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Article: CRISPR/Cas9-Mediated

Li, Yujing / Shu, Pan / Xiang, Lanting / Sheng, Jiping / Shen, Lin

2023 Volume 12, Issue 14

Abstract: Tomato fruit is highly susceptible to infection ... ...

Abstract	Tomato fruit is highly susceptible to infection by
Language	English
Publishing date	2023-07-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods12142750
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Article ; Online: CRISPR/Cas9-Mediated SlATG5 Mutagenesis Reduces the Resistance of Tomato Fruit to Botrytis cinerea

Li, Yujing / Shu, Pan / Xiang, Lanting / Sheng, Jiping / Shen, Lin

Foods. 2023 July 19, v. 12, no. 14

2023

Abstract: Tomato fruit is highly susceptible to infection by Botrytis cinerea (B. cinerea), a dominant pathogen, during storage. Recent studies have shown that autophagy is essential for plant defense against biotic and abiotic stresses. Autophagy-related gene 5 ( ... ...

Abstract	Tomato fruit is highly susceptible to infection by Botrytis cinerea (B. cinerea), a dominant pathogen, during storage. Recent studies have shown that autophagy is essential for plant defense against biotic and abiotic stresses. Autophagy-related gene 5 (ATG5) plays a key role in autophagosome completion and maturation, and is rapidly induced by B. cinerea, but the potential mechanisms of ATG5 in Solanum lycopersicum (SlATG5) in postharvest tomato fruit resistance to B. cinerea remain unclear. To elucidate the role of SlATG5 in tomato fruit resistant to B. cinerea, CRISPR/Cas9-mediated knockout of SlATG5 was used in this study. The results showed that slatg5 mutants were more vulnerable to B. cinerea and exhibited more severe disease symptoms and lower activities of disease-resistant enzymes, such as chitinase (CHI), β-1,3-glucanase (GLU), phenylalanine ammonia-lyase (PAL), and polyphenol oxidase (PPO), than the wild type (WT). Furthermore, the study observed that after inoculation with B. cinerea, the relative expression levels of genes related to salicylic acid (SA) signaling, such as SlPR1, SlEDS1, SlPAD4, and SlNPR1, were higher in slatg5 mutants than in WT. Conversely, the relative expression levels of jasmonic acid (JA) signaling-related genes SlLoxD and SlMYC2 were lower in slatg5 mutants than in WT. These findings suggested that SlATG5 positively regulated the resistance response of tomato fruit to B. cinerea by inhibiting the SA signaling pathway and activating the JA signaling pathway.
Keywords	Botrytis cinerea ; CRISPR-Cas systems ; Solanum lycopersicum ; autophagosomes ; autophagy ; catechol oxidase ; chitinase ; disease resistance ; disease severity ; disease susceptibility ; fruits ; genes ; jasmonic acid ; mutagenesis ; pathogens ; phenylalanine ammonia-lyase ; salicylic acid ; tomatoes
Language	English
Dates of publication	2023-0719
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods12142750
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Article: SlNPR1 modulates chilling stress resistance in tomato plant by alleviating oxidative damage and affecting the synthesis of ferulic acid

Shu, Pan / Li, Yujing / Xiang, Lanting / Sheng, Jiping / Shen, Lin

Scientia horticulturae. 2023 Jan. 03, v. 307

2023

Abstract: Non-expresser of pathogenesis-related genes 1 (NPR1) has been demonstrated to be a master transcription factor during resistance against pathogens, the mechanisms by which regulates chilling stress, however, remain unclear. Our results revealed that the ... ...

Abstract	Non-expresser of pathogenesis-related genes 1 (NPR1) has been demonstrated to be a master transcription factor during resistance against pathogens, the mechanisms by which regulates chilling stress, however, remain unclear. Our results revealed that the expression and protein content of SlNPR1 were dramatically induced by low temperature (4 °C). CRISPR/Cas9-mediated SlNPR1 mutagenesis aggravated the symptoms of chilling injury in tomato plant, which was accompanied with the accumulation of malonic dialdehyde (MDA), hydrogen peroxide (H₂O₂) and superoxide anion (O₂⁻), and the decrease of soluble protein content, proline content as well as antioxidant enzymes activity. In addition, slnpr1 mutants showed lower expression of SlICE1 and SlCBF1 in contrast to wild type plants (WT). PAL, C4H, C3H and COMT genes play important roles in the synthesis of ferulic acid (FA). We found that knockout of SlNPR1 reduced the expression of PAL, C4H, C3H and COMT induced by low temperature (4 °C) and inhibited the accumulation of FA content. Interestingly, FA-treated plants showed greater tolerance to chilling stress and displayed higher expression of SlICE1 and SlCBF1, exhibited lower levels of MDA and H₂O₂, but higher antioxidant enzyme (APX, POD, SOD and CAT) activity than WT. These findings reveal a new regulatory pathway that SlNPR1 enhances tolerance to chilling stress in tomato plant by alleviating oxidative damage and affecting the synthesis of FA.
Keywords	CRISPR-Cas systems ; antioxidant enzymes ; ferulic acid ; hydrogen peroxide ; mutagenesis ; proline ; protein content ; stress tolerance ; superoxide anion ; temperature ; tomatoes ; transcription factors
Language	English
Dates of publication	2023-0103
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	185557-8
ISSN	0304-4238
ISSN	0304-4238
DOI	10.1016/j.scienta.2022.111486
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Article ; Online: Two-Phase Biocatalysis in Microfluidic Droplets.

Xiang, Lanting / Kaspar, Felix / Schallmey, Anett / Constantinou, Iordania

Biosensors

2021 Volume 11, Issue 11

Abstract: This Perspective discusses the literature related to two-phase biocatalysis in microfluidic droplets. Enzymes used as catalysts in biocatalysis are generally less stable in organic media than in their native aqueous environments; however, chemical and ... ...

Abstract	This Perspective discusses the literature related to two-phase biocatalysis in microfluidic droplets. Enzymes used as catalysts in biocatalysis are generally less stable in organic media than in their native aqueous environments; however, chemical and pharmaceutical compounds are often insoluble in water. The use of aqueous/organic two-phase media provides a solution to this problem and has therefore become standard practice for multiple biotransformations. In batch, two-phase biocatalysis is limited by mass transport, a limitation that can be overcome with the use of microfluidic systems. Although, two-phase biocatalysis in laminar flow systems has been extensively studied, microfluidic droplets have been primarily used for enzyme screening. In this Perspective, we summarize the limited published work on two-phase biocatalysis in microfluidic droplets and discuss the limitations, challenges, and future perspectives of this technology.
MeSH term(s)	Biocatalysis ; Microfluidics ; Water
Chemical Substances	Water (059QF0KO0R)
Language	English
Publishing date	2021-10-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662125-3
ISSN	2079-6374 ; 2079-6374
ISSN (online)	2079-6374
ISSN	2079-6374
DOI	10.3390/bios11110407
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Article: Ethylene enhances tolerance to chilling stress in tomato fruit partially through the synergistic regulation between antioxidant enzymes and ATP synthases

Shu, Pan / Li, Yujing / Xiang, Lanting / Sheng, Jiping / Shen, Lin

Postharvest biology and technology. 2022 Nov., v. 193

2022

Abstract: Gaseous plant hormone ethylene plays a pivotal role during adaptive responses of crop growth and ripening, the mechanisms by which regulates tomato fruit response to chilling stress, however, remain limited. Our results showed that the expression of ACS2, ...

Abstract	Gaseous plant hormone ethylene plays a pivotal role during adaptive responses of crop growth and ripening, the mechanisms by which regulates tomato fruit response to chilling stress, however, remain limited. Our results showed that the expression of ACS2, ETR3, EIN2 and ERF2 and the protein content of EIL3 changed rapidly when tomato fruit exposed to 4 °C. Compared with storage at 25 °C, ethylene content and maturity of tomato fruit were inhibited at 4 °C. These results indicated that ethylene signaling might respond to chilling stress. In addition, take advantage of ethephon (ETH) or 1-Methylcyclopropene (1-MCP) treatment, we found that the severity of cold injury in tomato fruit was reduced or intensified, respectively. ETH-treated fruit displayed lower levels of ion leakage, malondialdehyde (MDA), H₂O₂ and O₂.⁻, but higher activities of antioxidant enzymes including ascorbate peroxidase (APX), peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT), as well as higher expression levels of SlICE1 and SlCBF1 than control (WT) and 1-MCP-treated fruit. Furthermore, ETH treatment attenuated chilling stress by ensuring a higher adenosine triphosphate (ATP) content, which was accompanied with higher activities of energy metabolism enzyme including H⁺-ATPase, Ca²⁺-ATPase, succinic dehydrogenase (SDH) and cytochrome C oxidase (CCO) than control and 1-MCP treatment. Correlation analysis showed that ETH treatment increased the correlation of ATP and SOD (r = 0.91), H⁺-ATP and POD (r = 0.94), H⁺-ATP and SOD (r = 0.97), while 1-MCP treatment inhibited their correlation. These findings reveal a new regulatory pathway that ethylene enhances tolerance to chilling stress in tomato fruit partially through the synergistic regulation between antioxidant enzymes and ATP synthases.
Keywords	1-methylcyclopropene ; adenosine triphosphate ; ascorbate peroxidase ; catalase ; cold injury ; cytochrome-c oxidase ; energy metabolism ; ethephon ; ethylene ; fruits ; malondialdehyde ; peroxidase ; protein content ; superoxide dismutase ; tomatoes
Language	English
Dates of publication	2022-11
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1082798-5
ISSN	1873-2356 ; 0925-5214
ISSN (online)	1873-2356
ISSN	0925-5214
DOI	10.1016/j.postharvbio.2022.112065
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Article: Ferulic acid enhances chilling tolerance in tomato fruit by up-regulating the gene expression of CBF transcriptional pathway in MAPK3-dependent manner

Shu, Pan / Li, Yujing / Li, Ziye / Xiang, Lanting / Sheng, Jiping / Shen, Lin

Postharvest biology and technology. 2022 Mar., v. 185

2022

Abstract: Ferulic acid (FA, 4-hydroxy-3-methoxycinnamic acid) is a phenolic acid derivative of cinnamic acid and plays pleiotropic roles in biotic and abiotic stress response in plants. However, the involvements of FA in chilling stress tolerance and the molecular ...

Abstract	Ferulic acid (FA, 4-hydroxy-3-methoxycinnamic acid) is a phenolic acid derivative of cinnamic acid and plays pleiotropic roles in biotic and abiotic stress response in plants. However, the involvements of FA in chilling stress tolerance and the molecular mechanisms related to mitogen-activated protein kinase (MAPK) in tomato fruit are still elusive. In this study, the effect of exogenous FA on chilling resistance of tomato fruit was investigated. In addition, CRISPR/Cas9-mediated slmapk3 mutants were used to investigate the relationship between FA and SlMAPK3 under chilling stress. The results showed that low temperature (4 ℃) induced increase of FA content and FA synthesis related genes (SlPAL5, SlC3H and SlCOMT) expression. In addition, exogenous FA reduced the severity of cold injury, up-regulated the expressions of SlMAPK3, SlCBF1 and SlICE1, and promoted the accumulation of proline and soluble protein content. However, knockout of SlMAPK3 reduced the chilling tolerance of tomato fruit and inhibited the activities of antioxidant enzymes (APX, POD, SOD and CAT) but induced the accumulation of H₂O₂. In the condition of low temperature (4 ℃), there was a high correlation between SlMAPK3 and FA synthesis related genes (SlPAL5, SlC3H, SlC4H and SlCOMT) in control fruit. However, knockout of SlMAPK3 inhibited the content of FA and the expression of those genes compared with the control, which suggested a close relationship between SlMAPK3 and FA. Specifically, the effects of FA on osmotic regulatory substance and antioxidant enzyme system as well as the gene expression of CBF (C-repeat binding transcription factor) pathway were reduced after knockout of SlMAPK3. These results unveil a function of FA in tomato fruit positively resistance to chilling stress by up-regulating the gene expression of CBF transcriptional pathway in MAPK3-dependent manner.
Keywords	CRISPR-Cas systems ; antioxidant enzymes ; cinnamic acid ; cold injury ; ferulic acid ; fruits ; gene expression ; mitogen-activated protein kinase ; proline ; protein content ; stress response ; stress tolerance ; technology ; temperature ; tomatoes ; transcription (genetics) ; transcription factors
Language	English
Dates of publication	2022-03
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1082798-5
ISSN	1873-2356 ; 0925-5214
ISSN (online)	1873-2356
ISSN	0925-5214
DOI	10.1016/j.postharvbio.2021.111775
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Arc concave sign on thin-section computed tomography：A novel predictor for invasive pulmonary adenocarcinoma in pure ground-glass nodules.

Fu, Gangze / Yu, Huibo / Liu, Jinjin / Xia, Tianyi / Xiang, Lanting / Li, Peng / Huang, Dingpin / Lin, Liaoyi / Zhuang, Yuandi / Yang, Yunjun

European journal of radiology

2021 Volume 139, Page(s) 109683

Abstract: Objective: We aimed to investigate the risk factors of invasive pulmonary adenocarcinoma, especially to report and validate the use of our newly identified arc concave sign in predicting invasiveness of pure ground-glass nodules (pGGNs).: Methods: ... ...

Abstract	Objective: We aimed to investigate the risk factors of invasive pulmonary adenocarcinoma, especially to report and validate the use of our newly identified arc concave sign in predicting invasiveness of pure ground-glass nodules (pGGNs). Methods: From January 2015 to August 2018, we retrospectively enrolled 302 patients with 306 pGGNs ≤ 20 mm pathologically confirmed (141 preinvasive lesions and 165 invasive lesions). Arc concave sign was defined as smooth and sunken part of the edge of the lesion on thin-section computed tomography (TSCT). The degree of arc concave sign was expressed by the arc chord distance to chord length ratio (AC-R); deep arc concave sign was defined as AC-R larger than the optimal cut-off value. Logistic regression analysis was used to identify the independent risk factors of invasiveness. Results: Arc concave sign was observed in 65 of 306 pGGNs (21.2 %), and deep arc concave sign (AC-R > 0.25) were more common in invasive lesions (P = 0.008). Under microscope, interlobular septal displacements were found at tumour surface. Multivariate analysis indicated that irregular shape (OR, 3.558; CI: 1.374-9.214), presence of deep arc concave sign (OR, 3.336; CI: 1.013-10.986), the largest diameter > 10.1 mm (OR, 4.607; CI: 2.584-8.212) and maximum density > -502 HU (OR, 6.301; CI: 3.562-11.148) were significant independent risk factors of invasive lesions. Conclusions: Arc concave sign on TSCT is caused by interlobular septal displacement. The degree of arc concave sign can reflect the invasiveness of pGGNs. Invasive lesions can be effectively distinguished from preinvasive lesions by the presence of deep arc concave sign, irregular shape, the largest diameter > 10.1 mm and maximum density > -502 HU in pGGNs ≤ 20 mm.
MeSH term(s)	Adenocarcinoma/diagnostic imaging ; Adenocarcinoma of Lung/diagnostic imaging ; Humans ; Lung Neoplasms/diagnostic imaging ; Neoplasm Invasiveness/diagnostic imaging ; Retrospective Studies
Language	English
Publishing date	2021-03-27
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	138815-0
ISSN	1872-7727 ; 0720-048X
ISSN (online)	1872-7727
ISSN	0720-048X
DOI	10.1016/j.ejrad.2021.109683
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Article ; Online: NAC transcription factor SlNOR-like1 plays a dual regulatory role in tomato fruit cuticle formation.

Liu, Gang-Shuai / Huang, Hua / Grierson, Donald / Gao, Ying / Ji, Xiang / Peng, Zhen-Zhen / Li, Hong-Li / Niu, Xiao-Lin / Jia, Wen / He, Jian-Lin / Xiang, Lan-Ting / Gao, Hai-Yan / Qu, Gui-Qin / Zhu, Hong-Liang / Zhu, Ben-Zhong / Luo, Yun-Bo / Fu, Da-Qi

Journal of experimental botany

2023 Volume 75, Issue 7, Page(s) 1903–1918

Abstract: The plant cuticle is an important protective barrier on the plant surface, constructed mainly by polymerized cutin matrix and a complex wax mixture. Although the pathway of plant cuticle biosynthesis has been clarified, knowledge of the transcriptional ... ...

Abstract	The plant cuticle is an important protective barrier on the plant surface, constructed mainly by polymerized cutin matrix and a complex wax mixture. Although the pathway of plant cuticle biosynthesis has been clarified, knowledge of the transcriptional regulation network underlying fruit cuticle formation remains limited. In the present work, we discovered that tomato fruits of the NAC transcription factor SlNOR-like1 knockout mutants (nor-like1) produced by CRISPR/Cas9 [clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9] displayed reduced cutin deposition and cuticle thickness, with a microcracking phenotype, while wax accumulation was promoted. Further research revealed that SlNOR-like1 promotes cutin deposition by binding to the promoters of glycerol-3-phosphate acyltransferase6 (SlGPAT6; a key gene for cutin monomer formation) and CUTIN DEFICIENT2 (SlCD2; a positive regulator of cutin production) to activate their expression. Meanwhile, SlNOR-like1 inhibits wax accumulation, acting as a transcriptional repressor by targeting wax biosynthesis, and transport-related genes 3-ketoacyl-CoA synthase1 (SlKCS1), ECERIFERUM 1-2 (SlCER1-2), SlWAX2, and glycosylphosphatidylinositol-anchored lipid transfer protein 1-like (SlLTPG1-like). In conclusion, SlNOR-like1 executes a dual regulatory effect on tomato fruit cuticle development. Our results provide a new model for the transcriptional regulation of fruit cuticle formation.
MeSH term(s)	Transcription Factors/metabolism ; Solanum lycopersicum ; Fruit/metabolism ; Gene Expression Regulation, Plant ; Phenotype ; Waxes/metabolism
Chemical Substances	Transcription Factors ; Waxes
Language	English
Publishing date	2023-10-19
Publishing country	England
Document type	Journal Article
ZDB-ID	2976-2
ISSN	1460-2431 ; 0022-0957
ISSN (online)	1460-2431
ISSN	0022-0957
DOI	10.1093/jxb/erad410
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Article: Curcumin analog A13 alleviates oxidative stress by activating Nrf2/ARE pathway and ameliorates fibrosis in the myocardium of high-fat-diet and streptozotocin-induced diabetic rats.

Xiang, Lanting / Zhang, Qiongying / Chi, Chen / Wu, Gu / Lin, Zhongmin / Li, Jianmin / Gu, Qianru / Chen, Guorong

Diabetology & metabolic syndrome

2020 Volume 12, Page(s) 1

Abstract: Background: Diabetes mellitus is an important risk factor for cardiomyopathy. Increasing oxidative stress may be one of the main factors of diabetic cardiomyopathy. A13, a newly synthesized curcumin analog, was proved to be superior to curcumin in ... ...

Abstract	Background: Diabetes mellitus is an important risk factor for cardiomyopathy. Increasing oxidative stress may be one of the main factors of diabetic cardiomyopathy. A13, a newly synthesized curcumin analog, was proved to be superior to curcumin in biological activity. However, little know about how A13 performed in diabetic models. In this study, we evaluated the ability of curcumin analog A13 to alleviate oxidative stress and ameliorate fibrosis in the myocardium, and explore the underlying mechanisms. Methods: Intraperitoneal injection of streptozotocin (30 mg/kg in 0.1 M sodium citrate buffer, pH 4.5) induced diabetes in high-fat fed rats. The rats were respectively treated with a daily dose of curcumin or A13 via intragastric intubation for 8 weeks. Myocardial tissue sections were stained with hematoxylin-eosin; oxidative stress was detected by biochemical assays; activation of the Nrf2/ARE pathway was detected by Western blot, immunohistochemical staining and RT-qPCR; myocardial fibrosis was identified by Western blot and Masson trichrome staining. Results: Treatment with curcumin analog A13 reduced the histological lesions of the myocardium in diabetic rats. Curcumin and A13 treatment decreased the malondialdehyde level and increased the activity of superoxide dismutase in the myocardium of diabetic rats. Molecular analysis and immunohistochemical staining demonstrated that dose of 20 mg/kg of A13 could activate the Nrf2/ARE pathway. Molecular analysis and Masson staining showed that curcumin analog A13 treatment significantly ameliorated fibrosis in myocardium of these diabetic rats. Conclusion: Treatment with curcumin analog A13 protects the morphology of myocardium, restores the MDA levels and SOD activity, activates the Nrf2/ARE pathway and ameliorates myocardial fibrosis in diabetic rats. It may be a useful therapeutic agent for some aspects of diabetic cardiomyopathy.
Language	English
Publishing date	2020-01-07
Publishing country	England
Document type	Journal Article
ZDB-ID	2518786-7
ISSN	1758-5996
ISSN	1758-5996
DOI	10.1186/s13098-019-0485-z
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Article: [Effects of MD2 gene silencing on high glucose-induced proliferation inhibition, apoptosis and inflammation in rat cardiomyocytes].

Lin, Zhong-Min / Chen, Guo-Rong / Zhang, Quan-Bo / Wang, Fang / Xiang, Lan-Ting / Cao, Qiong-Jie

Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology

2019 Volume 35, Issue 3, Page(s) 273–278

Abstract: Objective: To investigate the effects of myeloid differentiation-2 (MD2) gene silencing on high glucose-induced proliferation inhibition, apoptosis and inflammation in rat cardiomyocytes.: Methods: The immortalized rat cardiomyocyte cell line H9C2 ... ...

Abstract	Objective: To investigate the effects of myeloid differentiation-2 (MD2) gene silencing on high glucose-induced proliferation inhibition, apoptosis and inflammation in rat cardiomyocytes. Methods: The immortalized rat cardiomyocyte cell line H9C2 were transfected with MD2 small interfering RNA (si-MD2) and negative control for 24 h, then stimulated with high glucose (HG) for 48 h. RT-qPCR was performed to detect the mRNA levels of MD2 and inflammatory factors TNF-α, IL-1β and IL-6. MTS and flow cytometry were used to evaluate cell proliferation, cell cycle and apoptosis rate. Western blot was used to detect protein expression levels and phosphorylation levels. Results: The mRNA and protein levels of MD2 in H9C2 cells were dramatically decreased after transfected with si-MD2 (P＜0.01). After stimulation of high glucose, the mRNA levels of inflammatory factors, the cells in G0/G1 phase , the cell apoptosis rate and the protein level of cleaved Caspase-3 were significantly increased, while the cell proliferation ability was decreased (P＜0.01). MD2 gene silencing antagonized the effects of high glucose on cell proliferation, cell cycle, cell apoptosis and the mRNA levels of TNF-α, IL-1β , IL-6(P＜0.05). Western blot analysis showed that the phosphorylation levels of extracellular signal-regulated kinase(ERK1/2), P38 mitogen-activated protein kinase(P38 MAPK) and C-Jun N-terminal kinase(JNK) protein were increased significantly in H9C2 cells treated with high glucose, which could be reversed by silencing of MD2 (P＜0.01). Conclusion: This study demonstrates that MD2 gene silencing reverses high glucose-induced myocardial inflammation, apoptosis and proliferation inhibition via the mechanisms involving suppression of ERK, P38 MAPK, JNK signaling pathway.
MeSH term(s)	Animals ; Apoptosis ; Cell Proliferation ; Cells, Cultured ; Cytokines/metabolism ; Gene Silencing ; Glucose ; Inflammation ; JNK Mitogen-Activated Protein Kinases/metabolism ; Lymphocyte Antigen 96/genetics ; Myocytes, Cardiac/cytology ; Rats ; p38 Mitogen-Activated Protein Kinases/metabolism
Chemical Substances	Cytokines ; Lymphocyte Antigen 96 ; JNK Mitogen-Activated Protein Kinases (EC 2.7.11.24) ; p38 Mitogen-Activated Protein Kinases (EC 2.7.11.24) ; Glucose (IY9XDZ35W2)
Language	Chinese
Publishing date	2019-06-30
Publishing country	China
Document type	Journal Article
ISSN	1000-6834
ISSN	1000-6834
DOI	10.12047/j.cjap.5834.2019.057
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