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Article: Building the toolbox for in vivo glia-to-neuron reprogramming.

Xie, Ye / Chen, Bo

2023 Volume 19, Issue 6, Page(s) 1171–1172

Language	English
Publishing date	2023-09-22
Publishing country	India
Document type	Journal Article
ZDB-ID	2388460-5
ISSN	1876-7958 ; 1673-5374
ISSN (online)	1876-7958
ISSN	1673-5374
DOI	10.4103/1673-5374.385869
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Critical Examination of Müller Glia-Derived

Xie, Ye / Chen, Bo

Frontiers in cell and developmental biology

2022 Volume 10, Page(s) 830382

Abstract: Müller glia (MG) are a potential source of stem cells in the mammalian retina that could replenish lost retinal neurons for vision restoration. Unlike their counterpart in zebrafish, mammalian MG are quiescent and they do not spontaneously generate new ... ...

Abstract	Müller glia (MG) are a potential source of stem cells in the mammalian retina that could replenish lost retinal neurons for vision restoration. Unlike their counterpart in zebrafish, mammalian MG are quiescent and they do not spontaneously generate new retinal neurons. In recent years, extensive research efforts have been made to unlock the regenerative capabilities of Müller glia (MG) for
Language	English
Publishing date	2022-03-31
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2737824-X
ISSN	2296-634X
ISSN	2296-634X
DOI	10.3389/fcell.2022.830382
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Article ; Online: The Dual-Mode Transition of Myofibroblasts Derived from Hepatic Stellate Cells in Liver Fibrosis.

Yan, Mengchao / Xie, Ye / Yao, Jia / Li, Xun

International journal of molecular sciences

2023 Volume 24, Issue 20

Abstract: Hepatic stellate cells (HSCs) are the key promoters of liver fibrosis. In response to liver-fibrosis-inducing factors, HSCs express alpha smooth muscle actin (α-SMA) and obtain myofibroblast phenotype. Collagen secretion and high expression of α-SMA with ...

Abstract	Hepatic stellate cells (HSCs) are the key promoters of liver fibrosis. In response to liver-fibrosis-inducing factors, HSCs express alpha smooth muscle actin (α-SMA) and obtain myofibroblast phenotype. Collagen secretion and high expression of α-SMA with related high cell tension and migration limitation are the main characteristics of myofibroblasts. How these two characteristics define the role of myofibroblasts in the initiation and progression of liver fibrosis is worth exploring. From this perspective, we explored the correlation between α-SMA expression and collagen secretion in myofibroblasts and the characteristics of collagen deposition in liver fibrosis. Based on a reasonable hypothesis and experimental verification, we believe that the myofibroblast with the α-SMA
MeSH term(s)	Humans ; Myofibroblasts/metabolism ; Hepatic Stellate Cells/metabolism ; Liver Cirrhosis/metabolism ; Collagen/metabolism ; Transforming Growth Factor beta/metabolism ; Actins/metabolism ; Transforming Growth Factor beta1/metabolism ; Liver/metabolism
Chemical Substances	Collagen (9007-34-5) ; Transforming Growth Factor beta ; Actins ; Transforming Growth Factor beta1
Language	English
Publishing date	2023-10-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms242015460
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Article ; Online: Oxygen glucose deprivation-pretreated astrocyte-derived exosomes attenuates intracerebral hemorrhage (ICH)-induced BBB disruption through miR-27a-3p /ARHGAP25/Wnt/β-catenin axis.

Hou, Ying / Xie, Ye / Liu, Xiaoxuan / Chen, Yushan / Zhou, Fangfang / Yang, Binbin

Fluids and barriers of the CNS

2024 Volume 21, Issue 1, Page(s) 8

Abstract: Background: Blood brain barrier (BBB) breakdown is one of the key mechanisms of secondary brain injury following intracerebral hemorrhage (ICH). Astrocytes interact with endothelial and regulate BBB integrity via paracrine signaling factors. More and ... ...

Abstract	Background: Blood brain barrier (BBB) breakdown is one of the key mechanisms of secondary brain injury following intracerebral hemorrhage (ICH). Astrocytes interact with endothelial and regulate BBB integrity via paracrine signaling factors. More and more studies reveal astrocyte-derived extracellular vesicles (ADEVs) as an important way of intercellular communication. However, the role of ADEV in BBB integrity after ICH remains unclear. Methods: ADEVs were obtained from astrocytes with or without oxygen and glucose deprivation (OGD) pre-stimulation and the role of ADEVs in ICH was investigated using ICH mice model and ICH cell model. The potential regulatory effect of ADEVs on endothelial barrier integrity was identified by TEER, western blot and immunofluorescence in vitro. In vivo, functional evaluation, Evans-blue leakage and tight junction proteins (TJPs) expression were analyzed. MiRNA sequencing revealed that microRNA-27a-3p (miR-27a-3p) was differentially expressed miRNA in the EVs from OGD-pretreated astrocytes compared with normal control. The regulatory mechanism of miR-27a-3p was assessed using Luciferase assay, RT-PCR, western blot and immunofluorescence. Results: OGD-activated astrocytes reduced hemin-induced endothelial hyper-permeability through secreting EVs. OGD-activated ADEVs alleviated BBB dysfunction after ICH in vivo and in vitro. MicroRNA microarray analysis indicated that miR-27a-3p is a major component that was highly expressed miRNA in OGD pretreated-ADEVs. OGD-ADEVs mitigated BBB injury through transferring miR-27a-3p into bEnd.3 cells and regulating ARHGAP25/Wnt/β-catenin pathway. Conclusion: Taken together, these findings firstly revealed that miR-27a-3p, as one of the main components of OGD-pretreated ADEVs, attenuated BBB destruction and improved neurological deficits following ICH by regulating endothelial ARHGAP25/Wnt/β-catenin axis. OGD-ADEVs might be a novel strategy for the treatment of ICH. this study implicates that EVs from OGD pre-stimulated astrocytes.
MeSH term(s)	Animals ; Mice ; Blood-Brain Barrier/metabolism ; Astrocytes/metabolism ; beta Catenin/metabolism ; Endothelial Cells/metabolism ; Exosomes/metabolism ; Oxygen/metabolism ; Glucose ; MicroRNAs/genetics ; MicroRNAs/metabolism ; MicroRNAs/pharmacology ; Cerebral Hemorrhage/metabolism
Chemical Substances	MIRN27 microRNA, rat ; beta Catenin ; Oxygen (S88TT14065) ; Glucose (IY9XDZ35W2) ; MicroRNAs
Language	English
Publishing date	2024-01-19
Publishing country	England
Document type	Journal Article
ZDB-ID	2595406-4
ISSN	2045-8118 ; 2045-8118
ISSN (online)	2045-8118
ISSN	2045-8118
DOI	10.1186/s12987-024-00510-2
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Article ; Online: Bioreactor-based stem cell therapy for liver fibrosis.

Yan, Mengchao / Yao, Jia / Xie, Ye / Jiang, Pan / Yan, Jun / Li, Xun

Biofabrication

2024 Volume 16, Issue 2

Abstract: Stem cell therapy, achieved using mesenchymal stem cells (MSCs), has been highlighted for the treatment of liver fibrosis. Infusion into the circulatory system is a traditional application of MSCs; however, this approach is limited by phenotypic drift, ... ...

Abstract	Stem cell therapy, achieved using mesenchymal stem cells (MSCs), has been highlighted for the treatment of liver fibrosis. Infusion into the circulatory system is a traditional application of MSCs; however, this approach is limited by phenotypic drift, stem cell senescence, and vascular embolism. Maintaining the therapeutic phenotype of MSCs while avoiding adverse infusion-related reactions is the key to developing next-generation stem cell therapy technologies. Here, we propose a bioreactor-based MSCs therapy to avoid cell infusion. In this scheme, 5% liver fibrosis serum was used to induce the therapeutic phenotype of MSCs, and a fluid bioreactor carrying a co-culture system of hepatocytes and MSCs was constructed to produce the therapeutic medium. In a rat model of liver fibrosis, the therapeutic medium derived from the bioreactor significantly alleviated liver fibrosis. Therapeutic mechanisms include immune regulation, inhibition of hepatic stellate cell activation, establishment of hepatocyte homeostasis, and recovery of liver stem cell subsets. Overall, the bioreactor-based stem cell therapy (scheme) described here represents a promising new strategy for the treatment of liver fibrosis and will be beneficial for the development of 'cell-free' stem cell therapy.
MeSH term(s)	Rats ; Animals ; Liver ; Mesenchymal Stem Cell Transplantation ; Liver Cirrhosis/therapy ; Liver Cirrhosis/pathology ; Hepatocytes ; Mesenchymal Stem Cells ; Fibrosis
Language	English
Publishing date	2024-03-14
Publishing country	England
Document type	Journal Article
ZDB-ID	2500944-8
ISSN	1758-5090 ; 1758-5082
ISSN (online)	1758-5090
ISSN	1758-5082
DOI	10.1088/1758-5090/ad304d
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Article ; Online: Critical examination of Ptbp1-mediated glia-to-neuron conversion in the mouse retina.

Xie, Ye / Zhou, Jing / Chen, Bo

Cell reports

2022 Volume 39, Issue 11, Page(s) 110960

Abstract: Reprogramming glial cells to convert them into neurons represents a potential therapeutic strategy that could repair damaged neural circuits and restore function. Recent studies show that downregulation of the RNA-binding protein PTBP1 leads to one-step ... ...

Abstract	Reprogramming glial cells to convert them into neurons represents a potential therapeutic strategy that could repair damaged neural circuits and restore function. Recent studies show that downregulation of the RNA-binding protein PTBP1 leads to one-step conversion of Müller glia (MG) into retinal ganglion cells (RGCs) with a high efficiency. However, the original study did not perform fate-mapping experiments to confirm MG-to-RGC conversion after Ptbp1 downregulation. To address the fundamental question of whether Ptbp1 downregulation can convert MG into RGCs in the mouse retina, we perform fate-mapping experiments to lineage trace MG independent of the adeno-associated virus (AAV)-mediated labeling system. Here, we report that Ptbp1 downregulation by CRISPR-CasRx or small hairpin RNA is insufficient to convert MG to RGCs. The original conclusion of MG-to-RGC conversion is due to leaky labeling of endogenous RGCs. Our results emphasize the importance of using stringent fate mapping to determine glia-to-neuron conversion in cell reprogramming research.
MeSH term(s)	Animals ; Cellular Reprogramming/genetics ; Dependovirus ; Heterogeneous-Nuclear Ribonucleoproteins/metabolism ; Mice ; Neuroglia/metabolism ; Polypyrimidine Tract-Binding Protein/genetics ; Polypyrimidine Tract-Binding Protein/metabolism ; RNA-Binding Proteins/metabolism ; Retina ; Retinal Ganglion Cells/metabolism
Chemical Substances	Heterogeneous-Nuclear Ribonucleoproteins ; Ptbp1 protein, mouse ; RNA-Binding Proteins ; Polypyrimidine Tract-Binding Protein (139076-35-0)
Language	English
Publishing date	2022-06-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	2649101-1
ISSN	2211-1247 ; 2211-1247
ISSN (online)	2211-1247
ISSN	2211-1247
DOI	10.1016/j.celrep.2022.110960
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Article ; Online: Primary cemented hemiarthroplasty for unstable intertrochanteric fractures in elderly severe osteoporotic patients.

Xie, Ye / Zhou, Haibin

Injury

2020 Volume 51, Issue 3, Page(s) 670–673

Abstract: Objective: To evaluate the outcome of unstable intertrochanteric fractures with primary cemented hemiarthroplasty (HA) in elderly severe osteoporotic patients.: Material and methods: In January 2009 to July 2017, 277 patients were selected according ... ...

Abstract	Objective: To evaluate the outcome of unstable intertrochanteric fractures with primary cemented hemiarthroplasty (HA) in elderly severe osteoporotic patients. Material and methods: In January 2009 to July 2017, 277 patients were selected according to inclusion criteria and results were analyzed prospectively. According to AO/OTA Classification, only type 31-A2.2(148 cases) and 31-A2.3(129cases) were included. 53 cases were males and 224 cases were females. The mean age was 75.4 years. The mean follow-up period was 50 months. The Harris Hip Score (HHS) and perioperative index (including the time duration of the surgery, intraoperative blood loss, postoperatively weight bearing time, implant complications) were analyzed clinically. Results: Follow-up evaluations were performed at 6 weeks, 3, 6, 9 and 12 months, and every year thereafter (mean 50 months). The average duration of surgery was 43.2 min (range 21-65 min), with a mean blood loss of 225.8 ml (range 70-425 ml). All patients were out of the bed and mobile with help of walker with average of 3.3 days (range 1-5 days). The average duration of hospital stay was 12.3 days. Average HHS improved from 83.7 (range 63-90) at 6 months follow up to 90.3 (range 74-92) at final follow up. Conclusion: For the severe osteoporotic elderly with unstable fractures, bipolar hemiarthroplasty is an effective method to treat the unstable intertrochanteric fractures in the severe osteoporotic elderly. It can decrease complications, reduce mortality, improve the patient's life quality, and reduce family burden. In Summation, when deciding on treatment methods for intertrochanteric fractures several factors must be considered, which are (included but not limited to) the type of fracture, the patient's age, health status, severity of osteoporosis. As long as there was no absolute contraindication for surgery, it is appropriate to have surgery and to restore function as early as possible.
MeSH term(s)	Aged ; Aged, 80 and over ; Arthroplasty, Replacement, Hip/methods ; China ; Female ; Hemiarthroplasty/methods ; Hip Fractures/surgery ; Hip Prosthesis ; Humans ; Length of Stay ; Male ; Middle Aged ; Operative Time ; Osteoporotic Fractures/surgery ; Prospective Studies ; Range of Motion, Articular ; Recovery of Function ; Treatment Outcome
Language	English
Publishing date	2020-01-07
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	218778-4
ISSN	1879-0267 ; 0020-1383
ISSN (online)	1879-0267
ISSN	0020-1383
DOI	10.1016/j.injury.2020.01.010
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Article ; Online: Practical application of the patient data-based quality control method: the potassium example.

Zhang, Yan / Wang, Hua-Li / Xie, Ye-Hong / He, Da-Hai / Zhou, Chao-Qiong / Kong, Li-Rui

Biochemia medica

2024 Volume 34, Issue 1, Page(s) 10901

Abstract: Introduction: Internal quality control (IQC) is a core pillar of laboratory quality control strategies. Internal quality control commercial materials lack the same characteristics as patient samples and IQC contributes to the costs of laboratory testing. ...

Abstract	Introduction: Internal quality control (IQC) is a core pillar of laboratory quality control strategies. Internal quality control commercial materials lack the same characteristics as patient samples and IQC contributes to the costs of laboratory testing. Patient data-based quality control (PDB-QC) may be a valuable supplement to IQC; the smaller the biological variation, the stronger the ability to detect errors. Using the potassium concentration in serum as an example study compared error detection effectiveness between PDB-QC and IQC. Materials and methods: Serum potassium concentrations were measured by using an indirect ion-selective electrode method. For the training database, 23,772 patient-generated data and 366 IQC data from April 2022 to September 2022 were used; 15,351 patient-generated data and 246 IQC data from October 2022 to January 2023 were used as the testing database. For both PDB-QC and IQC, average values and standard deviations were calculated, and z-score charts were plotted for comparison purposes. Results: Five systematic and three random errors were detected using IQC. Nine systematic errors but no random errors were detected in PDB-QC. The PDB-QC showed systematic error warnings earlier than the IQC. Conclusions: The daily average value of patient-generated data was superior to IQC in terms of the efficiency and timeliness of detecting systematic errors but inferior to IQC in detecting random errors.
MeSH term(s)	Humans ; Quality Control ; Laboratories
Language	English
Publishing date	2024-02-01
Publishing country	Croatia
Document type	Journal Article
ZDB-ID	1208725-7
ISSN	1846-7482 ; 1330-0962
ISSN (online)	1846-7482
ISSN	1330-0962
DOI	10.11613/BM.2024.010901
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Article ; Online: Stem cells for treatment of liver fibrosis/cirrhosis: clinical progress and therapeutic potential.

Liu, Pinyan / Mao, Yongcui / Xie, Ye / Wei, Jiayun / Yao, Jia

Stem cell research & therapy

2022 Volume 13, Issue 1, Page(s) 356

Abstract: Cost-effective treatment strategies for liver fibrosis or cirrhosis are limited. Many clinical trials of stem cells for liver disease shown that stem cells might be a potential therapeutic approach. This review will summarize the published clinical ... ...

Abstract	Cost-effective treatment strategies for liver fibrosis or cirrhosis are limited. Many clinical trials of stem cells for liver disease shown that stem cells might be a potential therapeutic approach. This review will summarize the published clinical trials of stem cells for the treatment of liver fibrosis/cirrhosis and provide the latest overview of various cell sources, cell doses, and delivery methods. We also describe the limitations and strengths of various stem cells in clinical applications. Furthermore, to clarify how stem cells play a therapeutic role in liver fibrosis, we discuss the molecular mechanisms of stem cells for treatment of liver fibrosis, including liver regeneration, immunoregulation, resistance to injury, myofibroblast repression, and extracellular matrix degradation. We provide a perspective for the prospects of future clinical implementation of stem cells.
MeSH term(s)	Fibrosis ; Humans ; Liver/metabolism ; Liver Cirrhosis/pathology ; Liver Regeneration/physiology ; Mesenchymal Stem Cell Transplantation/methods
Language	English
Publishing date	2022-07-26
Publishing country	England
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2548671-8
ISSN	1757-6512 ; 1757-6512
ISSN (online)	1757-6512
ISSN	1757-6512
DOI	10.1186/s13287-022-03041-5
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Article ; Online: Deep learning in CT image segmentation of cervical cancer: a systematic review and meta-analysis.

Yang, Chongze / Qin, Lan-Hui / Xie, Yu-En / Liao, Jin-Yuan

Radiation oncology (London, England)

2022 Volume 17, Issue 1, Page(s) 175

Abstract: Background: This paper attempts to conduct a systematic review and meta-analysis of deep learning (DLs) models for cervical cancer CT image segmentation.: Methods: Relevant studies were systematically searched in PubMed, Embase, The Cochrane Library, ...

Abstract	Background: This paper attempts to conduct a systematic review and meta-analysis of deep learning (DLs) models for cervical cancer CT image segmentation. Methods: Relevant studies were systematically searched in PubMed, Embase, The Cochrane Library, and Web of science. The literature on DLs for cervical cancer CT image segmentation were included, a meta-analysis was performed on the dice similarity coefficient (DSC) of the segmentation results of the included DLs models. We also did subgroup analyses according to the size of the sample, type of segmentation (i.e., two dimensions and three dimensions), and three organs at risk (i.e., bladder, rectum, and femur). This study was registered in PROSPERO prior to initiation (CRD42022307071). Results: A total of 1893 articles were retrieved and 14 articles were included in the meta-analysis. The pooled effect of DSC score of clinical target volume (CTV), bladder, rectum, femoral head were 0.86(95%CI 0.84 to 0.87), 0.91(95%CI 0.89 to 0.93), 0.83(95%CI 0.79 to 0.88), and 0.92(95%CI 0.91to 0.94), respectively. For the performance of segmented CTV by two dimensions (2D) and three dimensions (3D) model, the DSC score value for 2D model was 0.87 (95%CI 0.85 to 0.90), while the DSC score for 3D model was 0.85 (95%CI 0.82 to 0.87). As for the effect of the capacity of sample on segmentation performance, no matter whether the sample size is divided into two groups: greater than 100 and less than 100, or greater than 150 and less than 150, the results show no difference (P > 0.05). Four papers reported the time for segmentation from 15 s to 2 min. Conclusion: DLs have good accuracy in automatic segmentation of CT images of cervical cancer with a less time consuming and have good prospects for future radiotherapy applications, but still need public high-quality databases and large-scale research verification.
MeSH term(s)	Female ; Humans ; Deep Learning ; Image Processing, Computer-Assisted/methods ; Uterine Cervical Neoplasms/diagnostic imaging ; Uterine Cervical Neoplasms/radiotherapy ; Tomography, X-Ray Computed/methods ; Pelvis
Language	English
Publishing date	2022-11-07
Publishing country	England
Document type	Meta-Analysis ; Systematic Review ; Journal Article
ZDB-ID	2224965-5
ISSN	1748-717X ; 1748-717X
ISSN (online)	1748-717X
ISSN	1748-717X
DOI	10.1186/s13014-022-02148-6
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