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Article ; Online: Correlation between the dynamic changes of γδT cells, Th17 cells, CD4

Liu, Wei / Zhang, Jia-Hua / Gao, Lu / Xiao, Jun-Hua

2024 Volume 439, Issue 1, Page(s) 114098

Abstract: The involvement of γδT cells, Th17 cells, and ... ...

Abstract	The involvement of γδT cells, Th17 cells, and CD4
Language	English
Publishing date	2024-05-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	1493-x
ISSN	1090-2422 ; 0014-4827
ISSN (online)	1090-2422
ISSN	0014-4827
DOI	10.1016/j.yexcr.2024.114098
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Article: Benzylisoquinoline alkaloids inhibit lung fibroblast activation mainly via inhibiting TGF-β1/Smads and ERK1/2 pathway proteins.

Ren, Hui-Li / Zhang, Jia-Hua / Xiao, Jun-Hua

Heliyon

2023 Volume 9, Issue 6, Page(s) e16849

Abstract: Backgrounds: Liensinine (Lien), Neferine (Nef), Isoliensinine (Iso) and Tetrandrine (Tet), benzylisoquinoline alkaloids (BIAs), have been shown inhibitory effects on pulmonary fibrosis (PF) through anti-inflammatory, anti-oxidative activities, ... ...

Abstract	Backgrounds: Liensinine (Lien), Neferine (Nef), Isoliensinine (Iso) and Tetrandrine (Tet), benzylisoquinoline alkaloids (BIAs), have been shown inhibitory effects on pulmonary fibrosis (PF) through anti-inflammatory, anti-oxidative activities, inhibition of cytokines and NF-κB. Effects of other similar BIAs, Dauricine (Dau), Papaverine (Pap) and lotusine (Lot), on PF remain unclear. Here, we explored the effects of five bisbenzylisoquinoline (Lien, Nef, Iso, Tet and Dau) and two monobenzylisoquinoline (Pap, Lot) alkaloids on normal and PF fibroblasts. Methods: Primary normal and PF lung fibroblasts were cultured and treated with these alkaloids. Proliferation, activation, migration and apoptosis changes were detected by MTT, wound healing assay, flow cytometry. Protein level was analyzed by Western blot. Results: All BIAs inhibited proliferation of normal and PF lung fibroblasts induced by TGF-β. α-SMA protein level in normal and PF lung fibroblasts decreased after Lien, Nef, Iso, Tet and Dau treatment. Pap and Lot had no influence on α-SMA expression. Dau showed the strongest inhibitory effects on proliferation and activation among alkaloids. The migration rates of normal and PF lung fibroblasts were inhibited by Lien, Nef, Iso, and Dau. Lien, Nef, Iso and Dau significantly promoted apoptosis, while Tet had no effect on apoptosis. Pap and Lot had no influence on activation, migration and apoptosis. Dau significantly inhibited Smad3/4 and p-ERK1/2 protein overexpression induced by TGF-β1. Conclusions: Bisbenzylisoquinoline alkaloids had stronger effects on inhibiting lung fibroblasts than monobenzylisoquinoline alkaloids. Dau expressed the strongest inhibitory effects, which may be related to its inhibition of TGF-β1/Smad3/4 and p-ERK1/2 pathway proteins.
Language	English
Publishing date	2023-06-01
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2023.e16849
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Article ; Online: Ethylene Acts as a Local and Systemic Signal to Mediate UV-B-Induced Nitrate Reallocation to Arabidopsis Leaves and Roots via Regulating the ERFs-NRT1.8 Signaling Module.

Wang, Xiao-Ting / Xiao, Jun-Hua / Li, Li / Guo, Jiang-Fan / Zhang, Mei-Xiang / An, Yu-Yan / He, Jun-Min

International journal of molecular sciences

2022 Volume 23, Issue 16

Abstract: Nitrate is the preferred nitrogen source for plants and plays an important role in plant growth and development. Under various soil stresses, plants reallocate nitrate to roots to promote stress tolerance through the ethylene-ethylene response factors ( ... ...

Abstract	Nitrate is the preferred nitrogen source for plants and plays an important role in plant growth and development. Under various soil stresses, plants reallocate nitrate to roots to promote stress tolerance through the ethylene-ethylene response factors (ERFs)-nitrate transporter (NRT) signaling module. As a light signal, ultraviolet B (UV-B) also stimulates the production of ethylene. However, whether UV-B regulates nitrate reallocation in plants via ethylene remains unknown. Here, we found that UV-B-induced expression of
MeSH term(s)	Anion Transport Proteins/metabolism ; Arabidopsis/metabolism ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Ethylenes/metabolism ; Factor VIII/genetics ; Gene Expression Regulation, Plant ; Mutation ; Nitrates/metabolism ; Nitrogen Oxides/metabolism ; Plant Leaves/genetics ; Plant Leaves/metabolism ; Plant Proteins/genetics ; Plant Roots/metabolism
Chemical Substances	Anion Transport Proteins ; Arabidopsis Proteins ; Ethylenes ; Nitrates ; Nitrogen Oxides ; Plant Proteins ; Factor VIII (9001-27-8)
Language	English
Publishing date	2022-08-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms23169068
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Article ; Online: TRPC3 overexpression and intervention in airway smooth muscle of ovalbumin-induced hyperresponsiveness and remodeling.

Xu, Bo-Ming / Zhang, Jia-Hua / Wang, Jia-Ling / Xiao, Jun-Hua

Cell biology international

2018 Volume 42, Issue 8, Page(s) 1021–1029

Abstract: Transient receptor potential canonical channel 3 (TRPC3) proteins function as non-voltage-gated ... ...

Abstract	Transient receptor potential canonical channel 3 (TRPC3) proteins function as non-voltage-gated Ca
MeSH term(s)	Acetylcholine/pharmacology ; Airway Remodeling/drug effects ; Aminophylline/pharmacology ; Aminophylline/therapeutic use ; Animals ; Antibodies/immunology ; Asthma/drug therapy ; Asthma/etiology ; Asthma/veterinary ; Calcium/metabolism ; Cell Proliferation/drug effects ; Cells, Cultured ; Dexamethasone/pharmacology ; Dexamethasone/therapeutic use ; Gadolinium/pharmacology ; Hydroxyproline/metabolism ; Mice ; Muscle Contraction/drug effects ; Muscle, Smooth/cytology ; Muscle, Smooth/metabolism ; Ovalbumin/immunology ; Protein Biosynthesis/drug effects ; RNA Interference ; RNA, Small Interfering/metabolism ; TRPC Cation Channels/antagonists & inhibitors ; TRPC Cation Channels/genetics ; TRPC Cation Channels/metabolism
Chemical Substances	Antibodies ; RNA, Small Interfering ; TRPC Cation Channels ; TRPC3 cation channel ; Aminophylline (27Y3KJK423) ; Dexamethasone (7S5I7G3JQL) ; Ovalbumin (9006-59-1) ; Gadolinium (AU0V1LM3JT) ; Acetylcholine (N9YNS0M02X) ; gadolinium chloride (P7082WY76D) ; Hydroxyproline (RMB44WO89X) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2018-04-26
Publishing country	England
Document type	Journal Article
ZDB-ID	1143453-3
ISSN	1095-8355 ; 1065-6995
ISSN (online)	1095-8355
ISSN	1065-6995
DOI	10.1002/cbin.10970
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Zs.A 1451: Show issues

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Article ; Online: A novel multiplex qPCR method for assessing the comparative lengths of telomeres.

Sun, Guozhu / Cao, Hui / Bai, Yang / Wang, Jueheng / Zhou, Yuxun / Li, Kai / Xiao, Jun-Hua

Journal of clinical laboratory analysis

2021 Volume 35, Issue 9, Page(s) e23929

Abstract: Background: The comparative length of telomeres is considered to be related to diseases such as cancer, aging, and cardiovascular diseases. qPCR is currently one of the main methods for detecting telomere length. However, due to the unique sequence of ... ...

Abstract	Background: The comparative length of telomeres is considered to be related to diseases such as cancer, aging, and cardiovascular diseases. qPCR is currently one of the main methods for detecting telomere length. However, due to the unique sequence of telomeres (highly repetitive six-base sequence), it is difficult to design primers and probes to expand and detect telomere and to put internal reference gene and telomere into the same tube for detection to reduce the possible inter-pore errors and improve amplification efficiency. Besides, the stability and accuracy of the test results are greatly affected by the difference between reference genes and telomere copy number. Methods: In this study, the single-copy genes were replaced with high-copy genes (300 copies) as the internal control to reduce the copy number difference of the internal genes and telomere. In addition, a multiplex qPCR system was constructed to detect the telomeres and an internal reference gene product. We also detected the lengths of telomeres in the genomic DNA in immortalized cells (293T and Hela) from different generations of cells. Results: We detected the comparative telomere lengths of 1500 random Chinese volunteers of different ages with the multiplex qPCR method; the result shows that the comparative length of telomeres is negatively related to age. In addition, we compared our qPCR detection method with a terminal restriction fragmentation (TRF) method. Both of them were highly consistent, indicating that the qPCR method was reliable. Conclusions: In conclusion, we developed a stable, convenient, and accurate comparative telomere length detection method.
MeSH term(s)	DNA/analysis ; DNA/genetics ; HEK293 Cells ; HeLa Cells ; Humans ; Real-Time Polymerase Chain Reaction/methods ; Telomere/genetics ; Telomere Homeostasis
Chemical Substances	DNA (9007-49-2)
Language	English
Publishing date	2021-08-04
Publishing country	United States
Document type	Comparative Study ; Journal Article
ZDB-ID	645095-7
ISSN	1098-2825 ; 0887-8013
ISSN (online)	1098-2825
ISSN	0887-8013
DOI	10.1002/jcla.23929
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Zs.A 2471: Show issues

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Article ; Online: CD4

Zhang, Jia-Hua / Deng, Jia-Hui / Yao, Xia-Li / Wang, Jia-Ling / Xiao, Jun-Hua

Experimental cell research

2019 Volume 386, Issue 1, Page(s) 111700

Abstract: The immune system is felt to play an essential role in pulmonary fibrosis (PF). ... ...

Abstract	The immune system is felt to play an essential role in pulmonary fibrosis (PF). CD4
MeSH term(s)	Animals ; Bleomycin/toxicity ; Cells, Cultured ; Cytokines/genetics ; Cytokines/metabolism ; Interleukin-2 Receptor alpha Subunit/genetics ; Interleukin-2 Receptor alpha Subunit/metabolism ; Male ; Mice ; Pulmonary Fibrosis/blood ; Pulmonary Fibrosis/etiology ; Pulmonary Fibrosis/immunology ; T-Lymphocytes, Regulatory/cytology ; T-Lymphocytes, Regulatory/immunology
Chemical Substances	Cytokines ; Interleukin-2 Receptor alpha Subunit ; Bleomycin (11056-06-7)
Language	English
Publishing date	2019-10-31
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1493-x
ISSN	1090-2422 ; 0014-4827
ISSN (online)	1090-2422
ISSN	0014-4827
DOI	10.1016/j.yexcr.2019.111700
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Article: Emerging Role of MicroRNAs and Long Noncoding RNAs in Healthy and Diseased Lung.

Xiao, Jun-Hua / Hao, Qiong-Yu / Wang, Katie / Paul, Jes / Wang, Yong-Xiao

Advances in experimental medicine and biology

2017 Volume 967, Page(s) 343–359

Abstract: Pulmonary hypertension (PH) is a complex and multifactorial disease. An inability to fully unravel the molecular complexities has led to various clinical challenges in developing new therapies for this disease. Noncoding RNAs (ncRNAs) are RNA molecules ... ...

Abstract	Pulmonary hypertension (PH) is a complex and multifactorial disease. An inability to fully unravel the molecular complexities has led to various clinical challenges in developing new therapies for this disease. Noncoding RNAs (ncRNAs) are RNA molecules with limited ability of coding proteins. The amount of ncRNAs is up to 98% of the whole genome's transcripts. Many ncRNAs with a regulatory function of genes have been identified to date and found to act at various steps along the protein biosynthetic process, which includes transcription, RNA maturation, translation, and protein degradation. These discoveries are fueling a new era in understanding the pathophysiology and therapeutic pathways of PH. In this chapter, we discuss the emerging role of noncoding RNAs in PH as well as other pulmonary diseases.
Language	English
Publishing date	2017
Publishing country	United States
Document type	Journal Article
ISSN	2214-8019 ; 0065-2598
ISSN (online)	2214-8019
ISSN	0065-2598
DOI	10.1007/978-3-319-63245-2_22
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Article ; Online: Deregulated expression of Cdc6 as BCR/ABL-dependent survival factor in chronic myeloid leukemia cells.

Zhang, Jia-Hua / He, Yan-Li / Zhu, Rui / Du, Wen / Xiao, Jun-Hua

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

2017 Volume 39, Issue 6, Page(s) 1010428317713394

Abstract: Chronic myeloid leukemia is characterized by the presence of the reciprocal translocation t(9;22) and the BCR/ABL oncogene. The BCR/ABL oncogene activates multiple signaling pathways and involves the dysregulation of oncogenes during the progression of ... ...

Abstract	Chronic myeloid leukemia is characterized by the presence of the reciprocal translocation t(9;22) and the BCR/ABL oncogene. The BCR/ABL oncogene activates multiple signaling pathways and involves the dysregulation of oncogenes during the progression of chronic myeloid leukemia. The cell division cycle protein 6, an essential regulator of DNA replication, is elevated in some human cancer cells. However, the expression of cell division cycle protein 6 in chronic myeloid leukemia and the underlying regulatory mechanism remain to be elucidated. In this study, our data showed that cell division cycle protein 6 expression was significantly upregulated in primary chronic myeloid leukemia cells and the chronic myeloid leukemia cell line K562 cells, as compared to the normal bone marrow mononuclear cells. BCR/ABL kinase inhibitor STI571 or BCR/ABL small interfering RNA could significantly downregulate cell division cycle protein 6 messenger RNA expression in K562 cells. Moreover, phosphoinositide 3-kinase/AKT pathway inhibitor LY294002 and Janus kinase/signal transducer and activator of transcription pathway inhibitor AG490 could downregulate cell division cycle protein 6 expression in K562 cells, but not RAS/mitogen-activated protein kinase pathway inhibitor PD98059 had such effect. Cell division cycle protein 6 gene silencing by small interfering RNA effectively resulted in decrease of proliferation, increase of apoptosis, and arrest of cell cycle in K562 cells. These findings have demonstrated that cell division cycle protein 6 overexpression may contribute to the high proliferation and low apoptosis in chronic myeloid leukemia cells and can be regulated by BCR/ABL signal transduction through downstream phosphoinositide 3-kinase/Akt and Janus kinase/signal transducer and activator of transcription pathways, suggesting cell division cycle protein 6 as a potential therapeutic target in chronic myeloid leukemia.
MeSH term(s)	Apoptosis/drug effects ; Benzamides/administration & dosage ; Cell Cycle Proteins/biosynthesis ; Cell Cycle Proteins/genetics ; Cell Division/drug effects ; Fusion Proteins, bcr-abl/genetics ; Gene Expression Regulation, Neoplastic/drug effects ; Humans ; Imatinib Mesylate/administration & dosage ; K562 Cells ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/drug therapy ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/pathology ; Nuclear Proteins/biosynthesis ; Nuclear Proteins/genetics ; Piperazines/administration & dosage ; Pyrimidines/administration & dosage ; Signal Transduction/drug effects
Chemical Substances	BCR-ABL1 fusion protein, human ; Benzamides ; CDC6 protein, human ; Cell Cycle Proteins ; Nuclear Proteins ; Piperazines ; Pyrimidines ; Imatinib Mesylate (8A1O1M485B) ; Fusion Proteins, bcr-abl (EC 2.7.10.2)
Language	English
Publishing date	2017-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	605825-5
ISSN	1423-0380 ; 0289-5447 ; 1010-4283
ISSN (online)	1423-0380
ISSN	0289-5447 ; 1010-4283
DOI	10.1177/1010428317713394
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Zs.A 1598: Show issues

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Article ; Online: Combination of L-Arginine and L-Norvaline protects against pulmonary fibrosis progression induced by bleomycin in mice.

Gao, Lu / Zhang, Jia-Hua / Chen, Xiao-Xu / Ren, Hui-Li / Feng, Xiu-Ling / Wang, Jia-Ling / Xiao, Jun-Hua

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

2019 Volume 113, Page(s) 108768

Abstract: Pulmonary fibrosis (PF) progression may be involved with arginine (Arg) metabolism and immune balance. The present study aimed to explore the effects of L-Arginine (L-Arg) and L-Norvaline (L-Nor) on bleomycin (BLM)-induced PF in mice, meanwhile, and ... ...

Abstract	Pulmonary fibrosis (PF) progression may be involved with arginine (Arg) metabolism and immune balance. The present study aimed to explore the effects of L-Arginine (L-Arg) and L-Norvaline (L-Nor) on bleomycin (BLM)-induced PF in mice, meanwhile, and observe dynamic changes of Arg metabolism, immune balance and crosstalk between them in PF progression. Followed intratracheal instillation of BLM or saline, Kunming mice were treated orally with saline, L-Arg, L-Nor and L-Arg + L-Nor three times a day. And the mice were sacrificed on Day 3, 14 and 28 after treatment. Changes of body weight, lung index, lung hydroxyproline and histopathology were analyzed to evaluate the PF degree. Peripheral blood Arg, Citrulline (Cit), Ornithine (Orn) and Proline (Pro), lung NO, NOS and arginase were analyzed to evaluate the Arg metabolism. Peripheral blood Tregs, Th17 and γδT cells were analyzed to evaluate the immune balance. Our data showed that combination of L-Arg and L-Nor dynamically reversed the weight loss, decreased lung index and hydroxyproline, and improved lung histopathological damages induced by BLM. The combination dynamically and significantly rectified Tregs, Th17, γδT and Tregs/Th17 abnormal changes. Meanwhile, these disorders of peripheral blood Arg, Cit, Orn, Pro, Orn/Cit and Pro/Orn, and lung NO, iNOS and TNOS were also improved accordingly. These results demonstrated that combination of L-Arg and L-Nor had inhibitory effects on BLM-induced PF progression, possibly due to their corrective action on immune imbalance, Arg metabolism disorder and crosstalk abnormality in the progression of PF.
MeSH term(s)	Administration, Oral ; Animals ; Arginine/administration & dosage ; Arginine/pharmacology ; Bleomycin/toxicity ; Disease Models, Animal ; Disease Progression ; Drug Therapy, Combination ; Intraepithelial Lymphocytes/immunology ; Lung/drug effects ; Lung/pathology ; Male ; Mice ; Pulmonary Fibrosis/immunology ; Pulmonary Fibrosis/pathology ; Pulmonary Fibrosis/prevention & control ; T-Lymphocytes, Regulatory/immunology ; Th17 Cells/immunology ; Valine/administration & dosage ; Valine/analogs & derivatives ; Valine/pharmacology
Chemical Substances	Bleomycin (11056-06-7) ; Arginine (94ZLA3W45F) ; norvaline (A70UKS48FE) ; Valine (HG18B9YRS7)
Language	English
Publishing date	2019-03-17
Publishing country	France
Document type	Journal Article
ZDB-ID	392415-4
ISSN	1950-6007 ; 0753-3322 ; 0300-0893
ISSN (online)	1950-6007
ISSN	0753-3322 ; 0300-0893
DOI	10.1016/j.biopha.2019.108768
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Article: A novel multiplex fluorescent competitive PCR for copy number variation detection

Chen, Ke / Dong, Shuang-shuang / Li, Kai / Wu, Nan / Wu, Zhi-hong / Xiao, Jun-hua / Zhang, Feng / Zhou, Yu-xun

Genomics. 2019 Dec., v. 111, no. 6

2019

Abstract: The copy number variation (CNV) is an important genetic marker in cancer and other diseases. To detect CNVs of specific genetic loci, the multiplex ligation-dependent probe amplification (MLPA) is an appropriate approach, but the experimental ... ...

Abstract	The copy number variation (CNV) is an important genetic marker in cancer and other diseases. To detect CNVs of specific genetic loci, the multiplex ligation-dependent probe amplification (MLPA) is an appropriate approach, but the experimental optimization and probe synthesis are still great challenges. The multiplex competitive PCR is an alternative method for CNV detection. However, the construction of internal competitive template and establishment of a stable multiplex PCR system are the main limiting factors for this method. Here, we introduce a novel multiplex fluorescent competitive PCR (NMFC-PCR) for detecting CNVs. In this method, the blunt hairpin primers are used to rapidly establish a stable multiplex PCR system due to the reduction of non-specific amplification, and limited cycles' amplification is used to obtain the internal competitive template instead of artificial synthesis. With this method, we tested 21 clinical samples with potential LIM homeobox 1 (LHX1) or T-box 6 (TBX6) deletion. Every three segments located on the LHX1 and TBX6 were selected as the target regions, while two segments located on X-chromosome and five segments located on autosome were selected as the reference regions for detecting CNVs. The results showed that the gender information of 21 samples can be accurately inferred by the copy number ratio (CNR) of X-chromosomal reference region to autosomal reference region (X/A), and 2 samples had one copy of LHX1 and 9 samples had one copy of TBX6. To evaluate the accuracy of NMFC-PCR, 5 random samples with CNV were also detected by array-based comparative genomic hybridization (aCGH), and the results of aCGH were consistent with the NMFC-PCR results. To further assess the performance of NMFC-PCR, 60 normal samples were simultaneously tested. The results showed that the gender results were exactly the same as known information, and CNVs of LHX1 or TBX6 were not found. In conclusion, the method is a cheap, efficient, accurate, and convenient competitive PCR method for CNV detection.
Keywords	comparative genomic hybridization ; copy number variation ; fluorescence ; gender ; genetic markers ; loci ; neoplasms ; polymerase chain reaction ; X chromosome
Language	English
Dates of publication	2019-12
Size	p. 1745-1751.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	356334-0
ISSN	1089-8646 ; 0888-7543
ISSN (online)	1089-8646
ISSN	0888-7543
DOI	10.1016/j.ygeno.2018.11.029
Database	NAL-Catalogue (AGRICOLA)

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