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  1. Article ; Online: Tea-YOLOv8s: A Tea Bud Detection Model Based on Deep Learning and Computer Vision.

    Xie, Shuang / Sun, Hongwei

    Sensors (Basel, Switzerland)

    2023  Volume 23, Issue 14

    Abstract: Tea bud target detection is essential for mechanized selective harvesting. To address the challenges of low detection precision caused by the complex backgrounds of tea leaves, this paper introduces a novel model called Tea-YOLOv8s. First, multiple data ... ...

    Abstract Tea bud target detection is essential for mechanized selective harvesting. To address the challenges of low detection precision caused by the complex backgrounds of tea leaves, this paper introduces a novel model called Tea-YOLOv8s. First, multiple data augmentation techniques are employed to increase the amount of information in the images and improve their quality. Then, the Tea-YOLOv8s model combines deformable convolutions, attention mechanisms, and improved spatial pyramid pooling, thereby enhancing the model's ability to learn complex object invariance, reducing interference from irrelevant factors, and enabling multi-feature fusion, resulting in improved detection precision. Finally, the improved YOLOv8 model is compared with other models to validate the effectiveness of the proposed improvements. The research results demonstrate that the Tea-YOLOv8s model achieves a mean average precision of 88.27% and an inference time of 37.1 ms, with an increase in the parameters and calculation amount by 15.4 M and 17.5 G, respectively. In conclusion, although the proposed approach increases the model's parameters and calculation amount, it significantly improves various aspects compared to mainstream YOLO detection models and has the potential to be applied to tea buds picked by mechanization equipment.
    Language English
    Publishing date 2023-07-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2052857-7
    ISSN 1424-8220 ; 1424-8220
    ISSN (online) 1424-8220
    ISSN 1424-8220
    DOI 10.3390/s23146576
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Regional anesthesia might reduce recurrence and metastasis rates in adult patients with cancers after surgery: a meta-analysis.

    Xie, Shuang / Li, Liang / Meng, Fanqing / Wang, Huanliang

    BMC anesthesiology

    2024  Volume 24, Issue 1, Page(s) 19

    Abstract: Background: The influence of anesthesia techniques on cancer recurrence and metastasis following oncological surgery is a topic of growing interest. This meta-analysis investigates the potential effects of regional anesthesia (RA), either independently ... ...

    Abstract Background: The influence of anesthesia techniques on cancer recurrence and metastasis following oncological surgery is a topic of growing interest. This meta-analysis investigates the potential effects of regional anesthesia (RA), either independently or combined with general anesthesia (GA), on these outcomes.
    Methods: We performed an extensive search across PubMed, Embase, and the Cochrane Library databases. The primary outcome was cancer recurrence, while the secondary outcomes were local recurrence and distant metastasis. Pooled odds ratios (ORs) with 95% confidence intervals (CIs) were calculated by utilizing random-effects models. The Newcastle-Ottawa Scale (NOS) was used for quality assessment of observational studies, the Cochrane Risk of Bias Tool for Randomized Trials (Rob 2.0) was used for randomized controlled trials, and all the outcomes were assessed by using the Grading of Recommendations, Assessment, Development and Evaluation (GRADE).
    Results: This study included 32 studies comprising 24,724 cancer patients. RA, either alone or in combination with GA, was significantly associated with reduced cancer recurrence compared to GA alone (OR = 0.82; 95% CI = 0.72 to 0.94; p < 0.01). This association remained significant for prostate cancer patients in subgroup analyses (OR = 0.71; 95% CI = 0.51 to 0.98; p = 0.04) and in the context of epidural anesthesia combined with GA. However, there were no significant associations noted for local recurrence or distant metastasis.
    Conclusions: This meta-analysis provides evidence that RA, used alone or adjunctively with GA, is associated with a lower risk of cancer recurrence, particularly in patients with prostate cancer. However, no significant effects were observed on local recurrence or distant metastasis. Further prospective studies should be conducted to clarify this important issue.
    MeSH term(s) Adult ; Male ; Humans ; Prospective Studies ; Anesthesia, Conduction ; Prostatic Neoplasms ; Anesthesia, Epidural ; Anesthesia, General
    Language English
    Publishing date 2024-01-10
    Publishing country England
    Document type Meta-Analysis ; Journal Article
    ZDB-ID 2091252-3
    ISSN 1471-2253 ; 1471-2253
    ISSN (online) 1471-2253
    ISSN 1471-2253
    DOI 10.1186/s12871-023-02400-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Dual-Responsive Nanogels with Cascaded Gentamicin Release and Lysosomal Escape to Combat Intracellular Small Colony Variants for Peritonitis and Sepsis Therapies.

    Xie, Shuang / Li, Yu / Cao, Wenxiong / Peng, Jiawen / Huang, Kun / Meng, Jie / Li, Xiaohong

    Advanced healthcare materials

    2024  , Page(s) e2303671

    Abstract: Intracellular bacteria are the major cause of serious infections including sepsis and peritonitis, but face great challenges in fighting against the stubborn intracellular small colony variants (SCVs). Herein, the authors have developed nanogels (NGs) to ...

    Abstract Intracellular bacteria are the major cause of serious infections including sepsis and peritonitis, but face great challenges in fighting against the stubborn intracellular small colony variants (SCVs). Herein, the authors have developed nanogels (NGs) to destroy both planktonic bacteria and SCVs and eliminate excessive inflammations for peritonitis and sepsis therapies. Free gentamicin (GEN) and hydroxyapatite nanoparticles (NPs) with GEN loading and mannose grafts (mHA
    Language English
    Publishing date 2024-02-28
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2649576-4
    ISSN 2192-2659 ; 2192-2640
    ISSN (online) 2192-2659
    ISSN 2192-2640
    DOI 10.1002/adhm.202303671
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: SC-Track: a robust cell-tracking algorithm for generating accurate single-cell lineages from diverse cell segmentations.

    Li, Chengxin / Xie, Shuang Shuang / Wang, Jiaqi / Sharvia, Septavera / Chan, Kuan Yoow

    Briefings in bioinformatics

    2024  Volume 25, Issue 3

    Abstract: Computational analysis of fluorescent timelapse microscopy images at the single-cell level is a powerful approach to study cellular changes that dictate important cell fate decisions. Core to this approach is the need to generate reliable cell ... ...

    Abstract Computational analysis of fluorescent timelapse microscopy images at the single-cell level is a powerful approach to study cellular changes that dictate important cell fate decisions. Core to this approach is the need to generate reliable cell segmentations and classifications necessary for accurate quantitative analysis. Deep learning-based convolutional neural networks (CNNs) have emerged as a promising solution to these challenges. However, current CNNs are prone to produce noisy cell segmentations and classifications, which is a significant barrier to constructing accurate single-cell lineages. To address this, we developed a novel algorithm called Single Cell Track (SC-Track), which employs a hierarchical probabilistic cache cascade model based on biological observations of cell division and movement dynamics. Our results show that SC-Track performs better than a panel of publicly available cell trackers on a diverse set of cell segmentation types. This cell-tracking performance was achieved without any parameter adjustments, making SC-Track an excellent generalized algorithm that can maintain robust cell-tracking performance in varying cell segmentation qualities, cell morphological appearances and imaging conditions. Furthermore, SC-Track is equipped with a cell class correction function to improve the accuracy of cell classifications in multiclass cell segmentation time series. These features together make SC-Track a robust cell-tracking algorithm that works well with noisy cell instance segmentation and classification predictions from CNNs to generate accurate single-cell lineages and classifications.
    MeSH term(s) Cell Tracking/methods ; Algorithms ; Single-Cell Analysis/methods ; Cell Lineage ; Humans ; Image Processing, Computer-Assisted/methods ; Neural Networks, Computer ; Deep Learning ; Microscopy, Fluorescence/methods
    Language English
    Publishing date 2024-05-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 2068142-2
    ISSN 1477-4054 ; 1467-5463
    ISSN (online) 1477-4054
    ISSN 1467-5463
    DOI 10.1093/bib/bbae192
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Effects of Aromatherapy on Physical and Mental Health of Cancer Patients Undergoing Radiotherapy and/or Chemotherapy: A Meta-Analysis.

    Xie, Shuang-Ran / Ma, Liang / Xu, Xin-Yu / Zhou, Shu / Xie, Hui-Miao / Xie, Chang-Sheng

    Chinese journal of integrative medicine

    2024  Volume 30, Issue 5, Page(s) 449–457

    Abstract: Backgroup: Currently, aromatherapy is being increasingly utilized in clinical practice, particularly in managing the side effects associated with radiotherapy and chemoradiotherapy. However, it remains to be established whether aromatherapy can ... ...

    Abstract Backgroup: Currently, aromatherapy is being increasingly utilized in clinical practice, particularly in managing the side effects associated with radiotherapy and chemoradiotherapy. However, it remains to be established whether aromatherapy can effectively alleviate these symptoms.
    Objective: To investigate the effects of aromatherapy on the physical and mental health of patients with cancer undergoing radiotherapy and chemotherapy.
    Methods: Seven databases were researched from inception until September 29, 2023, including PubMed, Scopus, and Web of Science, Chinese National Knowledge Infrastructure, Wanfang database, China Biology Medicine disc and VIP Chinese Medical Journal Database. Review Manager version 5.3 was utilized for data analysis. The Cochrane Risk of Bias tool RoB2 was employed to evaluate the quality of the literature included in the study. Evidence quality rating was assessed using the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) approach through the GRADEpro GDT online tool.
    Results: Nineteen studies involving 1,541 patients were included. Aromatherapy can alleviate nausea [relative risk (RR)=0.64, 95% confidence interval (CI): 0.53 to 0.78, P<0.05, I
    Conclusions: Aromatherapy is an efficacious, safe and economic adjunctive therapy for cancer patients, which can mend the physical symptoms and mental health of cancer patients. However, more high-quality studies are needed to verify it. (PROSPERO registration No. CRD42023390171).
    MeSH term(s) Humans ; Aromatherapy/methods ; Mental Health ; Neoplasms/complications ; Neoplasms/psychology ; Neoplasms/radiotherapy ; Neoplasms/therapy ; Quality of Life ; Radiotherapy/adverse effects
    Language English
    Publishing date 2024-03-15
    Publishing country China
    Document type Journal Article ; Meta-Analysis ; Systematic Review
    ZDB-ID 2171254-2
    ISSN 1993-0402 ; 1672-0415
    ISSN (online) 1993-0402
    ISSN 1672-0415
    DOI 10.1007/s11655-024-3659-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Etomidate affects spatial learning and memory and neuronal apoptosis of rats via MAPK/ERK pathway.

    Xie, Shuang / Li, Xuanfa / Xie, Hong

    American journal of translational research

    2022  Volume 14, Issue 8, Page(s) 5823–5832

    Abstract: Objective: To observe the effect of etomidate on spatial learning and memory and neuronal apoptosis in rats of different ages.: Methods: The rats of different ages were divided into 3 age groups: juvenile (21-day old), adult (~3-months old) and ... ...

    Abstract Objective: To observe the effect of etomidate on spatial learning and memory and neuronal apoptosis in rats of different ages.
    Methods: The rats of different ages were divided into 3 age groups: juvenile (21-day old), adult (~3-months old) and elderly (~19-months old). Then, rats with similar age within a group were randomly divided into three subgroups, with 10 rats in each group. The experimental subgroups were intraperitoneally injected with etomidate (emulsion formulated,
    Results: Etomidate significantly extended the escape latency and reduced the platform crossings and the swimming time at original platform of juvenile rats, indicating that the spatial learning and memory function of juvenile rats can be affected by etomidate. However, etomidate had no effect on spatial learning and memory in adult and elderly rats. There were no obvious abnormalities in number of neurons and morphology of vertebral cells in the hippocampus of all experimental subgroups when compared with those of corresponding blank control subgroups. There was no statistically significant difference in apoptosis index of the hippocampal tissue between the experimental subgroups and corresponding blank control subgroups (
    Conclusion: Etomidate may have certain effects on spatial learning and memory in juvenile rats but not in adult and elderly rats. Etomidate affects neither the number of neurons in the CA1 area of the hippocampus nor the morphology of vertebral cells and did not cause the apoptosis of nerve cells. The mechanism of etomidate influence on the spatial learning and memory function of young rats may connect with the inhibition of MAPK/ERK pathway.
    Language English
    Publishing date 2022-08-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2471058-1
    ISSN 1943-8141
    ISSN 1943-8141
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: An updated view on the centrosome as a cell cycle regulator.

    Lin, Muyang / Xie, Shuang Shuang / Chan, Kuan Yoow

    Cell division

    2022  Volume 17, Issue 1, Page(s) 1

    Abstract: The centrosome is a multifunctional organelle that is known primarily for its microtubule organising function. Centrosomal defects caused by changes in centrosomal structure or number have been associated with human diseases ranging from congenital ... ...

    Abstract The centrosome is a multifunctional organelle that is known primarily for its microtubule organising function. Centrosomal defects caused by changes in centrosomal structure or number have been associated with human diseases ranging from congenital defects to cancer. We are only beginning to appreciate how the non-microtubule organising roles of the centrosome are related to these clinical conditions. In this review, we will discuss the historical evidence that led to the proposal that the centrosome participates in cell cycle regulation. We then summarize the body of work that describes the involvement of the mammalian centrosome in triggering cell cycle progression and checkpoint signalling. Then we will highlight work from the fission yeast model organism, revealing the molecular details that explain how the spindle pole body (SPB, the yeast functional equivalent of the centrosome), participates in these cell cycle transitions. Importantly, we will discuss some of the emerging questions from recent discoveries related to the role of the centrosome as a cell cycle regulator.
    Language English
    Publishing date 2022-02-14
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2236097-9
    ISSN 1747-1028
    ISSN 1747-1028
    DOI 10.1186/s13008-022-00077-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Pyroelectric Janus nanomotors to promote cell internalization and synergistic tumor therapy

    Meng, Jie / Wei, Kun / Xie, Shuang / Zhang, Zhanlin / Ran, Pan / Zhang, Peng / Li, Xiaohong

    Journal of Controlled Release. 2023 May, v. 357 p.342-355

    2023  

    Abstract: The tumor diffusion and cell internalization are the major obstacles to improving delivery efficacy of therapeutic agents. External electric fields have shown strong effect on the cell membrane polarization and fluidity, but usually need complicated ... ...

    Abstract The tumor diffusion and cell internalization are the major obstacles to improving delivery efficacy of therapeutic agents. External electric fields have shown strong effect on the cell membrane polarization and fluidity, but usually need complicated power management circuits. Herein, in situ generation of microelectric field on nanoparticles (NPs) is proposed to overcome these delivery barriers. Janus tBT@PDA-CPT NPs were developed through partially coating of polydopamine (PDA) caps on pyroelectric tetragonal BaTiO₃ (tBT) NPs and then camptothecin (CPT) conjugation via disulfide linkages. For comparison, cBT@PDA-CPT NPs were prepared from non-pyroelectric cubic BaTiO₃ (cBT) as control. Near-infrared (NIR) illumination on PDA caps of the Janus NPs produces asymmetric thermophoretic force to drive NP motion for tumor accumulation, deep tissue penetration and effective cell interaction. Photothermally created temperature variations on tBT NPs build pyroelectric potentials to selectively change the membrane potential of tumor cells other than normal cells and exhibit a dominated role in enhancing tumor cell internalization and cytotoxicity. The combination index analysis confirms the synergistic effect of pyroelectric dynamic therapy (PEDT), chemotherapy and photothermal therapy (PTT), leading to full inhibition of tumor growth and noticeable extension of animal survival at significant lower CPT doses. The mild PTT/PEDT, the reduced CPT dose and the selective toxicity to tumor cells have achieved favorable treatment safety after tBT@PDA-CPT/NIR treatment. Therefore, in response to the differences in membrane potentials and glutathione levels between tumor and normal cells, we have demonstrated a concise design to achieve thermophoresis-driven motion, pyroelectric potential-enhanced cell internalization and PTT/PEDT/chemotherapy-synergized antitumor treatment.
    Keywords animals ; cell membranes ; cytotoxicity ; disulfides ; drug therapy ; glutathione ; lighting ; membrane potential ; nanoparticles ; neoplasm cells ; neoplasms ; photothermotherapy ; synergism ; temperature ; Pyroelectric field ; Cell membrane potential ; Thermophoresis-driven motion ; Pyroelectric dynamic therapy ; Synergistic effect
    Language English
    Dates of publication 2023-05
    Size p. 342-355.
    Publishing place Elsevier B.V.
    Document type Article ; Online
    ZDB-ID 632533-6
    ISSN 1873-4995 ; 0168-3659
    ISSN (online) 1873-4995
    ISSN 0168-3659
    DOI 10.1016/j.jconrel.2023.04.007
    Database NAL-Catalogue (AGRICOLA)

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  9. Article ; Online: Compensation for Process and Temperature Dependency in a CMOS Image Sensor.

    Xie, Shuang / Theuwissen, Albert

    Sensors (Basel, Switzerland)

    2019  Volume 19, Issue 4

    Abstract: This paper analyzes and compensates for process and temperature dependency among a (Complementary Metal Oxide Semiconductor) CMOS image sensor (CIS) array. Both the analysis and compensation are supported with experimental results on the CIS's dark ... ...

    Abstract This paper analyzes and compensates for process and temperature dependency among a (Complementary Metal Oxide Semiconductor) CMOS image sensor (CIS) array. Both the analysis and compensation are supported with experimental results on the CIS's dark current, dark signal non-uniformity (DSNU), and conversion gain (CG). To model and to compensate for process variations, process sensors based on pixel source follower (SF)'s transconductance
    Language English
    Publishing date 2019-02-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2052857-7
    ISSN 1424-8220 ; 1424-8220
    ISSN (online) 1424-8220
    ISSN 1424-8220
    DOI 10.3390/s19040870
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Dumbbell Dual-Hairpin Triggered DNA Nanonet Assembly for Cascade-Amplified Sensing of Exosomal MicroRNA.

    Li, Yongxing / Tang, Xiaoqi / Deng, Ruijia / Feng, Liu / Xie, Shuang / Chen, Ming / Zheng, Ji / Chang, Kai

    ACS omega

    2024  Volume 9, Issue 17, Page(s) 19723–19731

    Abstract: Exosomal microRNAs (miRNAs) are valuable biomarkers closely associated with cancer progression. Therefore, sensitive and specific exosomal miRNA biosensing has been employed for cancer diagnosis, prognosis, and prediction. In this study, a miRNA-based ... ...

    Abstract Exosomal microRNAs (miRNAs) are valuable biomarkers closely associated with cancer progression. Therefore, sensitive and specific exosomal miRNA biosensing has been employed for cancer diagnosis, prognosis, and prediction. In this study, a miRNA-based DNA nanonet assembly strategy is proposed, enabling the biosensing of exosomal miRNAs through dumbbell dual-hairpin under isothermal enzyme-free conditions. This strategy dexterously designs a specific dumbbell dual-hairpin that can selectively recognize exosomal miRNA, inducing conformational changes to cascade-generated X-shaped DNA structures, facilitating the extension of the X-shaped DNA in three-dimensional space, ultimately forming a DNA nanonet assembly. On the basis of the target miRNA, our design enriches the fluorescence signal through the cascade assembly of DNA nanonet and realizes the secondary signal amplification. Using exosomal miR-141 as the target, the resultant fluorescence sensing demonstrates an impressive detection limit of 57.6 pM and could identify miRNA sequences with single-base variants with high specificity. Through the analysis of plasma and urine samples, this method effectively distinguishes between benign prostatic hyperplasia, prostate cancer, and metastatic prostate cancer. Serving as a novel noninvasive and accurate screening and diagnostic tool for prostate cancer, this dumbbell dual-hairpin triggered DNA nanonet assembly strategy is promising for clinical applications.
    Language English
    Publishing date 2024-04-22
    Publishing country United States
    Document type Journal Article
    ISSN 2470-1343
    ISSN (online) 2470-1343
    DOI 10.1021/acsomega.4c02652
    Database MEDical Literature Analysis and Retrieval System OnLINE

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