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Artikel: 2,4-Dichlorophenoxyacetic Acid Induces Degeneration of mDA Neurons In Vitro.

Russ, Tamara / Enders, Lennart / Zbiegly, Julia M / Potru, Phani Sankar / Wurm, Johannes / Spittau, Björn

2023 Band 11, Heft 11

Abstract: Background: ...

Abstract	Background:
Sprache	Englisch
Erscheinungsdatum	2023-10-24
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article
ZDB-ID	2720867-9
ISSN	2227-9059
ISSN	2227-9059
DOI	10.3390/biomedicines11112882
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Buch: SPEKTROSKOPISCHE CHLORBESTIMMUNGEN IM SCHMELZ UND DENTIN MENSCHLICHER ZAEHNE

Wurm, Johannes

1979

Umfang	118 S.
Dokumenttyp	Buch
Anmerkung	GIESSEN, UNIV., FACHBEREICH 23 - HUMANMEDIZIN, DISS., 1980
HBZ-ID	HT002596555
Datenquelle	Katalog ZB MED Medizin, Gesundheit

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Artikel ; Online: Microglia Development and Maturation and Its Implications for Induction of Microglia-Like Cells from Human iPSCs.

Wurm, Johannes / Konttinen, Henna / Andressen, Christian / Malm, Tarja / Spittau, Björn

International journal of molecular sciences

2021 Band 22, Heft 6

Abstract: Microglia are resident immune cells of the central nervous system and play critical roles during the development, homeostasis, and pathologies of the brain. Originated from yolk sac erythromyeloid progenitors, microglia immigrate into the embryonic brain ...

Abstract	Microglia are resident immune cells of the central nervous system and play critical roles during the development, homeostasis, and pathologies of the brain. Originated from yolk sac erythromyeloid progenitors, microglia immigrate into the embryonic brain parenchyma to undergo final postnatal differentiation and maturation driven by distinct chemokines, cytokines, and growth factors. Among them, TGFβ1 is an important regulator of microglial functions, mediating homeostasis, anti-inflammation, and triggering the expression of microglial homeostatic signature genes. Since microglia studies are mainly based on rodent cells and the isolation of homeostatic microglia from human tissue is challenging, human-induced pluripotent stem cells have been successfully differentiated into microglia-like cells recently. However, employed differentiation protocols strongly vary regarding used cytokines and growth factors, culture conditions, time span, and cell yield. Moreover, the incomplete differentiation of human microglia can hamper the similarity to primary human microglia and dramatically influence the outcome of follow-up studies with these differentiated cells. This review summarizes the current knowledge of the molecular mechanisms driving rodent microglia differentiation in vivo, further compares published differentiation protocols, and highlights the potential of TGFβ as an essential maturation factor.
Mesh-Begriff(e)	Animals ; Cell Differentiation ; Cell-Matrix Junctions/metabolism ; Humans ; Induced Pluripotent Stem Cells/cytology ; Microglia/cytology ; Microglia/metabolism ; Models, Biological ; Transforming Growth Factor beta/metabolism
Chemische Substanzen	Transforming Growth Factor beta
Sprache	Englisch
Erscheinungsdatum	2021-03-17
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms22063088
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Suppression of TGF-β pathway by pirfenidone decreases extracellular matrix deposition in ocular fibroblasts in vitro.

Stahnke, Thomas / Kowtharapu, Bhavani S / Stachs, Oliver / Schmitz, Klaus-Peter / Wurm, Johannes / Wree, Andreas / Guthoff, Rudolf Friedrich / Hovakimyan, Marina

PloS one

2017 Band 12, Heft 2, Seite(n) e0172592

Abstract: In glaucoma surgery, fibrotic processes occur, leading to impairment of liquid outflow. Activated fibroblasts are responsible for postoperative scarring. The transforming growth factor-β (TGF-β) pathway plays a key role in fibroblast function, ... ...

Abstract	In glaucoma surgery, fibrotic processes occur, leading to impairment of liquid outflow. Activated fibroblasts are responsible for postoperative scarring. The transforming growth factor-β (TGF-β) pathway plays a key role in fibroblast function, differentiation and proliferation. The aim of this study was the characterization of the fibrotic potential of two subtypes of primary human ocular fibroblasts and the attempt to inhibit fibrotic processes specifically, without impairing cell viability. For fibrosis inhibition we focused on the small molecule pirfenidone, which has been shown to prevent pulmonary fibrosis by the decrease of the expression of TGF-β1, TGF-β2 and TGF-β3 cytokines. For in vitro examinations, isolated human primary fibroblasts from Tenon capsule and human intraconal orbital fat tissues were used. These fibroblast subpopulations were analyzed in terms of the expression of matrix components responsible for postoperative scarring. We concentrated on the expression of collagen I, III, VI and fibronectin. Additionally, we analyzed the expression of α-smooth muscle actin, which serves as a marker for fibrosis and indicates transformation of fibroblasts into myofibroblasts. Gene expression was analyzed by rtPCR and synthesized proteins were examined by immunofluorescence and Western blot methods. Proliferation of fibroblasts under different culture conditions was assessed using BrdU assay. TGF-β1 induced a significant increase of cell proliferation in both cell types. Also the expression of some fibrotic markers was elevated. In contrast, pirfenidone decreased cell proliferation and matrix synthesis in both fibroblast subpopulations. Pirfenidone slightly attenuated TGF-β1 induced expression of fibronectin and α-smooth muscle actin in fibroblast cultures, without impairing cell viability. To summarize, manipulation of the TGF-β signaling pathway by pirfenidone represents a specific antifibrotic approach with no toxic side effects in two human orbital fibroblast subtypes. We presume that pirfenidone is a promising candidate for the treatment of fibrosis following glaucoma surgery.
Mesh-Begriff(e)	Actins/analysis ; Actins/genetics ; Anti-Inflammatory Agents, Non-Steroidal/pharmacology ; Cell Proliferation/drug effects ; Cells, Cultured ; Extracellular Matrix/drug effects ; Extracellular Matrix/genetics ; Extracellular Matrix/metabolism ; Extracellular Matrix/pathology ; Fibroblasts/cytology ; Fibroblasts/drug effects ; Fibroblasts/metabolism ; Fibroblasts/pathology ; Fibronectins/analysis ; Fibronectins/genetics ; Fibrosis ; Gene Expression Regulation/drug effects ; Humans ; Pyridones/pharmacology ; Signal Transduction/drug effects ; Transforming Growth Factor beta/antagonists & inhibitors ; Transforming Growth Factor beta/metabolism
Chemische Substanzen	ACTA2 protein, human ; Actins ; Anti-Inflammatory Agents, Non-Steroidal ; Fibronectins ; Pyridones ; Transforming Growth Factor beta ; pirfenidone (D7NLD2JX7U)
Sprache	Englisch
Erscheinungsdatum	2017-02-23
Erscheinungsland	United States
Dokumenttyp	Journal Article
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0172592
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Buch ; Dissertation / Habilitation: Spektroskopische Chlorbestimmungen im Schmelz und Dentin menschlicher Zähne

Wurm, Johannes

1979

Verfasserangabe	vorgelegt von Johannes Wurm
Sprache	Deutsch
Umfang	118 S
Dokumenttyp	Buch ; Dissertation / Habilitation
Dissertation / Habilitation	@Giessen, Univ., Diss. : 1980
Datenquelle	Ehemaliges Sondersammelgebiet Küsten- und Hochseefischerei

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Artikel ; Online: Predictors for hospitalization and outpatient visits in patients with inflammatory bowel disease: results from the Swiss Inflammatory Bowel Disease Cohort Study.

Sulz, Michael C / Siebert, Uwe / Arvandi, Marjan / Gothe, Raffaella M / Wurm, Johannes / von Känel, Roland / Vavricka, Stephan R / Meyenberger, Christa / Sagmeister, Markus

European journal of gastroenterology & hepatology

2013 Band 25, Heft 7, Seite(n) 790–797

Abstract: Objectives: Patients with inflammatory bowel disease (IBD) have a high resource consumption, with considerable costs for the healthcare system. In a system with sparse resources, treatment is influenced not only by clinical judgement but also by ... ...

Abstract	Objectives: Patients with inflammatory bowel disease (IBD) have a high resource consumption, with considerable costs for the healthcare system. In a system with sparse resources, treatment is influenced not only by clinical judgement but also by resource consumption. We aimed to determine the resource consumption of IBD patients and to identify its significant predictors. Materials and methods: Data from the prospective Swiss Inflammatory Bowel Disease Cohort Study were analysed for the resource consumption endpoints hospitalization and outpatient consultations at enrolment [1187 patients; 41.1% ulcerative colitis (UC), 58.9% Crohn's disease (CD)] and at 1-year follow-up (794 patients). Predictors of interest were chosen through an expert panel and a review of the relevant literature. Logistic regressions were used for binary endpoints, and negative binomial regressions and zero-inflated Poisson regressions were used for count data. Results: For CD, fistula, use of biologics and disease activity were significant predictors for hospitalization days (all P-values <0.001); age, sex, steroid therapy and biologics were significant predictors for the number of outpatient visits (P=0.0368, 0.023, 0.0002, 0.0003, respectively). For UC, biologics, C-reactive protein, smoke quitters, age and sex were significantly predictive for hospitalization days (P=0.0167, 0.0003, 0.0003, 0.0076 and 0.0175 respectively); disease activity and immunosuppressive therapy predicted the number of outpatient visits (P=0.0009 and 0.0017, respectively). The results of multivariate regressions are shown in detail. Conclusion: Several highly significant clinical predictors for resource consumption in IBD were identified that might be considered in medical decision-making. In terms of resource consumption and its predictors, CD and UC show a different behaviour.
Mesh-Begriff(e)	Adult ; Ambulatory Care/utilization ; Colitis, Ulcerative/diagnosis ; Colitis, Ulcerative/epidemiology ; Colitis, Ulcerative/therapy ; Crohn Disease/diagnosis ; Crohn Disease/epidemiology ; Crohn Disease/therapy ; Female ; Health Resources/utilization ; Hospitalization ; Humans ; Logistic Models ; Male ; Middle Aged ; Multivariate Analysis ; Odds Ratio ; Prospective Studies ; Referral and Consultation/utilization ; Risk Factors ; Switzerland/epidemiology ; Time Factors
Sprache	Englisch
Erscheinungsdatum	2013-07
Erscheinungsland	England
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1034239-4
ISSN	1473-5687 ; 0954-691X
ISSN (online)	1473-5687
ISSN	0954-691X
DOI	10.1097/MEG.0b013e32836019b9
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Predictors of temporary and permanent work disability in patients with inflammatory bowel disease: results of the swiss inflammatory bowel disease cohort study.

Siebert, Uwe / Wurm, Johannes / Gothe, Raffaella Matteucci / Arvandi, Marjan / Vavricka, Stephan R / von Känel, Roland / Begré, Stefan / Sulz, Michael C / Meyenberger, Christa / Sagmeister, Markus

Inflammatory bowel diseases

2012 Band 19, Heft 4, Seite(n) 847–855

Abstract: Background: Inflammatory bowel disease can decrease the quality of life and induce work disability. We sought to (1) identify and quantify the predictors of disease-specific work disability in patients with inflammatory bowel disease and (2) assess the ... ...

Abstract	Background: Inflammatory bowel disease can decrease the quality of life and induce work disability. We sought to (1) identify and quantify the predictors of disease-specific work disability in patients with inflammatory bowel disease and (2) assess the suitability of using cross-sectional data to predict future outcomes, using the Swiss Inflammatory Bowel Disease Cohort Study data. Methods: A total of 1187 patients were enrolled and followed up for an average of 13 months. Predictors included patient and disease characteristics and drug utilization. Potential predictors were identified through an expert panel and published literature. We estimated adjusted effect estimates with 95% confidence intervals using logistic and zero-inflated Poisson regression. Results: Overall, 699 (58.9%) experienced Crohn's disease and 488 (41.1%) had ulcerative colitis. Most important predictors for temporary work disability in patients with Crohn's disease included gender, disease duration, disease activity, C-reactive protein level, smoking, depressive symptoms, fistulas, extraintestinal manifestations, and the use of immunosuppressants/steroids. Temporary work disability in patients with ulcerative colitis was associated with age, disease duration, disease activity, and the use of steroids/antibiotics. In all patients, disease activity emerged as the only predictor of permanent work disability. Comparing data at enrollment versus follow-up yielded substantial differences regarding disability and predictors, with follow-up data showing greater predictor effects. Conclusions: We identified predictors of work disability in patients with Crohn's disease and ulcerative colitis. Our findings can help in forecasting these disease courses and guide the choice of appropriate measures to prevent adverse outcomes. Comparing cross-sectional and longitudinal data showed that the conduction of cohort studies is inevitable for the examination of disability.
Mesh-Begriff(e)	Adult ; Colitis, Ulcerative/diagnosis ; Colitis, Ulcerative/epidemiology ; Colitis, Ulcerative/psychology ; Crohn Disease/diagnosis ; Crohn Disease/epidemiology ; Crohn Disease/psychology ; Disabled Persons/psychology ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Predictive Value of Tests ; Prospective Studies ; Switzerland/epidemiology ; Work Capacity Evaluation
Sprache	Englisch
Erscheinungsdatum	2012-11-09
Erscheinungsland	England
Dokumenttyp	Journal Article ; Multicenter Study ; Research Support, Non-U.S. Gov't
ZDB-ID	1340971-2
ISSN	1536-4844 ; 1078-0998
ISSN (online)	1536-4844
ISSN	1078-0998
DOI	10.1097/MIB.0b013e31827f278e
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Zeitschrift: Clevere Kombination. Kohlevergasung, Roheisenerzeugung und GuD-Kraftwerk

Koch, Peter / Leonard, Guido / Siuka, Dieter / Wurm, Johannes / Harbison, Edwin

Energie Spektrum : Technik, Management, Wirtschaft, Politik

1995 Band 10, Seite(n) 23–24, 26, 28, 30

Abstract: Darstellung des zukunftsweisenden vom Voest-Alpine-Konzern entwickelten Corex-Prozesses, der die Erzeugung von Roheisen und Synthesegas koppelt, das im GuD-Kraftwerk zur Stromerzeugung genutzt wird. Beim Corex-Prozess wird kein Koks mehr benoetigt und ... ...

Abstract	Darstellung des zukunftsweisenden vom Voest-Alpine-Konzern entwickelten Corex-Prozesses, der die Erzeugung von Roheisen und Synthesegas koppelt, das im GuD-Kraftwerk zur Stromerzeugung genutzt wird. Beim Corex-Prozess wird kein Koks mehr benoetigt und auch die Gasentschwefelung ist nicht mehr erforderlich. Seit 1989 ist in Suedafrika eine industrielle Grossanlage erfolgreich in Betrieb. Die optimale Nutzung des Exportgases in einer nachgeschalteten GuD-Anlage wird beschrieben. Die Wirtschaftlichkeit in diesem Produktionsverbund wird nachgewiesen. Sowohl CO2 und Luftschadstoffe (wie NOx, SO2 und Staub) als auch Abwasserschadstoffe (wie Phenole, Sulfide, Ammonium und freie Zyanide) sind in den Emissionen des Corex-Prozesses gegenueber der konventionellen Hochofenroute nur mehr in Bruchteilen vorhanden. Dieses ist zukunftsbezogen sicher einer der entscheidenden Erfolgsfaktoren fuer Corex.
Schlagwörter	Eisenhuettenindustrie ; Kohlevergasung ; Synthesegas ; Verfahrenskombination ; Anlagenbeschreibung ; Gasreinigung ; Entschwefelung ; Umweltvertraeglichkeit ; Emissionsminderung ; Schwefeldioxid ; Stickstoffoxid ; Staub ; Schadstoffminderung ; Abwasserbeschaffenheit ; Phenol ; Sulfid ; Cyanid ; Verfahrensvergleich ; Verfahrenstechnik ; Elektrizitaetserzeugung ; Koks ; Wirtschaftlichkeit ; Hochofen ; Sauerstoff ; Kohlendioxid ; Aussenhandel ; Schadstoffemission ; Ammonium ; GuD-Kraftwerk
Sprache	Deutsch
Dokumenttyp	Zeitschrift
Datenquelle	OPAC und UmweltLIteraturDATenbank (ULIDAT) des Umweltbundesamtes (UBA)

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Buch ; Dissertation / Habilitation: Über die Giftigkeit von Nebennierenrinden-Auszügen

Wurm, Johannes

1925

Verfasserangabe	von Johannes Wurm
Sprache	Deutsch
Umfang	41 gez. Bl
Erscheinungsort	Hamburg
Dokumenttyp	Buch ; Dissertation / Habilitation
Dissertation / Habilitation	Univ., Diss. med.--Hamburg, 1925
Datenquelle	Ehemaliges Sondersammelgebiet Küsten- und Hochseefischerei

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Artikel: Mixed forests and a flexible harvest policy: a problem for conventional risk analysis?

Knoke, Thomas / Wurm, Johannes

European journal of forest research

Band v. 125,, Heft no. 3

Abstract: The famous “Faustmann” equation, which allows for identifying the most profitable tree species on a given unstocked piece of land, assumes constant timber prices. In reality, timber prices may fluctuate dramatically. Several authors have proven for ... ...

Abstract	The famous “Faustmann” equation, which allows for identifying the most profitable tree species on a given unstocked piece of land, assumes constant timber prices. In reality, timber prices may fluctuate dramatically. Several authors have proven for monocultures that waiting for an acceptable timber price (reservation price) before harvesting (flexible harvest policy) increases the net present value of forest management. The first part of this paper investigates how efficient a flexible harvest strategy may be applied in mixed forests and whether the optimal species mixture is changed under such harvest policy. Mixtures of the conifer Norway spruce [Picea abies (L.) Karst] and the broadleaf European beech (Fagus sylvatica L.) were investigated. In order to evaluate mixed forests, the risks and the correlation of risks between tree species as well as the attitude towards risk of the decision-maker (risk-aversion is assumed) were considered according to the classical theory of optimal portfolio selection. In the second part we took up a recent critique on modern financial theory by Mandelbrot. Whether or not the assumption of normally distributed financial flows, which are supposed to occur under risk, would be appropriate to evaluate the risk of forest management was investigated. Market and hazard risks as well as their correlation were integrated in the evaluation of mixed forests by means of Monte-Carlo simulations (MCS). The risk of the timber price fluctuation was combined with the natural hazard risk, caused mainly by insects, snow and wind. Applying the μ-σ-rule, the mean net present value (NPV) from 1,000 simulations and their standard deviation were used for the optimisation. Given a low-return, risk-free interest rate to assess potential species mixtures of the Norway spruce and European beech, optimal proportions of European beech increased according to the theory of optimum portfolio selection with growing risk aversion from 0 (ignorance of risk) to 60% (great risk-aversion). In relation to a fixed harvest policy, the net present value of both, Norway spruce and European beech, could be increased significantly. Since the hazard risks of European beech were substantially lower compared with the Norway spruce (relation of susceptibility 1:4) beech benefited more from the flexible harvest policy. A comparison of simulated frequency distributions of the NPV with the expected density functions under the assumption of a normal distribution revealed significant differences. Only in the case of European beech was the general shape of the simulated frequency distribution similar to a normal distribution (bell-shaped curve). However, the density of NPV close to the mean was much greater than expected under the assumption of a normal distribution. Consequently, the frequency of a negative NPV for a European beech forest was greatly overestimated when applying the normal distribution. Though the shape of the simulated frequency distribution was rather different from a normal distribution for Norway spruce the simulated part of negative NPV was quite well approximated by the normal distribution. Therefore the simulated and expected frequencies of negative NPV were similar in case of Norway spruce; only a slight underestimation was seen in the assumption of a normal distribution. It can be concluded that actually simulated frequencies of negative NPV seem to be better measures for risk than computed probabilities of negative NPV, which assume normal distribution. As the risk for European beech was greatly overestimated by the conventional assumption of a normal distribution, the optimal proportions of European beech were surely rather underestimated according to the theory of portfolio. MCS on optimum mixtures derived by the classical portfolio theory seems necessary to test the robustness of such mixtures.
Schlagwörter	wind ; markets ; Fagus sylvatica subsp. sylvatica ; Monte Carlo method ; equations ; insects ; conifers ; risk ; forest management ; mixed forests ; economic theory ; normal distribution ; harvesting ; snow ; issues and policy ; trees ; prices ; Picea abies ; risk analysis
Sprache	Englisch
Dokumenttyp	Artikel
ISSN	1612-4669
Datenquelle	AGRIS - International Information System for the Agricultural Sciences and Technology

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