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  1. Article: Autoinducer-2 of quorum sensing is involved in cell damage caused by avian pathogenic Escherichia coli

    Cui, Zhen-Qiang / Ben-Dong Fu / Dao-Xiu Xu / Hai-Qing Shen / Peng-Fei Yi / Xu-Bin Wei / Xun Guo / Yun-Xing Fu / Zong-Mei Wu

    Microbial pathogenesis. 2016 Oct., v. 99

    2016  

    Abstract: Avian pathogenic Escherichia coli (APEC) infections are responsible for great losses in the poultry industry. Quorum sensing (QS) acts as a global regulatory system that controls genes involved in bacterial pathogenesis, metabolism and protein ... ...

    Abstract Avian pathogenic Escherichia coli (APEC) infections are responsible for great losses in the poultry industry. Quorum sensing (QS) acts as a global regulatory system that controls genes involved in bacterial pathogenesis, metabolism and protein biosynthesis. However, whether QS of APEC is related to cell damage has not been elucidated. In the present study, we explored the correlation between the damage of chicken type II pneumocytes induced by APEC and the autoinducer-2 (AI-2) activity of APEC. The results showed that when chicken type II pneumocytes were co-cultured with 108 CFU/ml of APEC-O78 for 6 h, the release of LDH reached the highest level (192.5 ± 13.4 U/L) (P < 0.01), and the percentages of dead cells followed the same trend in trypan blue exclusion assay. In addition, the AI-2 activity of cell-free culture fluid (CF) reached the maximum value after 6 h co-culture with 108 CFU/ml of APEC-O78. At the same time, the mRNA expressions of eight virulence genes (papC, fimA, fimC, hlyE, ompA, luxS, pfs, and qseA) of 108 CFU/ml APEC-O78 were significantly increased compared with those of 107 CFU/ml, and the mRNA expressions of four virulence genes (hlyE, tsh, iss, and luxS) of 108 CFU/ml APEC-O78 were higher than those of 109 CFU/ml (p < 0.05) after incubation for 6 h. These results suggested that AI-2-mediated QS is involved in the cell damage induced by APEC-O78, indicating AI-2 may be one new potential target for preventing chicken colibacillosis.
    Keywords cell free system ; chickens ; coculture ; Escherichia coli ; genes ; messenger RNA ; pathogenesis ; pneumocytes ; poultry industry ; protein synthesis ; quorum sensing ; virulence
    Language English
    Dates of publication 2016-10
    Size p. 247-252.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 632772-2
    ISSN 1096-1208 ; 0882-4010
    ISSN (online) 1096-1208
    ISSN 0882-4010
    DOI 10.1016/j.micpath.2016.08.033
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2136: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article: Cinobufagin enhances the protective efficacy of formalin-inactivated Salmonella typhimurium vaccine through Th1 immune response

    Wu, Shuai-Cheng / Ben-Dong Fu / Dao-Xiu Xu / Hai-Qing Shen / Li-Yan Zhang / Peng-Fei Yi / Xu-Bin Wei / Xun Guo / Yun-Xing Fu / Zhen-Qiang Cui

    Microbial pathogenesis. 2016 Oct., v. 99

    2016  

    Abstract: Cinobufagin (CBG), one active ingredient isolated from Venenum Bufonis, has been demonstrated to have immunoregulatory effect. The aim of this study was to investigate whether CBG can enhance the protective efficacy of formalin-inactivated Salmonella ... ...

    Abstract Cinobufagin (CBG), one active ingredient isolated from Venenum Bufonis, has been demonstrated to have immunoregulatory effect. The aim of this study was to investigate whether CBG can enhance the protective efficacy of formalin-inactivated Salmonella typhimurium (FIST) in mice. ICR mice were immunized with FIST (106 CFU/mouse) alone or mixed with CBG (10, 20, and 40 μg) or alum (200 μg) on day 1 and day 15. Two weeks after the second immunization, serum and spleen were sampled for measuring FIST-specific antibody levels, cytokine levels, and splenocyte proliferation. The results showed that CBG enhanced FIST-specific IgG and IgG2a, the levels of interferon-gamma (IFNγ) and nitric oxide (NO), and the splenocyte proliferation response induced by concanavalin A, lipopolysaccharide, and FIST. In vivo protection studies showed that CBG significantly decreased the bacterial burdens in the spleen and prolonged the survival time of FIST-immunized mice challenged with live Salmonella typhimurium. In vivo IFNγ neutralization led to a significant reduction in FIST-specific IgG2a and IFNγ levels, and in the protective efficacy in CBG/FIST-immunized mice. In conclusion, CBG enhances the protective efficacy of formalin-inactivated Salmonella typhimurium vaccine by promoting the Th1 immune response.
    Keywords active ingredients ; alum ; antibodies ; blood serum ; concanavalin A ; cytokines ; immune response ; immunization ; immunoglobulin G ; interferon-gamma ; lipopolysaccharides ; mice ; neutralization ; nitric oxide ; Salmonella Typhimurium ; spleen ; splenocytes ; vaccines
    Language English
    Dates of publication 2016-10
    Size p. 264-270.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 632772-2
    ISSN 1096-1208 ; 0882-4010
    ISSN (online) 1096-1208
    ISSN 0882-4010
    DOI 10.1016/j.micpath.2016.08.036
    Database NAL-Catalogue (AGRICOLA)

    Full text online

    More links

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2136: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Effectiveness of Xiang-Qi-Tang against Avian Pathogenic Escherichia coli

    Chang-Liang He, , , , , , and / Ben-Dong Fu / Peng-Fei Yi / Xu-Bin Wei / Zhong-Qiong Yin / Cheng Lv / Wei Zhang / Xi-Mei Han / Xing-Li Cun

    Pakistan Veterinary Journal, Vol 34, Iss 1, Pp 127-

    2014  Volume 129

    Abstract: Xiang-Qi-Tang (XQT) is a Chinese medicine containing Rhizoma Cyperi (40 g), Andrographis paniculata (30 g) and Astragalus membranaceus (30 g). The purpose of this study is to investigate the therapeutic effects of XQT on Avian Pathogenic Escherichia coli ...

    Abstract Xiang-Qi-Tang (XQT) is a Chinese medicine containing Rhizoma Cyperi (40 g), Andrographis paniculata (30 g) and Astragalus membranaceus (30 g). The purpose of this study is to investigate the therapeutic effects of XQT on Avian Pathogenic Escherichia coli (APEC)-infected chickens. The chickens were pretreated with XQT 12 h before being inoculated with 108 colony forming unit (CFU) of APEC by subcutaneous injection, and then the mortality and the indexes of health status of chicken in each group were detected. The results showed that high dosage and middle dosage of XQT could significantly decrease the mortality of the chickens challenged with APEC. We further found that XQT improved the infected chickens’ health status through improving the water consumption, feed intake, bodyweight gain, routine blood parameters and decreasing the incidences of pericarditis and perihepatitis. The results of present study suggest that XQT can effectively treat chicken colibacillosis as a potential agent.
    Keywords Chicken colibacillosis ; Escherichia coli ; Herbal medicine ; Animal culture ; SF1-1100 ; Veterinary medicine ; SF600-1100
    Subject code 630
    Language English
    Publishing date 2014-01-01T00:00:00Z
    Publisher University of Agriculture, Faisalabad
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article: Inhibitory effects of α-cyperone on adherence and invasion of avian pathogenic Escherichia coli O78 to chicken type II pneumocytes

    Zhang, Li-Yan / Ben-Dong Fu / Cui Zhang / Fang Xia / Hai-Qing Shen / Peng-Fei Yi / Qian-Qian Qin / Shuai-Cheng Wu / Shuang Lv / Xi-Rou Hu / Xu-Bin Wei / Xun Guo / Zhou Song

    Veterinary immunology and immunopathology. 2014 May 15, v. 159, no. 1-2

    2014  

    Abstract: Avian pathogenic Escherichia coli (APEC) are extra-intestinal pathogenic E. coli, and usually cause avian septicemia through breaching the blood–gas barrier. Type II pneumocytes play an important role of maintaining the function of the blood–gas barrier. ...

    Abstract Avian pathogenic Escherichia coli (APEC) are extra-intestinal pathogenic E. coli, and usually cause avian septicemia through breaching the blood–gas barrier. Type II pneumocytes play an important role of maintaining the function of the blood–gas barrier. However, the mechanism of APEC injuring type II pneumocytes remains unclear. α-cyperone can inhibit lung cell injury induced by Staphylococcus aureus. In order to explore whether α-cyperone regulates the adherence and invasion of APEC-O78 to chicken type II pneumocytes, we successfully cultured chicken type II pneumocytes. The results showed that α-cyperone significantly decreased the adherence of APEC-O78 to chicken type II pneumocytes. In addition, α-cyperone inhibited actin cytoskeleton polymerization induced by APEC-O78 through down regulating the expression of Nck-2, Cdc42 and Rac1. These results provide new evidence for the prevention of colibacillosis in chicken.
    Keywords chickens ; Escherichia coli ; microfilaments ; pneumocytes ; polymerization ; septicemia ; Staphylococcus aureus
    Language English
    Dates of publication 2014-0515
    Size p. 50-57.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 754160-0
    ISSN 1873-2534 ; 0165-2427
    ISSN (online) 1873-2534
    ISSN 0165-2427
    DOI 10.1016/j.vetimm.2014.02.005
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2177: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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