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Article ; Online: Methylene-Blue-Encapsulated Metal-Organic-Framework-Based Electrochemical POCT Platform for Multiple Detection of Heavy Metal Ions in Milk.

Xiong, Huiwen / Li, Pintao / Cun, Fei / Chen, Hui / Kong, Jilie

2023 Volume 13, Issue 8

Abstract: Considering the high risk of heavy metal ions (HMIs) transferring through the food chain and accumulating in milk, a flexible and facile point-of-care testing (POCT) platform is urgently needed for the accurate, sensitive, and highly selective on-site ... ...

Abstract	Considering the high risk of heavy metal ions (HMIs) transferring through the food chain and accumulating in milk, a flexible and facile point-of-care testing (POCT) platform is urgently needed for the accurate, sensitive, and highly selective on-site quantification of multiple HMIs in milk. In this work, a cost-effective disk with six screen-printed electrodes (SPEs) was designed for hand-held electrochemical detection. Metal organic frameworks (MOFs) were adopted to amplify and enhance the electrochemical signals of methylene blue (MB). Using differential pulse voltammetry (DPV) methods, low limits of detection for four HMIs (Cd
MeSH term(s)	Animals ; Metal-Organic Frameworks ; Methylene Blue ; Milk ; Metals, Heavy ; Point-of-Care Testing ; Ions
Chemical Substances	Metal-Organic Frameworks ; Methylene Blue (T42P99266K) ; Metals, Heavy ; Ions
Language	English
Publishing date	2023-08-02
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662125-3
ISSN	2079-6374 ; 2079-6374
ISSN (online)	2079-6374
ISSN	2079-6374
DOI	10.3390/bios13080783
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Innovative Highly Specific Nucleic Acid Isothermal Detection Assay Based on the Polymerization-Coupled Endonuclease Activity of Prokaryotic DNA Polymerase I

Li, Pintao / Lin, Qiuyuan / Xiong, Huiwen / Kong, Jilie / Ye, Xin / Fang, Xueen

Analytical Chemistry. 2023 Oct. 12, v. 95, no. 42 p.15755-15762

2023

Abstract: In this study, we developed an innovative highly specific nucleic acid isothermal detection assay based on prokaryotic DNA polymerase I with exquisitely designed fluorescent probes, achieving high sensitivity and 100% specificity within 30 min. The ... ...

Abstract	In this study, we developed an innovative highly specific nucleic acid isothermal detection assay based on prokaryotic DNA polymerase I with exquisitely designed fluorescent probes, achieving high sensitivity and 100% specificity within 30 min. The fluorescent nucleic acid probe was designed and constructed based on the specific flap cleavage endonuclease activity of prokaryotic DNA polymerase I (including the Bst, Bsu, Bsm, and Klenow DNA polymerases). The flap endonuclease activity depends on the length of the flap DNA and polymerization activity, which greatly reduces the false-positive rate caused by primer dimerization. This robust assay was also validated by the detection of rotavirus with great specificity and sensitivity. It could be a great alternative to qPCR in the field of point-of-care detection of pathogens.
Keywords	DNA ; DNA-directed DNA polymerase ; analytical chemistry ; dimerization ; fluorescence ; point-of-care systems ; polymerization
Language	English
Dates of publication	2023-1012
Size	p. 15755-15762.
Publishing place	American Chemical Society
Document type	Article ; Online
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.3c03261
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Article ; Online: Innovative Highly Specific Nucleic Acid Isothermal Detection Assay Based on the Polymerization-Coupled Endonuclease Activity of Prokaryotic DNA Polymerase I.

Li, Pintao / Lin, Qiuyuan / Xiong, Huiwen / Kong, Jilie / Ye, Xin / Fang, Xueen

Analytical chemistry

2023 Volume 95, Issue 42, Page(s) 15755–15762

Abstract	In this study, we developed an innovative highly specific nucleic acid isothermal detection assay based on prokaryotic DNA polymerase I with exquisitely designed fluorescent probes, achieving high sensitivity and 100% specificity within 30 min. The fluorescent nucleic acid probe was designed and constructed based on the specific flap cleavage endonuclease activity of prokaryotic DNA polymerase I (including the
MeSH term(s)	DNA Polymerase I ; Polymerization ; Nucleic Acid Amplification Techniques ; DNA ; Endonucleases ; Sensitivity and Specificity
Chemical Substances	DNA Polymerase I (EC 2.7.7.7) ; DNA (9007-49-2) ; Endonucleases (EC 3.1.-)
Language	English
Publishing date	2023-10-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.3c03261
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Article: Aptamer-Initiated Catalytic Hairpin Assembly Fluorescence Assay for Universal, Sensitive Exosome Detection

Zhou, Jiaqi / Lin, Qiuyuan / Huang, Zhipeng / Xiong, Huiwen / Yang, Bin / Chen, Hui / Kong, Jilie

Analytical chemistry. 2022 Apr. 04, v. 94, no. 15

2022

Abstract: Cancer-cell-derived exosomes are regarded as noninvasive biomarkers for early cancer diagnosis because of their critical roles in intercellular communication and molecular exchange. A robust aptamer-initiated catalytic hairpin assembly (AICHA) ... ...

Abstract	Cancer-cell-derived exosomes are regarded as noninvasive biomarkers for early cancer diagnosis because of their critical roles in intercellular communication and molecular exchange. A robust aptamer-initiated catalytic hairpin assembly (AICHA) fluorescence assay is proposed for universal, sensitive detection of cancer-derived exosomes. The AICHA was verified with the specific detection of MCF-7 cell-derived exosomes with a wide calibration range of 8.4 particles/μL to 8.4 × 10⁵ particles/μL and a low detection limit (LOD) of 0.5 particles/μL. The universality of the AICHA method was verified for PANC-1 cell-derived exosomes, the LOD of which was determined to be 0.1 particles/μL. The performances in serum samples were detected with a recovery rate range of 95.45–106.2%, which demonstrates its significant potential for protein biomarker analysis and cancer diagnosis.
Keywords	analytical chemistry ; biomarkers ; blood serum ; calibration ; cell communication ; detection limit ; exosomes ; fluorescence
Language	English
Dates of publication	2022-0404
Size	p. 5723-5728.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.2c00231
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Article: Electrolytic Corrosion Behavior of 20 Cu-20 Ni-54 NiFe

Tao, Yu-Qiang / Li, Zhi-You / Xiong, Hui-Wen / He, Min-Jie / Wang, Bing-Xin

Materials (Basel, Switzerland)

2022 Volume 15, Issue 15

Abstract: Here, 20 Cu-20 Ni-54 ... ...

Abstract	Here, 20 Cu-20 Ni-54 NiFe
Language	English
Publishing date	2022-08-04
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma15155377
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Article ; Online: Aptamer-Initiated Catalytic Hairpin Assembly Fluorescence Assay for Universal, Sensitive Exosome Detection.

Zhou, Jiaqi / Lin, Qiuyuan / Huang, Zhipeng / Xiong, Huiwen / Yang, Bin / Chen, Hui / Kong, Jilie

Analytical chemistry

2022 Volume 94, Issue 15, Page(s) 5723–5728

Abstract	Cancer-cell-derived exosomes are regarded as noninvasive biomarkers for early cancer diagnosis because of their critical roles in intercellular communication and molecular exchange. A robust aptamer-initiated catalytic hairpin assembly (AICHA) fluorescence assay is proposed for universal, sensitive detection of cancer-derived exosomes. The AICHA was verified with the specific detection of MCF-7 cell-derived exosomes with a wide calibration range of 8.4 particles/μL to 8.4 × 10
MeSH term(s)	Aptamers, Nucleotide/metabolism ; Biosensing Techniques/methods ; Exosomes/metabolism ; Fluorescence ; Humans ; Neoplasms/diagnosis
Chemical Substances	Aptamers, Nucleotide
Language	English
Publishing date	2022-04-04
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.2c00231
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Article: Recent progress of inert anodes for carbon-free aluminium electrolysis: a review and outlook

He, Yong / Zhou, Ke-chao / Zhang, Yan / Xiong, Hui-wen / Zhang, Lei

Journal of materials chemistry A. 2021 Nov. 23, v. 9, no. 45

2021

Abstract: Carbon-free aluminium electrolysis based on inert anodes is a disruptive and fundamental technology to reach the goal of carbon neutrality in the electrolytic aluminium industry, since it only releases O₂ instead of CO₂ derived from conventional ... ...

Abstract	Carbon-free aluminium electrolysis based on inert anodes is a disruptive and fundamental technology to reach the goal of carbon neutrality in the electrolytic aluminium industry, since it only releases O₂ instead of CO₂ derived from conventional aluminium electrolysis using carbon anodes. In recent years, the optimization of material preparation and advances in electrolysis progress have provided new opportunities for the industrial application of inert anodes. The research on inert anode materials has attracted considerable attention due to the big challenge of performance requirements under harsh working environment during electrolysis. Metals and cermets are the most likely alternative materials to carbon anodes, with satisfactory electrical conductivity, high thermal shock resistance and appropriate corrosion resistance. This review therefore introduces the development of inert anode materials and highlights their latest research progress in material design and electrolysis performance. The properties of different kinds of materials, particularly electrical conductivity and corrosion resistance, are compared systematically to provide a basis and reference for the selection and application of inert anode materials in the future. Finally, according to the latest advances in the industrial application of inert anodes, the development trends concerning electrolysis cells and process are discussed, with the aim of providing promising research directions for the inert anode technology.
Keywords	aluminum ; anodes ; carbon ; carbon dioxide ; corrosion ; electrical conductivity ; electrolysis ; industrial applications
Language	English
Dates of publication	2021-1123
Size	p. 25272-25285.
Publishing place	The Royal Society of Chemistry
Document type	Article
ZDB-ID	2702232-8
ISSN	2050-7496 ; 2050-7488
ISSN (online)	2050-7496
ISSN	2050-7488
DOI	10.1039/d1ta07198j
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Article ; Online: Electro-Optical Multiclassification Platform for Minimizing Occasional Inaccuracy in Point-of-Care Biomarker Detection.

Dai, Changhao / Xiong, Huiwen / He, Rui / Zhu, Chenxin / Li, Pintao / Guo, Mingquan / Gou, Jian / Mei, Miaomiao / Kong, Derong / Li, Qiang / Wee, Andrew Thye Shen / Fang, Xueen / Kong, Jilie / Liu, Yunqi / Wei, Dacheng

Advanced materials (Deerfield Beach, Fla.)

2024 Volume 36, Issue 15, Page(s) e2312540

Abstract: On-site diagnostic tests that accurately identify disease biomarkers lay the foundation for self-healthcare applications. However, these tests routinely rely on single-mode signals and suffer from insufficient accuracy, especially for multiplexed point- ... ...

Abstract	On-site diagnostic tests that accurately identify disease biomarkers lay the foundation for self-healthcare applications. However, these tests routinely rely on single-mode signals and suffer from insufficient accuracy, especially for multiplexed point-of-care tests (POCTs) within a few minutes. Here, this work develops a dual-mode multiclassification diagnostic platform that integrates an electrochemiluminescence sensor and a field-effect transistor sensor in a microfluidic chip. The microfluidic channel guides the testing samples to flow across electro-optical sensor units, which produce dual-mode readouts by detecting infectious biomarkers of tuberculosis (TB), human rhinovirus (HRV), and group B streptococcus (GBS). Then, machine-learning classifiers generate three-dimensional (3D) hyperplanes to diagnose different diseases. Dual-mode readouts derived from distinct mechanisms enhance the anti-interference ability physically, and machine-learning-aided diagnosis in high-dimensional space reduces the occasional inaccuracy mathematically. Clinical validation studies with 501 unprocessed samples indicate that the platform has an accuracy approaching 99%, higher than the 77%-93% accuracy of rapid point-of-care testing technologies at 100% statistical power (>150 clinical tests). Moreover, the diagnosis time is 5 min without a trade-off of accuracy. This work solves the occasional inaccuracy issue of rapid on-site diagnosis, endowing POCT systems with the same accuracy as laboratory tests and holding unique prospects for complicated scenes of personalized healthcare.
MeSH term(s)	Humans ; Point-of-Care Systems ; Point-of-Care Testing ; Microfluidics ; Biomarkers
Chemical Substances	Biomarkers
Language	English
Publishing date	2024-02-04
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1474949-X
ISSN	1521-4095 ; 0935-9648
ISSN (online)	1521-4095
ISSN	0935-9648
DOI	10.1002/adma.202312540
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Article ; Online: Efficient Microfluidic-Based Air Sampling/Monitoring Platform for Detection of Aerosol SARS-CoV-2 On-site.

Xiong, Huiwen / Ye, Xin / Li, Yang / Qi, Jun / Fang, Xueen / Kong, Jilie

Analytical chemistry

2021 Volume 93, Issue 9, Page(s) 4270–4276

Abstract: Airborne pathogens have been considered as highly infectious and transmittable between humans. With the pandemic outbreak of the coronavirus disease 2019 (COVID-19), an on-site diagnostic system-integrated airborne pathogen-monitoring machine is ... ...

Abstract	Airborne pathogens have been considered as highly infectious and transmittable between humans. With the pandemic outbreak of the coronavirus disease 2019 (COVID-19), an on-site diagnostic system-integrated airborne pathogen-monitoring machine is recommended by experts for preventing and controlling the early stage β-coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spread. In this work, a small-volume rotating microfluidic fluorescence chip-integrated aerosol SARS-CoV-2 sampling system was constructed to satisfy the demand for rapid on-site sample collection and detection of SARS-CoV-2. The rotating microfluidic fluorescence system with small volume has very high sensitivity in the detection of SARS-CoV-2 (detection limit of 10 copies/μL with the shortest Ct value of 15 min), which is comparable to reverse transcription polymerase chain reaction (RT-PCR). The precision variation coefficients within/between batches are very low [coefficient of variation (CV) % ≤ 5.0%]. Our work has passed the comprehensive inspection of the microfluidic chip performance by the Shanghai Medical Device Testing Institute [National Medical Inspection (Design) no. 4408] and successfully tested 115 clinical samples. The integrated system exhibits 100% specificity, high sensitivity (10 copies/μL), and good precision (CV % ≤ 5.0%) in the rapid detection of SARS-CoV-2, thus realizing rapid monitoring and diagnostics of SARS-CoV-2 nucleic acid on-site.
MeSH term(s)	Aerosols/analysis ; Air ; COVID-19/diagnosis ; COVID-19/virology ; COVID-19 Testing/instrumentation ; COVID-19 Testing/methods ; Fluorescence ; Humans ; Lab-On-A-Chip Devices ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; Sensitivity and Specificity
Chemical Substances	Aerosols
Language	English
Publishing date	2021-02-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.0c05154
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Article: Rapid Differential Diagnosis of Seven Human Respiratory Coronaviruses Based on Centrifugal Microfluidic Nucleic Acid Assay

Xiong, Huiwen / Ye, Xin / Li, Yang / Wang, Lijuan / Zhang, Jin / Fang, Xueen / Kong, Jilie

Analytical chemistry. 2020 Oct. 19, v. 92, no. 21

2020

Abstract: With the global outbreak of the coronavirus disease 2019 (COVID-19), the highly infective, highly pathogenic, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has attracted great attention. Currently, a method to simultaneously diagnose the ... ...

Abstract	With the global outbreak of the coronavirus disease 2019 (COVID-19), the highly infective, highly pathogenic, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has attracted great attention. Currently, a method to simultaneously diagnose the seven known types human coronaviruses remains lacking and is urgently needed. In this work, we successfully developed a portable microfluidic system for the rapid, accurate, and simultaneous detection of SARS-CoV, middle east respiratory syndrome coronavirus (MERS-CoV), SARS-CoV-2, and four other human coronaviruses (HCoVs) including HCoV-229E, HCoV-OC43, HCoV-NL63, and HCoV-HKU1. The disk-like microfluidic platform integrated with loop-mediated isothermal amplification provides highly accurate, sensitive, and specific results with a wide linear range within 40 min. The diagnostic tool achieved 100% consistency with the “gold standard” polymerase chain reaction in detecting 54 real clinical samples. The integrated system, with its simplicity, is urgently needed for the diagnosis of SARS-CoV-2 during the COVID-19 pandemic.
Keywords	Betacoronavirus 1 ; COVID-19 infection ; Middle East respiratory syndrome coronavirus ; Severe acute respiratory syndrome coronavirus 2 ; analytical chemistry ; diagnostic techniques ; humans ; loop-mediated isothermal amplification ; nucleic acids ; polymerase chain reaction
Language	English
Dates of publication	2020-1019
Size	p. 14297-14302.
Publishing place	American Chemical Society
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.0c03364
Database	NAL-Catalogue (AGRICOLA)

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