LIVIVO - Search results -

Search results

Result 1 - 10 of total 15

Search options

Article ; Online: Effects of reverse engineering pedagogy on students’ learning performance in STEM education

Baichang Zhong / Xiaofan Liu / Xinwei Li

Heliyon, Vol 10, Iss 2, Pp e24278- (2024)

The bridge-design project as an example

2024

Abstract: In K-12 STEM education, engineering design is emphasized, as demonstrated by the bridge-design project. Due to the iterative nature of engineering design, engineering practice is frequently complicated and requires pedagogical guidance. As an emerging ... ...

Abstract	In K-12 STEM education, engineering design is emphasized, as demonstrated by the bridge-design project. Due to the iterative nature of engineering design, engineering practice is frequently complicated and requires pedagogical guidance. As an emerging pedagogy in STEM education, REP (Reverse Engineering Pedagogy) is showing, but not enough, some benefits in several cases. This paper aims to explore the effects of REP in a bridge-design course. A comparison experiment, REP versus PBL (Project-Based Learning), was conducted by randomly forming two groups of fourth-grade students from a primary school in China. Results indicated that REP was more advantageous than PBL in terms of decreasing students' cognitive load, boosting their scientific knowledge level and engineering design skills. However, REP and PBL have the same effect on the students’ learning attitude and engagement. The key findings, possible reasons, and suggestions for practice are also discussed.
Keywords	Bridge-design course ; Engineering design ; Project‐based learning ; Reverse engineering pedagogy ; STEM education ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
Subject code	370
Language	English
Publishing date	2024-01-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Molecular mechanisms of neurite regeneration and repair

Xiaofan Liu / Yuqing Zhao / Wei Zou

Cell Regeneration, Vol 12, Iss 1, Pp 1-

insights from C. elegans and Drosophila

2023 Volume 11

Abstract: Abstract The difficulties of injured and degenerated neurons to regenerate neurites and regain functions are more significant than in other body tissues, making neurodegenerative and related diseases hard to cure. Uncovering the secrets of neural ... ...

Abstract	Abstract The difficulties of injured and degenerated neurons to regenerate neurites and regain functions are more significant than in other body tissues, making neurodegenerative and related diseases hard to cure. Uncovering the secrets of neural regeneration and how this process may be inhibited after injury will provide insights into novel management and potential treatments for these diseases. Caenorhabditis elegans and Drosophila melanogaster are two of the most widely used and well-established model organisms endowed with advantages in genetic manipulation and live imaging to explore this fundamental question about neural regeneration. Here, we review the classical models and techniques, and the involvement and cooperation of subcellular structures during neurite regeneration using these two organisms. Finally, we list several important open questions that we look forward to inspiring future research.
Keywords	Neurite regeneration ; Neurite repair ; Caenorhabditis elegans ; Drosophila melanogaster ; Genetic manipulation ; Live imaging ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
Subject code	571
Language	English
Publishing date	2023-04-01T00:00:00Z
Publisher	SpringerOpen
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: A Proposed Taxonomy of Teaching Models in STEM Education

Baichang Zhong / Xiaofan Liu / Liying Xia / Wang Sun

SAGE Open, Vol

Robotics as an Example

2022 Volume 12

Abstract: Many countries and regions have reached a consensus to promote science, technology, engineering, and mathematics (STEM) education in the past decade. A body of studies have demonstrated that the design and organization of interdisciplinary teaching ... ...

Abstract	Many countries and regions have reached a consensus to promote science, technology, engineering, and mathematics (STEM) education in the past decade. A body of studies have demonstrated that the design and organization of interdisciplinary teaching activities are important for the effective implementation of STEM education. So far, however, little attention has been paid to the taxonomy of teaching models in STEM education. This paper aims to propose a taxonomy based on the dimensions of learning outcomes (i.e., product-oriented and knowledge-oriented) and teaching process (i.e., forward teaching model and reverse teaching model) in STEM education. Through the intersection of the above two dimensions, four teaching models in STEM education have emerged, including project-based learning (PBL), reverse engineering (RE), scientific inquiry (SI), and troubleshooting/debugging (T/D). In addition, four cases are introduced to explain how these four teaching models operate in STEM education. The implications of this work for future research are also discussed. It is promising that the study will be valuable to enrich the current research, and shed light on the theory and practice of STEM education.
Keywords	History of scholarship and learning. The humanities ; AZ20-999 ; Social Sciences ; H
Subject code	370
Language	English
Publishing date	2022-05-01T00:00:00Z
Publisher	SAGE Publishing
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: What should a Chinese top-level design in STEM Education look like?

Baichang Zhong / Xiaofan Liu / Zehui Zhan / Qingchao Ke / Fulai Wang

Humanities & Social Sciences Communications, Vol 9, Iss 1, Pp 1-

2022 Volume 8

Abstract: STEM education has been extensively recognized by the Chinese government and the public nationally. However, there is no consistent terminology for naming STEM education in China, which leads to confusion about the use of STEM label in practice. ... ...

Abstract	STEM education has been extensively recognized by the Chinese government and the public nationally. However, there is no consistent terminology for naming STEM education in China, which leads to confusion about the use of STEM label in practice. Meanwhile, STEM-related evaluation has not received sufficient consideration by the Chinese government except for the Ministry of Education. In addition, macro-regulation and policy support at the national level in STEM education are limited, especially for vulnerable groups, contrasting with the United States. In order to fully release the enormous potential in developing science and technology, four approaches to reforming China’s STEM-related actions are discussed: (1) China should develop a consensus terminology based on national conditions as well as international communication. (2) China’s K-12 education should move forward along with four levels of STEM education and gradually reach the corresponding thinking degrees. (3) A multi-party collaborative service mechanism should be established. (4) It is critical to establish a school culture and environment that supports the integrated implementation of STEM-related education, including targeted instruction and training for vulnerable populations. In the future, a systematic top-level design is expected to promote the development of Chinese STEM education.
Keywords	History of scholarship and learning. The humanities ; AZ20-999 ; Social Sciences ; H
Subject code	370
Language	English
Publishing date	2022-08-01T00:00:00Z
Publisher	Springer Nature
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Study on Local to Global Radiometric Balance for Remotely Sensed Imagery

Xiaofan Liu / Guoqing Zhou / Wuming Zhang / Shezhou Luo

Remote Sensing, Vol 13, Iss 2068, p

2021 Volume 2068

Abstract: Due to the difference of factors, such as lighting conditions, shooting environments, and time, there is compound brightness difference between adjacent images, which includes local brightness difference and radiometric difference. This paper proposed a ... ...

Abstract	Due to the difference of factors, such as lighting conditions, shooting environments, and time, there is compound brightness difference between adjacent images, which includes local brightness difference and radiometric difference. This paper proposed a method to eliminate the compound brightness difference of adjacent images after mosaicking, named local to global radiometric balance. It includes the brightness compensation model and brightness approach model. Firstly, the weighted average value of each row and column of image are calculated to express the brightness change; secondly, according to weighted average value, the brightness compensation model is built; thirdly, combined with the blocking method, the brightness compensation model is applied to image. Based on the value after above process, the brightness approach model is established to make the gray value of adjacent images approach to the mosaic line. In the paper, the standard deviation, MSE (mean square error) and mean value are used as the measure indices of the effect of brightness balance. The three groups of experimental results show that compared with the brightness stretch method, the histogram equalization method and the radiometric balance method, the local to global radiometric balance method not only realizes compound brightness balance, but also has better visual effects than others.
Keywords	compound brightness difference ; brightness compensation model ; brightness approach model ; blocking method ; brightness balance ; Science ; Q
Subject code	541
Language	English
Publishing date	2021-05-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Ribo-On and Ribo-Off tools using a self-cleaving ribozyme allow manipulation of endogenous gene expression in C. elegans

Jie Fang / Jie Wang / Yuzhi Wang / Xiaofan Liu / Baohui Chen / Wei Zou

Communications Biology, Vol 6, Iss 1, Pp 1-

2023 Volume 13

Abstract: Abstract Investigating gene function relies on the efficient manipulation of endogenous gene expression. Currently, a limited number of tools are available to robustly manipulate endogenous gene expression between “on” and “off” states. In this study, we ...

Abstract	Abstract Investigating gene function relies on the efficient manipulation of endogenous gene expression. Currently, a limited number of tools are available to robustly manipulate endogenous gene expression between “on” and “off” states. In this study, we insert a 63 bp coding sequence of T3H38 ribozyme into the 3’ untranslated region (UTR) of C. elegans endogenous genes using the CRISPR/Cas9 technology, which reduces the endogenous gene expression to a nearly undetectable level and generated loss-of-function phenotypes similar to that of the genetic null animals. To achieve conditional knockout, a cassette of loxP-flanked transcriptional termination signal and ribozyme is inserted into the 3’ UTR of endogenous genes, which eliminates gene expression spatially or temporally via the controllable expression of the Cre recombinase. Conditional endogenous gene turn-on can be achieved by either injecting morpholino, which blocks the ribozyme self-cleavage activity or using the Cre recombinase to remove the loxP-flanked ribozyme. Together, our results demonstrate that these ribozyme-based tools can efficiently manipulate endogenous gene expression both in space and time and expand the toolkit for studying the functions of endogenous genes.
Keywords	Biology (General) ; QH301-705.5
Subject code	570
Language	English
Publishing date	2023-08-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Close contacts and household transmission of SARS-CoV-2 in China: a content analysis based on local Heath Commissions' public disclosures.

xiaoke Xu / Xiaofan Liu / Ye Wu / Sheikh Taslim ALI

Abstract: Public disclosure from Chinese local Health Commissions show that male and mid-aged index patients are the major source of virus from outside to the households. This implies that two important actions should be taken in disease control. First considers ... ...

Abstract	Public disclosure from Chinese local Health Commissions show that male and mid-aged index patients are the major source of virus from outside to the households. This implies that two important actions should be taken in disease control. First considers the kindergarten and school closure time. Local kindergarten and primary schools should postpone their admission and regular operation, to at least not earlier than local secondary schools. Second, workplace hygiene is of particular important in the next phase of disease control. As travel restrictions within China are being gradually lifted, we suggest that workplace hygiene should be taken serious care in the next month or two.
Keywords	covid19
Publisher	medrxiv
Document type	Article ; Online
DOI	10.1101/2020.03.02.20029868
Database	COVID19

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: Spatiotemporal Evolution and Genesis of the Late Ordovician—Early Silurian Marine Euxinia in Northeastern Upper Yangtze Basin, South China

Chaoyong Wang / Zaitian Dong / Xuehai Fu / Qing Chen / Xiaofan Liu / Mengmeng Tang / Zetang Wang

Frontiers in Earth Science, Vol

2021 Volume 9

Abstract: Marine redox conditions and their dynamic changes were a major factor that controlled the formation of black shale and caused the late Ordovician marine extinction in the Upper Yangtze Basin (South China). However, the spatiotemporal variation and ... ...

Abstract	Marine redox conditions and their dynamic changes were a major factor that controlled the formation of black shale and caused the late Ordovician marine extinction in the Upper Yangtze Basin (South China). However, the spatiotemporal variation and potential controlling factors of marine redox conditions in this area remain unclear. We analyzed whole-rock geochemistry and pyrite sulfur isotopes (δ34Spy) of 47 shale samples from the Late Katian to Rhuddanian in a shelf-to-slope (Qianjiang Shaba section and Wc-1 well) region of northeastern Upper Yangtze Basin, and reconstructed water column redox conditions during the Late Ordovician–Early Silurian Transition. The geochemical characteristics of shale, including the ratio of elements, discriminant function and ternary diagram location in the study area suggest a passive continental margin sedimentary environment, wherein the terrigenous detritus is mainly derived from felsic igneous rocks in the upper crust, showing characteristics of near-source deposition. The redox indices (Fe speciation, Corg/P, UEF, and MoEF) showed that the development of anoxic water, especially euxinia, has obvious spatiotemporal heterogeneity. Under conditions of high availability of active organic carbon and limited sulfate supply, high active Fe input and strong biological irrigation in the shallow water area may effectively remove H2S produced by microbial sulfate reduction, conducive to the prevalence of ferruginous water columns. However, for this deep water area, the rapid accumulation rate of organic matter, decrease in dissolved Fe (caused by upwelling in the open sea), and seawater stratification (caused by the rising of sea level) promoted the development of a euxinic water column. This inference is supported by the covariant relationship between organic carbon accumulation rate, chemical index of alteration, Co × Mn, and δ34Spy. Our study highlights the potential control effects of sea level change, continental weathering and upwelling on the development of euxinic water columns.
Keywords	redox conditions ; δ34Spy isotope ; chemical index of alteration ; upper yangtze basin ; late ordovician—early silurian ; Science ; Q
Subject code	550
Language	English
Publishing date	2021-12-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: RNA Hydrogel Combined with MnO2 Nanoparticles as a Nano-Vaccine to Treat Triple Negative Breast Cancer

Weicai Wang / Xiaofan Liu / Lairong Ding / Hyung Jong Jin / Xuemei Li

Frontiers in Chemistry, Vol

2021 Volume 9

Abstract: Hypoxia is not only the reason of tumor metastasis but also enhances the spread of cancer cells from the original tumor site, which results in cancer recurrence. Herein, we developed a self-assembled RNA hydrogel that efficiently delivered synergistic ... ...

Abstract	Hypoxia is not only the reason of tumor metastasis but also enhances the spread of cancer cells from the original tumor site, which results in cancer recurrence. Herein, we developed a self-assembled RNA hydrogel that efficiently delivered synergistic DNA CpG and short hairpin RNA (shRNA) adjuvants, as well as MnO2 loaded-photodynamic agent chlorine e6 (MnO2@Ce6), and a chemotherapy drug doxorubicin (DOX) into MDA-MB-231cells. The RNA hydrogel consists of one tumour suppressor miRNA (miRNA-205) and one anti-metastatic miRNA (miRNA-182), both of which showed an outstanding effect in synergistically abrogating tumours. The hydrogel would be dissociated by endogenous Dicer enzyme to release loaded therapeutic molecules, and in the meantime induce decomposition of tumor endogenous H2O2 to relieve tumor hypoxia. As a result, a remarkable synergistic therapeutic effect is achieved through the combined chemo-photodynamic therapy, which simultaneously triggers a series of anti-tumor immune responses. Besides, the hydrogel as the carrier which modified aptamer to targeted MDA-MB-231 has the advantages of good biocompatibility and low cytotoxicity. This strategy could be implemented to design any other microRNA (miRNA) as the carrier, combined with other treatment methods to treat human cancer, thereby overcoming the limitations of current cancer therapies.
Keywords	RNA hydrogel ; nano-vaccine ; triple negative breast cancer ; gene therapy ; photodynamic therapy ; Chemistry ; QD1-999
Subject code	610
Language	English
Publishing date	2021-12-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: A comprehensive evaluation of full-spectrum cell-free RNAs highlights cell-free RNA fragments for early-stage hepatocellular carcinoma detectionResearch in context

Chun Ning / Peng Cai / Xiaofan Liu / Guangtao Li / Pengfei Bao / Lu Yan / Meng Ning / Kaichen Tang / Yi Luo / Hua Guo / Yunjiu Wang / Zhuoran Wang / Lu Chen / Zhi John Lu / Jianhua Yin

EBioMedicine, Vol 93, Iss , Pp 104645- (2023)

2023

Abstract: Summary: Background: Various studies have reported cell-free RNAs (cfRNAs) as noninvasive biomarkers for detecting hepatocellular carcinoma (HCC). However, they have not been independently validated, and some results are contradictory. We provided a ... ...

Abstract	Summary: Background: Various studies have reported cell-free RNAs (cfRNAs) as noninvasive biomarkers for detecting hepatocellular carcinoma (HCC). However, they have not been independently validated, and some results are contradictory. We provided a comprehensive evaluation of various types of cfRNA biomarkers and a full mining of the biomarker potential of new features of cfRNA. Methods: We first systematically reviewed reported cfRNA biomarkers and calculated dysregulated post-transcriptional events and cfRNA fragments. In 3 independent multicentre cohorts, we further selected 6 cfRNAs using RT-qPCR, built a panel called HCCMDP with AFP using machine learning, and internally and externally validated HCCMDP's performance. Findings: We identified 23 cfRNA biomarker candidates from a systematic review and analysis of 5 cfRNA-seq datasets. Notably, we defined the cfRNA domain to describe cfRNA fragments systematically. In the verification cohort (n = 183), cfRNA fragments were more likely to be verified, while circRNA and chimeric RNA candidates were neither abundant nor stable as qPCR-based biomarkers. In the algorithm development cohort (n = 287), we build and test the panel HCCMDP with 6 cfRNA markers and AFP. In the independent validation cohort (n = 171), HCCMDP can distinguish HCC patients from control groups (all: AUC = 0.925; CHB: AUC = 0.909; LC: AUC = 0.916), and performs well in distinguishing early-stage HCC patients (all: AUC = 0.936; CHB: AUC = 0.917; LC: AUC = 0.928). Interpretation: This study comprehensively evaluated full-spectrum cfRNA biomarker types for HCC detection, highlighted the cfRNA fragment as a promising biomarker type in HCC detection, and provided a panel HCCMDP. Funding: National Natural Science Foundation of China, and The National Key Basic Research Program (973 program).
Keywords	Liquid biopsy ; Liver neoplasms ; cfRNA biomarker ; Early cancer detection ; Medicine ; R ; Medicine (General) ; R5-920
Language	English
Publishing date	2023-07-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED