Artikel ; Online: Sequential release of interacting proteins and Ub-modifying enzymes by disulfide heterotypic ubiquitin reagents.
2024 Band 145, Seite(n) 107186
Abstract: Heterotypic ubiquitin (Ub) chains have emerged as fundamental components in a wide range of cellular processes. The integrative identification of Ub-interacting proteins (readers) and Ub-modifying enzymes (writers and erasers) that selectively recognize ... ...
| Abstract | Heterotypic ubiquitin (Ub) chains have emerged as fundamental components in a wide range of cellular processes. The integrative identification of Ub-interacting proteins (readers) and Ub-modifying enzymes (writers and erasers) that selectively recognize and regulate heterotypic ubiquitination may provide crucial insights into these processes. In this study, we employed the bifunctional molecule-assisted (CAET) strategy to develop a type of disulfide bond-activated heterotypic Ub reagents, which allowed to enrich heterotypic Ub-interacting proteins and modifying enzymes simultaneously. The sequential release of readers which are non-covalently bound and writers or erasers which are covalently conjugated by using urea and reductant, respectively, combined with label-free quantitative (LFQ) MS indicated that these heterotypic Ub reagents would facilitate future investigations into functional roles played by heterotypic Ub chains. |
|---|---|
| Mesh-Begriff(e) | Ubiquitin/metabolism ; Indicators and Reagents ; Ubiquitination ; Proteins/metabolism ; Ubiquitin-Protein Ligases/metabolism |
| Chemische Substanzen | Ubiquitin ; Indicators and Reagents ; Proteins ; Ubiquitin-Protein Ligases (EC 2.3.2.27) |
| Sprache | Englisch |
| Erscheinungsdatum | 2024-02-09 |
| Erscheinungsland | United States |
| Dokumenttyp | Journal Article |
| ZDB-ID | 120080-x |
| ISSN | 1090-2120 ; 0045-2068 |
| ISSN (online) | 1090-2120 |
| ISSN | 0045-2068 |
| DOI | 10.1016/j.bioorg.2024.107186 |
| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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