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  1. AU="Xiaofang Yang"
  2. AU="Merighi, Adalberto" AU="Merighi, Adalberto"

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  1. Artikel ; Online: Genome-wide characterization and expression of the TLP gene family associated with Colletotrichum gloeosporioides inoculation in Fragaria × ananassa

    Yuchao Zhang / Lixiang Miao / Xiaofang Yang / Guihua Jiang

    PeerJ, Vol 10, p e

    2022  Band 12979

    Abstract: Background Colletotrichum gloeosporioides, a soil-borne fungal pathogen, causes significant yield losses in many plants, including cultivated strawberry (Fragaria × ananassa, 2n = 8x = 56). Thaumatin-like proteins (TLPs) are a large and complex family of ...

    Abstract Background Colletotrichum gloeosporioides, a soil-borne fungal pathogen, causes significant yield losses in many plants, including cultivated strawberry (Fragaria × ananassa, 2n = 8x = 56). Thaumatin-like proteins (TLPs) are a large and complex family of proteins that play a vital role in plant host defense and other physiological processes. Methods To enhance our understanding of the antifungal activity of F. × ananassa TLPs (FaTLP), we investigated the genome-wide identification of FaTLP gene families and their expression patterns in F. × ananassa plants upon pathogen infection. Moreover, we used RNA sequencing (RNA-seq) to detect the differences in the expression patterns of TLP genes between different resistant strawberry cultivars in response to C. gloeosporioides infection. Results In total, 76 TLP genes were identified from the octoploid cultivated strawberry genome with a mean length of 1,439 bp. They were distributed on 24 F. × ananassa chromosomes. The FaTLP family was then divided into ten groups (Group I–X) according to the comparative phylogenetic results. Group VIII contained the highest number of TLP family genes. qRT-PCR analysis results indicated that FaTLP40, FaTLP41, FaTLP43, FaTLP68, and FaTLP75 were upregulated following C. gloeosporioides infection in the resistant octoploid strawberry. Conclusions The data showed some differences in TLP gene expression patterns across different resistant strawberry cultivars, as well as faster TLP defense responses to pathogenic fungi in resistant cultivars. This study will aid in the characterization of TLP gene family members found in octoploid strawberries and their potential biological functions in plants’ defenses against pathogenic fungi.
    Schlagwörter Fragaria × ananassa ; Thaumatin like-proteins ; Colletotrichum gloeosporioides ; Gene family ; Expression analysis ; Antifungal activity ; Medicine ; R ; Biology (General) ; QH301-705.5
    Thema/Rubrik (Code) 580
    Sprache Englisch
    Erscheinungsdatum 2022-03-01T00:00:00Z
    Verlag PeerJ Inc.
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: Influence of Electronic-Docking Stations on China’s Dockless Bikesharing Programs

    Xiaofang Yang / Hai Jiang

    Sustainability, Vol 12, Iss 3588, p

    Evidence from Beijing

    2020  Band 3588

    Abstract: Electronic-docking stations have been proposed and tested by some Chinese cities to address the issue of disorderly parking of dockless bikesharing programs. We study the influence of electronic-docking stations on dockless bikesharing programs, and ... ...

    Abstract Electronic-docking stations have been proposed and tested by some Chinese cities to address the issue of disorderly parking of dockless bikesharing programs. We study the influence of electronic-docking stations on dockless bikesharing programs, and measure and explain riders’ preferences toward dockless bikesharing and electronic-docking stations. These research questions are addressed by a mixed logit model and regression models based on stated preference survey data collected in Beijing. We obtain some interesting empirical results which offer some guidance for governments and operators to facilitate the sustainable development of dockless bikesharing programs. For instance, we find that riders are sensitive to the density for electronic-docking stations, but somewhat indifferent to the rental fee of 1–2 yuan for each ride; the choice probability of riding a dockless shared bike drops by about 8%–9% when access or egress time increases by 5 min; the fraction of riders interested in dockless bikesharing programs is close to 100%, while launching electronic-docking stations reduces this number to 88%; riders are willing to pay about 1.0–1.1 yuan to save access or egress time by 5 min; riders who live or work away from public transit stations or who often engage in physical exercises are interested in dockless bikesharing programs; and riders indifferent to the introduction of electronic-docking stations mainly include those who are above 26 years old and those with monthly income less than or equal to 8000 yuan.
    Schlagwörter dockless bikesharing programs ; electronic-docking stations ; riders’ preferences ; Environmental effects of industries and plants ; TD194-195 ; Renewable energy sources ; TJ807-830 ; Environmental sciences ; GE1-350
    Thema/Rubrik (Code) 551
    Sprache Englisch
    Erscheinungsdatum 2020-04-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  3. Artikel ; Online: Parallel MR image reconstruction based on triple cycle optimization

    Jinhua Sheng / Jie Yin / Luyun Wang / Xiaofang Yang / Pu Huang

    Scientific Reports, Vol 12, Iss 1, Pp 1-

    2022  Band 12

    Abstract: Abstract The self-calibration parallel imaging (SC-SENSE) method reconstructs the image by estimating the coil sensitivity matrix. In order to obtain the sensitivity matrix, it is necessary to take a small amount of automatic calibration signal lines ( ... ...

    Abstract Abstract The self-calibration parallel imaging (SC-SENSE) method reconstructs the image by estimating the coil sensitivity matrix. In order to obtain the sensitivity matrix, it is necessary to take a small amount of automatic calibration signal lines (ACSL) in the center of k-space. This method uses the data of the central region to obtain the sensitivity matrix, and then the reconstructed image is obtained. This paper proposed the triple cycle optimization (TCO) method to continuously optimize reconstructed images. The proposed TCO method takes the sensitivity matrix obtained by ACSL and substituted the reconstructed image as the initial data generation into the loop, and estimates the k-space data repeatedly. A new sensitivity matrix is obtained by using k-space data and the reconstructed image, and a stable triple cycle is obtained. In the cycle, all data are optimized to a certain extent, including the reconstructed image. Experimental results show that under the same sampling density, images reconstructed by using the triple cycle optimization method have lower noise and artifacts than those of the traditional method. When combined with the variable density sampling method, the effect is remarkable with a much low sampling rate.
    Schlagwörter Medicine ; R ; Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2022-05-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  4. Artikel ; Online: Design and Properties of Natural Rosin-Based Phosphoester Functional Surfactants

    Maogong Wang / Xiaofang Yang / Bing Han / Shifeng Zhang / Chunrui Han / Changlei Xia

    Molecules, Vol 28, Iss 3091, p

    2023  Band 3091

    Abstract: As an important forestry biomass resource, rosin has a wide range of applications in medicine, adhesives, surfactants and other fields. Using natural dehydroabietic acid as a raw material, dehydroabietic acid-based phosphorus monoester (DPM) and diester ( ...

    Abstract As an important forestry biomass resource, rosin has a wide range of applications in medicine, adhesives, surfactants and other fields. Using natural dehydroabietic acid as a raw material, dehydroabietic acid-based phosphorus monoester (DPM) and diester (DPD) surfactants were designed and synthesized. The chemical structures and self-assembly properties were characterized by FT-IR, NMR and TEM, and the effects of pH on critical micelle concentration, γ CMC , emulsifying properties, foam properties and micelle morphology were studied. The results showed that the CMC, γ CMC value and aggregate morphology had certain pH responsiveness. The γ CMC value under acidic conditions was smaller than γ CMC under alkaline conditions, and the foaming performance and foam stability under acidic conditions were better than those under alkaline conditions. TEM micelle morphology studies have shown that DPM and DPD surfactants can self-assemble into rod-shaped and spherical micelle morphologies with a pH change in an aqueous solution. At the same pH, the foaming and emulsification properties of DPD were better than those of DPM. The best foaming and emulsification ability of DPD were 11.8 mL and 175 s, respectively. At the same time, the foaming ability of DPD is also affected by pH. DPD has excellent foaming properties in acidic conditions, but these disappeared in neutral conditions.
    Schlagwörter dehydroabietic acid ; phosphate ; surfactant ; natural rosin ; Organic chemistry ; QD241-441
    Thema/Rubrik (Code) 540
    Sprache Englisch
    Erscheinungsdatum 2023-03-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  5. Artikel ; Online: Mechanistic Insights into Stereospecific Antifungal Activity of Chiral Fungicide Prothioconazole against Fusarium oxysporum F. sp. cubense

    Xiaofang Yang / Ronggao Gong / Yuanqi Chu / Siwen Liu / Dandan Xiang / Chunyu Li

    International Journal of Molecular Sciences, Vol 23, Iss 2352, p

    2022  Band 2352

    Abstract: As a typical triazole fungicide, prothioconazole (Pro) has been used extensively due to its broad spectrum and high efficiency. However, as a racemic mixture of two enantiomers ( R -Pro and S -Pro), the enantiomer-specific outcomes on the bioactivity ... ...

    Abstract As a typical triazole fungicide, prothioconazole (Pro) has been used extensively due to its broad spectrum and high efficiency. However, as a racemic mixture of two enantiomers ( R -Pro and S -Pro), the enantiomer-specific outcomes on the bioactivity have not been fully elucidated. Here, we investigate how chirality affects the activity and mechanism of action of Pro enantiomers on Fusarium oxysporum f. sp. cubense tropical race 4 ( Foc TR4), the notorious virulent strain causing Fusarium wilt of banana (FWB). The Pro enantiomers were evaluated in vivo and in vitro with the aid of three bioassay methods for their fungicidal activities against TR4 and the results suggested that the fungicidal activities of Pro enantiomers are stereoselective in a dose-dependent manner with R -Pro making a major contribution to the treatment outcomes. We found that R -Pro led to more severe morphological changes and impairment in membrane integrity than S -Pro. R -Pro also led to the increase of more MDA contents and the reduction of more SOD and CAT activities compared with the control and S -Pro groups. Furthermore, the expression of Cytochrome P450 14α-sterol demethylases (CYP51), the target for triazole fungicides, was significantly increased upon treatment with R -Pro rather than S -Pro, at both transcriptional and translational levels; so were the activities of the Cytochrome P450 enzymes. In addition, surface plasmon resonance (SPR) and molecular docking illuminated the stereoselective interactions between the Pro enantiomers and CYP51 of TR4 at the target site, and R -Pro showed a better binding affinity with CYP51 than S -Pro. These results suggested an enantioselective mechanism of Pro against TR4, which may rely on the enantioselective damages to the fungal cell membrane and the enantiospecific CYP51 binding affinity. Taken together, our study shed some light on the mechanisms underlying the differential activities of the Pro enantiomers against TR4 and demonstrated that Pro can be used as a potential candidate in the ...
    Schlagwörter prothioconazole ; enantiomer ; Fusarium wilt of banana ; Foc TR4 ; stereoselective ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Thema/Rubrik (Code) 500 ; 571
    Sprache Englisch
    Erscheinungsdatum 2022-02-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  6. Artikel ; Online: Effect of the WeChat Platform Health Management and Refined Continuous Nursing Model on Life Quality of Patients with Acute Myocardial Infarction after PCI

    Meifang Xu / Xiaofang Yang / Lin Liu / Yunlang Dai / Mingzhu Xu / Lin Shi

    Journal of Healthcare Engineering, Vol

    2021  Band 2021

    Abstract: The purpose was to explore the effect of the WeChat platform health management and refined continuous nursing model on life quality of patients with acute myocardial infarction (AMI) after PCI. 100 AMI patients treated in the cardiovascular medicine of ... ...

    Abstract The purpose was to explore the effect of the WeChat platform health management and refined continuous nursing model on life quality of patients with acute myocardial infarction (AMI) after PCI. 100 AMI patients treated in the cardiovascular medicine of the First Affiliated Hospital of Soochow University from June 2018 to June 2019 were selected as the study subjects and randomly divided into research group and reference group, with 50 cases in each group. The reference group received routine nursing after PCI, while the research group received WeChat platform health management and continuous refined nursing. There were no significant differences in sex ratio, age, BMI, complications, education level, and residence between the two groups of patients (P>0.05). The MPR values of patients in the two groups after intervention were significantly higher than those before intervention (P<0.05), and the MPR value in the research group after intervention was significantly higher than that in the reference group (P<0.05). The SF-36 scores of patients in the two groups after intervention were significantly higher than those before intervention (P<0.001), and the SF-36 score in the research group after intervention was higher than that in the reference group (P<0.001). The emotional, physical, and economic dimensions of patients in the research group after intervention were significantly lower than those in the reference group (P<0.001). The HAMA and HAMD scores of patients in the research group after intervention were significantly lower than those in the reference group (P<0.001). The nursing satisfaction score of patients in the research group was significantly higher than that in the reference group (P<0.001). The total incidence of complications of patients in the research group after intervention was significantly lower than that in the reference group (P<0.05). The WeChat platform health management and refined continuous nursing model can effectively improve the medication compliance of ...
    Schlagwörter Medicine (General) ; R5-920 ; Medical technology ; R855-855.5
    Thema/Rubrik (Code) 796
    Sprache Englisch
    Erscheinungsdatum 2021-01-01T00:00:00Z
    Verlag Hindawi Limited
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  7. Artikel ; Online: Refinery wastewater treatment via a multistage enhanced biochemical process

    Chunhua Wang / Zijian Chen / Yuanhua Li / Kejun Feng / Zhongli Peng / Yongjuan Zhu / Xiaofang Yang

    Scientific Reports, Vol 11, Iss 1, Pp 1-

    2021  Band 11

    Abstract: Abstract Petroleum refinery wastewater (PRWW) that contains recalcitrant components as the major portion of constituents is difficult to treat by conventional biological processes. An effective and economical biological treatment process was established ... ...

    Abstract Abstract Petroleum refinery wastewater (PRWW) that contains recalcitrant components as the major portion of constituents is difficult to treat by conventional biological processes. An effective and economical biological treatment process was established to treat industrial PRWW with an influent COD of over 2500 mg L−1 in this research. This process is mainly composed of internal circulation biological aerated filter (ICBAF), hydrolysis acidfication (HA), two anaerobic–aerobic (A/O) units, a membrane biological reactor (MBR), and ozone-activated carbon (O3-AC) units. The results showed that, overall, this system removed over 94% of the COD, BOD5, ammonia nitrogen (NH4 +-N) and phosphorus in the influent, with the ICBAF unit accounting for 54.6% of COD removal and 83.6% of BOD5 removal, and the two A/O units accounting for 33.3% of COD removal and 9.4% of BOD5 removal. The degradation processes of eight organic pollutants and their removal via treatment were also analyzed. Furthermore, 26 bacteria were identified in this system, with Proteobacteria and Acidobacteria being the most dominant. Ultimately, the treatment process exhibited good performance in degrading complex organic pollutants in the PRWW.
    Schlagwörter Medicine ; R ; Science ; Q
    Thema/Rubrik (Code) 660
    Sprache Englisch
    Erscheinungsdatum 2021-05-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  8. Artikel ; Online: Comparative efficacy of different types of acupuncture as adjuvant therapy on carotid atherosclerosis

    Jiao Liu / Ning Zhang / Zhihong Yang / Qian Mo / Xianming Wu / Xiaolou Huang / Shuangmei Xu / Xiaofang Yang

    BMJ Open, Vol 12, Iss

    a protocol for systematic review and network meta-analysis

    2022  Band 4

    Schlagwörter Medicine ; R
    Sprache Englisch
    Erscheinungsdatum 2022-04-01T00:00:00Z
    Verlag BMJ Publishing Group
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  9. Artikel ; Online: Trends in Overweight and Obesity Among Children and Adolescents in China from 1991 to 2015

    Yaru Guo / Xiaojian Yin / Huipan Wu / Xiaojiang Chai / Xiaofang Yang

    International Journal of Environmental Research and Public Health, Vol 16, Iss 23, p

    A Meta-Analysis

    2019  Band 4656

    Abstract: This meta-analysis of overweight and obesity (ow/ob) among children and adolescents in China from 1991 to 2015 provides a reference for promoting the healthy development of children and adolescents. The studies were retrieved from the China National ... ...

    Abstract This meta-analysis of overweight and obesity (ow/ob) among children and adolescents in China from 1991 to 2015 provides a reference for promoting the healthy development of children and adolescents. The studies were retrieved from the China National Knowledge Infrastructure, Wanfang, and PubMed databases for the period from January 1991 to January 2018. The data were combined and analyzed, and the combined effect magnitude odds ratio and the 95% confidence interval were calculated. Publication bias was determined using Stata/SE12.0. We found that (1) the prevalence of ow/ob increased from 5.0% and 1.7% in 1991−1995 to 11.7% and 6.8% in 2011−2015, respectively, and the overweight rate was the greatest in 2006−2010; (2) from 1991 to 2015, the prevalence of ow/ob was greater in urban areas than in rural areas; (3) compared with girls, boys were more likely to be ow/ob; and (4) the prevalence rates of ow and ob were greater in infancy than in other growth stages, with values of 11.7% and 7.0%, respectively. The prevalence of ow/ob among Chinese children and adolescents showed significant differences based on region, sex, and age. An overall upward trend was observed that decreased slightly from 2011 to 2015.
    Schlagwörter chinese children and adolescents ; overweight ; obesity ; prevalence ; Medicine ; R
    Thema/Rubrik (Code) 950
    Sprache Englisch
    Erscheinungsdatum 2019-11-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  10. Artikel ; Online: Physical fitness reference standards for Chinese children and adolescents

    Feng Zhang / Cunjian Bi / Xiaojian Yin / Qi Chen / Yuqiang Li / Yuan Liu / Ting Zhang / Ming Li / Yi Sun / Xiaofang Yang

    Scientific Reports, Vol 11, Iss 1, Pp 1-

    2021  Band 12

    Abstract: Abstract To develop age- and sex-specific physical fitness reference standards and express the age- and sex-related differences using standardized effect sizes for Chinese children and adolescents. A total of 85,535 children and adolescents (48.7% girls) ...

    Abstract Abstract To develop age- and sex-specific physical fitness reference standards and express the age- and sex-related differences using standardized effect sizes for Chinese children and adolescents. A total of 85,535 children and adolescents (48.7% girls) aged 7–18 years were recruited from six geographical divisions of China using a stratified randomized cluster sampling method. Seven physical fitness items including grip strength, standing long jump, 30-s sit-ups, sit and reach, 50-m dash, 20-s repeated straddling, and 20-m SRT were measured following a standardized procedure. Percentile curves for each physical fitness test were calculated using the LMS. Age- and sex-related differences were expressed as standardized effect sizes. We observed that the performance improved with age along with the analyzed percentiles in all tests. Boys had higher values compared to girls in all the physical fitness items except for sit and reach test, where girls showed better performance in all analyzed percentiles. Also, the sex differences increased with ages except sit and reach. There is a need for a differentiated approach in the physical education class in terms of adjustment of physical activity based on sex, level of fitness abilities in China.
    Schlagwörter Medicine ; R ; Science ; Q
    Thema/Rubrik (Code) 796
    Sprache Englisch
    Erscheinungsdatum 2021-03-01T00:00:00Z
    Verlag Nature Portfolio
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    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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