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Article ; Online: Using cryoEM and cryoET to visualize membrane penetration of a non-enveloped virus

Xian Xia / Z. Hong Zhou

STAR Protocols, Vol 3, Iss 4, Pp 101825- (2022)

2022

Abstract: Summary: Key to cell entry by non-enveloped viruses is virus-cell interactions at the cell or endosomal membrane. Here, we detail our protocols to capture such interactions between non-enveloped virus bluetongue virus (BTV) and vesicular membrane by ... ...

Abstract	Summary: Key to cell entry by non-enveloped viruses is virus-cell interactions at the cell or endosomal membrane. Here, we detail our protocols to capture such interactions between non-enveloped virus bluetongue virus (BTV) and vesicular membrane by cryogenic electron microscopy (cryoEM) and tomography (cryoET). Key steps include virus isolation, liposome preparation, virus-liposome incubation and vitrification, cryoEM and cryoET imaging, data processing for 3D reconstruction, and subtomogram averaging. The protocols can be generally applicable to studies of cell entry by other non-enveloped viruses.For complete details on the use and execution of this protocol, please refer to Xia et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Keywords	Microbiology ; Microscopy ; Structural biology ; Cryo-EM ; Science (General) ; Q1-390
Language	English
Publishing date	2022-12-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
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Article ; Online: Developing and evaluating an interprofessional shared decision-making care model for patients with perinatal depression in maternal care in urban China

Defang Xiang / Xian Xia / Di Liang

BMC Primary Care, Vol 24, Iss 1, Pp 1-

a study protocol

2023 Volume 13

Abstract: Abstract Background The majority of patients with perinatal depression (PND) in China do not receive adequate treatment. As forming a therapeutic alliance with patients is crucial for depression treatment, shared decision-making (SDM) shows promise in ... ...

Abstract	Abstract Background The majority of patients with perinatal depression (PND) in China do not receive adequate treatment. As forming a therapeutic alliance with patients is crucial for depression treatment, shared decision-making (SDM) shows promise in promoting patients’ uptake of evidence-based mental health services, but its impact on patient outcomes and implementation in real-world maternal care remain uncertain. Therefore, this study aims to develop and evaluate an interprofessional shared decision-making (IP-SDM) model for PND to enhance maternal mental health services. Methods This study contains four research phases: feasibility testing (Phase 1), toolkit development (Phase 2), usability evaluation (Phase 3), and effectiveness evaluation (Phase 4). During the development stage, focus group interviews will be conducted with expectant and new mothers, as well as maternal care providers for feasibility testing. A toolkit, including a patient decision aid along with its user guide and training materials, will be developed based on the findings of Phase 1 and syntheses of up-to-date evidence and appraised by the Delphi method. Additionally, a cognitive task analysis will be used for assessing the usability of the toolkit. During the evaluation stage, a prospective randomized controlled trial embedded in a mixed methods design will be used to evaluate the effectiveness and cost-effectiveness of the IP-SDM care model. The study targets to recruit 410 expectant and new mothers who screen positive for depression. They will be randomly assigned to either an intervention group or a control group in a 1:1 ratio. Participants in the intervention group will receive decision aid, decision coaching, and clinical consultation, in addition to usual services, while the control group will receive usual services. The primary outcome is the quality of decision-making process, and the secondary outcomes include SDM, mental health service utilization and costs, depressive symptoms, and health-related quality of life. In-depth ...
Keywords	Perinatal depression ; Shared decision-making ; Decision aid ; Participatory design ; Qualitative methods ; RCT ; Medicine (General) ; R5-920
Subject code	360
Language	English
Publishing date	2023-11-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Arsenic Removal via the Biomineralization of Iron-Oxidizing Bacteria Pseudarthrobacter sp. Fe7

Xia Fan / Hanxiao Zhang / Qian Peng / Yongliang Zheng / Kaixiang Shi / Xian Xia

Microorganisms, Vol 11, Iss 12, p

2023 Volume 2860

Abstract: Arsenic (As) is a highly toxic metalloid, and its widespread contamination of water is a serious threat to human health. This study explored As removal using Fe(II)-oxidizing bacteria. The strain Fe7 isolated from iron mine soil was classified as the ... ...

Abstract	Arsenic (As) is a highly toxic metalloid, and its widespread contamination of water is a serious threat to human health. This study explored As removal using Fe(II)-oxidizing bacteria. The strain Fe7 isolated from iron mine soil was classified as the genus Pseudarthrobacter based on 16S rRNA gene sequence similarities and phylogenetic analyses. The strain Fe7 was identified as a strain of Gram-positive, rod-shaped, aerobic bacteria that can oxidize Fe(II) and produce iron mineral precipitates. X-ray diffraction, X-ray photoelectron spectroscopy, and energy-dispersive X-ray spectroscopy patterns showed that the iron mineral precipitates with poor crystallinity consisted of Fe(III) and numerous biological impurities. In the co-cultivation of the strain Fe7 with arsenite (As(III)), 100% of the total Fe and 99.9% of the total As were removed after 72 h. During the co-cultivation of the strain Fe7 with arsenate (As(V)), 98.4% of the total Fe and 96.9% of the total As were removed after 72 h. Additionally, the iron precipitates produced by the strain Fe7 removed 100% of the total As after 3 h in both the As(III) and As(V) pollution systems. Furthermore, enzyme activity experiments revealed that the strain Fe7 oxidized Fe(II) by producing extracellular enzymes. When 2% ( v / v ) extracellular enzyme liquid of the strain Fe7 was added to the As(III) or As(V) pollution system, the total As removal rates were 98.6% and 99.4%, respectively, after 2 h, which increased to 100% when 5% ( v / v ) and 10% ( v / v ) extracellular enzyme liquid of the strain Fe7 were, respectively, added to the As(III) and As(V) pollution systems. Therefore, iron biomineralized using a co-culture of the strain Fe7 and As, iron precipitates produced by the strain Fe7, and the extracellular enzymes of the strain Fe7 could remove As(III) and As(V) efficiently. This study provides new insights and strategies for the efficient remediation of arsenic pollution in aquatic environments.
Keywords	arsenic removal ; Fe(II)-oxidizing bacteria ; iron mineral precipitates ; biomineralization ; Pseudarthrobacter ; Biology (General) ; QH301-705.5
Subject code	572
Language	English
Publishing date	2023-11-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
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Article: [Pathogenesis of Psoriatic Pruritus].

Zheng, Jia-Yuan / Chen, Xian-Xia / Luo, Zhi-Cheng

Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae

2022 Volume 44, Issue 3, Page(s) 529–534

Abstract: Pruritus is a common symptom of most primary skin diseases and some systemic diseases. Despite the high incidence of pruritus in patients with psoriasis,the specific pathogenesis is complex and unclear. The occurrence and development of skin pruritus in ... ...

Abstract	Pruritus is a common symptom of most primary skin diseases and some systemic diseases. Despite the high incidence of pruritus in patients with psoriasis,the specific pathogenesis is complex and unclear. The occurrence and development of skin pruritus in psoriasis patients involve the joint participation of nervous,immune,endocrine,and vascular systems.This article reviews the mediators related to the pathogenesis of pruritus in psoriasis,aiming to improve the understanding of itching symptoms and the treatment of pruritus.
MeSH term(s)	Anxiety ; Humans ; Pruritus/etiology ; Psoriasis/complications ; Skin/pathology
Language	Chinese
Publishing date	2022-03-05
Publishing country	China
Document type	Journal Article ; Review
ZDB-ID	604853-5
ISSN	1000-503X
ISSN	1000-503X
DOI	10.3881/j.issn.1000.503X.13448
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Article ; Online: Chronic heart failure serum S100B level and its short-term clinical significance

Yue-Wu Chen / Xian-Xia Liu

Journal of Hainan Medical University, Vol 25, Iss 6, Pp 28-

2019 Volume 32

Abstract: Objective: To explore the clinical significance of serum S100B in chronic heart failure (CHF). Methods: A total of 300 patients with CHF of cardiac function levels II–IV were divided into three groups, and 300 normal cases were selected as controls. ... ...

Abstract	Objective: To explore the clinical significance of serum S100B in chronic heart failure (CHF). Methods: A total of 300 patients with CHF of cardiac function levels II–IV were divided into three groups, and 300 normal cases were selected as controls. Serum S100B, B-type natriuretic peptide (BNP), LVEF(%) and LVEDD were detected and analyzed. CHF patients were followed up for 1 year. Statistical analysis was performed using SPSS and Microsoft Excel. Results: The level of S100B in CHF patients was significantly increased, and the serum level was positively correlated with BNP. The serum S100B level was positively correlated with heart failure classification, and the area under ROC curve was 0.84. The sensitivity and specificity were 81.2% and 71.5%, respectively. Patients with high serum S100B were at higher risk of major cardiovascular events. Conclusion: Serum S100B level is significantly increased in patients with chronic heart failure, and is negatively correlated with the patients’ cardiac function. It is an independent risk factor for the occurrence and development of chronic heart failure, and it has implications for the prognosis of patients with chronic heart failure.
Keywords	Medicine ; R
Subject code	610
Language	English
Publishing date	2019-03-01T00:00:00Z
Publisher	Editorial Board of Journal of Hainan Medical University
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Covalent Organic Frameworks Boost the Silver-Electrocatalyzed Reduction of CO

Yang, Xian-Xia / Du, Yi-Ran / Li, Xiao-Qiang / Duan, Guo-Yi / Chen, Yong-Mei / Xu, Bao-Hua

ACS applied materials & interfaces

2023 Volume 15, Issue 26, Page(s) 31533–31542

Abstract: The electrocatalytic reduction of ... ...

Abstract	The electrocatalytic reduction of CO
Language	English
Publishing date	2023-06-21
Publishing country	United States
Document type	Journal Article
ISSN	1944-8252
ISSN (online)	1944-8252
DOI	10.1021/acsami.3c05679
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Article ; Online: Durability of neutralization against Omicron subvariants after vaccination and breakthrough infection

Ka-Li Zhu / Xiao-Lin Jiang / Bing-Dong Zhan / Xue-Jun Wang / Xian Xia / Guo-Ping Cao / Wen-Kui Sun / Peng-Xiang Huang / Jin-Zhong Zhang / Yu-Ling Gao / Er-Hei Dai / Hui-Xia Gao / Mai-Juan Ma

Cell Reports, Vol 42, Iss 2, Pp 112075- (2023)

2023

Abstract: Summary: Booster immunizations and breakthrough infections can elicit severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron subvariant neutralizing activity. However, the durability of the neutralization response is unknown. We ... ...

Abstract	Summary: Booster immunizations and breakthrough infections can elicit severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron subvariant neutralizing activity. However, the durability of the neutralization response is unknown. We characterize the sensitivity of BA.1, BA.2, BA.2.75, BA.4/BA.5, BF.7, BQ.1.1, and XBB against neutralizing antibodies from vaccination, hybrid immunity, and breakthrough infections 4–6 months after vaccination and infection. We show that a two-dose CoronaVac or a third-dose ZF2001 booster elicits limited neutralization against Omicron subvariants 6 months after vaccination. Hybrid immunity as well as Delta, BA.1, and BA.2 breakthrough infections induce long-term persistence of the antibody response, and over 70% of sera neutralize BA.1, BA.2, BA.4/BA.5, and BF.7. However, BQ.1.1 and XBB, followed by BA.2.75, are more resistant to neutralization, with neutralizing titer reductions of ∼9- to 41-fold, ∼16- to 63-fold, and ∼4- to 25-fold, respectively. These data highlight additional vaccination in CoronaVac- or ZF2001-vaccinated individuals and provide insight into the durability of neutralization against Omicron subvariants.
Keywords	CP: Immunology ; CP: Microbiology ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2023-02-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Rab11a in the spinal cord: an essential contributor to complete Freund's adjuvant-induced inflammatory pain in mice.

Gu, Jun-Xiang / Wang, Jian / Ma, Fu-Juan / Liu, Miao-Miao / Chen, Si-Hai / Wei, Yi / Xiao, Yi-Fan / Lv, Pei-Yuan / Liu, Xin / Qu, Jian-Qiang / Yan, Xian-Xia / Chen, Tao

Molecular brain

2023 Volume 16, Issue 1, Page(s) 70

Abstract: Inflammatory pain is a commonly observed clinical symptom in a range of acute and chronic diseases. However, the mechanism of inflammatory pain is far from clear yet. Rab11a, a small molecule guanosine triphosphate enzyme, is reported to regulate ... ...

Abstract	Inflammatory pain is a commonly observed clinical symptom in a range of acute and chronic diseases. However, the mechanism of inflammatory pain is far from clear yet. Rab11a, a small molecule guanosine triphosphate enzyme, is reported to regulate orofacial inflammatory pain in our previous works. However, the mechanism of Rab11a's involvement in the regulation of inflammatory pain remains obscure. Here, we aim to elucidate the potential mechanisms through which Rab11a contributes to the development of inflammatory pain in the spinal level. It's shown that neurons, rather than glial cells, were the primary cell type expressing Rab11a in the spinal dorsal horn (SDH). After intra-plantar injection of CFA, both the number of Fos/Rab11a-immunopositive neurons and the expression of Rab11a were increased. Administration of Rab11a-shRNA into the SDH resulted in significantly analgesic effect in mice with CFA injection. Application of Rab11a-shRNA also reduced the NMDA receptor-mediated excitatory post-synaptic current (EPSC) and the spike number of neurons in lamina II of the SDH in mice with CFA injection, without affecting the presynaptic glutamate release and the postsynaptic AMPA receptor-mediated EPSC. Our results thus suggest that the enhanced expression of neuronal Rab11a may be important for the process of inflammatory pain in mice with CFA injection, which is likely mediated by Rab11a's potentiation of the competence of post-synaptic NMDAR and spiking of SDH neurons.
MeSH term(s)	Animals ; Mice ; Freund's Adjuvant ; Hyperalgesia/metabolism ; Inflammation/chemically induced ; Neurons/metabolism ; Pain/complications ; Pain/metabolism ; Posterior Horn Cells ; Receptors, N-Methyl-D-Aspartate/metabolism ; RNA, Small Interfering/metabolism ; Spinal Cord/metabolism ; Spinal Cord Dorsal Horn/metabolism
Chemical Substances	Freund's Adjuvant (9007-81-2) ; Receptors, N-Methyl-D-Aspartate ; RNA, Small Interfering ; rab11 protein (EC 3.6.1.-)
Language	English
Publishing date	2023-09-28
Publishing country	England
Document type	Journal Article
ZDB-ID	2436057-0
ISSN	1756-6606 ; 1756-6606
ISSN (online)	1756-6606
ISSN	1756-6606
DOI	10.1186/s13041-023-01057-3
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Article: Gene function and expression regulation of RuvRCAB in bacterial Cr(VI), As(III), Sb(III), and Cd(II) resistance

Wu, Shijuan / Xian Xia / Dan Wang / Zijie Zhou / Gejiao Wang

Applied microbiology and biotechnology. 2019 Mar., v. 103, no. 6

2019

Abstract: Alishewanella sp. WH16-1 is a heavy metal-resistant bacterium. Previously, a putative YebC family regulator gene, designated ruvR, was associated with Cr(VI) resistance. In this study, comprehensive analyses were performed to study the role of ruvR and ... ...

Abstract	Alishewanella sp. WH16-1 is a heavy metal-resistant bacterium. Previously, a putative YebC family regulator gene, designated ruvR, was associated with Cr(VI) resistance. In this study, comprehensive analyses were performed to study the role of ruvR and its adjunct putative DNA repairing genes, ruvCAB, in the heavy metal resistance of Alishewanella sp. WH16-1. RT-PCR analysis showed that ruvR is cotranscribed with ruvCAB. Gene mutation and complementation experiments indicated that ruvRCAB contributes to Cr(VI), As(III), Sb(III), and Cd(II) resistance in vivo. Random amplification of polymorphic DNA-PCR revealed that ruvCAB is associated with DNA repair mediated by these metals, and the presence of the metals in the cells was confirmed by elemental mapping and energy-dispersive X-ray spectrograph analysis. In addition, qRT-PCR, reporter gene assay, and in vivo and in vitro protein-DNA interaction experiments indicated that RuvR positively regulates the transcription of ruvCAB and is induced by Cr(VI). Finally, site-directed mutagenesis demonstrated that Asp103 is essential for the DNA binding ability of RuvR. The above results suggest that RuvR is involved in Cr(VI) resistance and resistance to other metals and that RuvR positively regulates the expression of ruvCAB. Based on our study and literatures, a model of RuvRCAB detailing bacterial heavy metal resistance is proposed. The RuvRCAB system plays an important role in the ability of Alishewanella sp. WH16-1 to survive in environments with heavy metals.
Keywords	Alishewanella ; DNA ; DNA repair ; antimony ; arsenic ; bacteria ; binding capacity ; cadmium ; chromium ; heavy metals ; metal tolerance ; models ; quantitative polymerase chain reaction ; regulator genes ; reporter genes ; reverse transcriptase polymerase chain reaction ; site-directed mutagenesis ; transcription (genetics)
Language	English
Dates of publication	2019-03
Size	p. 2701-2713.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	392453-1
ISSN	1432-0614 ; 0171-1741 ; 0175-7598
ISSN (online)	1432-0614
ISSN	0171-1741 ; 0175-7598
DOI	10.1007/s00253-019-09666-6
Database	NAL-Catalogue (AGRICOLA)

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Article: The essentialness of glutathione reductase GorA for biosynthesis of Se(0)-nanoparticles and GSH for CdSe quantum dot formation in Pseudomonas stutzeri TS44

Wang, Dan / Gejiao Wang / Shijuan Wu / Shixue Zheng / Xian Xia

Journal of hazardous materials. 2019 Mar. 15, v. 366

2019

Abstract: Pseudomonas stutzeri TS44 was able to aerobically reduce Se(IV) into SeNPs and transform Se(IV)/Cd(II) mixture into CdSe-QDs. The SeNPs and CdSe-QDs were systematically characterized by surface feature analyses, and the molecular mechanisms of SeNPs and ... ...

Abstract	Pseudomonas stutzeri TS44 was able to aerobically reduce Se(IV) into SeNPs and transform Se(IV)/Cd(II) mixture into CdSe-QDs. The SeNPs and CdSe-QDs were systematically characterized by surface feature analyses, and the molecular mechanisms of SeNPs and CdSe-QD formation in P. stutzeri TS44 were characterized in detail. In vivo, under 2.5 mmol/L Se(IV) exposure, GorA was essential for catalyzing of Se(IV) reduction rate decreased by 67% when the glutathione reductase gene gorA was disrupted, but it was not decreased in the glutathione synthesis rate-limiting gene gshA mutated strain compared to the wild type. The complemented strains restored the phenotypes. While under low amount of Se(IV) (0.5 mmol/L), GSH played an important role for Se(IV) reduction. In vitro, GorA catalyzed Se(IV) reduction with NADPH as the electron donor (Vmax of 3.947 ± 0.1061 μmol/min/mg protein under pH 7.0 and 28°C). In addition, CdSe-QDs were successfully synthesized by a one-step method in which Se(IV) and Cd(II) were added to bacterial culture simultaneously. GSH rather than GorA is necessary for CdSe-QD formation in vivo and in vitro. In conclusion, the results provide new findings showing that GorA functions as a selenite reductase under high amount Se(IV) and GSH is essential for bacterial CdSe-QD synthesis.
Keywords	bacterial culture ; biosynthesis ; cadmium ; genes ; glutathione ; glutathione-disulfide reductase ; NADP (coenzyme) ; pH ; phenotype ; Pseudomonas stutzeri ; quantum dots ; selenites ; selenium
Language	English
Dates of publication	2019-0315
Size	p. 301-310.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1491302-1
ISSN	1873-3336 ; 0304-3894
ISSN (online)	1873-3336
ISSN	0304-3894
DOI	10.1016/j.jhazmat.2018.11.092
Database	NAL-Catalogue (AGRICOLA)

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