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  1. Article: CircRNA mmu_circ_0000021 regulates microvascular function via the miR-143-3p/NPY axis and intracellular calcium following ischemia/reperfusion injury.

    Xiong, Jingjie / Hu, Yisen / Liu, Yi / Zeng, Xiaocong

    Cell death discovery

    2022  Volume 8, Issue 1, Page(s) 315

    Abstract: Cardiac ischemia-reperfusion (I/R) is associated with a high rate of complications. Restoring microvascular function is crucial for cardiac repair. However, the molecular mechanisms by which the circRNAs repairs microvascular dysfunction are unknown. ... ...

    Abstract Cardiac ischemia-reperfusion (I/R) is associated with a high rate of complications. Restoring microvascular function is crucial for cardiac repair. However, the molecular mechanisms by which the circRNAs repairs microvascular dysfunction are unknown. High-throughput RNA sequencing and quantitative real-time PCR (qRT-PCR) were used to measures circRNA levels in cardiac tissue samples. We found a total of 80 up-regulated and 54 down-regulated differentially expressed circRNAs, of which mmu_circ_0000021 were consistent with bioinformatics predictions. Next, mmu_circ_0000021 knockdown and overexpression were performed to indicate the functional role of mmu_circ_0000021. The interaction of mmu_circ_0000021, miR-143-3p and NPY were evaluated using dual-luciferase assays, RNA pull-down assays and RNA immunoprecipitation (RIP). Immunohistochemistry, transmission electron microscopy, and immunofluorescence were used to determine the presence of leukocytes and changes in microvascular morphology and function. Mechanistically, mmu_circ_0000021 involved in regulating microvascular dysfunction via miR-143-3p by targeting NPY. However, the contraction of microvascular spasm caused by NPY is related to calmodulin. By regulating NPY, Circular RNA (circRNA) further affects microvascular spasm, regulates microcirculation disorders, and restores cardiac function. Our findings highlight a novel role for mmu_circ_0000021 by regulating microvascular function following I/R injury.
    Language English
    Publishing date 2022-07-11
    Publishing country United States
    Document type Journal Article
    ISSN 2058-7716
    ISSN 2058-7716
    DOI 10.1038/s41420-022-01108-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Overexpression of Activating Transcription Factor 3 Alleviates Cardiac Microvascular Ischemia/Reperfusion Injury in Rats.

    Liu, Yi / Hu, Yisen / Xiong, Jingjie / Zeng, Xiaocong

    Frontiers in pharmacology

    2021  Volume 12, Page(s) 598959

    Abstract: Activating transcription factor 3 (ATF3) has been confirmed to be responsive to oxidative stress and to negatively regulate the activity of Toll-like receptor 4 (TLR4). However, the effect of ATF3 on cardiac microvascular ischemia/reperfusion (I/R) ... ...

    Abstract Activating transcription factor 3 (ATF3) has been confirmed to be responsive to oxidative stress and to negatively regulate the activity of Toll-like receptor 4 (TLR4). However, the effect of ATF3 on cardiac microvascular ischemia/reperfusion (I/R) injury remains unknown. The GEO2R online tool was employed to obtain differentially expressed genes GSE4105 and GSE122020, in two rat I/R injury microarray datasets. We established a rat myocardial I/R model
    Language English
    Publishing date 2021-02-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2021.598959
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Identification of a 5-Nutrient Stress-Sensitive Gene Signature to Predict Survival for Colorectal Cancer.

    Xiong, Jingjie / Xiong, Subing / Feng, Xi / Zhang, Huaming / Su, Qisheng

    publication RETRACTED

    BioMed research international

    2022  Volume 2022, Page(s) 2587120

    Abstract: Background: The high heterogeneity and the complexity of the tumor microenvironment of colorectal cancer (CRC) have enhanced the difficulty of prognosis prediction based on conventional clinical indicators. Recent studies revealed that tumor cells could ...

    Abstract Background: The high heterogeneity and the complexity of the tumor microenvironment of colorectal cancer (CRC) have enhanced the difficulty of prognosis prediction based on conventional clinical indicators. Recent studies revealed that tumor cells could overcome various nutritional deficiencies by gene regulation and metabolic remodeling. However, whether differentially expressed genes (DEGs) in CRC cells under kinds of nutrient deficiency could be used to predict prognosis remained unveiled.
    Methods: Three datasets (GSE70976, GSE13548, and GSE116087), in which colon cancer cells were, respectively, cultured in serum-free, glucose-free, or glutamine-free medium, were included to delineate the profiles of gene expression by nutrient stress. DEGs were figured out in three datasets, and gene functional analysis was performed. Survival analyses and Cox proportional hazards model were then used to identify nutrient stress sensitive genes in CRC datasets (GSE39582 and TCGA COAD). Then, a 5-gene signature was constructed and the risk scores were also calculated. Survival analyses, cox analyses, and nomogram were applied to predict the prognosis of patients with colorectal cancer. The effectiveness of the risk model was also tested.
    Results: A total of 48 genes were found to be dysregulated in serum, glucose, or glutamine-deprived CRC cells, which were mainly enriched in cell cycle and endoplasmic reticulum stress pathways. After further analyses, 5 genes, MCM5, MCM6, CDCA2, GINS2, and SPC25, were identified to be differentially expressed in CRC and be related to prognosis of in CRC datasets. We used the above nutrient stress-sensitive genes to construct a risk scoring model. CRC samples in the datasets were divided into low-risk and high-risk groups. Data showed that higher risk scores were associated with better outcomes and risk scores decreased significantly with tumor procession. Moreover, the risk score could be used to predict the probability of survival based on nomogram.
    Conclusions: The 5-nutrient stress-sensitive gene signature could act as an independent biomarker for survival prediction of CRC patients.
    MeSH term(s) Biomarkers, Tumor/genetics ; Biomarkers, Tumor/metabolism ; Chromosomal Proteins, Non-Histone/genetics ; Colorectal Neoplasms/pathology ; Gene Expression Regulation, Neoplastic ; Humans ; Nutrients ; Prognosis ; Tumor Microenvironment
    Chemical Substances Biomarkers, Tumor ; Chromosomal Proteins, Non-Histone ; GINS2 protein, human
    Language English
    Publishing date 2022-04-18
    Publishing country United States
    Document type Journal Article ; Retracted Publication
    ZDB-ID 2698540-8
    ISSN 2314-6141 ; 2314-6133
    ISSN (online) 2314-6141
    ISSN 2314-6133
    DOI 10.1155/2022/2587120
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: MiR-98-5p promotes ischemia/reperfusion-induced microvascular dysfunction by targeting NGF and is a potential biomarker for microvascular reperfusion.

    Hu, Yisen / Xiong, Jingjie / Wen, Hong / Wei, Heng / Zeng, Xiaocong

    Microcirculation (New York, N.Y. : 1994)

    2020  Volume 28, Issue 1, Page(s) e12657

    Abstract: Objective: This study examined the correlation between serum miR-98-5p levels and indices of microvascular reperfusion in patients undergoing primary percutaneous coronary intervention (pPCI) after ST-segment elevation myocardial infarction (STEMI). ... ...

    Abstract Objective: This study examined the correlation between serum miR-98-5p levels and indices of microvascular reperfusion in patients undergoing primary percutaneous coronary intervention (pPCI) after ST-segment elevation myocardial infarction (STEMI). Additionally, we evaluated the mechanisms by which miR-98-5p promoted ischemia/reperfusion (I/R)-induced injury in both cultured cell lines and an animal model.
    Methods: Circulating miR-98-5p levels were measured and compared from 171 STEMI patients undergoing pPCI, who were divided into two groups: no-reflow and reflow. The levels of miR-98-5p, nerve growth factor (NGF), and transient receptor potential vanilloid 1 (TRPV1) were analyzed in cultured human coronary endothelial cells (HCECs) exposed to hypoxia/reoxygenation (H/R). The effects of antagomir-98-5p on myocardial I/R-induced microvascular dysfunction in vivo were evaluated. Target gene expression and activity were assessed.
    Results: Higher miR-98-5p levels were associated with compromised indices of microvascular reperfusion. In vitro experiments on HCECs showed that exposure to H/R significantly increased miR-98-5p levels. We identified NGF as a novel target of miR-98-5p. Further, antagomir-98-5p relieved microvascular dysfunction and enhanced the expression of NGF and TRPV1 in the rat myocardial I/R model.
    Conclusions: MiR-98-5p promotes microvascular dysfunction by targeting the NGF-TRPV1 axis. Serum miR-98-5p serves as a potential biomarker for microvascular reperfusion.
    MeSH term(s) Aged ; Biomarkers/blood ; Cells, Cultured ; Coronary Vessels/metabolism ; Coronary Vessels/pathology ; Endothelial Cells/metabolism ; Endothelial Cells/physiology ; Female ; Follow-Up Studies ; Gene Expression Regulation ; Humans ; Male ; MicroRNAs/blood ; Microvessels/metabolism ; Microvessels/pathology ; Middle Aged ; Myocardial Reperfusion Injury/blood ; Myocardial Reperfusion Injury/pathology ; Nerve Growth Factor/blood
    Chemical Substances Biomarkers ; MIRN98 microRNA, human ; MicroRNAs ; NGF protein, human ; Nerve Growth Factor (9061-61-4)
    Language English
    Publishing date 2020-09-28
    Publishing country United States
    Document type Clinical Trial ; Journal Article ; Observational Study ; Research Support, Non-U.S. Gov't
    ZDB-ID 1217758-1
    ISSN 1549-8719 ; 1073-9688
    ISSN (online) 1549-8719
    ISSN 1073-9688
    DOI 10.1111/micc.12657
    Database MEDical Literature Analysis and Retrieval System OnLINE

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