Article: Role of L-Arginine on the Expression of Coagulation Factor VIII Gene.
Alternative therapies in health and medicine
2023 Volume 29, Issue 3, Page(s) 26–31
Abstract: Objective: To explore the key sites in which L-arginine affects the expression of human coagulation factor VIII gene, and to create new drug targets for the treatment of hemophilia.: Methods: A total of 5 human FVIII genes (A1, A2, A3, C1 and C2) ... ...
Abstract | Objective: To explore the key sites in which L-arginine affects the expression of human coagulation factor VIII gene, and to create new drug targets for the treatment of hemophilia. Methods: A total of 5 human FVIII genes (A1, A2, A3, C1 and C2) with B domain deletion were transfected into human umbilical vein endothelial cells (HUVECs) as promoters. Run-on assay and ELISA analysis were performed to observe the driving effect of each domain gene on chloramphenicol acetyl transferase (CAT) gene transcription and expression, and the effect of L-arginine on each promoter. Results: In co-culture with L-arginine, transcriptional expression of the CAT gene was not detected in the PCAT3-Basic group (negative control without promoters), PA3-CAT3-Enhancer group or PC1-CAT3-Enhancer group. The transcriptional expression of CAT gene in the PCAT3-Control group (positive control with promoters) and PA1-CAT3-Enhancer group was unchanged compared with the non-L-arginine intervention, while the transcriptional expression of CAT gene in the PA2-CAT3-Enhancer group was significantly enhanced. Conclusions: A1 and A2 domain genes had promoter function and could initiate the transcription and expression of CAT gene, but A3, C1 and C2 domain genes could not. Moreover, L-arginine can significantly enhance transcription and expression of human coagulation factor VIII via A2 domain. |
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MeSH term(s) | Humans ; Factor VIII/genetics ; Factor VIII/metabolism ; Endothelial Cells/metabolism ; Arginine/pharmacology |
Chemical Substances | Factor VIII (9001-27-8) ; Arginine (94ZLA3W45F) |
Language | English |
Publishing date | 2023-02-03 |
Publishing country | United States |
Document type | Journal Article |
ZDB-ID | 1225073-9 |
ISSN | 1078-6791 |
ISSN | 1078-6791 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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