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Article ; Online: Dnmt3b ablation affects fracture repair process by regulating apoptosis.

Wang, Xu / Ge, Qinwen / Zeng, Qinghe / Zou, Kaiao / Bao, Zhengsheng / Ying, Jun / Wu, Zhen / Jin, Hongting / Chen, Jiali / Xu, Taotao

BMC musculoskeletal disorders

2024 Volume 25, Issue 1, Page(s) 180

Abstract: Purpose: Previous studies have shown that DNA methyltransferase 3b (Dnmt3b) is the only Dnmt responsive to fracture repair and Dnmt3b ablation in Prx1-positive stem cells and chondrocyte cells both delayed fracture repair. Our study aims to explore the ... ...

Abstract	Purpose: Previous studies have shown that DNA methyltransferase 3b (Dnmt3b) is the only Dnmt responsive to fracture repair and Dnmt3b ablation in Prx1-positive stem cells and chondrocyte cells both delayed fracture repair. Our study aims to explore the influence of Dnmt3b ablation in Gli1-positive stem cells in fracture healing mice and the underlying mechanism. Methods: We generated Gli1-CreERT2; Dnmt3bflox/flox (Dnmt3b Results: The cartilaginous callus significantly decrease in ablation of Dnmt3b in Gli1-positive stem cells during fracture repair. The chondrogenic and osteogenic indicators (Sox9 and Runx2) in the fracture healing tissues in Dnmt3b Conclusions: Our study provides an evidence that Dnmt3b ablation Gli1-positive stem cells can affect fracture healing and lead to poor fracture healing by regulating apoptosis to decrease chondrocyte hypertrophic maturation.
MeSH term(s)	Animals ; Mice ; Apoptosis ; Bony Callus/pathology ; Fracture Healing/physiology ; Tibial Fractures/surgery ; Zinc Finger Protein GLI1
Chemical Substances	Zinc Finger Protein GLI1 ; Dnmt3b protein, mouse (EC 2.1.1.-)
Language	English
Publishing date	2024-02-27
Publishing country	England
Document type	Journal Article
ZDB-ID	2041355-5
ISSN	1471-2474 ; 1471-2474
ISSN (online)	1471-2474
ISSN	1471-2474
DOI	10.1186/s12891-024-07283-7
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Article ; Online: Closed reduction and plaster immobilization: an alternative solution for patients with developmental dysplasia of the hip who failed Pavlik harness treatment.

Zhou, Ping / Zhang, Jianqiang / Dan, Tangzi / Xu, Taotao / Kang, Xiaopeng / Hang, Yong / Zhou, You

ANZ journal of surgery

2023 Volume 93, Issue 3, Page(s) 663–668

Abstract: Background: The current study aims to investigate the clinical efficacy of closed reduction and cast immobilization for patients with developmental dysplasia of the hip (DDH) who failed Pavlik harness treatment.: Methods: Patients with DDH who ... ...

Abstract	Background: The current study aims to investigate the clinical efficacy of closed reduction and cast immobilization for patients with developmental dysplasia of the hip (DDH) who failed Pavlik harness treatment. Methods: Patients with DDH who underwent cast immobilization under general anaesthesia after the failure of the Pavlik harness or Tübingen brace treatment between January 2015 and December 2020 were retrospectively recruited. General information, including Graf classification of initial diagnosis, initial treatment, age of cast immobilization, IHDI classification, AI index, avascular necrosis (AVN), and residual dysplasia, was collected. The incidence of AVN and late acetabular dysplasia (LACD) was also estimated. Moreover, factors related to AVN and LACD were investigated by multiple logistic regression analysis. Results: Thirty-four patients (47 hips) were finally included in the current study. Of these patients, 31 hips (66.0%) were successfully treated with closed reduction and cast immobilization. Open reduction was successfully performed in 16 hips (34.0%). Till the latest follow-up, LACD and AVN were found in 13 (27.7%) and 10 (21.3%) hips, respectively. In the open reduction group, type III and IV of the IHDI classification and type IV of the Ultrasound Graf classification were significantly higher when compared with the closed reduction group. Multiple logistic regression showed that failure of closed reduction was related to the initial types of the Ultrasound Graf and IHDI classifications. Conclusions: Although the success rate of closed reduction after early harness failure in DDH is only 66%, we still advocate closed reduction as a first-line treatment for children who have failed sling treatment. Even if closed reduction fails, open reduction can still achieve acceptable results.
MeSH term(s)	Child ; Humans ; Infant ; Hip Dislocation, Congenital/diagnostic imaging ; Hip Dislocation, Congenital/therapy ; Hip Dislocation, Congenital/complications ; Retrospective Studies ; Developmental Dysplasia of the Hip/complications ; Treatment Outcome ; Femur Head Necrosis
Language	English
Publishing date	2023-02-02
Publishing country	Australia
Document type	Journal Article
ZDB-ID	2050749-5
ISSN	1445-2197 ; 1445-1433 ; 0004-8682
ISSN (online)	1445-2197
ISSN	1445-1433 ; 0004-8682
DOI	10.1111/ans.18285
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Zs.A 180: Show issues

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Article ; Online: Network pharmacology-based pharmacological mechanism prediction on Eucommia ulmoides against rheumatoid arthritis.

Ying, Yonggan / Tang, Zhaopeng / Niu, Feng / Xu, Taotao / Xia, Chenjie / Zhang, Shuijun

Medicine

2022 Volume 101, Issue 29, Page(s) e29658

Abstract: Rheumatoid arthritis (RA) is a common chronic autoimmune disease characterized by synovial inflammation and progressive joint destruction. Eucommia ulmoides (EU) is a kidney-tonifying Chinese medicine that has been applied to treat RA for decides. The ... ...

Abstract	Rheumatoid arthritis (RA) is a common chronic autoimmune disease characterized by synovial inflammation and progressive joint destruction. Eucommia ulmoides (EU) is a kidney-tonifying Chinese medicine that has been applied to treat RA for decides. The present study aims to explore pharmacological mechanisms of EU against RA using network pharmacology approach. Traditional Chinese Medicine Systems Pharmacology (TCMSP) database was used to screen active ingredients of EU, and their relative targets were fished from UniProt database. RA-related targets were screened from GeneCards database and DisGeNET database. The overlapping genes between EU and RA were identified by Venn diagram, and further analyzed for protein-protein interaction (PPI), Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG). Fifty active ingredients were identified in EU, and corresponded to 207 targets. Meanwhile, 499 targets were closely associated with RA development. A total of 50 overlapping genes between EU and RA were identified, which were regarded as therapeutically relevant. GO enrichment analysis indicated that EU exerted antiRA effects depending on regulating multiple biological processes including inflammatory response, oxidative stress, cell apoptosis and matrix catabolism. Several key pathways such as TNF pathway, IL-17 pathway, T cell receptor pathway, NOD-like receptor pathway and Toll-like receptor pathway, were involved in the above biological processes. Network pharmacology revealed that EU exerts therapeutic effects on RA through multi-ingredients, multi-targets and multi-pathways, which provides basis for its clinical application and promising directions for subsequent research.
MeSH term(s)	Arthritis, Rheumatoid/drug therapy ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Eucommiaceae ; Humans ; Medicine, Chinese Traditional ; Network Pharmacology
Chemical Substances	Drugs, Chinese Herbal
Language	English
Publishing date	2022-07-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	80184-7
ISSN	1536-5964 ; 0025-7974
ISSN (online)	1536-5964
ISSN	0025-7974
DOI	10.1097/MD.0000000000029658
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Article: LIMS2

Su, Chenying / Cai, Xiaona / Xu, Taotao / Wu, Yungang / Wang, Licong / Chen, Pinjie / Su, Chenxian

Journal of oncology

2022 Volume 2022, Page(s) 4811260

Abstract: Methods: GEO, GEPIA, and UALCAN databases were used to assess : Results: LIMS2: Conclusion: ... ...

Abstract	Methods: GEO, GEPIA, and UALCAN databases were used to assess Results: LIMS2 Conclusion: LIMS2
Language	English
Publishing date	2022-10-13
Publishing country	Egypt
Document type	Journal Article
ZDB-ID	2461349-6
ISSN	1687-8469 ; 1687-8450
ISSN (online)	1687-8469
ISSN	1687-8450
DOI	10.1155/2022/4811260
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Article ; Online: The association between dietary inflammation index and the risk of rheumatoid arthritis in Americans.

Xiang, Shate / Wang, Yao / Qian, Suhai / Li, Jie / Jin, Yibo / Ding, Xinghong / Xu, Taotao

Clinical rheumatology

2022 Volume 41, Issue 9, Page(s) 2647–2658

Abstract: Background: The correlation between dietary inflammation index (DII) and rheumatoid arthritis (RA) has been found, but the effect of confounding factors is not considered. This study aims to further explore the association between DII and RA risk by ... ...

Abstract	Background: The correlation between dietary inflammation index (DII) and rheumatoid arthritis (RA) has been found, but the effect of confounding factors is not considered. This study aims to further explore the association between DII and RA risk by taking the Americans as the research object. Methods: The data from the 2005-2018 National Health and Nutrition Examination Survey (NHANES) database included 1819 self-reported RA individuals and 8602 non-RA individuals. The analytical methods include logistic regression, additive model, smooth curve fitting, and the recursive algorithm. Results: There was a positive correlation between DII and RA in Americans (β = 1.068, 95% CI = 1.026 to 1.111, P = 0.001). This result was still presented in the subgroup analysis, including age less than 50 years, female, other Hispanics, BMI ≥ 25, and federal poverty rate > 185%, and it was more pronounced in smokers. The results show that the superposition of DII and other risk factors would increase the risk of RA (β > 1.068). In addition, individuals with RA are inadequate in intake of anti-inflammatory foods, in line with the Mediterranean diet. Conclusions: The inflammatory potential of the diet is positively correlated with the risk of RA, and has a superimposed effect with other risk factors, increasing the probability of the risk of disease. These results emphasize that reducing the intake of pro-inflammatory foods may be an effective measure to prevent the onset of rheumatoid arthritis. However, eating anti-inflammatory foods exclusively is not the best option. Intaking some pro-inflammatory foods like protein, energy, and total saturated acids may be necessary to maintain the physiological function of the human body. Key Points • Dietary inflammation index (DII) is positively correlated with RA risk. • When DII and other risk factors appear at the same time, the effects of the two will be superimposed on each other, increasing the risk of RA. • When the DII is the same, Hispanic has a higher incidence of RA. • Among the pro-inflammatory foods, the intake of protein, energy, and saturated fatty acids is still required by RA patients.
MeSH term(s)	Arthritis, Rheumatoid/epidemiology ; Diet/adverse effects ; Diet, Mediterranean ; Female ; Humans ; Inflammation/diagnosis ; Middle Aged ; Nutrition Surveys ; Risk Factors
Language	English
Publishing date	2022-05-28
Publishing country	Germany
Document type	Journal Article
ZDB-ID	604755-5
ISSN	1434-9949 ; 0770-3198
ISSN (online)	1434-9949
ISSN	0770-3198
DOI	10.1007/s10067-022-06217-9
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Zs.A 1740: Show issues

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Article: [Advances on pentraxin 3 in osteoporosis and fracture healing].

Lu, Jia-Jun / Sun, Yan / Zhang, Xuan / Wang, Qiao-Qi / Xiang, Zhou-Yi / Ling, Yi-Qing / Tong, Pei-Jian / Xu, Tao-Tao

Zhongguo gu shang = China journal of orthopaedics and traumatology

2023 Volume 36, Issue 4, Page(s) 393–398

Abstract: Pentaxin 3 (PTX3), as a multifunctional glycoprotein, plays an important role in regulating inflammatory response, promoting tissue repair, inducing ectopic calcification and maintaining bone homeostasis. The effect of PTX3 on bone mineral density (BMD) ... ...

Abstract	Pentaxin 3 (PTX3), as a multifunctional glycoprotein, plays an important role in regulating inflammatory response, promoting tissue repair, inducing ectopic calcification and maintaining bone homeostasis. The effect of PTX3 on bone mineral density (BMD) may be affected by many factors. In PTX3 knockout mice and osteoporosis (OP) patients, the deletion of PTX3 will lead to decrease of BMD. In Korean community "Dong-gu study", it was found that plasma PTX3 was negatively correlated with BMD of femoral neck in male elderly patients. In terms of bone related cells, PTX3 plays an important role in maintaining the phenotype and function of osteoblasts (OB) in OP state;for osteoclast (OC), PTX3 in inflammatory state could stimulate nuclear factor κ receptor activator of nuclear factor-κB ligand (RANKL) production and its combination with TNF-stimulated gene 6(TSG-6) could improve activity of osteoclasts and promote bone resorption;for mesenchymal stem cells (MSCs), PTX3 could promote osteogenic differentiation of MSCs through PI3K/Akt signaling pathway. In recent years, the role of PTX3 as a new bone metabolism regulator in OP and fracture healing has been gradually concerned by scholars. In OP patients, PTX3 regulates bone mass mainly by promoting bone regeneration. In the process of fracture healing, PTX3 promotes fracture healing by coordinating bone regeneration and bone resorption to maintain bone homeostasis. In view of the above biological characteristics, PTX3 is expected to become a new target for the diagnosis and treatment of OP and other age-related bone diseases and fracture healing.
MeSH term(s)	Animals ; Male ; Mice ; Bone Resorption/metabolism ; Cell Differentiation ; Fracture Healing/genetics ; Osteoblasts ; Osteoclasts ; Osteogenesis ; Osteoporosis/genetics ; Phosphatidylinositol 3-Kinases/metabolism ; Phosphatidylinositol 3-Kinases/pharmacology
Chemical Substances	Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; PTX3 protein (148591-49-5)
Language	Chinese
Publishing date	2023-04-17
Publishing country	China
Document type	English Abstract ; Journal Article
ISSN	1003-0034
ISSN	1003-0034
DOI	10.12200/j.issn.1003-0034.2023.04.018
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Article: Algorithm for identifying wind power ramp events via novel improved dynamic swinging door

Cui, Yang / He, Yingjie / Xiong, Xiong / Chen, Zhenghong / Li, Fen / Xu, Taotao / Zhang, Fanghong

Renewable energy. 2021 June, v. 171

2021

Abstract: With the rapid increase in the penetration of wind power in recent years, wind power ramp events (WPREs) have become the main factors affecting the safety and stability of electric power systems. Accurate detection of ramp events could help power systems ...

Abstract	With the rapid increase in the penetration of wind power in recent years, wind power ramp events (WPREs) have become the main factors affecting the safety and stability of electric power systems. Accurate detection of ramp events could help power systems better manage the extreme events and reduce economic losses. The previous ramp detection methods are either too complex to implement that influence the computing efficiency, or based on the value of points which cannot completely reflect the trend of data segments and lead to a decrease of accuracy. Based on the above problems and the on-site requirements, this paper proposes a novel improved dynamic swinging door algorithm (ImDSDA) to optimise the state-of-the-art in WPREs detection. Firstly, the swinging door algorithm (SDA) is used to extract ramp segments. Secondly, the dynamic programming method is used for ramp trend identification and segment combination. Finally, raw data obtained from three real-world wind farms in Hubei, China were applied to validate the performance of the proposed ImDSDA. The detection results show that the ImDSDA is more accurate and efficient than the traditional detection methods and could be a feasible option for WPRE detection in power systems.
Keywords	algorithms ; electric power ; wind ; wind power ; China
Language	English
Dates of publication	2021-06
Size	p. 542-556.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	2001449-1
ISSN	1879-0682 ; 0960-1481
ISSN (online)	1879-0682
ISSN	0960-1481
DOI	10.1016/j.renene.2021.02.123
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Article: Cellulose nanofibers coated with silver nanoparticles as a flexible nanocomposite for measurement of flusilazole residues in Oolong tea by surface-enhanced Raman spectroscopy

Chen, Xi / Lin, Hetong / Xu, Taotao / Lai, Keqiang / Han, Xue / Lin, Mengshi

Food chemistry. 2020 June 15, v. 315

2020

Abstract: Cellulose nanofibers (CNF) coated with inorganic nanoparticles are novel hybrid nanocomposites that have great potential in various areas including agriculture and food science. The objectives of this study were to synthesize nanocomposites consisted of ... ...

Abstract	Cellulose nanofibers (CNF) coated with inorganic nanoparticles are novel hybrid nanocomposites that have great potential in various areas including agriculture and food science. The objectives of this study were to synthesize nanocomposites consisted of CNF coated with silver nanoparticles (AgNPs), which can be used as a surface-enhanced Raman spectroscopy (SERS) platform for measuring pesticides in Oolong tea. CNF were coated with AgNPs to form uniform CNF-AgNP nanocomposites that were investigated by transmission electron microscopy. Three-dimensional and porous CNF structures were loaded with AgNPs with an average size of 41 nm. CNF-AgNP substrates were applied in characterization and measurement of flusilazole in Oolong tea samples by SERS. A detection limit of 0.5 mg/kg for flusilazole was obtained based on partial least squares (PLS) regression analysis. These results indicate that CNF-AgNP nanocomposites combined with SERS is an accurate, sensitive, and efficient technique for identification and quantification of pesticide residues in Oolong tea.
Keywords	Raman spectroscopy ; cellulose nanofibers ; detection limit ; flusilazole ; food science ; fungicide residues ; least squares ; nanocomposites ; nanoparticles ; nanosilver ; oolong tea ; transmission electron microscopy
Language	English
Dates of publication	2020-0615
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2020.126276
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Article: Essence of "Shen (Kidney) Controlling Bones": Conceptual Analysis Based on Hypothalamic-Pituitary-Adrenal-Osteo-Related Cells Axis.

Xu, Tao-Tao / Jin, Hong-Ting / Tong, Pei-Jian

Chinese journal of integrative medicine

2018 Volume 24, Issue 11, Page(s) 806–808

Abstract: As a traditional concept of Chinese medicine (CM), the theory of "Shen (Kidney) controlling bones" has been gradually proven. And in modern allopathic medicine, the multiple mechanisms of bone growth, development and regeneration align with the theory. ... ...

Abstract	As a traditional concept of Chinese medicine (CM), the theory of "Shen (Kidney) controlling bones" has been gradually proven. And in modern allopathic medicine, the multiple mechanisms of bone growth, development and regeneration align with the theory. Shen deficiency as a pathological condition has a negative effect on the skeleton of body, specifically the disorder of bone homeostasis. Present studies indicate that Shen deficiency shares a common disorder characterized by dysfunction of hypothalamic-pituitary-adrenal (HPA) axis. HPA axis may be an important regulator of bone diseases with abnormal homeostasis. Therefore, we posit the existence of hypothalamic-pituitary-adrenal-osteo-related cells axis: cells that comprise bone tissue (osteo-related cells) are targets under the regulation of HPA axis in disorder of bone homeostasis. Chinese herbs for nourishing Shen have potential in the development of treatments for disorder of bone homeostasis.
MeSH term(s)	Bone Development ; Bone Diseases/etiology ; Homeostasis ; Humans ; Hypothalamo-Hypophyseal System/physiology ; Kidney/physiology ; Medicine, Chinese Traditional ; Pituitary-Adrenal System/physiology
Language	English
Publishing date	2018-04-12
Publishing country	China
Document type	Journal Article
ZDB-ID	2171254-2
ISSN	1993-0402 ; 1672-0415
ISSN (online)	1993-0402
ISSN	1672-0415
DOI	10.1007/s11655-018-2930-x
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Zs.A 5823: Show issues

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Article: Enhanced osteogenesis of hydroxyapatite scaffolds by coating with BMP-2-loaded short polylactide nanofiber: a new drug loading method for porous scaffolds.

Xu, Taotao / Sheng, Luyao / He, Lei / Weng, Jie / Duan, Ke

Regenerative biomaterials

2019 Volume 7, Issue 1, Page(s) 91–98

Abstract: Porous hydroxyapatite (HA) is widely used in porous forms to assist bone defect healing. However, further improvements in biological functions are desired for meeting complex clinical situations such as impaired bone regeneration in poor bone stock. The ... ...

Abstract	Porous hydroxyapatite (HA) is widely used in porous forms to assist bone defect healing. However, further improvements in biological functions are desired for meeting complex clinical situations such as impaired bone regeneration in poor bone stock. The extracellular matrix (ECM) of human tissues is characterized by nanofibrous structures and a variety of signal molecules. Emulating these characteristics are expected to create a favorable microenvironment for cells and simultaneously allow release of osteogenic molecules. In this study, short polylactide fibers containing BMP-2 were prepared by electrospinning and coated on porous HA scaffolds. The coating did not affect porosity or pore interconnectivity of the scaffold but improved its compressive strength markedly. This fiber coating produced burst BMP-2 release in 1 day followed by a linear release for 24 days. The coating had a significantly lower rat calvarial osteoblasts (RCOBs) adhesion (vs. uncoated scaffold) but allowed normal proliferation subsequently. Bone marrow stem cells (MSCs) on the coated scaffolds expressed a significantly increased alkaline phosphatase activity relative to the uncoated ones. After implantation in canine dorsal muscles, the coated scaffolds formed significantly more new bone at Weeks 4 and 12, and more blood vessels at Week 12. This method offers a new option for drug delivery systems.
Language	English
Publishing date	2019-11-07
Publishing country	England
Document type	Journal Article
ZDB-ID	2799042-4
ISSN	2056-3426 ; 2056-3418
ISSN (online)	2056-3426
ISSN	2056-3418
DOI	10.1093/rb/rbz040
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