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Article ; Online: Long-term intensive management reduced the soil quality of a Carya dabieshanensis forest

Cheng Huang / Songling Fu / Xiaomin Ma / Xiaoxiang Ma / Xiaoliang Ren / Xinxin Tian / Yinhao Tong / Feiyang Yuan / Hua Liu

Scientific Reports, Vol 13, Iss 1, Pp 1-

2023 Volume 12

Abstract: Abstract The evaluation of soil quality can provide new insights into the sustainable management of forests. This study investigated the effects of three types of forest management intensities (non-management (CK), extensive management (EM), and ... ...

Abstract	Abstract The evaluation of soil quality can provide new insights into the sustainable management of forests. This study investigated the effects of three types of forest management intensities (non-management (CK), extensive management (EM), and intensive management (IM)), and five management durations (0, 3, 8, 15, and 20 years) on the soil quality of a Carya dabieshanensis forest. Further, minimum data sets (MDS) and optimized minimum data sets (OMDS) were established to evaluate the soil quality index (SQI). A total of 20 soil indicators representing its physical, chemical, and biological properties were measured for the 0–30 cm layer. Using one-way ANOVA and principal component analysis (PCA), the total data set (TDS), the minimum data set (MDS), and optimized minimum data set (OMDS) were established. The MDS and OMDS contained three (alkali hydrolyzed nitrogen (AN), soil microbial biomass nitrogen (SMBN), and pH) and four (total phosphorus (TP), soil organic carbon (SOC), AN, and bulk density (BD)) soil indicators, respectively. The SQI derived from the OMDS and TDS exhibited a stronger correlation (r = 0.94, p < 0.01), which was suitable for evaluating the soil quality of the C. dabieshanensis forest. The evaluation results revealed that the soil quality was highest during the early stage of intensive management (IM-3), and the SQI of each soil layer was 0.81 ± 0.13, 0.47 ± 0.11, and 0.38 ± 0.07, respectively. With extended management times, the degree of soil acidification increased, and the nutrient content decreased. Compared with the untreated forest land the soil pH, SOC, and TP decreased by 2.64–6.24%, 29.43–33.04%, and 43.63–47.27%, respectively, following 20 years of management, while the SQI of each soil layer decreased to 0.35 ± 0.09, 0.16 ± 0.02 and 0.12 ± 0.06, respectively. In contrast to extensive management, the soil quality deteriorated more rapidly under longer management and intensive supervision. The OMDS established in this study provides a reference for the assessment of soil ...
Keywords	Medicine ; R ; Science ; Q
Subject code	630 ; 550
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Stability Analysis for Viral Infection Model with Multitarget Cells, Beddington-DeAngelis Functional Response, and Humoral Immunity

Xinxin Tian / Jinliang Wang

Discrete Dynamics in Nature and Society, Vol

2015 Volume 2015

Abstract: We formulate a (2n+2)-dimensional viral infection model with humoral immunity, n classes of uninfected target cells and n classes of infected cells. The incidence rate of infection is given by nonlinear incidence rate, Beddington-DeAngelis functional ... ...

Abstract	We formulate a (2n+2)-dimensional viral infection model with humoral immunity, n classes of uninfected target cells and n classes of infected cells. The incidence rate of infection is given by nonlinear incidence rate, Beddington-DeAngelis functional response. The model admits discrete time delays describing the time needed for infection of uninfected target cells and virus replication. By constructing suitable Lyapunov functionals, we establish that the global dynamics are determined by two sharp threshold parameters: R0 and R1. Namely, a typical two-threshold scenario is shown. If R0≤1, the infection-free equilibrium P0 is globally asymptotically stable, and the viruses are cleared. If R1≤1<R0, the immune-free equilibrium P1 is globally asymptotically stable, and the infection becomes chronic but with no persistent antibody immune response. If R1>1, the endemic equilibrium P2 is globally asymptotically stable, and the infection is chronic with persistent antibody immune response.
Keywords	Mathematics ; QA1-939 ; Social sciences (General) ; H1-99
Subject code	570
Language	English
Publishing date	2015-01-01T00:00:00Z
Publisher	Hindawi Publishing Corporation
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Stability Analysis for Viral Infection Model with Multitarget Cells, Beddington-DeAngelis Functional Response, and Humoral Immunity

Xinxin Tian / Jinliang Wang

Discrete Dynamics in Nature and Society, Vol

2014 Volume 2014

Keywords	Social sciences (General) ; H1-99 ; Social Sciences ; H ; Mathematics ; QA1-939 ; Science ; Q
Language	English
Publishing date	2014-01-01T00:00:00Z
Publisher	Hindawi Publishing Corporation
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Global stability of a delay differential equation of hepatitis B virus infection with immune response

Jinliang Wang / Xinxin Tian

Electronic Journal of Differential Equations, Vol 2013, Iss 94,, Pp 1-

2013 Volume 11

Abstract: The global stability for a delayed HBV infection model with CTL immune response is investigated. We show that the global dynamics is determined by two sharp thresholds, basic reproduction number $Re_0$ and CTL immune-response reproduction number $Re_1$. ... ...

Abstract	The global stability for a delayed HBV infection model with CTL immune response is investigated. We show that the global dynamics is determined by two sharp thresholds, basic reproduction number $Re_0$ and CTL immune-response reproduction number $Re_1$. When $Re_0 leq 1$, the infection-free equilibrium is globally asymptotically stable, which means that the viruses are cleared and immune is not active; when $Re_1 leq 1 < Re_0$, the CTL-inactivated infection equilibrium exists and is globally asymptotically stable, which means that CTLs immune response would not be activated and viral infection becomes chronic; and when $Re_1 > 1$, the CTL-activated infection equilibrium exists and is globally asymptotically stable, in this case the infection causes a persistent CTLs immune response. Our model is formulated by incorporating a Cytotoxic T lymphocytes (CTLs) immune response to recent work [Gourley, Kuang, Nagy, J. Bio. Dyn., 2(2008), 140-153] to model the role in antiviral by attacking virus infected cells. Our analysis provides a quantitative understandings of HBV replication dynamics in vivo and has implications for the optimal timing of drug treatment and immunotherapy in chronic HBV infection.
Keywords	HBV infection model ; delay ; CTLs ; global stability ; Mathematics ; QA1-939 ; Science ; Q ; DOAJ:Mathematics ; DOAJ:Mathematics and Statistics
Subject code	570
Language	English
Publishing date	2013-04-01T00:00:00Z
Publisher	Texas State University
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Magnetic solid-phase extraction based on g-C₃N₄/Fe₃O₄/MoS₂ as a magnetic adsorbent for HPLC-UV determination of fluoroquinolones in chicken and eggs

Zhao, Bingxin / Hao Wu / Yanli Liu / Xinxin Tian / Yaoxing Huo / Shuping Guan

Analytical methods. 2019 Mar. 14, v. 11, no. 11

2019

Abstract: A ternary nanocomposite, g-C₃N₄/Fe₃O₄/MoS₂, was successfully synthesized via a reliable and effective hydrothermal method and used as an adsorbent in magnetic solid-phase extraction (MSPE) for separation and concentration of trace fluoroquinolones (FQs) ... ...

Abstract	A ternary nanocomposite, g-C₃N₄/Fe₃O₄/MoS₂, was successfully synthesized via a reliable and effective hydrothermal method and used as an adsorbent in magnetic solid-phase extraction (MSPE) for separation and concentration of trace fluoroquinolones (FQs) in chicken and eggs. This extraction technique is combined with high-performance liquid chromatography (HPLC) using an ultraviolet-visible (UV) detector to determine the content. The synthesized adsorbent was characterized using different techniques, including X-ray diffraction spectrometry (XRD), scanning electron microscopy (SEM), vibrating-sample magnetometry (VSM), transmission electron microscopy (TEM) and Brunauer–Emmett–Teller (BET). Various parameters influencing the extraction efficiency were studied and optimized. Under the optimized conditions, this extraction method provided good linearity over the range of 2–300 μg L⁻¹ with the coefficient of determination, r², ranging from 0.9993 to 0.9997; good spiked recoveries (89.3–99.6%) were obtained under the optimum conditions, and the relative standard deviations, which are used to indicate reproducibility, were less than 4.5%. The limits of detection (LOD) were determined to be in the range from 0.5 to 2.0 μg L⁻¹, and the limits of quantification (LOQ) were from 1.7 to 6.7 μg L⁻¹. This study confirmed the potential of using the g-C₃N₄/Fe₃O₄/MoS₂ adsorbent for extraction of FQs in chicken and eggs.
Keywords	X-ray diffraction ; adsorbents ; chickens ; detection limit ; eggs ; fluoroquinolones ; high performance liquid chromatography ; hot water treatment ; magnetism ; nanocomposites ; scanning electron microscopy ; solid phase extraction ; spectroscopy ; standard deviation ; transmission electron microscopy
Language	English
Dates of publication	2019-0314
Size	p. 1491-1499.
Publishing place	The Royal Society of Chemistry
Document type	Article
ZDB-ID	2515210-5
ISSN	1759-9679 ; 1759-9660
ISSN (online)	1759-9679
ISSN	1759-9660
DOI	10.1039/c9ay00208a
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: HPV prevalence and genotype distribution among women in Shandong Province, China

Lingbo Jiang / Xinxin Tian / Dezhi Peng / Liran Zhang / Fengxiang Xie / Chunrui Bi / Rui Wang / Jiajia Wang / Debo Qi

PLoS ONE, Vol 14, Iss 1, p e

Analysis of 94,489 HPV genotyping results from Shandong's largest independent pathology laboratory.

2019 Volume 0210311

Abstract: Background Data regarding human papillomavirus (HPV) prevalence and genotype distribution are limited in Shandong Province, China. Therefore, we investigated the recent HPV prevalence and genotype distribution among females in Shandong and aimed to ... ...

Abstract	Background Data regarding human papillomavirus (HPV) prevalence and genotype distribution are limited in Shandong Province, China. Therefore, we investigated the recent HPV prevalence and genotype distribution among females in Shandong and aimed to provide comprehensive data to guide HPV-based cervical cancer screening and HPV vaccination for this population of Chinese women. Methods HPV testing results of 94,489 females were retrospectively reviewed and extracted from the database of Jinan KingMed Diagnostics, the largest independent pathology laboratory in Shandong Province, China. HPV was detected by a HPV genotyping panel from January 2011 to June 2017. The overall prevalence, age-specific prevalence, and genotype distribution were analyzed. Results A total of 26,839 cases (28.4%) were HPV-positive, with 4.3% positive for low- or undetermined-risk HPV (lr-/urHPV)-only, 18.1% positive for high-risk HPV (hrHPV)-only, and 6.1% positive for mixed lr-/urHPV and hrHPV infections. Single HPV infections accounted for 62.8%, while the rest were multiple HPV infections of two or more genotypes. HPV16 (5.8%), HPV52 (5.1%), HPV58 (3.5%), HPV51 (2.6%), and HPV56 (2.3%) were the five most common hrHPV genotypes; while HPV81 (2.8%), HPV53 (2.8%), and HPV6 (2.3%) were the three most common lr-/urHPV genotypes. HPV18 (1.7%) was only the ninth most common hrHPV genotype. HPV16 but not HPV52 was more common in single infections than in multiple infections. The distribution of both mixed lr-/urHPV and hrHPV as well as overall HPV infections demonstrated a bimodal pattern across age groups, of which the first peak appeared in the younger group and the second peak was found in older women. A similar age-specific distribution was observed in multiple infections of three or more subtypes as well. Moreover, the proportion of mixed lr-/urHPV and hrHPV infection significantly increased, while those of lr-/urHPV-only and hrHPV-only infections declined as the number of co-infections increased during the study period. Conclusion This ...
Keywords	Medicine ; R ; Science ; Q
Subject code	610
Language	English
Publishing date	2019-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Significant difference between sirolimus and paclitaxel nanoparticles in anti-proliferation effect in normoxia and hypoxia

Youlu Chen / Yong Zeng / Xiaowei Zhu / Lifu Miao / Xiaoyu Liang / Jianwei Duan / Huiyang Li / Xinxin Tian / Liyun Pang / Yongxiang Wei / Jing Yang

Bioactive Materials, Vol 6, Iss 3, Pp 880-

The basis of better selection of atherosclerosis treatment

2021 Volume 889

Abstract: Compared with paclitaxel, sirolimus has been more used in the treatment of vascular restenosis gradually as an anti-proliferative drug, but few basic studies have elucidated its mechanism. The anti-proliferative effects of sirolimus or paclitaxel have ... ...

Abstract	Compared with paclitaxel, sirolimus has been more used in the treatment of vascular restenosis gradually as an anti-proliferative drug, but few basic studies have elucidated its mechanism. The anti-proliferative effects of sirolimus or paclitaxel have been demonstrated by numerous studies under normoxia, but few studies have been achieved focusing hypoxia. In this study, porcine carotid artery injury model and classical cobalt chloride hypoxia cell model were established. Sirolimus nanoparticles (SRM-NPs), paclitaxel nanoparticles (PTX-NPs) and blank nanoparticles (Blank-NPs) were prepared respectively. The effect of RPM-NPs on the degree of stenosis, proliferative index and the expression of PCNA after 28 days of porcine carotid artery injury model was evaluated. Compared with saline group and SRM groups, SRM-NPs group suppressed vascular stenosis, proliferative index and the expression of PCNA (P < 0.01 and P < 0.05). Endothelial cell (EC) and smooth muscle cell (SMC) were pre-treated with cobaltous chloride, followed by SRM-NPs, PTX-NPs, Blank-NPs or PBS control treating, the effects on cell proliferation, HIF-1 expression and glycolysis were detected. SRM-NPs could inhibit EC and SMC proliferation under hypoxia, while PTX-NPs couldn't (P < 0.001). Significant differences between sirolimus and paclitaxel NPs in anti-proliferation effect under normoxia and hypoxia may due to the different inhibitory effects on HIF-1α expression and glycolysis. In conclusion, these results suggest that sirolimus can inhibit the proliferation of hypoxic cells more effectively than paclitaxel. These observations may provide a basis for understanding clinical vascular stenosis therapeutic differences between rapamycin and paclitaxel.
Keywords	Atherosclerosis ; Sirolimus ; Paclitaxel ; Hypoxia ; HIF-1α ; Glycolysis ; Materials of engineering and construction. Mechanics of materials ; TA401-492 ; Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2021-03-01T00:00:00Z
Publisher	KeAi Communications Co., Ltd.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Ameliorative effects of nano-elemental selenium against hexavalent chromium-induced apoptosis in broiler liver

Xueting, Liu / Donghai Zhou / Khalid Mehmood / Mujeeb Ur Rehman / Shucheng Huang / Xiaoxing Wu / Xinxin Tian

Environmental science and pollution research international. 2018 June, v. 25, no. 16

2018

Abstract: The current study examined the ameliorative effects of nano-elemental selenium (Nano-Se) against chromium-VI (K2Cr2O7)-induced apoptosis in chickens. The expression of apoptosis-related genes was evaluated by reverse transcription-quantitative polymerase ...

Abstract	The current study examined the ameliorative effects of nano-elemental selenium (Nano-Se) against chromium-VI (K2Cr2O7)-induced apoptosis in chickens. The expression of apoptosis-related genes was evaluated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot. A total of 60, one-day-old broiler chickens allotted to six equal groups, i.e., control group (standard diet), Cr(VI)-exposed group (K2Cr2O7 via drinking water), Nano-Se group (Nano-Se at 0.5 mg/kg via diet), protection group (K2Cr2O7 + Nano-Se), cure group (K2Cr2O7 for initial 2 weeks and then Nano-Se), and prevention group (opposite to the cure group) and were detected by the activities of pro-apoptosis (Bax, Caspase-3) and anti-apoptosis (Bcl-2) genes expression at day 35 of the experiment. Intense apoptosis was observed in liver tissues of chickens exposed to K2Cr2O7. The Nano-Se supplementation caused a significant decrease (P < 0.01) in the mRNA expression levels of Bax and Caspase-3 genes, while significantly elevated (P < 0.05) mRNA expression level of Bcl-2 gene was observed in Nano-Se experimental groups as compare to control and Cr(VI)-exposed group. The results quantified by the RT-qPCR were further confirmed by the western blot analysis. Altogether, these results suggest anti-apoptotic effects of Nano-Se in the chicken liver, which is interesting for further study. The present findings suggested that Nano-Se has protective effects against K2Cr2O7-induced apoptosis in broilers liver and can serve a key role as a protective agent against apoptosis.
Keywords	apoptosis ; broiler chickens ; caspase-3 ; chromium ; diet ; drinking water ; gene expression ; genes ; liver ; messenger RNA ; protective effect ; quantitative polymerase chain reaction ; reverse transcriptase polymerase chain reaction ; selenium ; tissues ; Western blotting
Language	English
Dates of publication	2018-06
Size	p. 15609-15615.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-018-1758-z
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Previous cervical cytology and high-risk human papillomavirus testing in a cohort of patients with invasive cervical carcinoma in Shandong Province, China.

Liran Zhang / Fengxiang Xie / Xinguo Wang / Dezhi Peng / Chunrui Bi / Lingbo Jiang / Dongman Zhao / Xinxin Tian / Debo Qi

PLoS ONE, Vol 12, Iss 6, p e

2017 Volume 0180618

Abstract: Currently, available data regarding previous cervical cytology and high-risk human papillomavirus (hrHPV) test results to detect invasive cervical cancer are limited and controversial in China. Therefore, this retrospective study in a population of ... ...

Abstract	Currently, available data regarding previous cervical cytology and high-risk human papillomavirus (hrHPV) test results to detect invasive cervical cancer are limited and controversial in China. Therefore, this retrospective study in a population of Chinese women with invasive cervical carcinoma aimed to gain further insight into the roles of cytology and hrHPV testing in cervical cancer screening.A total of 1214 cases with a histological diagnosis of invasive cervical cancer were retrieved from the Pathology Database of Jinan KingMed Diagnostics (JKD) over a 5-year period. Previous cytology and hrHPV test results of 469 patients carried out within the year before cancer diagnosis were documented.A higher percentage of patients who had undergone prior screening had micro-invasive cervical carcinoma than patients who had no prior screening (25.4% vs. 12.1%, P < 0.001). Of the 469 patients with available prior screening results, 170 had cytology alone, 161 had hrHPV testing alone, and 138 had both cytology and hrHPV testing. There was a significantly lower percentage of hrHPV-positive cases with adenocarcinoma than with squamous cell carcinoma (77.8% vs. 96.4%, P = 0.001). The hrHPV test showed a significantly higher sensitivity than cytology alone (94.4% vs. 85.3%, P = 0.006). The overall sensitivity of the combination of cytology and hrHPV testing (98.6%) was much higher than that of cytology alone (P < 0.001) but only marginally higher than that of hrHPV testing alone (P = 0.058).The results revealed that prior cervical screening can detect a significantly larger number of micro-invasive cervical cancers. The hrHPV test can provide a more sensitive and efficient strategy than cytology alone. As the addition of cytology to hrHPV testing can only marginally increase the efficiency of the hrHPV test, hrHPV testing should be used as the primary screening approach, especially in the low-resource settings of China.
Keywords	Medicine ; R ; Science ; Q
Subject code	610
Language	English
Publishing date	2017-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Nano Copper Induces Apoptosis in PK-15 Cells via a Mitochondria-Mediated Pathway

Zhang, Hui / Zhenyu Chang / Khalid Mehmood / Rao Zahid Abbas / Fazul Nabi / Mujeeb Ur Rehman / Xiaoxing Wu / Xinxin Tian / Xiaodan Yuan / Zhaoyang Li / Donghai Zhou

Biological trace element research. 2018 Jan., v. 181, no. 1

2018

Abstract: Nano-sized copper particles are widely used in various chemical, physical, and biological fields. However, earlier studies have shown that nano copper particles (40–100 μg/mL) can induce cell toxicity and apoptosis. Therefore, this study was conducted to ...

Abstract	Nano-sized copper particles are widely used in various chemical, physical, and biological fields. However, earlier studies have shown that nano copper particles (40–100 μg/mL) can induce cell toxicity and apoptosis. Therefore, this study was conducted to investigate the role of nano copper in mitochondrion-mediated apoptosis in PK-15 cells. The cells were treated with different doses of nano copper (20, 40, 60, and 80 μg/mL) to determine the effects of apoptosis using acridine orange/ethidium bromide (AO/EB) fluorescence staining and a flow cytometry assay. The levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in the PK-15 cells were examined using commercially available kits. Moreover, the mRNA levels of the Bax, Bid, Caspase-3, and CYCS genes were assessed by real-time PCR. The results revealed that nano copper exposure induced apoptosis and changed the mitochondrial membrane potential. In addition, nano copper significantly altered the levels of the Bax, Bid, Caspase-3, and CYCS genes at a concentration of 40 μg/mL. To summarize, nano copper significantly (P < 0.05) decreased the level of SOD and increased the level of MDA in PK-15 cells. Altogether, these results suggest that nano copper can play an important role in inducing the apoptotic pathway in PK-15 cells, which may be the mechanism by which nano copper induces nephrotoxicity.
Keywords	acridine orange ; analytical kits ; apoptosis ; caspase-3 ; copper ; copper nanoparticles ; cytotoxicity ; ethidium ; flow cytometry ; fluorescence ; genes ; malondialdehyde ; membrane potential ; messenger RNA ; mitochondrial membrane ; nephrotoxicity ; quantitative polymerase chain reaction ; staining ; superoxide dismutase
Language	English
Dates of publication	2018-01
Size	p. 62-70.
Publishing place	Springer US
Document type	Article
ZDB-ID	445336-0
ISSN	1559-0720 ; 0163-4984
ISSN (online)	1559-0720
ISSN	0163-4984
DOI	10.1007/s12011-017-1024-0
Database	NAL-Catalogue (AGRICOLA)

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