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  1. Article: 2023 January issue Journal of Orthopaedic Translation.

    Xiao, Guozhi

    Journal of orthopaedic translation

    2023  Volume 38, Page(s) A1–A3

    Language English
    Publishing date 2023-02-15
    Publishing country Singapore
    Document type Editorial
    ZDB-ID 2747531-1
    ISSN 2214-031X
    ISSN 2214-031X
    DOI 10.1016/j.jot.2023.01.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Primary Astrocytes Purification and Immortalization.

    Qin, Lei / Xiao, Guozhi

    Current protocols

    2023  Volume 3, Issue 12, Page(s) e964

    Abstract: Astrocytes, the most abundant cells in the central nervous system (CNS), are essential for neuronal development, network formation, and overall CNS homeostasis. Primary astrocyte culture has been successfully used as a tool to study astrocyte biology in ... ...

    Abstract Astrocytes, the most abundant cells in the central nervous system (CNS), are essential for neuronal development, network formation, and overall CNS homeostasis. Primary astrocyte culture has been successfully used as a tool to study astrocyte biology in vitro. In the present protocol, a modified immunopanning method was utilized to obtain and purify primary astrocytes from mouse cortex and spinal cord in a relatively quick and inexpensive way. Purified primary astrocytes were then immortalized through infection of lentivirus expressing the SV40 large T antigens. In addition, we provide protocols to determine the expression levels of astrocyte-specific markers and to perform functional studies measuring the ATP-induced calcium flux in the immortalized astrocytes. Following the described protocols assures that the immortalized astrocytes that one prepares mimic the cell biology of primary astrocytes in culture. Thus, the purification and immortalization protocols for primary astrocytes presented in here provide two models for the studies of astrocyte biology and may be useful for the immortalization of other types of primary cells. © 2023 Wiley Periodicals LLC. Basic Protocol 1: Primary astrocyte purification by a modified immunopanning method Support Protocol: Serum-free primary astrocyte culture Basic Protocol 2: Primary astrocyte immortalization Basic Protocol 3: Calcium transient detection in astrocytes.
    MeSH term(s) Animals ; Mice ; Astrocytes/cytology ; Primary Cell Culture/methods
    Language English
    Publishing date 2023-12-12
    Publishing country United States
    Document type Journal Article
    ISSN 2691-1299
    ISSN (online) 2691-1299
    DOI 10.1002/cpz1.964
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: One-Pot Assembly of the Highly Branched Tetradecasaccharide from

    Chen, Zhiyuan / Xiao, Guozhi

    Organic letters

    2023  Volume 25, Issue 40, Page(s) 7395–7399

    Abstract: The highly branched tetradecasaccharide repeating unit and shorter sequences of GLSWA-1 with immune-enhancing activities ... ...

    Abstract The highly branched tetradecasaccharide repeating unit and shorter sequences of GLSWA-1 with immune-enhancing activities from
    MeSH term(s) Reishi ; Polysaccharides ; Oligosaccharides ; Glycosylation
    Chemical Substances Polysaccharides ; Oligosaccharides
    Language English
    Publishing date 2023-10-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1523-7052
    ISSN (online) 1523-7052
    DOI 10.1021/acs.orglett.3c02898
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Recent chemical synthesis of plant polysaccharides.

    Wang, Xiufang / Xiao, Guozhi

    Current opinion in chemical biology

    2023  Volume 77, Page(s) 102387

    Abstract: Here, chemical syntheses of long, branched and complex glycans over 10-mer from plants are summarized, which highlights amylopectin 20-mer from starch, 17-mer from carthamus tinctorius, α-glucan 30-mer from Longan, 19-mer from psidium guajava and 11-mer ... ...

    Abstract Here, chemical syntheses of long, branched and complex glycans over 10-mer from plants are summarized, which highlights amylopectin 20-mer from starch, 17-mer from carthamus tinctorius, α-glucan 30-mer from Longan, 19-mer from psidium guajava and 11-mer from dendrobium huoshanense. The glycans assembly strategies, protecting groups utilization and glycosylation methods discussed here will inspire the efficient synthesis of diverse complex glycans with many 1,2-cis glycosidic linkages.
    MeSH term(s) Polysaccharides ; Plants ; Glycosylation ; Glycosides
    Chemical Substances Polysaccharides ; Glycosides
    Language English
    Publishing date 2023-09-14
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1439176-4
    ISSN 1879-0402 ; 1367-5931
    ISSN (online) 1879-0402
    ISSN 1367-5931
    DOI 10.1016/j.cbpa.2023.102387
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4986: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Recent Advances in Chemical Synthesis of Structural Domains of Lipopolysaccharides from the Commensal Gut-Associated Microbiota.

    Wang, Xiufang / Xiao, Guozhi

    Chembiochem : a European journal of chemical biology

    2023  Volume 24, Issue 23, Page(s) e202300552

    Abstract: Lipopolysaccharides from the commensal gut-associated microbiota are interesting biomolecules for the treatment of various inflammatory diseases. Different from pathogenic lipopolysaccharides, commensal lipopolysaccharides have distinct chemical ... ...

    Abstract Lipopolysaccharides from the commensal gut-associated microbiota are interesting biomolecules for the treatment of various inflammatory diseases. Different from pathogenic lipopolysaccharides, commensal lipopolysaccharides have distinct chemical structures and mediate beneficial homeostasis with the immune system of the host. However, the accessibility issues of homogenous and pure commensal lipopolysaccharides hampered the in-depth studies of their functions. In this concept article, we highlight the recent synthesis of lipopolysaccharides from gut-associated lymphoid-tissue-resident Alcaligenes faecalis and Bacteroides vulgatus, which hopes to inspire the more efforts devoting to these fantastic biomolecules.
    MeSH term(s) Lipopolysaccharides/chemistry ; Gastrointestinal Microbiome
    Chemical Substances Lipopolysaccharides
    Language English
    Publishing date 2023-10-04
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2020469-3
    ISSN 1439-7633 ; 1439-4227
    ISSN (online) 1439-7633
    ISSN 1439-4227
    DOI 10.1002/cbic.202300552
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Highly Stereoselective Synthesis of Branched Fructooligosaccharides ABW90-1 and ABW50-1 from

    Li, Kaifeng / Liu, Bin / Wang, Xiufang / Xiao, Guozhi

    Organic letters

    2024  Volume 26, Issue 7, Page(s) 1468–1471

    Abstract: The branched fructooligosaccharides ABW90-1 and ABW50-1 ... ...

    Abstract The branched fructooligosaccharides ABW90-1 and ABW50-1 from
    MeSH term(s) Achyranthes ; Oligosaccharides/pharmacology ; Hydrogen Bonding
    Chemical Substances fructooligosaccharide ; Oligosaccharides
    Language English
    Publishing date 2024-02-08
    Publishing country United States
    Document type Journal Article
    ISSN 1523-7052
    ISSN (online) 1523-7052
    DOI 10.1021/acs.orglett.4c00092
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Advances in osteoarthritis research in 2021 and beyond.

    Wen, Chunyi / Xiao, Guozhi

    Journal of orthopaedic translation

    2022  Volume 32, Page(s) A1–A2

    Language English
    Publishing date 2022-03-22
    Publishing country Singapore
    Document type Editorial
    ZDB-ID 2747531-1
    ISSN 2214-031X
    ISSN 2214-031X
    DOI 10.1016/j.jot.2022.02.011
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Highly Stereoselective Assembly of 1,2-

    Li, Penghua / Fan, Hanyingzi / Tan, Qiang / Xiao, Guozhi

    Organic letters

    2023  Volume 25, Issue 16, Page(s) 2788–2792

    Abstract: Highly stereoselective construction of 1,2- ...

    Abstract Highly stereoselective construction of 1,2-
    Language English
    Publishing date 2023-04-14
    Publishing country United States
    Document type Journal Article
    ISSN 1523-7052
    ISSN (online) 1523-7052
    DOI 10.1021/acs.orglett.3c00670
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: The total synthesis of rhynchosporosides

    Yang, Rui / Sun, Xingchun / Zhang, Yunqin / Xiao, Guozhi

    Organic & biomolecular chemistry

    2022  Volume 20, Issue 34, Page(s) 6755–6758

    Abstract: The efficient and collective synthesis of rhynchosporosides causing scald diseases has been achieved, which features orthogonal one-pot glycosylation on the basis of PTFAI glycosylation, Yu glycosylation, and PVB glycosylation and merging reagent ... ...

    Abstract The efficient and collective synthesis of rhynchosporosides causing scald diseases has been achieved, which features orthogonal one-pot glycosylation on the basis of PTFAI glycosylation, Yu glycosylation, and PVB glycosylation and merging reagent modulation and remote anchimeric assistance (RMRAA) α-glucosylation strategies. The issues inherent to the thioglycoside-based orthogonal one-pot glycosylation strategy, such as aglycone transfer, have been precluded by this orthogonal one-pot glycosylation strategy, which can streamline glycan chemical synthesis.
    MeSH term(s) Glycosylation ; Oligosaccharides ; Polysaccharides ; Stereoisomerism
    Chemical Substances Oligosaccharides ; Polysaccharides
    Language English
    Publishing date 2022-08-31
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2097583-1
    ISSN 1477-0539 ; 1477-0520
    ISSN (online) 1477-0539
    ISSN 1477-0520
    DOI 10.1039/d2ob01243j
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Total Synthesis of

    Ma, Yuxin / Jiang, Qiong / Wang, Xiufang / Xiao, Guozhi

    Organic letters

    2022  Volume 24, Issue 43, Page(s) 7950–7954

    Abstract: Cordyceps ... ...

    Abstract Cordyceps militaris
    MeSH term(s) Glycosylation ; Cordyceps ; Polysaccharides ; Antioxidants
    Chemical Substances Polysaccharides ; Antioxidants
    Language English
    Publishing date 2022-10-26
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1523-7052
    ISSN (online) 1523-7052
    DOI 10.1021/acs.orglett.2c03081
    Database MEDical Literature Analysis and Retrieval System OnLINE

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