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  1. Article ; Online: Effects of Acute and Chronic Exposure to Semicarbazide on the Sea Cucumber Apostichopus japonicus

    Xiuhui Tian / Huanjun Li / Xiuzhen Zhang / Yingjiang Xu / Huawei Zhang / Dianfeng Han / Huan Liu / Bin Wang / Yanmei Cui / Huihui Liu / Quanli Zhou / Xianghong Gong

    Frontiers in Environmental Science, Vol

    2021  Volume 9

    Abstract: The effects of acute and chronic exposure to semicarbazide were carried out on the sea cucumber Apostichopus japonicus. A half-maximal lethal concentration of 3.72 g/L of semicarbazide hydrochloride (95% confidence interval 3.43–4.02 g/L) was deduced. At ...

    Abstract The effects of acute and chronic exposure to semicarbazide were carried out on the sea cucumber Apostichopus japonicus. A half-maximal lethal concentration of 3.72 g/L of semicarbazide hydrochloride (95% confidence interval 3.43–4.02 g/L) was deduced. At 20, 4, and 2% of the half-maximal lethal concentrations, a 28-days exposure induced morphological alterations, oxidative stress, and acetylcholinesterase (AChE) activity in the respiratory tree, intestinal tract, and longitudinal muscle of A. japonicus. Exposure to 20% of the half-maximal lethal concentration resulted in lesions in the respiratory tree and disintegration in the intestinal tract. Exposure to lower concentration induced a gradual accumulation of lesions in the respiratory tree, intestinal tract, and longitudinal muscle. Levels of markers of oxidative stress and neurotransmission, including superoxide dismutase (SOD), catalase, and AChE, were increased during the initial days of exposure and then decreased. The activity of SOD, catalase, and AChE were highest in A. japonicus exposed to 4%, followed by 20 and 2% of the half-maximal lethal concentration at the same time. At the later stages of the 28-days exposure, marker levels were decreased and close to levels in the control groups. Non-targeted metabolomics indicated that significantly different metabolites were screened out, 28 in the positive ion mode and 38 in the negative ion mode, impairments in neurological function, osmotic pressure regulation, energy metabolism, and protein digestion and absorption following exposure of A. japonicus to semicarbazide. KEGG pathway enrichment showed that the exposure affected pathways related to ABC transporters, central carbon metabolism in cancer, protein digestion and absorption, aminoacyl-tRNA biosynthesis, and biosynthesis of unsaturated fatty acids.
    Keywords semicarbazide ; oxidative stress ; AChE activity ; metabolomics ; significantly different metabolites ; Environmental sciences ; GE1-350
    Subject code 333
    Language English
    Publishing date 2021-08-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: UPLC-MS/MS Method for Simultaneous Determination of Three Major Metabolites of Mequindox in Holothurian

    Huihui Liu / Chuanbo Ren / Dianfeng Han / Hui Huang / Rongjie Zou / Huawei Zhang / Yingjiang Xu / Xianghong Gong / Xiuzhen Zhang / Yanshen Li

    Journal of Analytical Methods in Chemistry, Vol

    2018  Volume 2018

    Abstract: This study developed an ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the detection of three major metabolites of mequindox, including 3-methyl-quinoxaline-2-carboxylic acid, 1-desoxymequindox, and 1,4- ... ...

    Abstract This study developed an ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the detection of three major metabolites of mequindox, including 3-methyl-quinoxaline-2-carboxylic acid, 1-desoxymequindox, and 1,4-bisdesoxymequindox (MQCA, 1-DMEQ, and BDMEQ), in holothurian. Target analytes were simplified with ultrasound-assisted acidolysis extracted without complicated enzymolysis steps. After that, each sample was centrifuged and purified by an Oasis MAX cartridge. Then, the processed samples were separated and monitored by UPLC-MS/MS. This developed method has been validated according to FDA criteria. At fortified levels of 2, 10, and 20 μg/kg, recoveries ranged from 82.5% to 93.5% with the intraday RSD less than 7.27% and interday RSD less than 11.8%. The limit of detection (LOD) of all the three metabolites ranged from 0.21 to 0.48 μg/kg, while the limit of quantification (LOQ) ranged from 0.79 to 1.59 μg/kg. On application to commercial samples, 14 of 20 samples were detected positive for the three target analytes, with positive rate at 70 percentage. The result indicated that this method was specific, sensitive, and suitable for the quantification and conformation of the three major metabolites of MEQ in holothurian.
    Keywords Analytical chemistry ; QD71-142
    Subject code 500
    Language English
    Publishing date 2018-01-01T00:00:00Z
    Publisher Hindawi Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article: Temporal and spatial distribution of semicarbazide in western Laizhou Bay

    Tian, Xiuhui / Chuanhai Xia / Quanli Zhou / Xianghong Gong / Xiukai Song / Yihao Liu / Yingjiang Xu / Zhongquan Wang

    Marine pollution bulletin. 2016 Nov. 15, v. 112, no. 1-2

    2016  

    Abstract: Semicarbazide (SEM), an industrial raw material and the marker residue of nitrofurazone as a veterinary drug, has become a new type of marine pollutant. A standard method (ultra-performance liquid chromatography–tandem mass spectrometry, UPLC–MS/MS) was ... ...

    Abstract Semicarbazide (SEM), an industrial raw material and the marker residue of nitrofurazone as a veterinary drug, has become a new type of marine pollutant. A standard method (ultra-performance liquid chromatography–tandem mass spectrometry, UPLC–MS/MS) was used to analyze SEM in seawater, sediment, and shellfish. A series of sections and stations were set up in radical distribution in western Laizhou Bay, with six voyages and 150 monitoring samples. The concentrations of SEM in seawater and shellfish were 10−11 and 10−10kg/L, respectively, and no SEM was detected in the sediment. Distribution characteristics at each state, temporal and spatial trends, multivariate analyses, and the causes were analyzed to assess the pollution level, which aimed to offer a database for drafting the national baseline values of SEM in seawater and sediment in future. The data obtained could be used for integrated watershed management of marine environment and economic activities for constructing a blue economic zone of Shandong Peninsula in China.
    Keywords databases ; liquid chromatography ; marine environment ; monitoring ; multivariate analysis ; normal values ; pollutants ; raw materials ; scanning electron microscopy ; seawater ; sediments ; shellfish ; tandem mass spectrometry ; veterinary drugs ; water pollution ; watershed management ; China
    Language English
    Dates of publication 2016-1115
    Size p. 393-398.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 2001296-2
    ISSN 1879-3363 ; 0025-326X
    ISSN (online) 1879-3363
    ISSN 0025-326X
    DOI 10.1016/j.marpolbul.2016.07.052
    Database NAL-Catalogue (AGRICOLA)

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  4. Article: Environmental status and early warning value of the pollutant Semicarbazide in Jincheng and Sishili Bays, Shandong Peninsula, China

    Tian, Xiuhui / Chuanbo Ren / Chuanhai Xia / Chunxiao Sun / Dianfeng Han / Jinglin Xue / Quanli Zhou / Xianghong Gong / Yingjiang Xu / Zhongquan Wang

    Science of the total environment. 2017 Jan. 15, v. 576

    2017  

    Abstract: A verified method for measuring Semicarbazide (SEM) in seawater, sediments, and shellfish was developed based on ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). A total of 30 stations were radially distributed in Jincheng ... ...

    Abstract A verified method for measuring Semicarbazide (SEM) in seawater, sediments, and shellfish was developed based on ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). A total of 30 stations were radially distributed in Jincheng and Sishili Bays in the Bohai and Yellow Seas, and 1025 monitoring data were collected in 41 voyages, 615 seawater samples, 320 sediment samples and 90 shellfish samples. The concentration ranged from 0.011μg/L to 0.093μg/L and 0 to 0.75μg/kg in seawater and shellfish respectively, but SEM in sediment was all below the limit of detection. Temporal and spatial distribution of SEM was investigated using multivariate analysis to estimate the degree of SEM pollution. Based on the SEM concentration in the three sample types, together with our previous findings, early warning values were deduced for SEM in seawater, and the developed method overcame shortcomings with existing technologies. The results may be helpful to draft national baseline values for SEM in seawater and sediments, and provide a scientific basis for assessing the impacts of SEM on marine ecology and human health.
    Keywords data collection ; detection limit ; human ecology ; human health ; liquid chromatography ; marine science ; monitoring ; multivariate analysis ; normal values ; pollutants ; scanning electron microscopy ; seawater ; sediments ; shellfish ; tandem mass spectrometry ; China
    Language English
    Dates of publication 2017-0115
    Size p. 868-878.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2016.10.085
    Database NAL-Catalogue (AGRICOLA)

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