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  1. Article ; Online: Ginkgolide B can alleviate spinal cord glymphatic system dysfunction and provide neuroprotection in painful diabetic neuropathy rats by inhibiting matrix metalloproteinase-9.

    Li, Jiang / Jia, Shuaiying / Song, Yuehan / Xu, Wenmei / Lin, Jingyan

    Neuropharmacology

    2024  Volume 250, Page(s) 109907

    Abstract: The glymphatic system plays a crucial role in maintaining optimal central nervous system (CNS) function by facilitating the removal of metabolic wastes. Aquaporin-4 (AQP4) protein, predominantly located on astrocyte end-feet, is a key pathway for ... ...

    Abstract The glymphatic system plays a crucial role in maintaining optimal central nervous system (CNS) function by facilitating the removal of metabolic wastes. Aquaporin-4 (AQP4) protein, predominantly located on astrocyte end-feet, is a key pathway for metabolic waste excretion. β-Dystroglycan (β-DG) can anchor AQP4 protein to the end-feet membrane of astrocytes and can be cleaved by matrix metalloproteinase (MMP)-9 protein. Studies have demonstrated that hyperglycemia upregulates MMP-9 expression in the nervous system, leading to neuropathic pain. Ginkgolide B (GB) exerts an inhibitory effect on the MMP-9 protein. In this study, we investigated whether inhibition of MMP-9-mediated β-DG cleavage by GB is involved in the regulation of AQP4 polarity within the glymphatic system in painful diabetic neuropathy (PDN) and exerts neuroprotective effects. The PDN model was established by injecting streptozotocin (STZ). Functional changes in the glymphatic system were observed using magnetic resonance imaging (MRI). The paw withdrawal threshold (PWT) was measured to assess mechanical allodynia. The protein expressions of MMP-9, β-DG, and AQP4 were detected by Western blotting and immunofluorescence. Our findings revealed significant decreases in the efficiency of contrast agent clearance within the spinal glymphatic system of the rats, accompanied by decreased PWT, increased MMP-9 protein expression, decreased β-DG protein expression, and loss of AQP4 polarity. Notably, GB treatment demonstrated the capacity to ameliorate spinal cord glymphatic function by modulating AQP4 polarity through MMP-9 inhibition, offering a promising therapeutic avenue for PDN.
    MeSH term(s) Rats ; Animals ; Glymphatic System/metabolism ; Matrix Metalloproteinase 9 ; Neuroprotection ; Diabetic Neuropathies/drug therapy ; Diabetic Neuropathies/metabolism ; Spinal Cord/metabolism ; Aquaporin 4/metabolism ; Diabetes Mellitus ; Lactones ; Ginkgolides
    Chemical Substances Matrix Metalloproteinase 9 (EC 3.4.24.35) ; ginkgolide B (DF149B9460) ; Aquaporin 4 ; Lactones ; Ginkgolides
    Language English
    Publishing date 2024-03-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 218272-5
    ISSN 1873-7064 ; 0028-3908
    ISSN (online) 1873-7064
    ISSN 0028-3908
    DOI 10.1016/j.neuropharm.2024.109907
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  2. Article ; Online: Effect of deep neuromuscular block on the quality of early recovery after sleeve gastrectomy in obese patients: a randomized controlled trial.

    Yang, Wan-Li / Wen, Ya-Ling / Xu, Wen-Mei / Xu, Chi-Liang / Yin, Wen-Qin / Lin, Jing-Yan

    BMC anesthesiology

    2024  Volume 24, Issue 1, Page(s) 101

    Abstract: Background: Deep neuromuscular block (NMB) has been shown to improve surgical conditions and alleviate post-operative pain in bariatric surgery compared with moderate NMB. We hypothesized that deep NMB could also improve the quality of early recovery ... ...

    Abstract Background: Deep neuromuscular block (NMB) has been shown to improve surgical conditions and alleviate post-operative pain in bariatric surgery compared with moderate NMB. We hypothesized that deep NMB could also improve the quality of early recovery after laparoscopic sleeve gastrectomy (LSG).
    Methods: Eighty patients were randomized to receive either deep (post-tetanic count 1-3) or moderate (train-of-four count 1-3) NMB. The QoR-15 questionnaire was used to evaluate the quality of early recovery at 1 day before surgery (T0), 24 and 48 h after surgery (T2, T3). Additionally, we recorded diaphragm excursion (DE), postoperative pain, surgical condition, cumulative dose of analgesics, time of first flatus and ambulation, post-operative nausea and vomiting, time of tracheal tube removal and hospitalization time.
    Main results: The quality of recovery was significantly better 24 h after surgery in patients who received a deep versus moderate block (114.4 ± 12.9 versus 102.1 ± 18.1). Diaphragm excursion was significantly greater in the deep NMB group when patients performed maximal inspiration at T2 and T3 (P < 0.05). Patients who underwent deep NMB reported lower visceral pain scores 40 min after surgery; additionally, these patients experienced lower pain during movement at T3 (P < 0.05). Optimal surgical conditions were rated in 87.5% and 64.6% of all measurements during deep and moderate NMB respectively (P < 0.001). The time to tracheal tube removal was significantly longer in the deep NMB group (P = 0.001). There were no differences in other outcomes.
    Conclusion: In obese patients receiving deep NMB during LSG, we observed improved QoR-15 scores, greater diaphragmatic excursions, improved surgical conditions, and visceral pain scores were lower. More evidence is needed to determine the effects of deep NMB on these outcomes.
    Trial registration: ChiCTR2200065919. Date of retrospectively registered: 18/11/2022.
    MeSH term(s) Humans ; Neuromuscular Blockade ; Visceral Pain ; Obesity ; Neuromuscular Diseases ; Pain, Postoperative/drug therapy ; Gastrectomy ; Laparoscopy
    Language English
    Publishing date 2024-03-16
    Publishing country England
    Document type Randomized Controlled Trial ; Journal Article
    ZDB-ID 2091252-3
    ISSN 1471-2253 ; 1471-2253
    ISSN (online) 1471-2253
    ISSN 1471-2253
    DOI 10.1186/s12871-024-02465-1
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  3. Article ; Online: Aquaporins and Neuropathic Pain.

    Wang, Fei-Xiang / Xu, Wen-Mei / Xu, Chi-Liang / Li, Jiang / Lin, Jing-Yan

    Frontiers in bioscience (Landmark edition)

    2022  Volume 28, Issue 2, Page(s) 35

    Abstract: Neuropathic pain is a chronic secondary pain condition resulting from lesions or diseases of the peripheral or central nervous system (CNS). Neuropathic pain is closely related to edema, inflammation, increased neuronal excitability, and central ... ...

    Abstract Neuropathic pain is a chronic secondary pain condition resulting from lesions or diseases of the peripheral or central nervous system (CNS). Neuropathic pain is closely related to edema, inflammation, increased neuronal excitability, and central sensitization caused by glutamate accumulation. Aquaporins (AQPs), mainly responsible for the transport and clearance of water and solute, play important roles in developing CNS diseases, especially neuropathic pain. This review focuses on the interaction of AQPs with neuropathic pain, and the potential of AQPs, especially aquaporins 4, as therapeutic targets.
    MeSH term(s) Humans ; Neuralgia ; Aquaporins ; Central Nervous System ; Glutamates ; Inflammation
    Chemical Substances Aquaporins ; Glutamates
    Language English
    Publishing date 2022-11-06
    Publishing country Singapore
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2704569-9
    ISSN 2768-6698 ; 2768-6698
    ISSN (online) 2768-6698
    ISSN 2768-6698
    DOI 10.31083/j.fbl2802035
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Halorubrum rutilum sp. nov. isolated from a marine solar saltern.

    Yin, Shuai / Wang, Zhao / Xu, Jia-Qi / Xu, Wen-Mei / Yuan, Pan-Pan / Cui, Heng-Lin

    Archives of microbiology

    2015  Volume 197, Issue 10, Page(s) 1159–1164

    Abstract: A halophilic archaeal strain, YJ-18-S1(T), was isolated from Yangjiang marine solar saltern, Guangxi Province, China. Cells were pleomorphic, stained Gram-negative and formed red-pigmented colonies on agar plates. Strain YJ-18-S1(T) was able to grow at ... ...

    Abstract A halophilic archaeal strain, YJ-18-S1(T), was isolated from Yangjiang marine solar saltern, Guangxi Province, China. Cells were pleomorphic, stained Gram-negative and formed red-pigmented colonies on agar plates. Strain YJ-18-S1(T) was able to grow at 20-55 °C (optimum 37 °C), at 0.9-4.8 M NaCl (optimum 2.6 M NaCl), at 0.005-1.0 M MgCl2 (optimum 0.3 MgCl2) and at pH 5.5-8.5 (optimum pH 7.0). The cells were lysed in distilled water, and the minimal NaCl concentration to prevent cell lysis was found to be 5 % (w/v). The major polar lipids of the strain were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and sulfated mannosyl glucosyl diether. The 16S rRNA gene and rpoB' gene of strain YJ-18-S1(T) were phylogenetically related to the corresponding genes of Halorubrum members (94.3-98.0 and 86.7-96.1 % similarities, respectively). The DNA G+C content of strain YJ-18-S1(T) was 66.2 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain YJ-18-S1(T) (=CGMCC 1.12554(T) = JCM 30030(T)) represents a new species of Halorubrum, for which the name Halorubrum rutilum sp. nov. is proposed.
    MeSH term(s) Base Composition/genetics ; China ; DNA, Archaeal/genetics ; Halorubrum/classification ; Halorubrum/genetics ; Halorubrum/isolation & purification ; Phospholipids/genetics ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Seawater/microbiology ; Sodium Chloride/metabolism
    Chemical Substances DNA, Archaeal ; PGP-Me ; Phospholipids ; RNA, Ribosomal, 16S ; Sodium Chloride (451W47IQ8X)
    Language English
    Publishing date 2015-12
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 124824-8
    ISSN 1432-072X ; 0302-8933
    ISSN (online) 1432-072X
    ISSN 0302-8933
    DOI 10.1007/s00203-015-1159-3
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  5. Article ; Online: Salinigranum salinum sp. nov., isolated from a marine solar saltern.

    Wang, Zhao / Xu, Jia-Qi / Xu, Wen-Mei / Li, Yang / Zhou, Yao / Lü, Zhen-Zhen / Hou, Jing / Zhu, Lin / Cui, Heng-Lin

    International journal of systematic and evolutionary microbiology

    2016  Volume 66, Issue 8, Page(s) 3017–3021

    Abstract: An extremely halophilic archaeal strain YJ-50-S2T was isolated from Yangjiang marine solar saltern, China. Cells were pleomorphic, stained Gram-negative and formed red-pigmented colonies on agar plates. Strain YJ-50-S2T was able to grow at 25-50 °C ( ... ...

    Abstract An extremely halophilic archaeal strain YJ-50-S2T was isolated from Yangjiang marine solar saltern, China. Cells were pleomorphic, stained Gram-negative and formed red-pigmented colonies on agar plates. Strain YJ-50-S2T was able to grow at 25-50 °C (optimum 37 °C), with 0.9-4.8 M NaCl (optimum 2.6 M NaCl) and 0-1.0 M MgCl2 (optimum 0.03 M MgCl2), and at pH 5.0-9.5 (optimum pH 7.5). The cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 5 % (w/v). The 16S rRNA gene and rpoB' gene of strain YJ-50-S2T were phylogenetically related to the corresponding genes of Salinigranum rubrum GX10T (97.0 % and 90.5 % similarities, respectively). The major polar lipids of strainYJ-50-S2T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, and two major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether and mannosyl glucosyl diether, respectively. Several unidentified glycolipids were also detected. The DNA G+C content of strain YJ-50-S2T was 65.2 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain YJ-50-S2Trepresents a novel species of the genus Salinigranum, for which the name Salinigranum salinum sp. nov. is proposed. The type strain is YJ-50-S2T (=CGMCC 1.12572T=JCM 30033T).
    MeSH term(s) Base Composition ; China ; DNA, Archaeal/genetics ; Euryarchaeota/classification ; Euryarchaeota/genetics ; Euryarchaeota/isolation & purification ; Genes, Archaeal ; Glycolipids/chemistry ; Phospholipids/chemistry ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Salinity ; Sequence Analysis, DNA ; Water Microbiology
    Chemical Substances DNA, Archaeal ; Glycolipids ; Phospholipids ; RNA, Ribosomal, 16S
    Language English
    Publishing date 2016-05-04
    Publishing country England
    Document type Journal Article
    ZDB-ID 2002336-4
    ISSN 1466-5034 ; 1466-5026
    ISSN (online) 1466-5034
    ISSN 1466-5026
    DOI 10.1099/ijsem.0.001138
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  6. Article ; Online: Halomarina salina sp. nov., isolated from a marine solar saltern.

    Xu, Wen-Mei / Xu, Jia-Qi / Zhou, Yao / Li, Yang / Lü, Zhen-Zhen / Hou, Jing / Zhu, Lin / Cui, Heng-Lin

    Antonie van Leeuwenhoek

    2016  Volume 109, Issue 8, Page(s) 1121–1126

    Abstract: A halophilic archaeal strain, designated ZS-57-S(T), was isolated from Zhoushan marine solar saltern, China. Cells were observed to be pleomorphic, stained Gram-negative and formed red pigmented colonies on agar plates. Optimal growth was obtained at 3.9  ...

    Abstract A halophilic archaeal strain, designated ZS-57-S(T), was isolated from Zhoushan marine solar saltern, China. Cells were observed to be pleomorphic, stained Gram-negative and formed red pigmented colonies on agar plates. Optimal growth was obtained at 3.9 M NaCl (range 1.4-4.8 M), 0.3 M MgCl2 (range 0-1.0 M), 30 °C (range 20-55 °C) and pH 6.5-7.5 (range 5.5-9.0). The cells were found to lyse in distilled water and the minimal NaCl concentration to prevent cell lysis was determined to be 5 % (w/v). The major polar lipids were identified as C20C20 and C20C25 diether derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, glucosyl mannosyl glucosyl diether and two unidentified glycolipids. The 16S rRNA gene and rpoB' gene of strain ZS-57-S(T) were phylogenetically related to the corresponding genes of Halomarina oriensis JCM 16495(T) (98.2 and 93.7 % similarities, respectively). The DNA G+C content of strain ZS-57-S(T) was determined to be 67.1 mol% (T m). The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain ZS-57-S(T) (=CGMCC 1.12543(T) = JCM 30039(T)) represents a new species of the genus Halomarina, for which the name Halomarina salina sp. nov. is proposed.
    MeSH term(s) China ; Genome, Archaeal ; Halobacteriaceae/classification ; Halobacteriaceae/genetics ; Halobacteriaceae/isolation & purification ; Halobacteriaceae/metabolism ; Phospholipids/metabolism ; Phylogeny ; Pigments, Biological/metabolism ; Salinity ; Seawater/microbiology ; Sequence Analysis, DNA
    Chemical Substances Phospholipids ; Pigments, Biological
    Language English
    Publishing date 2016-08
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 214861-4
    ISSN 1572-9699 ; 0003-6072
    ISSN (online) 1572-9699
    ISSN 0003-6072
    DOI 10.1007/s10482-016-0714-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Halorussus salinus sp. nov., isolated from a marine solar saltern.

    Xu, Jia-Qi / Xu, Wen-Mei / Li, Yang / Zhou, Yao / Lü, Zhen-Zhen / Hou, Jing / Zhu, Lin / Cui, Heng-Lin

    Archives of microbiology

    2016  Volume 198, Issue 10, Page(s) 957–961

    Abstract: A halophilic archaeal strain YJ-37- ... ...

    Abstract A halophilic archaeal strain YJ-37-H
    MeSH term(s) Base Composition ; China ; DNA, Archaeal/genetics ; DNA-Directed RNA Polymerases/genetics ; Glycolipids/analysis ; Halobacteriaceae/classification ; Halobacteriaceae/genetics ; Halobacteriaceae/isolation & purification ; Phospholipids/analysis ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Sodium Chloride/analysis
    Chemical Substances DNA, Archaeal ; Glycolipids ; PGP-Me ; Phospholipids ; RNA, Ribosomal, 16S ; S-TGD-1 ; Sodium Chloride (451W47IQ8X) ; DNA-Directed RNA Polymerases (EC 2.7.7.6)
    Language English
    Publishing date 2016-12
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 124824-8
    ISSN 1432-072X ; 0302-8933
    ISSN (online) 1432-072X
    ISSN 0302-8933
    DOI 10.1007/s00203-016-1253-1
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  8. Article: Halomarina salina sp. nov., isolated from a marine solar saltern

    Xu, Wen-Mei / Heng-Lin Cui / Jia-Qi Xu / Jing Hou / Lin Zhu / Yang Li / Yao Zhou / Zhen-Zhen Lü

    Antonie van Leeuwenhoek. 2016 Aug., v. 109, no. 8

    2016  

    Abstract: A halophilic archaeal strain, designated ZS-57-Sᵀ, was isolated from Zhoushan marine solar saltern, China. Cells were observed to be pleomorphic, stained Gram-negative and formed red pigmented colonies on agar plates. Optimal growth was obtained at 3 ... ...

    Abstract A halophilic archaeal strain, designated ZS-57-Sᵀ, was isolated from Zhoushan marine solar saltern, China. Cells were observed to be pleomorphic, stained Gram-negative and formed red pigmented colonies on agar plates. Optimal growth was obtained at 3.9 M NaCl (range 1.4–4.8 M), 0.3 M MgCl₂ (range 0–1.0 M), 30 °C (range 20–55 °C) and pH 6.5–7.5 (range 5.5–9.0). The cells were found to lyse in distilled water and the minimal NaCl concentration to prevent cell lysis was determined to be 5 % (w/v). The major polar lipids were identified as C₂₀C₂₀ and C₂₀C₂₅ diether derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, glucosyl mannosyl glucosyl diether and two unidentified glycolipids. The 16S rRNA gene and rpoB′ gene of strain ZS-57-Sᵀ were phylogenetically related to the corresponding genes of Halomarina oriensis JCM 16495ᵀ (98.2 and 93.7 % similarities, respectively). The DNA G+C content of strain ZS-57-Sᵀ was determined to be 67.1 mol% (T ₘ). The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain ZS-57-Sᵀ (=CGMCC 1.12543ᵀ = JCM 30039ᵀ) represents a new species of the genus Halomarina, for which the name Halomarina salina sp. nov. is proposed.
    Keywords agar ; chemotaxonomy ; DNA ; genes ; glycolipids ; magnesium chloride ; new species ; pH ; phenotype ; phosphates ; phylogeny ; ribosomal RNA ; sodium chloride ; sulfates ; China
    Language English
    Dates of publication 2016-08
    Size p. 1121-1126.
    Publishing place Springer International Publishing
    Document type Article
    ZDB-ID 214861-4
    ISSN 1572-9699 ; 0003-6072
    ISSN (online) 1572-9699
    ISSN 0003-6072
    DOI 10.1007/s10482-016-0714-5
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  9. Article ; Online: Halosimplex litoreum sp. nov., isolated from a marine solar saltern.

    Yuan, Pan-Pan / Xu, Jia-Qi / Xu, Wen-Mei / Wang, Zhao / Yin, Shuai / Han, Dong / Zhang, Wen-Jiao / Cui, Heng-Lin

    Antonie van Leeuwenhoek

    2015  Volume 108, Issue 2, Page(s) 483–489

    Abstract: A halophilic archaeal strain, YGH94(T), was isolated from the Yinggehai marine solar saltern near the Shanya city of Hainan Province, China. Cells of the strain were observed to be short rods, stain Gram-negative and to form red-pigmented colonies on ... ...

    Abstract A halophilic archaeal strain, YGH94(T), was isolated from the Yinggehai marine solar saltern near the Shanya city of Hainan Province, China. Cells of the strain were observed to be short rods, stain Gram-negative and to form red-pigmented colonies on solid media. Strain YGH94(T) was found to grow at 25-50 °C (optimum 40 °C), at 0.9-4.8 M NaCl (optimum 3.1 M), at 0-1.0 M MgCl2 (optimum 0.05 M) and at pH 5.0-9.0 (optimum pH 7.5). The cells were found to lyse in distilled water and the minimal NaCl concentration to prevent cell-lysis was determined to be 5 % (w/v). The major polar lipids were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and four major glycolipids (disulfated mannosyl glucosyl diether, sulfated mannosyl glucosyl diether and two unidentified glycolipids chromatographically identical to glycolipids in Halosimplex carlsbadense JCM 11222(T)). Strain YGH94(T) was found to possess two heterogeneous 16S rRNA genes (rrnA and rrnB) and both are related to those of Hsx. carlsbadense JCM 11222(T) (92.7-98.6 % similarities), Halosimplex pelagicum R2(T) (94.6-99.2 % similarities) and Halosimplex rubrum R27(T) (92.9-98.8 % similarities). The rpoB' gene similarity between strain YGH94(T) and Hsx. carlsbadense JCM 11222(T), Hsx. pelagicum R2(T) and Hsx. rubrum R27(T) are 95.4, 94.9 and 95.1 %, respectively. The DNA G+C content of strain YGH94(T) was determined to be 64.0 mol%. Strain YGH94(T) showed low DNA-DNA relatedness (35-39 %) with the current three members of the genus Halosimplex. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain YGH94(T) (=CGMCC 1.12235(T) = JCM 18647(T)) represents a new species of the genus Halosimplex, for which the name Halosimplex litoreum sp. nov. is proposed.
    MeSH term(s) Bacterial Typing Techniques ; Base Composition ; China ; Cluster Analysis ; Culture Media/chemistry ; Cytosol/chemistry ; DNA, Archaeal/chemistry ; DNA, Archaeal/genetics ; DNA, Ribosomal/chemistry ; DNA, Ribosomal/genetics ; DNA-Directed RNA Polymerases/genetics ; Environmental Microbiology ; Glycolipids/analysis ; Halobacteriaceae/classification ; Halobacteriaceae/genetics ; Halobacteriaceae/isolation & purification ; Halobacteriaceae/physiology ; Hydrogen-Ion Concentration ; Magnesium Chloride/metabolism ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Phospholipids/analysis ; Phylogeny ; Pigments, Biological/analysis ; RNA, Ribosomal, 16S/genetics ; Sequence Analysis, DNA ; Sodium Chloride/metabolism ; Temperature
    Chemical Substances Culture Media ; DNA, Archaeal ; DNA, Ribosomal ; Glycolipids ; Phospholipids ; Pigments, Biological ; RNA, Ribosomal, 16S ; Magnesium Chloride (02F3473H9O) ; Sodium Chloride (451W47IQ8X) ; DNA-Directed RNA Polymerases (EC 2.7.7.6) ; RNA polymerase beta subunit (EC 2.7.7.6)
    Language English
    Publishing date 2015-08
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 214861-4
    ISSN 1572-9699 ; 0003-6072
    ISSN (online) 1572-9699
    ISSN 0003-6072
    DOI 10.1007/s10482-015-0501-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Screening endophytic bacteria strains of anti Botrytis cinerea from the garlic bulb and their control effect

    Deng Zhenshan, Yan’an University, Yan’an (China), The Life-science Department / Ma Nana, Yan’an University, Yan’an (China), The Life-science Department / Xu Wenmei, Yan’an University, Yan’an (China), The Life-science Department

    Journal of Northwest Agriculture and Forestry University

    May. 2012  , Issue (5)

    Abstract: 筛选拮抗番茄灰霉病的内生菌菌株，研究其生防效果，为开发有应用潜力的微生物农药奠定基础。用平板对峙法从大蒜鳞茎内筛选具有拮抗作用的内生菌，测定拮抗菌株对番茄离体叶片、果实、胚根和盆栽植株灰霉病的防治效果，以及对7种常见病原真菌的抑菌活性。从分离纯化的63株候选菌株中获得了22株具有一定抑菌活性的内生菌，其中有4株内生菌(D6,D10,D21,Y3)对番茄灰霉病有显著的抑制效果，抑菌率最高可达77.5%。4株拮抗菌均有较强的抑菌活性，对番茄离体叶片、果实和胚根上番茄灰霉病菌的生长抑制率最高分别达92%，71. ...

    Abstract 筛选拮抗番茄灰霉病的内生菌菌株，研究其生防效果，为开发有应用潜力的微生物农药奠定基础。用平板对峙法从大蒜鳞茎内筛选具有拮抗作用的内生菌，测定拮抗菌株对番茄离体叶片、果实、胚根和盆栽植株灰霉病的防治效果，以及对7种常见病原真菌的抑菌活性。从分离纯化的63株候选菌株中获得了22株具有一定抑菌活性的内生菌，其中有4株内生菌(D6,D10,D21,Y3)对番茄灰霉病有显著的抑制效果，抑菌率最高可达77.5%。4株拮抗菌均有较强的抑菌活性，对番茄离体叶片、果实和胚根上番茄灰霉病菌的生长抑制率最高分别达92%，71.13%和70.3%；对盆栽植株番茄灰霉病菌的抑制率最高可达96.2%；这4株菌对西瓜枯萎病菌(Fusarium oxysporum f.sp.niveum)、玉米弯孢病菌(Curvularia lunata)、烟草赤星病菌(Alternaria longipes)、小麦赤霉病菌(Fusarium graminearum)、苹果炭疽病菌(Glomerella cingulata)、稻瘟病菌(Pyricularia oryzae Cav.)和棉花枯萎病菌(Fusarium oxysporun f.sp.vasinfectum)均有一定的抑菌活性，抑制率最高达83.1%。大蒜鳞茎中的内生菌对番茄灰霉病有明显的抑制作用，这为内生菌在番茄灰霉病防治方面的应用提供了理论依据。
    Keywords GARLIC ; BOTRYTIS CINEREA ; BACTERIA ; AIL ; BOTRYTIS CINEREA ; BACTERIA ; AJO ; BOTRYTIS CINEREA ; BACTERIA ; http://www.fao.org/aos/agrovoc#c_11091 ; http://www.fao.org/aos/agrovoc#c_26413 ; http://www.fao.org/aos/agrovoc#c_765
    Language zho
    Document type Article
    ISSN 1671-9387
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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