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  1. Article ; Online: Insight into the Systematics of Novel Entomopathogenic Fungi Associated with Armored Scale Insect, Kuwanaspis howardi (Hemiptera

    Xiu-Lan Xu / Qian Zeng / Yi-Cong Lv / Rajesh Jeewon / Sajeewa S. N. Maharachchikumbura / Dhanushka N. Wanasinghe / Kevin D. Hyde / Qian-Gang Xiao / Ying-Gao Liu / Chun-Lin Yang

    Journal of Fungi, Vol 7, Iss 628, p

    Diaspididae) in China

    2021  Volume 628

    Abstract: This study led to the discovery of three entomopathogenic fungi associated with Kuwanaspis howardi , a scale insect on Phyllostachys heteroclada (fishscale bamboo) and Pleioblastus amarus (bitter bamboo) in China. Two of these species belong to ... ...

    Abstract This study led to the discovery of three entomopathogenic fungi associated with Kuwanaspis howardi , a scale insect on Phyllostachys heteroclada (fishscale bamboo) and Pleioblastus amarus (bitter bamboo) in China. Two of these species belong to Podonectria : P. kuwanaspidis X.L. Xu & C.L. Yang sp. nov. and P . novae-zelandiae Dingley. The new species P. kuwanaspidis has wider and thicker setae, longer and wider asci, longer ascospores, and more septa as compared with similar Podonectria species. The morphs of extant species P. novae-zelandiae is confirmed based on sexual and asexual morphologies. Maximum likelihood and Bayesian inference analyses of ITS, LSU, SSU, tef1-α , and rpb2 sequence data provide further evidence for the validity of the two species and their placement in Podonectriaceae (Pleosporales). The second new species, Microcera kuwanaspidis X.L. Xu & C.L. Yang sp. nov., is established based on DNA sequence data from ITS, LSU, SSU, tef1-α , rpb1 , rpb2 , acl1 , act , cmdA , and his3 gene regions, and it is characterized by morphological differences in septum numbers and single conidial mass.
    Keywords 2 new taxa ; bamboo ; entomopathogens ; Nectriaceae ; Podonectriaceae ; scale insect ; Biology (General) ; QH301-705.5
    Subject code 590
    Language English
    Publishing date 2021-08-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Application of droplet digital PCR in detection of seed-transmitted pathogen Acidovorax citrulli

    Yu LU / Hai-jun ZHANG / Zi-jing ZHAO / Chang-long WEN / Ping WU / Shun-hua SONG / Shuan-cang YU / Lai-xin LUO / Xiu-lan XU

    Journal of Integrative Agriculture, Vol 19, Iss 2, Pp 561-

    2020  Volume 569

    Abstract: Bacterial fruit blotch caused by Acidovorax citrulli is a serious threat to cucurbit industry worldwide. The pathogen is seed-transmitted, so seed detection to prevent distribution of contaminated seed is crucial in disease management. In this study, we ... ...

    Abstract Bacterial fruit blotch caused by Acidovorax citrulli is a serious threat to cucurbit industry worldwide. The pathogen is seed-transmitted, so seed detection to prevent distribution of contaminated seed is crucial in disease management. In this study, we adapted a quantitative real-time PCR (qPCR) assay to droplet digital PCR (ddPCR) format for A. citrulli detection by optimizing reaction conditions. The performance of ddPCR in detecting A. citrulli pure culture, DNA, infested watermelon/melon seed and commercial seed samples were compared with multiplex PCR, qPCR, and dilution plating method. The lowest concentrations detected (LCD) by ddPCR reached up to 2 fg DNA, and 102 CFU mL−1 bacterial cells, which were ten times more sensitive than those of the qPCR. When testing artificially infested watermelon and melon seed, 0.1% infestation level was detectable using ddPCR and dilution plating method. The 26 positive samples were identified in 201 commercial seed samples through ddPCR, which was the highest positive number among all the methods. High detection sensitivity achieved by ddPCR demonstrated a promising technique for improving seed-transmitted pathogen detection threshold in the future.
    Keywords bacterial fruit blotch ; Acidovorax citrulli ; droplet digital PCR ; seed detection ; quantitative real-time PCR ; Agriculture (General) ; S1-972
    Language English
    Publishing date 2020-02-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article: Identification of aged bloodstains through mRNA profiling: Experiments results on selected markers of 30- and 50-year-old samples

    Zhao, Hemiao / Chong Wang / Lan Hu / Lan Yao / Qingluan Lin / Wanshui Li / XiuLan Xu

    Forensic science international. 2017 Mar., v. 272

    2017  

    Abstract: Remarkable progress has been made in recent years on the research of body fluid identification through messenger RNA(mRNA) profiling. In order to examine the viability of mRNA profiling as a method to identify aged bloodstains, this study tested two ... ...

    Abstract Remarkable progress has been made in recent years on the research of body fluid identification through messenger RNA(mRNA) profiling. In order to examine the viability of mRNA profiling as a method to identify aged bloodstains, this study tested two groups of bloodstain samples, dated 30 years and 50 years back respectively, on seven blood specific markers, i.e. HBB, HBA, GYPA, CD93, ALAS2, SPTB (91bp and 247bp primers), and PBGD. Test results indicate that HBA and HBB are the most stable markers in aged bloodstains, returning positive results in over 80% of the 50-year-old samples and over 90% of the 30-year-old samples. This finding proves mRNA profiling an effective way of identifying aged bloodstains.
    Keywords blood ; messenger RNA ; viability
    Language English
    Dates of publication 2017-03
    Size p. e1-e6.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 424042-x
    ISSN 1872-6283 ; 0379-0738
    ISSN (online) 1872-6283
    ISSN 0379-0738
    DOI 10.1016/j.forsciint.2017.01.006
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: DNA profiling of spermatozoa by laser capture microdissection and low volume-PCR.

    Cai-xia Li / Jun-ping Han / Wen-yan Ren / An-quan Ji / Xiu-lan Xu / Lan Hu

    PLoS ONE, Vol 6, Iss 8, p e

    2011  Volume 22316

    Abstract: Genetic profiling of sperm from complex biological mixtures such as sexual assault casework samples requires isolation of a pure sperm population and the ability to analyze low abundant samples. Current standard procedure for sperm isolation includes ... ...

    Abstract Genetic profiling of sperm from complex biological mixtures such as sexual assault casework samples requires isolation of a pure sperm population and the ability to analyze low abundant samples. Current standard procedure for sperm isolation includes preferential lysis of epithelial contaminants followed by collection of intact sperm by centrifugation. While effective for samples where sperm are abundant, this method is less effective when samples contain few spermatozoa. Laser capture microdissection (LCM) is a proven method for the isolation of cells biological mixtures, even when found in low abundance. Here, we demonstrate the efficacy of LCM coupled with on-chip low volume PCR (LV-PCR) for the isolation and genotyping of low abundance sperm samples. Our results indicate that this method can obtain complete profiles (13-16 loci) from as few as 15 sperm cells with 80% reproducibility, whereas at least 40 sperm cells are required to profile 13-16 loci by standard 'in-tube' PCR. Further, LCM and LV-PCR of a sexual assault casework sample generated a DNA genotype that was consistent with that of the suspect. This method was unable, however, to analyze a casework sample from a gang rape case in which two or more sperm contributors were in a mixed population. The results indicate that LCM and LV-PCR is sensitive and effective for genotyping sperm from sperm/epithelial cell mixtures when epithelial lysis may be insufficient due to low abundance of sperm; LCM and LV-PCR, however, failed in a casework sample when spermatozoa from multiple donors was present, indicating that further study is necessitated.
    Keywords Medicine ; R ; Science ; Q
    Subject code 630
    Language English
    Publishing date 2011-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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