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Article ; Online: The critical role of toll-like receptor 4 in bone remodeling of osteoporosis: from inflammation recognition to immunity.

Zhu, Xianping / Du, Li / Zhang, Lai / Ding, Lingzhi / Xu, Weifang / Lin, Xuezheng

2024 Volume 15, Page(s) 1333086

Abstract: Osteoporosis is a common chronic metabolic bone disorder. Recently, increasing numbers of studies have demonstrated that Toll-like receptor 4 (TLR4, a receptor located on the surface of osteoclasts and osteoblasts) plays a pivotal role in the development ...

Abstract	Osteoporosis is a common chronic metabolic bone disorder. Recently, increasing numbers of studies have demonstrated that Toll-like receptor 4 (TLR4, a receptor located on the surface of osteoclasts and osteoblasts) plays a pivotal role in the development of osteoporosis. Herein, we performed a comprehensive review to summarize the findings from the relevant studies within this topic. Clinical data showed that TLR4 polymorphisms and aberrant TLR4 expression have been associated with the clinical significance of osteoporosis. Mechanistically, dysregulation of osteoblasts and osteoclasts induced by abnormal expression of TLR4 is the main molecular mechanism underlying the pathological processes of osteoporosis, which may be associated with the interactions between TLR4 and NF-κB pathway, proinflammatory effects, ncRNAs, and RUNX2.
MeSH term(s)	Humans ; Toll-Like Receptor 4/metabolism ; Osteoclasts/metabolism ; Bone Remodeling ; Osteoporosis/metabolism ; Inflammation/metabolism
Chemical Substances	Toll-Like Receptor 4
Language	English
Publishing date	2024-03-05
Publishing country	Switzerland
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2024.1333086
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Article ; Online: Identification of immune-related gene signatures for chronic obstructive pulmonary disease with metabolic syndrome: evidence from integrated bulk and single-cell RNA sequencing data.

Wu, Yueren / Ma, Mengyu / Choi, Wenglam / Xu, Weifang / Dong, Jingcheng

International immunology

2023 Volume 36, Issue 1, Page(s) 17–32

Abstract: Chronic obstructive pulmonary disease (COPD) is closely related to innate and adaptive inflammatory immune responses. It is increasingly becoming evident that metabolic syndrome (MetS) affects a significant portion of COPD patients. Through this ... ...

Abstract	Chronic obstructive pulmonary disease (COPD) is closely related to innate and adaptive inflammatory immune responses. It is increasingly becoming evident that metabolic syndrome (MetS) affects a significant portion of COPD patients. Through this investigation, we identify shared immune-related candidate biological markers. The Weighted Gene Co-Expression Network Analysis (WGCNA) was utilized to reveal the co-expression modules linked to COPD and MetS. The commonly expressed genes in the COPD and MetS were utilized to conduct an enrichment analysis. We adopted machine-learning to screen and validate hub genes. We also assessed the relationship between hub genes and immune cell infiltration in COPD and MetS, respectively. Moreover, associations across hub genes and metabolic pathways were also explored. Finally, we chose a single-cell RNA sequencing (scRNA-seq) dataset to investigate the hub genes and shared mechanisms at the level of the cells. We also applied cell trajectory analysis and cell-cell communication analysis to focus on the vital immune cell we were interested in. As a result, we selected and validated 13 shared hub genes for COPD and MetS. The enrichment analysis and immune infiltration analysis illustrated strong associations between hub genes and immunology. Additionally, we applied metabolic pathway enrichment analysis, indicating the significant role of reactive oxygen species (ROS) in COPD with MetS. Through scRNA-seq analysis, we found that ROS might accumulate the most in the alveolar macrophages. In conclusion, the 13 hub genes related to the immune response and metabolism may serve as diagnostic biomarkers and treatment targets of COPD with MetS.
MeSH term(s)	Humans ; Metabolic Syndrome/genetics ; Reactive Oxygen Species ; Cell Communication ; Pulmonary Disease, Chronic Obstructive/genetics ; Sequence Analysis, RNA
Chemical Substances	Reactive Oxygen Species
Language	English
Publishing date	2023-10-16
Publishing country	England
Document type	Journal Article
ZDB-ID	1013745-2
ISSN	1460-2377 ; 0953-8178
ISSN (online)	1460-2377
ISSN	0953-8178
DOI	10.1093/intimm/dxad043
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Article ; Online: Distinct spatial and temporal roles for Th1, Th2, and Th17 cells in asthma.

Luo, Weihang / Hu, Jindong / Xu, Weifang / Dong, Jingcheng

Frontiers in immunology

2022 Volume 13, Page(s) 974066

Abstract: Immune response in the asthmatic respiratory tract is mainly driven by ... ...

Abstract	Immune response in the asthmatic respiratory tract is mainly driven by CD4
MeSH term(s)	Asthma/immunology ; Cytokines ; Humans ; Th1 Cells/immunology ; Th17 Cells/immunology ; Th2 Cells/immunology
Chemical Substances	Cytokines
Language	English
Publishing date	2022-08-12
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.974066
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Article ; Online: First Report of Root Rot Caused by Pleiocarpon algeriense on Peony (Paeonia suffruticosa) in China

Ni, Wenjie / Zhao, Hongsu / Xu, Weifang / Xu, Fengqing / Huang, Shunwang / Wang, Renzhong / Wu, Deling

Plant Disease. 2023 Sept. 01, v. 107, no. 9 p.2858-

2023

Abstract: Peony (Paeonia suffruticosa Andr.) is a perennial plant of the family Ranunculaceae. Its root bark (Danpi in Chinese) is a traditional Chinese medicine, which has the effects of clearing heat and cooling blood, promoting blood circulation to resolve ... ...

Abstract	Peony (Paeonia suffruticosa Andr.) is a perennial plant of the family Ranunculaceae. Its root bark (Danpi in Chinese) is a traditional Chinese medicine, which has the effects of clearing heat and cooling blood, promoting blood circulation to resolve blood stasis. Peony is mainly planted in the provinces of Anhui, Gansu, Henan, and Shandong. Peony is also called Fengdan in Fenghuang Mountain of Tongling, Anhui Province. In November 2021, a root rot-like disease was observed on the root of peony in several fields located in Tongling County, Anhui Province, China (30°48′11″N, 118°0′51″E). Approximately 20 to 40% of the peony plants were affected in the fields. The roots of the diseased plants were rotten and blackened, bark of the roots detached, and leaves withered, causing the death of whole plants. To isolate the pathogen, the symptomatic roots were sampled, and small pieces (5 × 5 mm) of diseased tissues were surface sterilized with 0.5% NaClO solution and 75% ethanol for 5 min, rinsed with sterile distilled water three times, and finally incubated on potato dextrose agar at 28°C in the dark for 7 days. A total of 16 isolates were obtained from the infected tissues. Among the isolates, six isolates were morphologically similar to B4. Colonies were passaged multiple times on fresh PDA medium, and a pure isolate (B4) exhibiting cinnamon-to-honey coloration on PDA with pale yellow aerial hyphae was then selected. Microscopic observations revealed that microconidia were straight to curved, ellipsoid or subcylindrical, and ranged from 7.14 to 14.29 × 2.85 to 5.00 μm (n = 20). The morphological characteristics were similar to the description of Pleiocarpon algeriense by Aigoun-Mouhous et al. (2019). To further identify the taxonomic status of the B4 strain, three genes of the internal transcribed spacer (ITS) region of rDNA, beta-tubulin (TUB2), and RNA polymerase II second subunit (RPB2) were amplified and sequenced using the primers ITS1/ITS4 (White et al. 1990), T1/Bt-2b (O’Donnell and Cigelnik 1997), and 5F2/7cR (O’Donnell et al. 2007), respectively. Sequences for the isolate B4 were deposited in GenBank (OP810684, ITS; OP882301, TUB2; OP863337, RPB2). BLAST analysis showed the ITS, TUB2, RPB2 sequences of B4 had 99.80% (505/506), 99.51% (609/612), and 100.00% (854/854) homology with those of P. algeriense Di3A-AP52 (MT613337, ITS; MT597145, TUB2; MT635004, RPB2). A phylogenetic tree was built using MEGA11 based on sequences of the three genes, showing that the B4 strain closely clustered with the reference strain of P. algeriense, which has not been reported in peony in China. The pathogenicity test of the isolates was performed by inoculating 50 ml of conidial suspension (1 × 10⁸ conidia/ml) on the roots of 10 healthy peonies, and 10 peonies inoculated with 50 ml of sterile water were used as a control group. After 1 month, typical symptoms of root rot appeared on the inoculated plants, whereas the control plants were asymptomatic. The fungus (P. algeriense) was reisolated from the diseased roots and identified by sequencing the ITS gene, fulfilling Koch’s postulates. P. algeriense has been reported to cause stem and crown rot in avocado (Aiello et al. 2020). To the best of our knowledge, this is the first report of P. algeriense causing root rot in peony. Control methods of P. algeriense on peony fields will be studied in depth in the future.
Keywords	DNA-directed RNA polymerase ; Oriental traditional medicine ; Paeonia suffruticosa ; Ranunculaceae ; avocados ; bark ; blood ; blood circulation ; blood stasis ; color ; conidia ; crown rot ; culture media ; death ; ethanol ; fungi ; genes ; heat ; hyphae ; internal transcribed spacers ; pathogenicity ; pathogens ; perennials ; phylogeny ; root rot ; tubulin ; China ; fungus ; peony ; Pleiocarpon algeriense
Language	English
Dates of publication	2023-0901
Publishing place	The American Phytopathological Society
Document type	Article ; Online
ZDB-ID	754182-x
ISSN	0191-2917
ISSN	0191-2917
DOI	10.1094/PDIS-03-23-0425-PDN
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: The risk factors of postoperative delirium in patients with hip fracture: implication for clinical management.

Xu, Weifang / Ma, Haiping / Li, Wang / Zhang, Chen

BMC musculoskeletal disorders

2021 Volume 22, Issue 1, Page(s) 254

Abstract: Background: Delirium is a common complication of hip surgery patients. It is necessary to investigate the epidemiological characteristics and related risk factors of delirium after hip fracture surgery, to provide evidence supports for the prevention ... ...

Abstract	Background: Delirium is a common complication of hip surgery patients. It is necessary to investigate the epidemiological characteristics and related risk factors of delirium after hip fracture surgery, to provide evidence supports for the prevention and management of delirium. Methods: Hip fracture patients admitted to our hospital for surgical treatment from March 2018 to March 2020 were identified as participants. The characteristics and laboratory examinations in patients with and without postoperative delirium were compared and analyzed. Logistic regression analyses were conducted to ascertain the independent risk factors, and the area under the curve (AUC) were calculated to analyze the predictive value. Results: A total of 568 postoperative patients with hip fracture were included, the incidence of delirium in postoperative patients with hip fracture was 14.44 %. The preoperative albumin (OR 4.382, 2.501 ~ 5.538), history of delirium (OR 2.197, 1.094 ~ 3.253), TSH (OR1.245, 1.077 ~ 1.638), the resting score on the first postoperative day (OR1.235, 0.944 ~ 1.506) and age(OR1.185, 0.065 ~ 1.814) were the independent risk factors for the postoperative delirium in patients with hip fracture(all p < 0.05). The AUC of albumin, history of delirium, TSH, the resting score on the first postoperative day and age were 0.794, 0.754, 0.746, 0.721 and 0.689 respectively. Conclusions: The incidence of delirium in postoperative patients with hip fracture is rather high, especially for patients with old age and history of delirium. Monitoring albumin, TSH and resting score may be beneficial to the management of postoperative delirium.
MeSH term(s)	Delirium/diagnosis ; Delirium/epidemiology ; Delirium/etiology ; Hip Fractures/epidemiology ; Hip Fractures/surgery ; Humans ; Incidence ; Postoperative Complications/diagnosis ; Postoperative Complications/epidemiology ; Postoperative Complications/etiology ; Risk Factors
Language	English
Publishing date	2021-03-07
Publishing country	England
Document type	Journal Article
ISSN	1471-2474
ISSN (online)	1471-2474
DOI	10.1186/s12891-021-04091-1
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Article: Prevalence of osteoporosis in patients with nephrolithiasis and

Jia, Shunjie / Liao, Jian / Wang, Yucheng / Zheng, Wenbiao / Jin, Jinghua / Xu, Weifang / Zheng, Qi

Frontiers in endocrinology

2023 Volume 14, Page(s) 1180183

Abstract: Purpose: Nephrolithiasis is thought to be a risk factor for osteoporosis, but data assessing if osteoporosis predisposes to the risk of nephrolithiasis are lacking. The present study aims to investigate whether patients with nephrolithiasis have a ... ...

Abstract	Purpose: Nephrolithiasis is thought to be a risk factor for osteoporosis, but data assessing if osteoporosis predisposes to the risk of nephrolithiasis are lacking. The present study aims to investigate whether patients with nephrolithiasis have a prominently higher prevalence of osteoporosis than the controls and Methods: Four databases were used to detect the eligible studies. We calculated the relative risk (RR) with a 95% confidence interval (CI) to assess the combined effect. The methodologies for conducting this study followed the PRISMA guidelines and were registered in the PROSPERO (ID: CRD42023395875). Results: Nine case-control or cohort studies with a total of 454,464 participants were finally included. Combined results indicated that there was a significantly higher prevalence of osteoporosis in patients with nephrolithiasis as compared to the general population without nephrolithiasis (overall RR from six studies= 1.204, 95%CI: 1.133 to 1.28, Conclusions: The present study highlighted a significantly high prevalence of osteoporosis in patients with nephrolithiasis and Systematic review registration: https://www.crd.york.ac.uk/PROSPERO/#searchadvanced, identifier CRD42023395875.
MeSH term(s)	Humans ; Aged ; Prevalence ; Osteoporosis/epidemiology ; Osteoporosis/diagnosis ; Kidney Calculi ; Cohort Studies ; Risk Factors
Language	English
Publishing date	2023-07-04
Publishing country	Switzerland
Document type	Systematic Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2592084-4
ISSN	1664-2392
ISSN	1664-2392
DOI	10.3389/fendo.2023.1180183
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Article: First Report of Root Rot Caused by Pleiocarpon algeriense on peony (Paeonia suffruticosa) in China.

Ni, Wenjie / Zhao, Hongsu / Xu, Weifang / Xu, Fengqing / Huang, Shunwang / Wang, Renzhong / Wu, Deling

Plant disease

2023

Abstract: Peony (Paeonia suffruticosa Andr.) is a perennial plant of Ranunculaceae. Its root bark (Danpi in Chinese) is a traditional Chinese medicine, which has the effects of clearing heat and cooling blood, promoting blood circulation to resolve blood stasis. ... ...

Abstract	Peony (Paeonia suffruticosa Andr.) is a perennial plant of Ranunculaceae. Its root bark (Danpi in Chinese) is a traditional Chinese medicine, which has the effects of clearing heat and cooling blood, promoting blood circulation to resolve blood stasis. Peony is mainly planted in the provinces of Anhui, Gansu, Henan and Shandong. Peony is also called Fengdan in the Fenghuang Mountain of Tongling, Anhui Province. In November 2021, a root rot-like disease was observed on the root of peony in several fields located in Tongling county, Anhui Province, China (118°0'51" N, 30°48'11" E). Approximately 20-40% of the peony plants were affected in the fields. The roots of the diseased plants were rotten and blackened, the bark of the roots was detached, and the leaves were withered, causing the whole plants to die. To isolate the pathogen, the symptomatic roots were sampled, and small pieces (5 × 5 mm) of diseased tissues were surface sterilized with 0.5% NaClO solution and 75% ethanol for 5 min, rinsed with sterile distilled water three times, and finally incubated on potato dextrose agar (PDA) at 28°C in the dark for 7 days. A total of 16 isolates were obtained from the infected tissues. Among isolates, six isolates were morphologically similar to B4. Colonies were passaged multiple times on fresh PDA medium, and pure isolate B4 exhibiting cinnamon-to-honey coloration on PDA with pale yellow aerial hyphae, was then selected. Microscopic observations revealed that microconidia were straight to curved, ellipsoid or subcylindrical, and ranged from 7.14 to 14.29 × 2.85 to 5.00 μm (n = 20). The morphological characteristics were similar to the description of Pleiocarpon algeriense by Aigoun-Mouhous et al. (2019). To further identify the taxonomic status of B4 strain, three genes of the internal transcribed spacer (ITS) region of rDNA, beta-tubulin (TUB2), and the RNA polymerase II second subunit (RPB2) were respectively amplified and sequenced using primers ITS1/ITS4 (White et al. 1990), T1/Bt-2b (O'Donnell and Cigelnik 1997), and 5F2/7cR (O'Donnell et al. 2007). Sequences for the isolate B4 were deposited in GenBenk (OP810684, ITS; OP882301, TUB2; OP863337, RPB2). BLAST analysis showed the ITS, TUB2, RPB2 sequences of B4 were 99.80% (505/506), 99.51% (609/612) and 100.00% (854/854) homology with those of P. algeriense Di3A-AP52 (MT613337, ITS; MT597145, TUB2; MT635004, RPB2). A phylogenetic tree was built using MEGA11 based on sequences of three genes showing that B4 strain was closely clustered with reference strain of P. algeriense, which has not been reported in peony in China. The pathogenicity test of the isolates was performed by inoculating 50 mL of conidial suspension (1 × 108 conidia/mL) on the roots of ten healthy peonies, ten peonies inoculated with 50 mL of sterile water were used as a control group. After one-month, typical symptoms of root rot appeared on the inoculated plants and the control plants were asymptomatic. The fungus (P. algeriense) was reisolated from the diseased roots and identified by sequencing of ITS gene, conforming to Koch's postulates. Pleiocarpon algeriense has been reported to cause stem and crown rot in avocado (Aiello et al. 2020). To the best of our knowledge, this is the first report of P. algeriense causing root rot in peony. Control methods of P. algeriense on peony fields will be studied in-depth in the future.
Language	English
Publishing date	2023-04-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	754182-x
ISSN	0191-2917
ISSN	0191-2917
DOI	10.1094/PDIS-03-23-0425-PDN
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Article ; Online: Diagnostic efficacy of serum IL-17 and NF-κB expression levels in early detection of lung consolidation in children with Mycoplasma pneumoniae pneumonia

Li Linnan / Wang Yongfang / Cheng Yanbo / Xu Yufang / Xu Weifang / Li Xia / Zhang Chengyun / Ran Shaoyun

Xin yixue, Vol 54, Iss 5, Pp 350-

2023 Volume 353

Abstract: Objective To investigate the expression levels and significance of serum interleukin-17 （IL-17） and nuclear factor-κB （NF-κB） in Mycoplasma pneumoniae pneumonia （MPP） children in acute stage with lung consolidation. Methods Sixty-four children diagnosed ... ...

Abstract	Objective To investigate the expression levels and significance of serum interleukin-17 （IL-17） and nuclear factor-κB （NF-κB） in Mycoplasma pneumoniae pneumonia （MPP） children in acute stage with lung consolidation. Methods Sixty-four children diagnosed with MPP in acute stage were selected. According to the imaging features of chest CT scan or chest X-ray upon admission， all patients were divided into the lung consolidation group （n = 32） and non-lung consolidation group （n = 32）. The serum levels of IL-17 and NF-κB in MPP children were detected and compared between two groups. The relationship between IL-17 and NF-κB was analyzed. The receiver operating characteristic （ROC） curve was drawn. The diagnostic efficacy of serum IL-17 and NF-κB levels for lung consolidation was assessed in MPP children in acute stage. Results The serum levels of IL-17 and NF-κB in the lung consolidation group were higher than those in the non-lung consolidation group （both P < 0.05）. Spearman correlation analysis showed that IL-17 level was positively correlated with NF-κB level in MPP children （rs = 0.818， P < 0.05）. When the serum level of IL-17 was 10.45 pg/mL， the area under the ROC curve （AUC） was 0.806， and the sensitivity and specificity for the diagnosis of lung consolidation in MPP children were 75.0% and 78.1%. When the serum level of NF-κB was 534.13 pg/mL， the AUC was 0.951， and the sensitivity and specificity for the diagnosis of lung consolidation in children with MPP were 90.6% and 84.4%. Conclusions The expression levels of IL-17 and NF-κB are up-regulated in MPP children with lung consolidation in acute stage. Early detection of serum levels of IL-17 and NF-κB has certain diagnostic efficacy for lung consolidation in MPP.
Keywords	\|mycoplasma pnuemoniae pneumonia\|lung consolidation\|interleukin-17\|nuclear factor-κb\|diagnosis ; Medicine ; R
Subject code	610
Language	Chinese
Publishing date	2023-05-01T00:00:00Z
Publisher	Editorial Office of Journal of New Medicine
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Network pharmacology-based prediction and validation of the active ingredients and potential mechanisms of the Huangxiong formula for treating ischemic stroke

Zhao, Saihong / Zhang, Pingping / Yan, Yonghuan / Xu, Weifang / Li, Jiacheng / Wang, Lei / Wang, Ning / Huang, Yingying

Journal of Ethnopharmacology. 2023 Apr. 18, p.116507-

2023 , Page(s) 116507–

Abstract: Huangxiong Formula (HXF) is composed of four herbs: Rheum palmatum L., Ligusticum striatum DC., Curcuma aromatica Salisb., and Acorus gramineus Aiton. HXF is clinically used for the treatment of ischemic stroke (IS). However, its molecular mechanism ... ...

Abstract	Huangxiong Formula (HXF) is composed of four herbs: Rheum palmatum L., Ligusticum striatum DC., Curcuma aromatica Salisb., and Acorus gramineus Aiton. HXF is clinically used for the treatment of ischemic stroke (IS). However, its molecular mechanism remains unclear. A network pharmacology-based strategy combined with experimental study in vivo and in vitro to were used to investigate the bioactive components, potential targets, and molecular mechanisms of HXF in the treatment of IS. The components of HXF were detected by ultra-performance liquid chromatography (UPLC). The potential active ingredients of HXF were acquired from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and literature, and corresponding targets were discerned through the Swiss TargetPrediction database. IS-related targets were obtained from Genecards, Online Mendelian Inheritance in Man (OMIM), Therapeutic Target Database (TTD), and DisGeNET. The intersection of ingredient and disease targets was screened, and a herbal-compound-target network was constructed. A protein-protein interaction (PPI) network was created, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed. Based on these analyses, we established a compound-target-pathway (C-T-P) network. A cerebral ischemia-reperfusion (I/R) animal model was established, and the cerebral protective effect of HXF was assessed. The accuracy of the predicted targets was verified by real-time quantitative polymerase chain reaction (RT-qPCR). Hippocampal neuronal injury cell model induced by oxygen-glucose deprivation and reperfusion (OGD/R) was used to evaluate the protective effect of α-Asarone. Furthermore, molecular docking, drug affinity responsive target stability (DARTS) assay, and cellular thermal shift assay (CETSA) were performed to verify whether α-Asarone can bind to PI3K. A total of 44 active ingredients and 795 gene targets were identified through network pharmacology. Network analysis showed that naringenin, eupatin, kaempferol, and α-Asarone were possible drug candidates. SRC, AKT1, TP53, MAPK3, STAT3, HRAS, CTNNB1, EGFR, VEGFA, PIK3R1 could serve as potential drug targets. KEGG analysis implied that the PI3K/AKT signaling pathway might play an important role in treating IS by HXF. Moreover, HXF significantly reduced neurological impairment, cerebral infarct volume, brain index, and brain histopathological damage in I/R rats. The mRNA expression of the top 10 potential targets was verified in the brain tissue. The C-T-P network and UPLC analysis suggested that α-Asarone might be an important component of HXF and can inhibit oxidative stress and apoptosis in HT22 cells by activating the PI3K/AKT signaling pathway. Molecular docking, DARTS, and CETSA assay analysis confirmed that there were direct interactions between α-Asarone and PI3K. HXF had a therapeutic effect in IS with multi-component, multi-target, and multi-approach features. α-Asarone, identified as one of the major active components of HXF, could alleviate oxidative stress and apoptosis by targeting PI3K/AKT pathway.
Keywords	Acorus gramineus ; Curcuma aromatica ; Mendelian inheritance ; Oreocome striata ; Oriental traditional medicine ; Rheum palmatum ; animal models ; apoptosis ; brain ; drugs ; gene expression ; gene ontology ; genes ; histopathology ; infarction ; ingredients ; kaempferol ; naringenin ; neurons ; oxidative stress ; pharmacology ; phosphatidylinositol 3-kinase ; prediction ; protective effect ; protein-protein interactions ; quantitative polymerase chain reaction ; stroke ; therapeutics ; ultra-performance liquid chromatography ; Huangxiong formula ; Ischemic stroke ; Network pharmacology ; PI3K/AKT signal Pathway ; α-Asarone
Language	English
Dates of publication	2023-0418
Publishing place	Elsevier B.V.
Document type	Article ; Online
Note	Pre-press version
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.116507
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Risk of osteoporosis and fracture after hysterectomies without oophorectomies: a systematic review and pooled analysis.

Xu, Weifang / Wu, Weizhou / Yang, Suqing / Chen, Tingting / Teng, Xiao / Gao, Danping / Zhao, Shankun

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA

2022 Volume 33, Issue 8, Page(s) 1677–1686

Abstract: The present study provides evidence that women who underwent hysterectomy without oophorectomies are at a higher risk of osteoporosis and bone fractures than the general population. Early interventions for these susceptible women may help to delay or ... ...

Abstract	The present study provides evidence that women who underwent hysterectomy without oophorectomies are at a higher risk of osteoporosis and bone fractures than the general population. Early interventions for these susceptible women may help to delay or reduce the risk of osteoporosis and bone fractures. Introduction: Mounting studies have shown that patients with hysterectomy are at high risk of developing osteoporosis or bone fractures, but the evidence from all the relevant studies has not been previously synthesized. The present study aims to investigate whether women with hysterectomy without oophorectomies have a prominently higher prevalence of osteoporosis or fractures than healthy subjects. Methods: Four electronic databases were systematically searched to identify the eligible studies. The combined effect was assessed by calculating the relative risk (RR) with a 95% confidence interval (CI). More methodologies for this study were available in the PROSPERO (ID: CRD42021227255). Results: Finally, three observational studies offering osteoporosis cases and two retrospective studies reporting fracture cases were included. One eligible study has provided independent data from three groups of fractures. Synthetic results revealed that hysterectomy without oophorectomies was significantly associated with an increased risk of osteoporosis as compared to the general population (combined RR from three studies = 1.47, 95%CI 1.253 to 1.725, P < 0.001; heterogeneity, I Conclusions: This is the first study to quantify the association between hysterectomy without oophorectomies and osteoporosis/fracture risk through a meta-analysis and has subsequently confirmed its positive relationship. Additional large-sample rigorously prospective cohorts are still warranted to validate the present evidence.
MeSH term(s)	Female ; Humans ; Hysterectomy/adverse effects ; Osteoporosis/complications ; Osteoporosis/epidemiology ; Osteoporotic Fractures/epidemiology ; Osteoporotic Fractures/etiology ; Osteoporotic Fractures/surgery ; Ovariectomy ; Prospective Studies ; Retrospective Studies
Language	English
Publishing date	2022-03-29
Publishing country	England
Document type	Journal Article ; Meta-Analysis ; Review ; Systematic Review
ZDB-ID	1064892-6
ISSN	1433-2965 ; 0937-941X
ISSN (online)	1433-2965
ISSN	0937-941X
DOI	10.1007/s00198-022-06383-1
Database	MEDical Literature Analysis and Retrieval System OnLINE

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