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  1. Artikel ; Online: Study on Anti-Constipation Effects of Hemerocallis citrina Baroni through a Novel Strategy of Network Pharmacology Screening

    Yuxuan Liang / Xiaoyi Wei / Rui Ren / Xuebin Zhang / Xiyao Tang / Jinglan Yang / Xiaoqun Wei / Riming Huang / Gary Hardiman / Yuanming Sun / Hong Wang

    International Journal of Molecular Sciences, Vol 24, Iss 4844, p

    2023  Band 4844

    Abstract: Daylily ( Hemerocallis citrina Baroni) is an edible plant widely distributed worldwide, especially in Asia. It has traditionally been considered a potential anti-constipation vegetable. This study aimed to investigate the anti-constipation effects of ... ...

    Abstract Daylily ( Hemerocallis citrina Baroni) is an edible plant widely distributed worldwide, especially in Asia. It has traditionally been considered a potential anti-constipation vegetable. This study aimed to investigate the anti-constipation effects of daylily from the perspective of gastro-intestinal transit, defecation parameters, short-chain organic acids, gut microbiome, transcriptomes and network pharmacology. The results show that dried daylily (DHC) intake accelerated the defecation frequency of mice, while it did not significantly alter the levels of short-chain organic acids in the cecum. The 16S rRNA sequencing showed that DHC elevated the abundance of Akkermansia , Bifidobacterium and Flavonifractor , while it reduced the level of pathogens (such as Helicobacter and Vibrio ). Furthermore, a transcriptomics analysis revealed 736 differentially expressed genes (DEGs) after DHC treatment, which are mainly enriched in the olfactory transduction pathway. The integration of transcriptomes and network pharmacology revealed seven overlapping targets ( Alb , Drd2 , Igf2 , Pon1 , Tshr , Mc2r and Nalcn ). A qPCR analysis further showed that DHC reduced the expression of Alb, Pon1 and Cnr1 in the colon of constipated mice. Our findings provide a novel insight into the anti-constipation effects of DHC.
    Schlagwörter daylily ; constipation ; 16S rRNA ; transcriptomes ; network pharmacology ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Sprache Englisch
    Erscheinungsdatum 2023-03-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: PVAm Nanofibers Fabricated by Rotary Jet Wet Spinning and Applied to Bisphenol A Recognition

    Yingzhu Wu / Chen Li / Fei Fan / Jiahao Liang / Zihang Yang / Xiaoqun Wei / Shuixia Chen

    ACS Omega, Vol 4, Iss 25, Pp 21361-

    2019  Band 21369

    Schlagwörter Chemistry ; QD1-999
    Sprache Englisch
    Erscheinungsdatum 2019-12-01T00:00:00Z
    Verlag American Chemical Society
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  3. Artikel: Determination of Vanillin and Ethyl-Vanillin in Milk Powder by Headspace Solid-Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry

    Wang, Zhiyuan / Baojun Xu / Bo Ding / Cheng Huang / Guangfeng Zeng / Xiaoqun Wei

    Food analytical methods. 2016 Dec., v. 9, no. 12

    2016  

    Abstract: A method for determination of vanillin and ethyl-vanillin in milk powder by auto headspace solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS) was established. Vanillins in sample were extracted with SPME through ... ...

    Abstract A method for determination of vanillin and ethyl-vanillin in milk powder by auto headspace solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS) was established. Vanillins in sample were extracted with SPME through a fused-silica fiber coated with divinylbenzene/polydimethylsiloxane. The analytes were desorbed by GC at 260 °C for 5 min and separated on a DB-5MS capillary column and detected by MS with EI source by selected ion monitoring mode (EI-SIM). As a result, the limit of detection of vanillin and ethyl-vanillin was 0.1 and 0.05 mg/kg. The spiked recovery was between 90.0 and 100 %, and the RSD ranged from 1.9 to 5.1 % (n = 6). This method eliminated the interferences from complicated matrix effectively. The method was successfully applied for quick determination of vanillin and ethyl-vanillin content in milk powder samples.
    Schlagwörter detection limit ; dried milk ; gas chromatography-mass spectrometry ; headspace analysis ; monitoring ; solid phase microextraction ; vanillin
    Sprache Englisch
    Erscheinungsverlauf 2016-12
    Umfang p. 3360-3366.
    Erscheinungsort Springer US
    Dokumenttyp Artikel
    ZDB-ID 2429656-9
    ISSN 1936-976X ; 1936-9751
    ISSN (online) 1936-976X
    ISSN 1936-9751
    DOI 10.1007/s12161-016-0520-8
    Datenquelle NAL Katalog (AGRICOLA)

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  4. Artikel: Broad-Specificity Immunoassay for Simultaneous Detection of Ochratoxins A, B, and C in Millet and Maize

    Zhang, Yaqiong / Lanteng Wang / Xing Shen / Xiaoqun Wei / Xinan Huang / Yingju Liu / Xiulan Sun / Zhanhui Wang / Yuanming Sun / Sergei A. Eremin / Hongato Lei / Zhenlin Xu

    Journal of agricultural and food chemistry. 2017 June 14, v. 65, no. 23

    2017  

    Abstract: Ochratoxins A, B, and C (OTA, OTB, and OTC) can be found in cereals and feeds; the simultaneous detection of these ochratoxins holds a great need in food safety. In this study, four antibodies raised from two ochrotoxin haptens and two coating antigens ... ...

    Abstract Ochratoxins A, B, and C (OTA, OTB, and OTC) can be found in cereals and feeds; the simultaneous detection of these ochratoxins holds a great need in food safety. In this study, four antibodies raised from two ochrotoxin haptens and two coating antigens were compared, and then a sensitive and broad-specificity enzyme-linked immunosorbent assay (ELISA) was established for the simultaneous determination of three ochratoxins, where the detection limits were 0.005, 0.001, and 0.001 ng/mL for OTA, OTB, and OTC, respectively, and recoveries of three ochratoxins were between 84.3% and 111.7%. This developed method had been successfully applied to detect ochratoxins in both millet and maize. Molecular modeling revealed that the broad-specificity was related with the chlorine electronegativity on OTA and OTC and the potential of the acetyl ester group on OTC. The proposed ELISA can be used for simultaneous detection of three ochratoxins.
    Schlagwörter antibodies ; chlorine ; corn ; enzyme-linked immunosorbent assay ; feeds ; food safety ; haptens ; millets ; molecular models ; ochratoxins
    Sprache Englisch
    Erscheinungsverlauf 2017-0614
    Umfang p. 4830-4838.
    Erscheinungsort American Chemical Society, Books and Journals Division
    Dokumenttyp Artikel
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.7b00770
    Datenquelle NAL Katalog (AGRICOLA)

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