LIVIVO - Search results -

Search results

Result 1 - 10 of total 82

Search options

Article: Identification and Functional Analysis of

Li, Lu / Li, Xihong / Chen, Yadong / Yang, Yingming / Wang, Na / Xu, Wenteng

Animals : an open access journal from MDPI

2024 Volume 14, Issue 2

Abstract: The high proportion of males ... ...

Abstract	The high proportion of males in
Language	English
Publishing date	2024-01-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606558-7
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani14020311
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article: Three Copies of

Sun, Yuqi / Li, Xihong / Mai, Jiaqi / Xu, Wenteng / Wang, Jiacheng / Zhang, Qi / Wang, Na

Biology

2024 Volume 13, Issue 3

Abstract: The sex chromosome, especially specific in one sex, generally determines sexual size dimorphism (SSD), a phenomenon with dimorphic sexual difference in the body size. ... ...

Abstract	The sex chromosome, especially specific in one sex, generally determines sexual size dimorphism (SSD), a phenomenon with dimorphic sexual difference in the body size. For
Language	English
Publishing date	2024-02-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2661517-4
ISSN	2079-7737
ISSN	2079-7737
DOI	10.3390/biology13030141
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Phosphoproteomics Reveal New Candidates in Abnormal Spermatogenesis of Pseudomales in

Li, Xihong / Li, Lu / Cui, Zhongkai / Li, Ming / Xu, Wenteng

International journal of molecular sciences

2023 Volume 24, Issue 14

Abstract: Phosphorylation is a post-translational modification that contributes to versatile protein functions in spermatogenesis, and the variations they generate usually results in abnormal spermatogenesis or sperm dysfunction. The sex-reversal phenomenon exists ...

Abstract	Phosphorylation is a post-translational modification that contributes to versatile protein functions in spermatogenesis, and the variations they generate usually results in abnormal spermatogenesis or sperm dysfunction. The sex-reversal phenomenon exists in Chinese tongue sole under certain conditions such that individuals with a ZW genotype can acquire a male phenotype and are thus called pseudomales. Pseudomale tongue sole can reach sexual maturity but produce only Z-type sperm, and the Z sperm carries paternal epigenetic information. Whether phosphorylation plays a role in the sperm abnormality of pseudomales is unknown. In this study, a phosphoproteomic analysis was performed to compare protein phosphorylation profiles between pseudomale and male testes. Altogether, we identified 14,253 phosphopeptides matching with 4843 proteins, with 1329 differentially phosphorylated peptides corresponding to 1045 differentially phosphorylated proteins (DPPs). Phosphorylation at 781 sites was upregulated and at 548 sites was downregulated. Four motifs were identified among differentially phosphorylated peptides, which were "SP", "SD", "RxxS", and "TP". Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses suggested that the cell cycle and DNA/RNA processing were significantly enriched with the genes encoding DPPs. To analyze DPP function in depth, a protein-protein interaction network was constructed, and Ran-binding protein 2 was found to play a central role in spermatogenesis by regulating several processes such as the cell cycle, eukaryotic translation, ubiquitination, and minichromosome maintenance. In kinase-associated network analyses, two "mitogen-activated protein kinase (Mapk)-centered" clusters were identified that may account for abnormal spermatogenesis in pseudomales. One cluster was centered on Mapk6, which predominantly regulated the cell cycle by interacting with several cyclin-dependent kinases, and the other was centered on the "testis-expressed kinase 1-like (Tesk1l)/Pim1l-Mapk4l- testis-expressed 14 (Tex14)" kinase cascade, which might contribute to spermatogenesis by regulating β-catenin. Taken together, these data suggested the new candidates involved in pseudomale sperm abnormalities and provided clues to discover the phosphorylated regulatory mechanism underlying tongue sole spermatogenesis.
MeSH term(s)	Male ; Animals ; Semen ; Testis/metabolism ; Spermatogenesis/genetics ; Spermatozoa ; Fishes
Language	English
Publishing date	2023-07-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms241411430
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: A patched1 gene homologue participates in female differentiation of Cynoglossus semilaevis.

Qi, Qian / Dong, Zhongdian / Zhang, Ning / Shao, Changwei / Xu, Wenteng

Gene expression patterns : GEP

2022 Volume 45, Page(s) 119265

Abstract: Patched (Ptch) is a receptor in the hedgehog signaling pathway, essential for animal development. Our previous study showed that ptch1 gene participates in the maintenance of the male germline and spermatogenesis in Cynoglossus semilaevis (csptch1). In ... ...

Abstract	Patched (Ptch) is a receptor in the hedgehog signaling pathway, essential for animal development. Our previous study showed that ptch1 gene participates in the maintenance of the male germline and spermatogenesis in Cynoglossus semilaevis (csptch1). In this study, we identified a patched1 gene homolog (csptch1 x1). The csptch1 x1 gene is 5761 bp long, with a 4638 bp coding sequence that encodes 1545 amino acids. The Csptch1 x1 protein has 12 transmembrane regions and sterol-sensing domains and is highly homologous to the csptch1 (91 amino acids difference). Expression pattern analysis showed that csptch1 x1 is expressed in eight different tissues of adult tongue sole, and the expression is significantly higher in tissues of female than that in male tissues. The expression pattern in developmental stages was also analyzed. csptch1 x1 could be detected at the 1-cell stage and was highly expressed at the blastocyst, somite, and blastopore closing stages, implying that it participates in cell differentiation. In ovarian development, the expression of csptch1 x1 was initiated at 20 days after hatching (dah) and was significantly high at 35-50 and 95-150 dah. In situ hybridization showed that csptch1 x1 was predominantly expressed in primordial germ cells, oocytes, and follicular cells, but the expression of the gene was lower in the testis. These results suggest that csptch1 x1 may be mainly involved in female differentiation and ovarian development, different from the role of csptch1 in spermatogenesis.
MeSH term(s)	Amino Acids/genetics ; Amino Acids/metabolism ; Animals ; Female ; Fish Proteins/metabolism ; Flatfishes/genetics ; Flounder ; Hedgehog Proteins/genetics ; Male ; Phylogeny
Chemical Substances	Amino Acids ; Fish Proteins ; Hedgehog Proteins
Language	English
Publishing date	2022-08-06
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2058346-1
ISSN	1872-7298 ; 1567-133X
ISSN (online)	1872-7298
ISSN	1567-133X
DOI	10.1016/j.gep.2022.119265
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 5515: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Sustainable use of CRISPR/Cas in fish aquaculture: the biosafety perspective

Okoli, Arinze S. / Blix, Torill / Myhr, Anne I. / Xu, Wenteng / Xu, Xiaodong

Transgenic research. 2022 Feb., v. 31, no. 1

2022

Abstract: Aquaculture is becoming the primary source of seafood for human diets, and farmed fish aquaculture is one of its fastest growing sectors. The industry currently faces several challenges including infectious and parasitic diseases, reduced viability, ... ...

Abstract	Aquaculture is becoming the primary source of seafood for human diets, and farmed fish aquaculture is one of its fastest growing sectors. The industry currently faces several challenges including infectious and parasitic diseases, reduced viability, fertility reduction, slow growth, escapee fish and environmental pollution. The commercialization of the growth-enhanced AquAdvantage salmon and the CRISPR/Cas9-developed tilapia (Oreochromis niloticus) proffers genetic engineering and genome editing tools, e.g. CRISPR/Cas, as potential solutions to these challenges. Future traits being developed in different fish species include disease resistance, sterility, and enhanced growth. Despite these notable advances, off-target effect and non-clarification of trait-related genes among other technical challenges hinder full realization of CRISPR/Cas potentials in fish breeding. In addition, current regulatory and risk assessment frameworks are not fit-for purpose regarding the challenges of CRISPR/Cas notwithstanding that public and regulatory acceptance are key to commercialization of products of the new technology. In this study, we discuss how CRISPR/Cas can be used to overcome some of these limitations focusing on diseases and environmental release in farmed fish aquaculture. We further present technical limitations, regulatory and risk assessment challenges of the use of CRISPR/Cas, and proffer research strategies that will provide much-needed data for regulatory decisions, risk assessments, increased public awareness and sustainable applications of CRISPR/Cas in fish aquaculture with emphasis on Atlantic salmon (Salmo salar) breeding.
Keywords	Oreochromis niloticus ; Salmo salar ; biosafety ; commercialization ; disease resistance ; farmed fish ; fish culture ; humans ; industry ; pollution ; risk ; salmon ; seafoods ; technology ; viability
Language	English
Dates of publication	2022-02
Size	p. 1-21.
Publishing place	Springer International Publishing
Document type	Article
Note	Review
ZDB-ID	31620-9
ISSN	1573-9368 ; 0962-8819
ISSN (online)	1573-9368
ISSN	0962-8819
DOI	10.1007/s11248-021-00274-7
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 3600: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: A patched1 gene homologue participates in female differentiation of Cynoglossus semilaevis

Qi, Qian / Dong, Zhongdian / Zhang, Ning / Shao, Changwei / Xu, Wenteng

Gene expression patterns. 2022 Sept., v. 45

2022

Abstract	Patched (Ptch) is a receptor in the hedgehog signaling pathway, essential for animal development. Our previous study showed that ptch1 gene participates in the maintenance of the male germline and spermatogenesis in Cynoglossus semilaevis (csptch1). In this study, we identified a patched1 gene homolog (csptch1 x1). The csptch1 x1 gene is 5761 bp long, with a 4638 bp coding sequence that encodes 1545 amino acids. The Csptch1 x1 protein has 12 transmembrane regions and sterol-sensing domains and is highly homologous to the csptch1 (91 amino acids difference). Expression pattern analysis showed that csptch1 x1 is expressed in eight different tissues of adult tongue sole, and the expression is significantly higher in tissues of female than that in male tissues. The expression pattern in developmental stages was also analyzed. csptch1 x1 could be detected at the 1-cell stage and was highly expressed at the blastocyst, somite, and blastopore closing stages, implying that it participates in cell differentiation. In ovarian development, the expression of csptch1 x1 was initiated at 20 days after hatching (dah) and was significantly high at 35–50 and 95–150 dah. In situ hybridization showed that csptch1 x1 was predominantly expressed in primordial germ cells, oocytes, and follicular cells, but the expression of the gene was lower in the testis. These results suggest that csptch1 x1 may be mainly involved in female differentiation and ovarian development, different from the role of csptch1 in spermatogenesis.
Keywords	Cynoglossus semilaevis ; Erinaceidae ; adults ; blastocyst ; cell differentiation ; females ; gene expression ; genes ; hybridization ; males ; oocytes ; ovarian development ; spermatogenesis ; testes
Language	English
Dates of publication	2022-09
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	2058346-1
ISSN	1872-7298 ; 1567-133X
ISSN (online)	1872-7298
ISSN	1567-133X
DOI	10.1016/j.gep.2022.119265
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 5515: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Identification of stress-related genes by co-expression network analysis based on the improved turbot genome.

Xu, Xi-Wen / Zheng, Weiwei / Meng, Zhen / Xu, Wenteng / Liu, Yingjie / Chen, Songlin

Scientific data

2022 Volume 9, Issue 1, Page(s) 374

Abstract: Turbot (Scophthalmus maximus), commercially important flatfish species, is widely cultivated in Europe and China. With the continuous expansion of the intensive breeding scale, turbot is exposed to various stresses, which greatly impedes the healthy ... ...

Abstract	Turbot (Scophthalmus maximus), commercially important flatfish species, is widely cultivated in Europe and China. With the continuous expansion of the intensive breeding scale, turbot is exposed to various stresses, which greatly impedes the healthy development of turbot industry. Here, we present an improved high-quality chromosome-scale genome assembly of turbot using a combination of PacBio long-read and Illumina short-read sequencing technologies. The genome assembly spans 538.22 Mb comprising 27 contigs with a contig N50 size of 25.76 Mb. Annotation of the genome assembly identified 104.45 Mb repetitive sequences, 22,442 protein-coding genes and 3,345 ncRNAs. Moreover, a total of 345 stress responsive candidate genes were identified by gene co-expression network analysis based on 14 published stress-related RNA-seq datasets consisting of 165 samples. Significantly improved genome assembly and stress-related candidate gene pool will provide valuable resources for further research on turbot functional genome and stress response mechanism, as well as theoretical support for the development of molecular breeding technology for resistant turbot varieties.
MeSH term(s)	Animals ; Flatfishes/genetics ; Gene Expression Regulation ; Genome ; Genomics ; High-Throughput Nucleotide Sequencing ; Molecular Sequence Annotation ; Sequence Analysis, DNA ; Stress, Physiological/genetics
Language	English
Publishing date	2022-06-29
Publishing country	England
Document type	Dataset ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2775191-0
ISSN	2052-4463 ; 2052-4463
ISSN (online)	2052-4463
ISSN	2052-4463
DOI	10.1038/s41597-022-01458-4
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Identification and Functional Analysis of

Zhang, Tingting / Zhang, Mengqian / Sun, Yuxuan / Li, Lu / Cheng, Peng / Li, Xihong / Wang, Na / Chen, Songlin / Xu, Wenteng

International journal of molecular sciences

2023 Volume 24, Issue 8

Abstract: The Chinese tongue sole ( ...

Abstract	The Chinese tongue sole (
MeSH term(s)	Animals ; Female ; Male ; Phylogeny ; Flatfishes/genetics ; Flatfishes/metabolism ; Fish Proteins/genetics ; Fish Proteins/metabolism ; Amino Acid Sequence ; Testis/metabolism ; Flounder/genetics
Chemical Substances	Fish Proteins
Language	English
Publishing date	2023-04-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24087625
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Genome-wide identification, immune response profile and functional characterization of IL-10 from spotted knifejaw (Oplegnathus punctatus) during host defense against bacterial and viral infection

Wang, Jie / Chen, Zhangfan / Li, Ming / Ziwei zhang / Song, Yu / Xu, Wenteng / Wang, Lei / Chen, Songlin

Fish & shellfish immunology. 2022 May, v. 124

2022

Abstract: Interleukin 10 (IL-10), a pleiotropic cytokine, plays an essential role in multiple immunity responses. In the current study, the sequences of IL-10 family were identified from spotted knifejaw (Oplegnathus punctatus) whole genome, and O. punctatus IL-10 ...

Abstract	Interleukin 10 (IL-10), a pleiotropic cytokine, plays an essential role in multiple immunity responses. In the current study, the sequences of IL-10 family were identified from spotted knifejaw (Oplegnathus punctatus) whole genome, and O. punctatus IL-10 (OpIL-10) was cloned and characterized. OpIL-10 encodes 187 amino acids with a typical IL-10 family signature motif and predicted α-helices. It shared high identities with Notolabrus celidotus IL-10 and Epinephelus Lanceolatus IL-10. OpIL-10 was widely detected in healthy tissues, with the abundant expression in liver and skin. It was significantly up-regulated in the six immune-related tissues (liver, spleen, kidney, intestine, gill and skin) after infection against Vibrio harveyi and spotted knifejaw iridovirus (SKIV). Dual-luciferase analysis showed that OpIL-10 overexpression could suppress the activity of NF-κB. Meanwhile, OpIL-10 knockdown caused the down-regulation of five immune-related genes in JAK2/STAT3 signaling pathway and NF-κB signaling pathway, including IL-10R2, TYK2, STAT3, NOD2, and IκB. In addition, LPS and poly I:C stimulated expression of pro-inflammatory cytokines, including IL-6, IL-1β, IL-8, and IL-12, were lower with recombinant OpIL-10 (rOp IL-10) than the control group, indicating the anti-inflammatory roles of rOpIL-10. Taken together, these results indicated OpIL-10 as a negative regulator in the inflammatory responses of spotted knifejaw against bacterial and viral infection, which would help us better understand the role of IL-10 in teleost immunity.
Keywords	Epinephelus lanceolatus ; Iridovirus ; Notolabrus ; Oplegnathus ; Vibrio harveyi ; fish ; immune response ; interleukin-10 ; interleukin-12 ; interleukin-6 ; interleukin-8 ; intestines ; intracellular signaling peptides and proteins ; kidneys ; liver ; polyinosinic-polycytidylic acid ; shellfish ; spleen
Language	English
Dates of publication	2022-05
Size	p. 513-524.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	1067738-0
ISSN	1095-9947 ; 1050-4648
ISSN (online)	1095-9947
ISSN	1050-4648
DOI	10.1016/j.fsi.2022.04.026
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 5917: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Transcriptomic profiles of spotted knifejaw (Oplegnathus punctatus) spleen in response to Megalocytivirus infection

Xu, Wenteng / Li, Ming / Wang, Shan / Song, Yu / Xu, Hao / Lin, Kai / Wang, Jie / Chen, Songlin

Aquaculture. 2022 June, v. 555 p.738212-

2022

Abstract: Megalocytivirus (MCV) is emerging as a major viral pathogen in aquaculture and has caused substantial economic losses due to its wide infectious spectrum. As the spotted knifejaw (Oplegnathus punctatus) is one of the most susceptible fish to MCV, the MCV ...

Abstract	Megalocytivirus (MCV) is emerging as a major viral pathogen in aquaculture and has caused substantial economic losses due to its wide infectious spectrum. As the spotted knifejaw (Oplegnathus punctatus) is one of the most susceptible fish to MCV, the MCV infection in spotted knifejaw caused massive mortality, therefore significantly hampers the farming industry. Despite of the significant economic importance, the immune response of spotted knifejaw during MCV infection is still unclear, which limits the breeding of resistant fish lines and becomes a research priority for aquaculture worldwide. In this study, we challenged spotted knifejaw with a spotted knifejaw MCV strain (SKIV-SD) and performed a transcriptomic analysis using spleen samples from five different time points (days 0, 1, 4, 7, and 10). Fifteen samples (three for each time point) were employed to construct the RNA libraries. A total of over 100 Gb of clean bases was generated, and the clean reads per library ranged from 39.37 M to 60.87 M. A total of 23,365 genes were annotated, and 32,542 new transcripts were identified. Through a comparative transcriptomic analysis of the five groups, the nucleotide binding oligomerization domain-like (NOD-like) receptor signalling pathway, haematopoietic cell lineage, and cytosolic DNA-sensing pathway were closely related to MCV challenge, and the gene expression levels in these pathways were validated by qPCR. Our data provide a genetic resource and shed light on the immune response to MCV infection in spotted knifejaw.
Keywords	Megalocytivirus ; Oplegnathus ; RNA ; aquaculture ; fish ; gene expression ; genetic resources ; immune response ; industry ; mortality ; oligomerization ; pathogens ; spleen ; transcriptomics ; Oplegnathus punctatus ; Transcriptome ; Immune-related pathway
Language	English
Dates of publication	2022-06
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	185380-6
ISSN	0044-8486 ; 0044-8516
ISSN	0044-8486 ; 0044-8516
DOI	10.1016/j.aquaculture.2022.738212
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 3275: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED