LIVIVO - Search results -

Search results

Result 1 - 10 of total 83

Search options

Article ; Online: Effects of miR-490-5p targeting CDK1 on proliferation and apoptosis of colon cancer cells via ERK signaling pathway.

Yang, Y-J / Luo, S / Xu, Z-L

European review for medical and pharmacological sciences

2022 Volume 26, Issue 6, Page(s) 2049–2056

Abstract: Objective: The aim of this study was to investigate the effects of micro ribonucleic acid (miR)-490-5p on the proliferation and apoptosis of colon cancer cells, and to explore its potential mechanism.: Patients and methods: The mRNA expression of miR- ...

Abstract	Objective: The aim of this study was to investigate the effects of micro ribonucleic acid (miR)-490-5p on the proliferation and apoptosis of colon cancer cells, and to explore its potential mechanism. Patients and methods: The mRNA expression of miR-490-5p in 30 pairs of colon cancer tissues and adjacent normal tissues was detected via reverse transcription-polymerase chain reaction (RT-PCR). Human colon cancer SW480 cell lines were cultured in vitro and divided into Control group and miR-490-5p overexpression group (miR-490-5p mimic group). The nonsense sequence and miR-490-5p mimic were transfected using liposome transfection technique into colon cancer cells in control group and miR-490-5p mimic group, respectively. Cell proliferation and apoptosis in each group were then observed. At the same time, the effect of miR-490-5p on the growth of colon cancer in vivo was explored using subcutaneous tumorigenesis assay. The protein expressions of extracellular signal-regulated kinase (ERK)1/2 signaling pathway and cyclin-dependent kinase 1 (CDK1) were determined via Western blotting. Furthermore, immunohistochemical staining was performed to verify the protein expression of CDK1 in vivo. Results: The expression of miR-490-5p in colon cancer tissues was significantly lower than that in adjacent normal tissues (p<0.05). After transfection with miR-490-5p mimic in vitro, EdU staining and colony formation assay showed that the proliferation ability of SW480 cells was significantly weakened (p<0.05). Meanwhile, the number of colonies in miR-490-5p mimic group was markedly less than that in Control group (p<0.05). The results of Western blotting revealed that overexpression of miR-490-5p remarkably up-regulated the Bax/Bcl-2 and C-Caspase3/T-Caspase3 ratios in cancer cells (p<0.05). Subsequent results indicated that the subcutaneous tumorigenesis of colon cancer cells was markedly inhibited by overexpression of miR-490-5p (p<0.05). According to the results of Western blotting, the activation of ERK signaling pathway and the protein expression of CDK1 were significantly suppressed by overexpression of miR-490-5p (p<0.05). In vivo experiments further revealed that the protein expression of CDK1 in colon cancer tissues increased significantly (p<0.05). Conclusions: MiR-490-5p was found lowly expressed in colon cancer patients. In addition, overexpression of miR-490-5p inhibited the proliferation and promoted the apoptosis of colon cancer cells via down-regulating CDK1 both in vitro and in vivo.
MeSH term(s)	Apoptosis/genetics ; CDC2 Protein Kinase/genetics ; CDC2 Protein Kinase/metabolism ; Cell Line, Tumor ; Cell Proliferation/genetics ; Colonic Neoplasms/genetics ; Humans ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Signal Transduction
Chemical Substances	MIRN490 microRNA, human ; MicroRNAs ; CDC2 Protein Kinase (EC 2.7.11.22) ; CDK1 protein, human (EC 2.7.11.22)
Language	English
Publishing date	2022-04-01
Publishing country	Italy
Document type	Journal Article
ZDB-ID	605550-3
ISSN	2284-0729 ; 1128-3602 ; 0392-291X
ISSN (online)	2284-0729
ISSN	1128-3602 ; 0392-291X
DOI	10.26355/eurrev_202203_28353
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1887: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: No causal effects between rosiglitazone and cardiovascular disease or risk factors: a Mendelian randomization study.

Li, X-M / Wu, Z-J / Xu, Z-L / Li, A / Liu, M-Q / Song, C-G / Wu, K

European review for medical and pharmacological sciences

2023 Volume 27, Issue 11, Page(s) 5280–5292

Abstract: Objective: Although many observational studies have shown an association between rosiglitazone and cardiovascular disease (CVD) or risk factors, controversy remains. We conducted a Mendelian randomized (MR) study to explore whether rosiglitazone is ... ...

Abstract	Objective: Although many observational studies have shown an association between rosiglitazone and cardiovascular disease (CVD) or risk factors, controversy remains. We conducted a Mendelian randomized (MR) study to explore whether rosiglitazone is causally related to CVDs and risk factors. Patients and methods: Single-nucleotide polymorphisms associated with rosiglitazone at genome-wide significance were identified from a genome-wide association study of 337,159 European-ancestry individuals. Four treatments with rosiglitazone-associated single-nucleotide polymorphisms associated with a higher risk of CVDs were used as an instrumental variable (IV). Summary-level data for 7 CVDs and 7 risk factors were obtained from UK Biobank and consortia. Results: We found no causal effects of rosiglitazone, either on CVDs or risk factors. The results were consistent in sensitivity analyses using Cochran's Q test, MR-PRESSO method, leave-one-out analysis and Mendelian randomization-Egger method (MR-Egger), and no directional pleiotropy was observed. Sensitivity analyses confirmed that rosiglitazone was not significantly associated with CVDs and risk factors. Conclusions: The findings from this MR study indicate no causal relationship between rosiglitazone and CVDs or risk factors. Hence, previous observational studies may have been biased.
MeSH term(s)	Humans ; Cardiovascular Diseases/chemically induced ; Cardiovascular Diseases/epidemiology ; Cardiovascular Diseases/genetics ; Genome-Wide Association Study ; Mendelian Randomization Analysis ; Risk Factors ; Polymorphism, Single Nucleotide ; Rosiglitazone/adverse effects
Chemical Substances	Rosiglitazone (05V02F2KDG)
Language	English
Publishing date	2023-06-15
Publishing country	Italy
Document type	Journal Article
ZDB-ID	605550-3
ISSN	2284-0729 ; 1128-3602 ; 0392-291X
ISSN (online)	2284-0729
ISSN	1128-3602 ; 0392-291X
DOI	10.26355/eurrev_202306_32647
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1887: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: [Impact of SARS-CoV-2 infection on graft composition and early transplant outcomes following allogeneic hematopoietic stem cell transplantation].

Lin, F / Sun, H / Chen, Y / Zhang, Y Y / Liu, J / He, Y / Zheng, F M / Xu, Z L / Wang, F R / Kong, J / Wang, Z D / Wan, Y Y / Mo, X D / Wang, Y / Cheng, Y F / Zhang, X H / Huang, X J / Xu, L P

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi

2024 Volume 44, Issue 11, Page(s) 890–899

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Humans ; COVID-19 ; SARS-CoV-2 ; Hematopoietic Stem Cell Transplantation ; Tissue Donors ; Graft vs Host Disease
Language	Chinese
Publishing date	2024-01-05
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	2997162-7
ISSN	0253-2727 ; 0253-2727
ISSN (online)	0253-2727
ISSN	0253-2727
DOI	10.3760/cma.j.issn.0253-2727.2023.11.002
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: [A study of clinical and genetic characteristics of a Leber hereditary optic neuropathy family with the heteroplasmic m.14484T>C mutation].

Sun, Y / Lei, K / Xu, Z L / Geng, Y

Zhonghua yan ke za zhi] Chinese journal of ophthalmology

2018 Volume 54, Issue 7, Page(s) 526–534

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Child, Preschool ; Cross-Sectional Studies ; DNA, Mitochondrial ; Evoked Potentials, Visual ; Female ; Humans ; Male ; Middle Aged ; Mutation ; Optic Atrophy, Hereditary, Leber/genetics ; Optic Atrophy, Hereditary, Leber/physiopathology ; Pedigree ; Phylogeny ; Young Adult
Chemical Substances	DNA, Mitochondrial
Language	Chinese
Publishing date	2018-07-11
Publishing country	China
Document type	Journal Article
ZDB-ID	604574-1
ISSN	0412-4081
ISSN	0412-4081
DOI	10.3760/cma.j.issn.0412-4081.2018.07.012
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 2482: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MO 267: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: [Epidemiological characteristics of pre-hospital mortality due to acute myocardial infarction from 1999 to 2016 in Tianjin city].

Jiang, G H / Wang, D Z / Zhang, H / Xue, X D / Pan, Y / Wang, C / Zhang, Y / Xu, Z L

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine

2020 Volume 54, Issue 1, Page(s) 99–103

Abstract: To explore the epidemiological characteristics, trends and relevant factors of pre-hospital mortality due to acute myocardial infarction (AMI) from 1999 to 2016 in Tianjin city, based on mortality surveillance information and household registration ... ...

Abstract	To explore the epidemiological characteristics, trends and relevant factors of pre-hospital mortality due to acute myocardial infarction (AMI) from 1999 to 2016 in Tianjin city, based on mortality surveillance information and household registration population information. Standardized mortality rates were calculated using the year 2000 world standard population. From 1999 to 2016, the research result showed that the pre-hospital crude mortality rates of AMI were 39.47/100 000 to 90.64/100 000 and the standardized mortality rates were 30.92/100 000 to 53.90/100 000. The proportion of pre-hospital AMI deaths was 73.96%-81.92% (
MeSH term(s)	Aged ; China/epidemiology ; Cities ; Female ; Humans ; Male ; Mortality/trends ; Myocardial Infarction/mortality ; Socioeconomic Factors
Language	Chinese
Publishing date	2020-01-07
Publishing country	China
Document type	Journal Article
ZDB-ID	604575-3
ISSN	0253-9624
ISSN	0253-9624
DOI	10.3760/cma.j.issn.0253-9624.2020.01.018
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 2487: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: The relationship between HSP60 gene polymorphisms and susceptibility to atherosclerosis.

Liao, B-H / Xu, Z-L / Gao, F / Zhang, S-H / Liang, R-J / Dong, S-H

European review for medical and pharmacological sciences

2020 Volume 24, Issue 5, Page(s) 2667–2673

Abstract: Objective: To explore the potential correlation between heat shock protein 60 (HSP60) gene polymorphisms and susceptibility to atherosclerosis.: Patients and methods: A total of 160 atherosclerosis patients treated in our hospital from February 2017 ... ...

Abstract	Objective: To explore the potential correlation between heat shock protein 60 (HSP60) gene polymorphisms and susceptibility to atherosclerosis. Patients and methods: A total of 160 atherosclerosis patients treated in our hospital from February 2017 to February 2019 were randomly enrolled as case group, and 200 healthy adults receiving physical examination were selected as control group at the same period. Venous blood was drawn from all subjects to extract deoxyribonucleic acid (DNA). TaqMan probe technology was employed to genotype two loci rs2340690 and rs788016 of HSP60 gene in all 260 subjects. The correlations between HSP60 gene polymorphisms and the incidence rate and pathological grade of atherosclerosis were analyzed. Results: There were three genotypes (AA, AG, and GG) in HSP60 rs2340690 and three (GG, AG, and AA) in HSP60 rs788016. No significant differences in the frequency of each genotype were found between the two groups (p>0.05). HSP60 rs2340690 and HSP60 rs788016 had no significant associations with the incidence rate of atherosclerosis in the dominant, recessive, and additive genetic models. In the case of pathological grade IV, the proportion of atherosclerosis patients carrying GG genotype of HSP60 rs2340690 was higher than those carrying AA genotype and AG genotype of HSP60 rs2340690 (p<0.05). The probability in atherosclerosis patients carrying rs788016 A was higher than those carrying rs2340690 G (p<0.05). When atherosclerosis patients carried both genotype G of HSP60 rs2340690 and genotype A of HSP60 rs788016, the odds ratio (OR) was 1.721 (p=0.049). Conclusions: The HSP60 gene polymorphisms are certainly correlated with the pathological grade and incidence rate of atherosclerosis.
MeSH term(s)	Asian Continental Ancestry Group/genetics ; Atherosclerosis/diagnosis ; Atherosclerosis/genetics ; Chaperonin 60/genetics ; Female ; Genotype ; Humans ; Male ; Middle Aged ; Mitochondrial Proteins/genetics ; Odds Ratio ; Polymorphism, Single Nucleotide/genetics
Chemical Substances	Chaperonin 60 ; HSPD1 protein, human ; Mitochondrial Proteins
Language	English
Publishing date	2020-03-20
Publishing country	Italy
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605550-3
ISSN	2284-0729 ; 1128-3602 ; 0392-291X
ISSN (online)	2284-0729
ISSN	1128-3602 ; 0392-291X
DOI	10.26355/eurrev_202003_20536
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1887: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Oligoasthenoteratospermia and sperm tail bending in PPP4C-deficient mice.

Han, F / Dong, M Z / Lei, W L / Xu, Z L / Gao, F / Schatten, H / Wang, Z B / Sun, X F / Sun, Q Y

Molecular human reproduction

2021 Volume 27, Issue 1

Abstract: Protein phosphatase 4 (PPP4) is a protein phosphatase that, although highly expressed in the testis, currently has an unclear physiological role in this tissue. Here, we show that deletion of PPP4 catalytic subunit gene Ppp4c in the mouse causes male- ... ...

Abstract	Protein phosphatase 4 (PPP4) is a protein phosphatase that, although highly expressed in the testis, currently has an unclear physiological role in this tissue. Here, we show that deletion of PPP4 catalytic subunit gene Ppp4c in the mouse causes male-specific infertility. Loss of PPP4C, when assessed by light microscopy, did not obviously affect many aspects of the morphology of spermatogenesis, including acrosome formation, nuclear condensation and elongation, mitochondrial sheaths arrangement and '9 + 2' flagellar structure assembly. However, the PPP4C mutant had sperm tail bending defects (head-bent-back), low sperm count, poor sperm motility and had cytoplasmic remnants attached to the middle piece of the tail. The cytoplasmic remnants were further investigated by transmission electron microscopy to reveal that a defect in cytoplasm removal appeared to play a significant role in the observed spermiogenesis failure and resulting male infertility. A lack of PPP4 during spermatogenesis causes defects that are reminiscent of oligoasthenoteratospermia (OAT), which is a common cause of male infertility in humans. Like the lack of functional PPP4 in the mouse model, OAT is characterized by abnormal sperm morphology, low sperm count and poor sperm motility. Although the causes of OAT are probably heterogeneous, including mutation of various genes and environmentally induced defects, the detailed molecular mechanism(s) has remained unclear. Our discovery that the PPP4C-deficient mouse model shares features with human OAT might offer a useful model for further studies of this currently poorly understood disorder.
MeSH term(s)	Animals ; Female ; Fertilization ; Fertilization in Vitro ; Infertility, Male/genetics ; Infertility, Male/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Inbred ICR ; Phosphoprotein Phosphatases/deficiency ; Phosphoprotein Phosphatases/metabolism ; Sperm Count ; Sperm Motility/genetics ; Sperm Tail/metabolism ; Sperm Tail/pathology ; Spermatogenesis/genetics
Chemical Substances	Phosphoprotein Phosphatases (EC 3.1.3.16) ; protein phosphatase 4 (EC 3.1.3.16)
Language	English
Publishing date	2021-02-03
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1324348-2
ISSN	1460-2407 ; 1360-9947
ISSN (online)	1460-2407
ISSN	1360-9947
DOI	10.1093/molehr/gaaa083
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 4482: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: [The predictive value of ureteral wall area for impacted ureteral stones].

Qi, W / Xi, J H / Yang, X L / Wu, W / Xu, Z L / Jing, J F / Ni, D W / Chen, Y / Wang, W / Zhang, Y B

Zhonghua yi xue za zhi

2021 Volume 101, Issue 44, Page(s) 3637–3642

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Female ; Humans ; Lithotripsy ; Male ; Retrospective Studies ; Treatment Outcome ; Ureter ; Ureteral Calculi/therapy ; Ureteroscopy
Language	Chinese
Publishing date	2021-11-25
Publishing country	China
Document type	Journal Article
ZDB-ID	132513-9
ISSN	0376-2491
ISSN	0376-2491
DOI	10.3760/cma.j.cn112137-20210325-00742
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 2011: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Effects of MiR-21 on the proliferation and migration of vascular smooth muscle cells in rats with atherosclerosis via the Akt/ERK signaling pathway.

Sun, P / Tang, L-N / Li, G-Z / Xu, Z-L / Xu, Q-H / Wang, M / Li, L

European review for medical and pharmacological sciences

2019 Volume 23, Issue 5, Page(s) 2216–2222

Abstract: Objective: To explore the effects and mechanism of action of micro ribonucleic acid (miR)-21 on the proliferation and migration of vascular smooth muscle (VSM) in atherosclerosis (AS).: Materials and methods: The rats were fed with a high-fat diet, ... ...

Abstract	Objective: To explore the effects and mechanism of action of micro ribonucleic acid (miR)-21 on the proliferation and migration of vascular smooth muscle (VSM) in atherosclerosis (AS). Materials and methods: The rats were fed with a high-fat diet, and the oil red staining was adopted to compare AS between Sprague Dawley (SD) rats and miR-21 knockdown rats. At the in-vitro level, primary rat VSM cells (VSMCs) were selected and divided into miR-NC blank control group [miR-normal control (NC) group] and miR-21 overexpression group (miR-21 group) for relevant experimental detection. Wound healing assay and transwell assay were used to detect the effects of miR-21 on the proliferation and migration of VSMCs. Westernn blotting was applied to examine the changes in the levels of Cyclin D, a cell cycle-related protein, and the key factors of the Akt/ERK signaling pathway, such as phosphorylated-Akt (p-AKT), AKT, p-ERK1/2, and ERK1/2. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell activity assay kit was applied to determine the effects of miR-21 on the proliferation of VSMCs through regulating the Akt/ERK signaling pathway after the ERK signaling pathway inhibitor PD98059 and AKT inhibitor MK-2206 were given. Results: Compared with that in miR-NC group, the level of AS in miR-21 knockdown rats were decreased significantly (p < 0.05). In the cell-level experiment, the overexpression of miR-21 promoted abnormal proliferation of VSMCs and activated the Akt/ERK signaling pathway (p < 0.05). MTT assay results revealed that inhibiting the Akt/ERK pathway could reverse the effects of miR-21 promoting proliferation and migration. Conclusions: MiR-21 promotes the proliferation and migration of VSMCs by activating the Akt/ERK pathway and aggravates AS. Knocking down miR-21 or inhibiting the Akt/ERK pathway can suppress the activation of VSMCs.
MeSH term(s)	Animals ; Atherosclerosis/chemically induced ; Atherosclerosis/genetics ; Cell Movement/drug effects ; Cell Proliferation/drug effects ; Cells, Cultured ; Diet, High-Fat/adverse effects ; Disease Models, Animal ; Flavonoids/pharmacology ; Gene Expression Regulation ; Gene Knockdown Techniques ; Heterocyclic Compounds, 3-Ring/pharmacology ; MAP Kinase Signaling System/drug effects ; Male ; MicroRNAs/genetics ; Muscle, Smooth, Vascular/cytology ; Muscle, Smooth, Vascular/drug effects ; Muscle, Smooth, Vascular/metabolism ; Phosphatidylinositol 3-Kinases/metabolism ; Phosphorylation ; Proto-Oncogene Proteins c-akt/metabolism ; Rats ; Rats, Sprague-Dawley
Chemical Substances	Flavonoids ; Heterocyclic Compounds, 3-Ring ; MK 2206 ; MicroRNAs ; mirn21 microRNA, rat ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (SJE1IO5E3I)
Language	English
Publishing date	2019-03-20
Publishing country	Italy
Document type	Comparative Study ; Journal Article
ZDB-ID	605550-3
ISSN	2284-0729 ; 1128-3602 ; 0392-291X
ISSN (online)	2284-0729
ISSN	1128-3602 ; 0392-291X
DOI	10.26355/eurrev_201903_17269
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1887: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: MicroRNA-424-5p inhibits the development of non-small cell LCa by binding to ITGB1.

Xu, Z-L / Zhang, M / Chen, S-X / Qiu, M / Zhang, Q / Gao, L-P / Li, J-W Du X-Q

European review for medical and pharmacological sciences

2019 Volume 23, Issue 20, Page(s) 8921–8930

Abstract: Objective: The aim of this study was to explore the effect of microRNA-424-5p on the proliferation and apoptosis of non-small cell lung cancer (NSCLC) cells, and to investigate its influence on the expression of ITGB1 and potential regulatory mechanism.! ...

Abstract	Objective: The aim of this study was to explore the effect of microRNA-424-5p on the proliferation and apoptosis of non-small cell lung cancer (NSCLC) cells, and to investigate its influence on the expression of ITGB1 and potential regulatory mechanism. Patients and methods: Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to detect the level of microRNA-424-5p in 44 paired NSCLC tissues and adjacent tissues. The relation between microRNA-424-5p expression and NSCLC clinical indicators was analyzed. Subsequently, microRNA-424-5p mimics and inhibitors were transfected into NSCLC cells to construct microRNA-424-5p overexpression or knockdown models, respectively. QRT-PCR was used to further verify the transfection efficiency. A series of experiments, including cell counting kit-8 (CCK-8) assay, colony formation, 5-Ethynyl-2'-deoxyuridine (EdU), and flow cytometry were used to analyze the effect of microRNA-424-5p on the biological function of NSCLC A549 and H358 cells. Finally, the potential association between microRNA-424-5p and its downstream gene ITGB1 was explored through luciferase reporter gene assay and cell recovery experiment. Results: QRT-PCR results showed that microRNA-424-5p level was significantly lower in NSCLC tissues than that of adjacent normal tissues. Compared with patients with high expression of microRNA-424-5p, the pathological stage of those with low expression of microRNA-424-5p was significantly higher. In vitro experiments showed that microRNA-424-5p overexpression remarkably decreased cell proliferation and increased cell apoptosis, which were further validated in microRNA-424-5p inhibitor group. Subsequently, ITGB1 expression was found significantly up-regulated in NSCLC cell lines and tissues. Meanwhile, ITGB1 expression was negatively correlated with microRNA-424-5p level. In addition, a recovery experiment indicated that overexpression of ITGB1 could counteract the effect of microRNA-424-5p mimics on the proliferation and apoptosis of NSCLC cells. All these findings revealed that microRNA-424-5p and ITGB1 affected the malignant progression of NSCLC. Conclusions: MicroRNA-424-5p was closely correlated with the pathological stage and poor prognosis of NSCLC, thereby inhibiting the occurrence and development of NSCLC.
MeSH term(s)	A549 Cells ; Carcinoma, Non-Small-Cell Lung/genetics ; Carcinoma, Non-Small-Cell Lung/pathology ; Cell Line, Tumor ; Disease Progression ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Integrin beta1/genetics ; Lung Neoplasms/genetics ; Lung Neoplasms/pathology ; MicroRNAs/genetics ; Neoplasm Staging ; Up-Regulation
Chemical Substances	Integrin beta1 ; Itgb1 protein, human ; MIRN424 microrna, human ; MicroRNAs
Language	English
Publishing date	2019-11-06
Publishing country	Italy
Document type	Journal Article
ZDB-ID	605550-3
ISSN	2284-0729 ; 1128-3602 ; 0392-291X
ISSN (online)	2284-0729
ISSN	1128-3602 ; 0392-291X
DOI	10.26355/eurrev_201910_19289
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1887: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito