Language German
    Size 107 S. : Ill., graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis München, Univ., Diss., 1997
    HBZ-ID HT008276848
    Database Catalogue ZB MED Medicine, Health

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  6. Article ; Online: Identification and genetic engineering of pneumococcal capsule-like polysaccharides in commensal oral streptococci.

    Wu, Ren / Nahm, Moon / Yang, Jinghua / Bush, C Allen / Wu, Hui

    Microbiology spectrum

    2024  Volume 12, Issue 4, Page(s) e0188523

    Abstract: Capsular polysaccharides (CPS) in : Importance: Diverse capsules ... ...

    Abstract Capsular polysaccharides (CPS) in
    Importance: Diverse capsules from
    MeSH term(s) Streptococcus pneumoniae/genetics ; Streptococcus/genetics ; Polysaccharides ; Serogroup ; Pneumococcal Vaccines ; Genetic Engineering ; Bacterial Capsules ; Polysaccharides, Bacterial
    Chemical Substances Polysaccharides ; Pneumococcal Vaccines ; Polysaccharides, Bacterial
    Language English
    Publishing date 2024-03-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.01885-23
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  7. Article ; Online: MiR-181a-5p promotes osteogenesis by targeting BMP3.

    Long, Ze / Dou, Pengcheng / Cai, Weiliang / Mao, Minzhi / Wu, Ren

    Aging

    2023  Volume 15, Issue 3, Page(s) 734–747

    Abstract: High-throughput microRNA (miRNA) sequencing of osteoporosis was analyzed from the Gene Expression Omnibus (GEO) database to investigate specific microRNAs that control osteogenesis. MiR-181a-5p was differentially expressed among healthy subjects and ... ...

    Abstract High-throughput microRNA (miRNA) sequencing of osteoporosis was analyzed from the Gene Expression Omnibus (GEO) database to investigate specific microRNAs that control osteogenesis. MiR-181a-5p was differentially expressed among healthy subjects and those with osteoporosis. Inhibitors and mimics were transfected into cells to modulate miR-181a-5p levels to examine the role in MC3T3-E1 functions. Alkaline phosphatase (ALP) staining and Alizarin Red S (ARS) staining were used for morphological detection, and proteins of ALP and Runt-related transcription factor 2 (RUNX2), as osteogenesis markers, were detected. During the osteogenic differentiation of MC3T3-E1, the transcription level of miR-181a-5p was significantly increased. The inhibition of miR-181a-5p suppressed MC3T3-E1 osteogenic differentiation, whereas its overexpression functioned oppositely. Consistently, the miR-181a-5p antagomir aggravated osteoporosis in old mice. Additionally, we predicted potential target genes via TargetScan and miRDB and identified bone morphogenetic protein 3 (BMP3) as the target gene. Moreover, the reduced expression of miR-181a-5p was validated in our hospitalized osteoporotic patients. These findings have substantial implications for the strategies targeting miR-181a-5p to prevent osteoporosis and potential related fractures.
    MeSH term(s) Mice ; Animals ; Osteogenesis/genetics ; Bone Morphogenetic Protein 3 ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Cell Differentiation/genetics ; Osteoporosis/genetics ; Osteoporosis/metabolism
    Chemical Substances Bone Morphogenetic Protein 3 ; MicroRNAs
    Language English
    Publishing date 2023-02-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1945-4589
    ISSN (online) 1945-4589
    DOI 10.18632/aging.204505
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  8. Article ; Online: A highly efficient and accurate method of detecting and subtyping Influenza A pdm H1N1 and H3N2 viruses with newly emerging mutations in the matrix gene in Eastern Taiwan.

    Yang, Hui-Hua / Huang, I-Tsong / Wu, Ren-Chieh / Chen, Li-Kuang

    PloS one

    2023  Volume 18, Issue 3, Page(s) e0283074

    Abstract: The rapid identification of Influenza A virus and its variants, which cause severe respiratory diseases, is imperative to providing timely treatment and improving patient outcomes. Conventionally, two separate assays (total test duration of up to 6 h) ... ...

    Abstract The rapid identification of Influenza A virus and its variants, which cause severe respiratory diseases, is imperative to providing timely treatment and improving patient outcomes. Conventionally, two separate assays (total test duration of up to 6 h) are required to initially differentiate Influenza A and B viruses and subsequently distinguish the pdm H1N1 and H3N2 serotypes of Influenza A virus. In this study, we developed a multiplex real-time RT-PCR method for simultaneously detecting Influenza A and B viruses and subtyping Influenza A virus, with a substantially reduced test duration. Clinical specimens from hospitalized patients and outpatients with influenza-like symptoms in Eastern Taiwan were collected between 2011 and 2015, transported to Hualien Tzu Chi Hospital, and analyzed. Conventional RT-PCR was used to subtype the isolated Influenza A viruses. Thereafter, for rapid identification, the multiplex real-time RT-PCR method was developed and applied to identify the conserved regions that aligned with the available primers and probes. Accordingly, a multiplex RT-PCR assay with three groups of primers and probes (MAF and MAR primers and MA probe; InfAF and InfAR primers and InfA probe; and MBF and MBR primers and MB probe) was established to distinguish these viruses in the same reaction. Thus, with this multiplex RT-PCR assay, Influenza B, Influenza A pdm H1N1, and Influenza A H3N2 viruses were accurately detected and differentiated within only 2.5 h. This multiplex RT-PCR assay showed similar analytical sensitivity to the conventional singleplex assay. Further, the phylogenetic analyses of our samples revealed that the characteristics of these viruses were different from those reported previously using samples collected during 2012-2013. In conclusion, we developed a multiplex real-time RT-PCR method for highly efficient and accurate detection and differentiation of Influenza A and B viruses and subtyping Influenza A virus with a substantially reduced test duration for diagnosis.
    MeSH term(s) Humans ; Influenza, Human/diagnosis ; Influenza A Virus, H3N2 Subtype ; Influenza A Virus, H1N1 Subtype ; Taiwan ; Phylogeny ; Sensitivity and Specificity ; Influenza A virus/genetics ; Mutation ; DNA Primers/genetics ; Reverse Transcriptase Polymerase Chain Reaction
    Chemical Substances DNA Primers
    Language English
    Publishing date 2023-03-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0283074
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  9. Article ; Online: Bamboo Joint-Like Appearance of the Stomach in a 59-Year-Old Man With Chronic Abdominal Pain.

    Le, Puo-Hsien / Chang, Chen-Wang / Chen, Chien-Ming / Wu, Ren-Chin

    The American journal of gastroenterology

    2023  Volume 118, Issue 11, Page(s) 1922

    MeSH term(s) Male ; Humans ; Middle Aged ; Stomach/diagnostic imaging ; Abdominal Pain/etiology ; Upper Gastrointestinal Tract ; Abdomen
    Language English
    Publishing date 2023-05-19
    Publishing country United States
    Document type Case Reports ; Journal Article
    ZDB-ID 390122-1
    ISSN 1572-0241 ; 0002-9270
    ISSN (online) 1572-0241
    ISSN 0002-9270
    DOI 10.14309/ajg.0000000000002324
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  10. Article ; Online: Boronic acid and fructose-1,6-diphosphate dual-functionalized highly hydrophilic Zr-MOF for HILIC enrichment of N-linked glycopeptides.

    Zhou, Xiaoyu / Zhang, Hongyan / Wang, Li / Wu, Ren'an

    Analytical and bioanalytical chemistry

    2023  Volume 415, Issue 19, Page(s) 4767–4777

    Abstract: The HILIC enrichment is a greatly compatible strategy for the extraction of glycopeptides in proteomics. Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the ... ...

    Abstract The HILIC enrichment is a greatly compatible strategy for the extraction of glycopeptides in proteomics. Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration. A total of 359 N-linked glycopeptides corresponding to 104 glycoproteins were identified from only 1 μL of digested human serum by the enrichment of UIO-PBA&FDP, which showed a superiorly high coverage of the identified glycopeptides. The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
    MeSH term(s) Humans ; Glycopeptides/chemistry ; Glycoproteins ; Hydrophobic and Hydrophilic Interactions ; Boronic Acids/chemistry
    Chemical Substances Glycopeptides ; fructose-1,6-diphosphate (M7522JYX1H) ; Glycoproteins ; Boronic Acids
    Language English
    Publishing date 2023-06-05
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 201093-8
    ISSN 1618-2650 ; 0016-1152 ; 0372-7920
    ISSN (online) 1618-2650
    ISSN 0016-1152 ; 0372-7920
    DOI 10.1007/s00216-023-04770-z
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