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  1. Article ; Online: Use of interpretable machine learning to identify the factors influencing the nonlinear linkage between land use and river water quality in the Chesapeake Bay watershed

    Zhenyu Zhang / Jinliang Huang / Shuiwang Duan / Yaling Huang / Juntao Cai / Jing Bian

    Ecological Indicators, Vol 140, Iss , Pp 108977- (2022)

    2022  

    Abstract: Understanding the relationship between land use and water quality is essential for effective watershed management. However, it remains challenging to identify such a relationship owing to its nonlinearity. We developed an interpretable machine learning ... ...

    Abstract Understanding the relationship between land use and water quality is essential for effective watershed management. However, it remains challenging to identify such a relationship owing to its nonlinearity. We developed an interpretable machine learning method that integrated the random forest regression (RFR) model with the Shapley Additive exPlanations (SHAP) method to explore the relationship between water quality and land use in the Potomac River Basin (PRB), the second largest tributary entering Chesapeake Bay from 2006 to 2019. The water quality of the 26 sub-watersheds, classified into five types (natural, forested, agricultural, mixed, and urbanized), was investigated using statistical methods and scenario analysis. The results showed that the models employed were effective in predicting the water quality. The mean absolute error (MAE), root mean square error (RMSE), percent bias (PBIAS), R2 coefficient of determination (R2), and Kling-Gupta efficiency (KGE) were 0.011–0.159 mg/L, 0.019–0.219 mg/L, −0.14–0.64%, 0.79–0.99, and 0.69–0.98, respectively, during the training period, which were 0.010–0.201 mg/L, 0.017–0.292 mg/L, −1.87–0.41%, 0.82–0.99, and 0.80–0.97, respectively, during the testing period. The threshold effects of land use patterns were obvious for water quality indicators with high concentrations (i.e., TN and NH4+-N). In contrast, the water quality at low concentrations (i.e., TP and NO3–-N) may be more sensitive to wetland or barren land with changing climate. Agricultural activities and urbanization could be the dominant factors determining nutrient export to the PRB. Meanwhile, the typical ‘sink’ for the nutrient such as wetland may change into the ‘source’ for different nutrient. This study provides an in-depth understanding of how riverine nutrient export responds to the land use gradient in the Chesapeake Bay watershed.
    Keywords Land-use ; Water quality ; Nonlinearity ; Threshold effect ; Potomac River basin ; Ecology ; QH540-549.5
    Subject code 550
    Language English
    Publishing date 2022-07-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Landscape Characteristics in Mountain Parks across Different Urban Gradients and Their Relationship with Public Response

    Ziru Chen / Yuanping Sheng / Daosong Luo / Yaling Huang / Jingkai Huang / Zhipeng Zhu / Xiong Yao / Weicong Fu / Jiaying Dong / Yuxiang Lan

    Forests, Vol 14, Iss 12, p

    2023  Volume 2406

    Abstract: Numerous researchers have demonstrated the positive impacts of urban green spaces on human physiology and psychology. In mountainous urban regions, mountains have often been preserved as green spaces during urban sprawl, owing to the limited costs ... ...

    Abstract Numerous researchers have demonstrated the positive impacts of urban green spaces on human physiology and psychology. In mountainous urban regions, mountains have often been preserved as green spaces during urban sprawl, owing to the limited costs associated with development. While the landscape elements of these mountain parks exhibit differences depending on their locations, the nature and effects of such differences on the public’s physiological and psychological perceptions remain unclear. Therefore, we employed panoramic cameras and semantic segmentation (PSPNet-based training algorithm) to analyze the composition of landscape elements in mountain parks along an urban gradient (i.e., urban areas [UA], suburban areas [SA], and exurban areas [EA]). Concurrently, open-ended questionnaires and portable physiological monitors (ErgoLAB 3.0 Portable physiological monitoring equipment) were utilized to examine relationships between specific landscape elements and the public’s physiological and psychological responses. Our findings revealed that: (1) Urban park landscapes possessed high proportions of paved areas, humanistic vibe, vegetation hierarchy, and vegetation color richness, alongside lower scene clutter; suburban mountain park landscapes were characterized by heightened contemporary ambiance and wide viewshed area; and exurban mountain park landscapes exhibited high green view indices, expansive water surfaces, broad view area, and low scene clutter. (2) HRV and EMG differed significantly between mountain parks situated across the urban gradient. EMG also significantly varied across landscape types. All four psychological perception metrics showed significant distinctions across the three urban gradients and three green space categories. It further highlighted the importance of naturalness perception in urban mountain parks. (3) Viewshed area, average sight distance, architecture, enclosure, humanistic vibe, contemporary elements, vegetation color richness, trees and shrubs, distant hills, and scene clutter ...
    Keywords urban green spaces ; landscape elements ; semantic segmentation ; physiological and psychological perception ; Plant ecology ; QK900-989
    Subject code 710
    Language English
    Publishing date 2023-12-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Concurrent validity of the combined HRV/ACC sensor and physical activity diary when monitoring physical activity in university students during free-living days

    Haochong Liu / Qian Li / Yiting Li / Yubo Wang / Yaling Huang / Dapeng Bao / Haoyang Liu / Yixiong Cui

    Frontiers in Public Health, Vol

    2022  Volume 10

    Abstract: The purpose of this research was to determine if the scientific research device combined heart rate variability combined with an acceleration sensor (Firstbeat Bodyguard 2, BG2) was valid and reliable for time spent in different intensity zones in free- ... ...

    Abstract The purpose of this research was to determine if the scientific research device combined heart rate variability combined with an acceleration sensor (Firstbeat Bodyguard 2, BG2) was valid and reliable for time spent in different intensity zones in free-living. A total of 55 healthy participants performed 48-h physical activity (PA) monitoring with BG2, ActiGraph GT3X+ (GT3X+), and completed Bouchard Physical Activity Diary (Bouchard) every night. In the available studies, GT3X+ is considered the gold standard scientific research device for PA monitor. We compared BG2 and Bouchard with GT3X+ by difference, correlation, and agreement of PA and energy expenditure (EE) in free-living. The results showed that BG2 estimated PA more accurately than Bouchard, with a modest correlation (r > 0.49), strong agreement (τ > 0.29), and they had the lowest limits of agreement when estimating moderate to vigorous physical activity (MVPA). The EE estimated by Bouchard was the highest among the three methods, and the correlation and agreement between the three methods were high. Our findings showed that the BG2 is valid and reliable for estimating time spent in different intensity zones in free-living, especially in MVPA.
    Keywords physical activity ; energy expenditure ; validity ; intensity ; free-living ; Public aspects of medicine ; RA1-1270
    Subject code 796
    Language English
    Publishing date 2022-09-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Effect of Insulin Receptor on Juvenile Hormone Signal and Fecundity in Spodoptera litura (F.)

    Xue Pan / Yanfang Pei / Cuici Zhang / Yaling Huang / Ling Chen / Liqiong Wei / Chuanren Li / Xiaolin Dong / Xiang Chen

    Insects, Vol 13, Iss 8, p

    2022  Volume 701

    Abstract: Insulin signaling can regulate various physiological functions, such as energy metabolism and reproduction and so on, in many insects, including mosquito and locust. However, the molecular mechanism of this physiological process remains elusive. The ... ...

    Abstract Insulin signaling can regulate various physiological functions, such as energy metabolism and reproduction and so on, in many insects, including mosquito and locust. However, the molecular mechanism of this physiological process remains elusive. The tobacco cutworm, Spodoptera litura , is one of the most important pests of agricultural crops around the world. In this study, phosphoinositide 3-kinase ( SlPI3K ), protein kinase B ( SlAKT ), target of rapamycin ( SlTOR ), ribosomal protein S6 kinase ( SlS6K ) and transcription factor cAMP-response element binding protein ( SlCREB ) genes, except transcription factor forkhead box class O ( SlFoxO ), can be activated by bovine insulin injection. Then, we studied the influence of the insulin receptor gene ( SlInR ) on the reproduction of S. litura using RNA interference technology. qRT-PCR analysis revealed that SlInR was most abundant in the head. The SlPI3K , SlAKT , SlTOR , SlS6K and SlCREB genes were decreased, except SlFoxO, after the SlInR gene knockdown. Further studies revealed that the expression of vitellogenin mRNA and protein, Methoprene-tolerant gene ( SlMet ), could be down-regulated by the injection of dsRNA of SlInR significantly. Furthermore, a depletion in the insulin receptor by RNAi significantly decreased the content of juvenile hormone III (JH-III), total proteins and triacylgycerol. These changes indicated that a lack of SlInR could impair ovarian development and decrease fecundity in S. litura . Our studies contribute to a comprehensive insight into reproduction, regulated by insulin and the juvenile hormone signaling pathway through nutrition, and a provide theoretical basis for the reproduction process in pest insects.
    Keywords insulin pathway ; juvenile hormone ; reproduction ; vitellogenin ; nutrition ; Science ; Q
    Subject code 570
    Language English
    Publishing date 2022-08-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: A Coupled Modeling Approach for Water Management in a River–Reservoir System

    Zhenyu Zhang / Jinliang Huang / Min Zhou / Yaling Huang / Yimin Lu

    International Journal of Environmental Research and Public Health, Vol 16, Iss 16, p

    2019  Volume 2949

    Abstract: A coupled model is an effective tool to understand the nutrient fate associated with hydrodynamic and ecosystem processes and thereby developing a water resource management strategy. This paper presents a coupled modeling approach that consists of a ... ...

    Abstract A coupled model is an effective tool to understand the nutrient fate associated with hydrodynamic and ecosystem processes and thereby developing a water resource management strategy. This paper presents a coupled modeling approach that consists of a watershed model and a hydrodynamic model to evaluate the nutrient fate in a river−reservoir system. The results obtained from the model showed a good agreement with field observations. The results revealed that the Shuikou reservoir (Fuzhou, China)exhibited complicated hydrodynamic characteristics, which may induce the pattern of nutrient export. Reservoirs can greatly lower water quality as a result of decreasing water movement. Three scenarios were analyzed for water management. The NH 3 -N (Ammonia Nitrogen) decreased sharply in the outlet of Shuikou reservoir after NH 3 -N level in its tributary was reduced. After removing the farming cages, the water quality of the outlet of Shuikou reservoir was improved significantly. The DO (Dissolved Oxygen) had increased by 3%−10%, NH 3 -N had reduced by 5%−17%, and TP (Total Phosphorus) had reduced by 6%−21%. This study demonstrates that the proposed coupled modeling approach can effectively characterize waterway risks for water management in such a river−reservoir system.
    Keywords Hydrological Simulation Program Fortran (HSPF) ; Environmental Fluid Dynamics Code (EFDC) ; modeling ; river-reservoir continuum ; watershed management ; Medicine ; R
    Subject code 550
    Language English
    Publishing date 2019-08-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Spatial distributions of niche-constructing populations

    Xiaozhuo Han / Yaling Huang / Cang Hui

    Computational Ecology and Software, Vol 5, Iss 4, Pp 286-

    2015  Volume 298

    Abstract: Niche construction theory regards organisms not only as the object of natural selection but also an active subject that can change their own selective pressure through eco-evolutionary feedbacks. Through reviewing the existing works on the theoretical ... ...

    Abstract Niche construction theory regards organisms not only as the object of natural selection but also an active subject that can change their own selective pressure through eco-evolutionary feedbacks. Through reviewing the existing works on the theoretical models of niche construction, here we present the progress made on how niche construction influences genetic structure of spatially structured populations and the spatial-temporal dynamics of metapopulations, with special focuses on mathematical models and simulation methods. The majority of results confirmed that niche construction can significantly alter the evolutionary trajectories of structured populations. Organism-environmental interactions induced by niche construction can have profound influence on the dynamics, competition and diversity of metapopulations. It can affect fine-scale spatially distribution of species and spatial heterogeneity of the environment. We further propose a few research directions with potentials, such as applying adaptive dynamics or spatial game theory to explore the effect of niche construction on phenotypic evolution and diversification.
    Keywords organism-environmental feedback ; environmental heterogeneity ; individual-based model ; diversity ; Technology (General) ; T1-995 ; Science ; Q
    Subject code 690
    Language English
    Publishing date 2015-12-01T00:00:00Z
    Publisher International Academy of Ecology and Environmental Sciences
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article: Identification of AFB1-interacting proteins and interactions between RPSA and AFB1

    Zhuang, Zhenhong / Shihua Wang / Yaling Huang / Yanling Yang

    Journal of hazardous materials. 2016 Jan. 15, v. 301

    2016  

    Abstract: A method using immobilized affinity chromatography (IAC) was developed to screen for aflatoxin B1 (AFB1)-binding proteins. AFB1 and bovine serum albumin (BSA) coupled protein (BSA-AFB1) was prepared using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide ... ...

    Abstract A method using immobilized affinity chromatography (IAC) was developed to screen for aflatoxin B1 (AFB1)-binding proteins. AFB1 and bovine serum albumin (BSA) coupled protein (BSA-AFB1) was prepared using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride. The resulting coupled compound was immobilized onto PVDF transfer membranes, which were then incubated with total protein from mouse liver. AFB1-binding proteins were eluted, after non-specific washing, by specific elution, and the eluted proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Two candidate AFB1-binding proteins were identified by liquid chromatography–tandem mass spectrometry as the 40S ribosomal protein SA (RPSA) and a putative uncharacterized protein. RPSA and AFB1 interactions were further analyzed by ELISA in vitro and laser confocal immunofluorescence analysis in vivo. The results from ELISA and immunofluorescence showed that RPSA efficiently bound AFB1 in vitro and in vivo. This study’s conclusion laid the foundation for further exploration of the role of AFB1-binding proteins in AFB1 toxicology towards hepatocytes and the entry pathway of AFB1 into hepatocytes.
    Keywords affinity chromatography ; aflatoxin B1 ; bovine serum albumin ; enzyme-linked immunosorbent assay ; fluorescent antibody technique ; hepatocytes ; liver ; mice ; polyacrylamide gel electrophoresis ; protein content ; ribosomal proteins ; tandem mass spectrometry ; toxicology ; washing
    Language English
    Dates of publication 2016-0115
    Size p. 297-303.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1491302-1
    ISSN 1873-3336 ; 0304-3894
    ISSN (online) 1873-3336
    ISSN 0304-3894
    DOI 10.1016/j.jhazmat.2015.08.053
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: Coupled effects of natural and anthropogenic controls on seasonal and spatial variations of river water quality during baseflow in a coastal watershed of Southeast China.

    Jinliang Huang / Yaling Huang / Zhenyu Zhang

    PLoS ONE, Vol 9, Iss 3, p e

    2014  Volume 91528

    Abstract: Surface water samples of baseflow were collected from 20 headwater sub-watersheds which were classified into three types of watersheds (natural, urban and agricultural) in the flood, dry and transition seasons during three consecutive years (2010-2012) ... ...

    Abstract Surface water samples of baseflow were collected from 20 headwater sub-watersheds which were classified into three types of watersheds (natural, urban and agricultural) in the flood, dry and transition seasons during three consecutive years (2010-2012) within a coastal watershed of Southeast China. Integrating spatial statistics with multivariate statistical techniques, river water quality variations and their interactions with natural and anthropogenic controls were examined to identify the causal factors and underlying mechanisms governing spatiotemporal patterns of water quality. Anthropogenic input related to industrial effluents and domestic wastewater, agricultural activities associated with the precipitation-induced surface runoff, and natural weathering process were identified as the potential important factors to drive the seasonal variations in stream water quality for the transition, flood and dry seasons, respectively. All water quality indicators except SRP had the highest mean concentrations in the dry and transition seasons. Anthropogenic activities and watershed characteristics led to the spatial variations in stream water quality in three types of watersheds. Concentrations of NH(4)(+)-N, SRP, K(+), COD(Mn), and Cl- were generally highest in urban watersheds. NO3(-)N Concentration was generally highest in agricultural watersheds. Mg(2+) concentration in natural watersheds was significantly higher than that in agricultural watersheds. Spatial autocorrelations analysis showed similar levels of water pollution between the neighboring sub-watersheds exhibited in the dry and transition seasons while non-point source pollution contributed to the significant variations in water quality between neighboring sub-watersheds. Spatial regression analysis showed anthropogenic controls played critical roles in variations of water quality in the JRW. Management implications were further discussed for water resource management. This research demonstrates that the coupled effects of natural and anthropogenic controls involved in watershed processes, contribute to the seasonal and spatial variation of headwater stream water quality in a coastal watershed with high spatial variability and intensive anthropogenic activities.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2014-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Study on the apoptosis mechanism induced by T-2 toxin.

    Zhenhong Zhuang / Daibin Yang / Yaling Huang / Shihua Wang

    PLoS ONE, Vol 8, Iss 12, p e

    2013  Volume 83105

    Abstract: T-2 toxin is known to induce apoptosis in mammalian cells. The mechanism of apoptosis induced by T-2 toxin has been proposed to be linked with oxidative stress and mitochondrial pathway. In the current study, the toxic effect of T-2 on Hela, Bel-7402, ... ...

    Abstract T-2 toxin is known to induce apoptosis in mammalian cells. The mechanism of apoptosis induced by T-2 toxin has been proposed to be linked with oxidative stress and mitochondrial pathway. In the current study, the toxic effect of T-2 on Hela, Bel-7402, and Chang liver cells was examined in dose-dependent and time-dependent manner by MTT assay. Caspase-3 was found to be up-regulated under T-2 toxin stress, which suggested that T-2 toxin induced cell apoptosis. Endogenous GSH and MDA levels in all three cell lines were found down- and up-regulated respectively, which indicated the link between toxic effect of T-2 toxin and intracellular oxidative stress. It was also found by MTT assay that NAC, which maintained the level of GSH in cells, could protect cells from death. Western-blot result showed that the level of both activated Caspase-8 and Caspase-9 increased when cells were treated by T-2 toxin. Caspase-9 was found to be activated earlier than Caspase-8. It was also found that p53 was up-regulated under T-2 toxin stress in the study. These results implied that the effect of T-2 toxin on cells was apoptosis rather than necrosis, and it was probably induced through mitochondrial pathway. To the best of our knowledge, the present study is the first to show that JunD is down-regulated in T-2 toxin induced apoptosis. By construction of an over-expression vector for the JunD gene, we observed that the survival ratio of JunD over-expressed cells obviously increased under T-2 toxin stress. These results suggested that the mechanism of T-2 induced cell death was closely connected with oxidative stress, and that JunD plays an important role in the defensive process against T-2 toxin stress.
    Keywords Medicine ; R ; Science ; Q
    Subject code 570
    Language English
    Publishing date 2013-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Subchronic toxicity of dietary sulfamethazine and nanoplastics in marine medaka (Oryzias melastigma)

    Yu Ting Zhang / Hongxing Chen / Shuiqing He / Feipeng Wang / Yawen Liu / Mengyun Chen / Guangshan Yao / Yaling Huang / Ruanni Chen / Lingtian Xie / Jingli Mu

    Ecotoxicology and Environmental Safety, Vol 226, Iss , Pp 112820- (2021)

    Insights from the gut microbiota and intestinal oxidative status

    2021  

    Abstract: Antibiotics and nanoplastics are two prevalent pollutants in oceans, posing a great threat to marine ecosystems. As antibiotics and nanoplastics are highly bioconcentrated in lower trophic levels, evaluating their impacts on marine organisms via dietary ... ...

    Abstract Antibiotics and nanoplastics are two prevalent pollutants in oceans, posing a great threat to marine ecosystems. As antibiotics and nanoplastics are highly bioconcentrated in lower trophic levels, evaluating their impacts on marine organisms via dietary exposure route is of great importance. In this study, the individual and joint effects of dietborne sulfamethazine (SMZ) and nanoplastic fragments (polystyrene, PS) in marine medaka (Oryzias melastigma) were investigated. After 30 days of dietary exposure, 4.62 mg/g SMZ decreased the Chao1 index (60.86% for females and 26.85% for males) and the Shannon index (68.95% for females and 65.05% for males) and significantly altered the structure of gut microbial communities in both sexes. The female fish exposed to 4.62 mg/g SMZ exhibited higher intestinal sod (43.5%), cat (38.5%) and gpx (39.6%) transcripts, indicating oxidative stress in the gut. PS alone at 3.45 mg/g slightly altered the composition of the gut microbiota. Interestingly, the mixture of SMZ and PS caused more modest effects on the gut microbiota and intestinal antioxidant physiology than the SMZ alone, suggesting that the presence of PS might alleviate the intestinal toxicity of SMZ in a scenario of dietary co-exposure. This study helps better understand the risk of antibiotics and nanoplastics to marine ecosystems.
    Keywords Sulfamethazine (SMZ) ; Nanoplastics ; Dietary exposure ; Oryzias melastigma ; Gut microbiota ; Environmental pollution ; TD172-193.5 ; Environmental sciences ; GE1-350
    Subject code 333
    Language English
    Publishing date 2021-12-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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