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  1. Article ; Online: Research Progress of Chitosan-Based Biomimetic Materials

    Zhaoyu Zhang / Lingyu Zhang / Chengpeng Li / Xiangyu Xie / Guangfa Li / Zhang Hu / Sidong Li

    Marine Drugs, Vol 19, Iss 7, p

    2021  Volume 372

    Abstract: Chitosan is a linear polysaccharide produced by deacetylation of natural biopolymer chitin. Owing to its good biocompatibility and biodegradability, non-toxicity, and easy processing, it has been widely used in many fields. After billions of years of ... ...

    Abstract Chitosan is a linear polysaccharide produced by deacetylation of natural biopolymer chitin. Owing to its good biocompatibility and biodegradability, non-toxicity, and easy processing, it has been widely used in many fields. After billions of years of survival of the fittest, many organisms have already evolved a nearly perfect structure. This paper reviews the research status of biomimetic functional materials that use chitosan as a matrix material to mimic the biological characteristics of bivalves, biological cell matrices, desert beetles, and honeycomb structure of bees. In addition, the application of biomimetic materials in wound healing, hemostasis, drug delivery, and smart materials is briefly overviewed according to their characteristics of adhesion, hemostasis, release, and adsorption. It also discusses prospects for their application and provides a reference for further research and development.
    Keywords chitosan ; biomimetic materials ; mussels ; cell matrix ; Biology (General) ; QH301-705.5
    Subject code 540
    Language English
    Publishing date 2021-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Development of a TaqMan-based multiplex real-time PCR for simultaneous detection of four feline diarrhea-associated viruses

    Junwei Zou / Ju Yu / Yuanyuan Mu / Xiangyu Xie / Run Wang / Haiqiang Wu / Xuan Liu / Fazhi Xu / Juhua Wang / Yong Wang

    Frontiers in Veterinary Science, Vol

    2022  Volume 9

    Abstract: Since their recent discovery, the prevalence of novel feline enteric viruses, including feline bocavirus 1 (FBoV-1), feline astrovirus (FeAstV), and feline kobuvirus (FeKoV), has been reported in China. Co-infections of these viruses with feline ... ...

    Abstract Since their recent discovery, the prevalence of novel feline enteric viruses, including feline bocavirus 1 (FBoV-1), feline astrovirus (FeAstV), and feline kobuvirus (FeKoV), has been reported in China. Co-infections of these viruses with feline parvovirus (FPV) are common causes of diarrhea in cats. Viral co-infections are difficult to identify because of their non-specific clinical signs. To detect and identify these viruses, a quick and specific pathogen-testing approach is required. Here, we establish a real-time PCR (qPCR) based on multiple TaqMan probes for the simultaneous detection of FBoV-1, FeAstV, FeKoV, and FPV. Specific primers and TaqMan fluorescent probes were designed to ensure specificity. The results showed that the detection limit of single qPCR was up to 10 copies, and the detection limit of multiplex qPCR was up to 100 copies, with correlation coefficients >0.995 in all cases. Clinical sample detection revealed a 25.19% (34/135) total rate of co-infection among the viruses and a 1.48% (2/135) quadruple infection rate. Thus, this multiplex qPCR approach can serve as a quick, sensitive, and specific diagnostic tool for FBoV-1, FeAstV, FeKoV, and FPV identification, and it may be utilized for routine surveillance of these emerging and reemerging feline enteric viruses.
    Keywords TaqMan-based multiplex qPCR ; detection ; co-infection ; FPV ; FBoV-1 ; FeAstV ; Veterinary medicine ; SF600-1100
    Subject code 630
    Language English
    Publishing date 2022-11-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Tuning the upconversion photoluminescence lifetimes of NaYF4:Yb3+, Er3+ through lanthanide Gd3+ doping

    Heng Qin / Danyang Wu / Juna Sathian / Xiangyu Xie / Mary Ryan / Fang Xie

    Scientific Reports, Vol 8, Iss 1, Pp 1-

    2018  Volume 8

    Abstract: Abstract The multiplexing capacity of conventional fluorescence materials are significantly limited by spectral overlap and background interference, mainly due to their short-lived fluorescence lifetimes. Here, we adopt a novel Gd3+ doping strategy in ... ...

    Abstract Abstract The multiplexing capacity of conventional fluorescence materials are significantly limited by spectral overlap and background interference, mainly due to their short-lived fluorescence lifetimes. Here, we adopt a novel Gd3+ doping strategy in NaYF4 host materials, realized tuning of upconversion photoluminescence (UCPL) lifetimes at selective emissions. Time-correlated single-photon counting (TCSPC), was applied to measure the photoluminescence lifetimes accurately. We demonstrated the large dynamic range of lifetimes of upconversion nanoparticles with good upconversion quantum yields, mainly owing to the dominance of high efficient energy transfer upconversion mechanism. The exceptional tunable properties of upconversion materials allow great potential for them to be utilized in biotechnology and life sciences.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2018-08-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article: Research on the nitrogen cycle in rhizosphere of Kandelia obovata under ammonium and nitrate addition

    Weng, Bosen / Chongling Yan / Haoliang Lu / Jingchun Liu / Jinjin Yang / Xiangyu Xie

    Marine pollution bulletin. 2013 Nov. 15, v. 76, no. 1-2

    2013  

    Abstract: The present study investigated nitrogen process in rhizosphere of Kandelia obovata under nitrogen input. Results showed that nitrogen additions significantly increased 4 kinds of enzyme activities (Urease, Nitrate reductase, Nitrite reductase and ... ...

    Abstract The present study investigated nitrogen process in rhizosphere of Kandelia obovata under nitrogen input. Results showed that nitrogen additions significantly increased 4 kinds of enzyme activities (Urease, Nitrate reductase, Nitrite reductase and hydroxylamine reductase). The pH value increased to 7.1 under ammonium addition, but decreased to 6.9 under nitrate addition. Potential Nitrification Intensity (PNI) increased 200–1500% compared with control under ammonium addition, and Potential Denitrification Intensity (PDI) increased more than 200% under nitrate addition. Ten types of organic acids were detected from root exudates, which mainly included oxalic acid, tartaric acid, formic acid, acetic acid, and citric acid. The abundance of 5 kinds of microbial functional groups (nifH, AOA, AOB, nirS, nirK) responded differently. Total nitrogen in organs of K. obovata increased more than 200%. This indicated that nitrogen additions accelerated the transformation of nitrogen directly and stimulated the exudation of root exudates and 5 kinds of genes indirectly.
    Keywords acetic acid ; citric acid ; denitrification ; enzyme activity ; exudation ; formic acid ; genes ; hydroxylamine ; nitrate reductase ; nitrification ; nitrite reductase ; nitrogen ; nitrogen content ; nitrogen cycle ; oxalic acid ; pH ; rhizosphere ; root exudates ; tartaric acid ; urease ; water pollution
    Language English
    Dates of publication 2013-1115
    Size p. 227-240.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 2001296-2
    ISSN 1879-3363 ; 0025-326X
    ISSN (online) 1879-3363
    ISSN 0025-326X
    DOI 10.1016/j.marpolbul.2013.08.034
    Database NAL-Catalogue (AGRICOLA)

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