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  1. Article ; Online: Assessment of Common Factors Associated with Droplet Digital PCR (ddPCR) Quantification of

    Lawaju, Bisho Ram / Yan, Guiping

    International journal of molecular sciences

    2024  Volume 25, Issue 6

    Abstract: This research investigated the factors associated with the quantitative detection ... ...

    Abstract This research investigated the factors associated with the quantitative detection of
    MeSH term(s) Animals ; Soil ; Polymerase Chain Reaction/methods ; Nematoda/genetics ; DNA/genetics
    Chemical Substances Soil ; DNA (9007-49-2)
    Language English
    Publishing date 2024-03-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25063104
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Host Suitability of Cover Crops to the Root-Lesion Nematode

    Neupane, Kamal / Yan, Guiping

    Plant disease

    2023  Volume 107, Issue 7, Page(s) 2096–2103

    Abstract: Nonhost or poor host cover crops can provide an alternative method for nematode management. A total of 25 cover crop species/cultivars, along with three controls were evaluated in greenhouse experiments for their host suitability to the root-lesion ... ...

    Abstract Nonhost or poor host cover crops can provide an alternative method for nematode management. A total of 25 cover crop species/cultivars, along with three controls were evaluated in greenhouse experiments for their host suitability to the root-lesion nematode
    MeSH term(s) Animals ; Solanum tuberosum ; Crops, Agricultural ; Lolium ; Soil ; Tylenchoidea
    Chemical Substances Soil
    Language English
    Publishing date 2023-07-11
    Publishing country United States
    Document type Journal Article
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS-08-22-2001-RE
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 2043: Show issues

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  3. Article ; Online: Host Suitability of Cover Crops to the Root-Lesion Nematode Pratylenchus penetrans Associated with Potato

    Neupane, Kamal / Yan, Guiping

    Plant Disease. 2023 July 01, v. 107, no. 7 p.2096-2103

    2023  

    Abstract: Nonhost or poor host cover crops can provide an alternative method for nematode management. A total of 25 cover crop species/cultivars, along with three controls were evaluated in greenhouse experiments for their host suitability to the root-lesion ... ...

    Abstract Nonhost or poor host cover crops can provide an alternative method for nematode management. A total of 25 cover crop species/cultivars, along with three controls were evaluated in greenhouse experiments for their host suitability to the root-lesion nematode Pratylenchus penetrans. Trials were conducted in a completely randomized design using nematode-infested soil and terminated 3 months after planting. Nematodes were extracted from the roots and soil of each crop to determine their final population densities, reproductive factor (Rf = final population density/initial population density), and distributions in the soil and root habitats. Reproductive factor was used to categorize the host suitability of crops. Faba bean cv. Petite produced the greatest nematode population density in all trials, whereas only alfalfa cv. Bullseye constantly demonstrated the poor host ability to P. penetrans. Annual ryegrass, winter rye cv. ND Dylan, and white proso millet also showed poor hosts in most trials. Five cover crops consistently maintained the population throughout the experiments, with Rf values less than 2, and the remaining tested cover crops were suitable hosts for P. penetrans. The majority of the tested cover crops had less than or equal to 30% of the final population residing in the roots after three months of growth in all the trials. This research helps us gain the knowledge on cover crops and P. penetrans interaction and will be useful for potato growers to select better cover crops and avoid susceptible hosts to manage P. penetrans in infested fields to minimize potato yield losses.
    Keywords Lolium ; Panicum miliaceum subsp. miliaceum ; Pratylenchus penetrans ; alfalfa ; cover crops ; cultivars ; faba beans ; greenhouses ; host preferences ; population density ; potatoes ; root lesion nematodes ; soil ; winter rye ; disease management ; host suitability ; nematodes ; potato ; reproductive factor ; root-lesion nematode ; vegetables
    Language English
    Dates of publication 2023-0701
    Size p. 2096-2103.
    Publishing place The American Phytopathological Society
    Document type Article ; Online
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS-08-22-2001-RE
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 2043: Show issues

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  4. Article: Early Detection and Temporal Dynamics of

    Arora, Deepika / Yan, Guiping

    Phytopathology

    2022  Volume 112, Issue 8, Page(s) 1776–1782

    Abstract: The root-lesion nematode, ...

    Abstract The root-lesion nematode,
    MeSH term(s) Animals ; Plant Diseases/parasitology ; Real-Time Polymerase Chain Reaction ; Solanum tuberosum/genetics ; Staining and Labeling ; Tylenchoidea/genetics
    Language English
    Publishing date 2022-07-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 208889-7
    ISSN 1943-7684 ; 0031-949X
    ISSN (online) 1943-7684
    ISSN 0031-949X
    DOI 10.1094/PHYTO-10-21-0412-R
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 698: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  5. Article: Screening of Early Maturing Soybean Accessions for Resistance Against HG Type 2.5.7 of Soybean Cyst Nematode Heterodera glycines

    Acharya, Krishna / Yan, Guiping

    Plant health progress. 2022 Mar. 17, v. 23, no. 2

    2022  

    Abstract: Soybean cyst nematode (SCN; Heterodera glycines) is one of the devastating soybean pests worldwide, including in the United States. Resistant cultivars combined with crop rotation are the primary methods for managing this nematode. SCN is known to have ... ...

    Abstract Soybean cyst nematode (SCN; Heterodera glycines) is one of the devastating soybean pests worldwide, including in the United States. Resistant cultivars combined with crop rotation are the primary methods for managing this nematode. SCN is known to have genetically diverse populations and can develop new virulent forms over time due to the continuous planting of cultivars derived from same source of resistance. Thus, identifying novel SCN resistant sources is of paramount importance for soybean breeding for nematode resistance. In this study, we screened 149 early maturity soybean [Glycine max (L.)] accessions for resistance to SCN HG type 2.5.7, which is one of the prevalent virulent SCN populations in North Dakota. SCN white females were extracted from individual plants of each accession after 35 days of growth in greenhouse conditions. The females were counted to determine a female index (FI = [average number of females on a tested accession/average number of females in Barnes, a susceptible soybean check] × 100). The resistance response of each soybean accession was categorized as resistant, moderately resistant, moderately susceptible, and susceptible. Of the 149 soybean accessions tested, only 13 were resistant in both runs of the experiments. The majority of screened soybean accessions were susceptible or moderately susceptible to the SCN HG type 2.5.7. The resistant soybean accessions identified in this study have the potential to be used in breeding SCN-resistant cultivars after further elucidation of the resistance genes or loci.
    Keywords Glycine max ; Heterodera glycines ; crop rotation ; cultivars ; early development ; females ; greenhouse production ; plant health ; soybeans ; virulence ; North Dakota
    Language English
    Dates of publication 2022-0317
    Size p. 166-173.
    Publishing place The American Phytopathological Society
    Document type Article
    ISSN 1535-1025
    DOI 10.1094/PHP-07-21-0105-RS
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Early Detection and Temporal Dynamics of Pratylenchus scribneri Infection in Potato Roots Determined Using Quantitative PCR and Root Staining

    Arora, Deepika / Yan, Guiping

    Phytopathology. 2022 Aug., v. 112, no. 8

    2022  

    Abstract: The root-lesion nematode, Pratylenchus scribneri, is a migratory endo-parasitic nematode that impacts potato production on a large scale. Effective management of this nematode requires an understanding of its population dynamics alongside early detection. ...

    Abstract The root-lesion nematode, Pratylenchus scribneri, is a migratory endo-parasitic nematode that impacts potato production on a large scale. Effective management of this nematode requires an understanding of its population dynamics alongside early detection. Typically, the nematode population estimates are made from infested soil; however, considering the endo-migratory lifestyle of this nematode, it also is crucial to determine the nematode population residing inside the host roots. In this study, a SYBR green-based quantitative real-time PCR (qPCR) assay was developed for detection and quantification of P. scribneri in potato roots. The assay used a previously reported primer pair (ITS-2F/ITS-2R), and it proved to be specific and sensitive, detecting as low as 1/128th equivalents of a P. scribneri individual per 0.2 g of potato roots. The robustness of the assay was reflected in high correlation observed between the P. scribneri densities determined microscopically and the densities detected by qPCR in artificially inoculated (R² = 0.93) and naturally infected (R² = 0.73) root samples. A time-course experiment conducted in the greenhouse using qPCR detected P. scribneri in potato roots as early as 5 days after planting. The results correlated well with the microscopic observations (R² = 0.80) and were complemented further with root staining. Additionally, three P. scribneri reproduction peaks were observed during one 3-month growth cycle of potato. Overall, the assay developed in this study is specific to P. scribneri in DNA extracts of root tissue and allows early detection and understandings of reproduction timings of this important nematode of potato.
    Keywords DNA ; Pratylenchus scribneri ; greenhouses ; lifestyle ; migratory behavior ; oligodeoxyribonucleotides ; plant pathology ; population dynamics ; potatoes ; quantitative polymerase chain reaction ; root lesion nematodes ; roots ; soil ; temporal variation
    Language English
    Dates of publication 2022-08
    Size p. 1776-1782.
    Publishing place The American Phytopathological Society
    Document type Article
    ZDB-ID 208889-7
    ISSN 1943-7684 ; 0031-949X
    ISSN (online) 1943-7684
    ISSN 0031-949X
    DOI 10.1094/PHYTO-10-21-0412-R
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 71/717: Show issues
    Z 2505: Show issues

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  7. Article ; Online: Development of a Droplet Digital PCR Assay for Detection and Quantification of Stubby Root Nematode, Paratrichodorus allius, in Soil

    Lawaju, Bisho Ram / Yan, Guiping / Whitworth, Jonathan

    Plant Disease. 2023 Nov. 01, v. 107, no. 11 p.3344-3353

    2023  

    Abstract: The stubby root nematode Paratrichodorus allius is an important plant-parasitic nematode species within the Trichodoridae family. It can directly harm the plants by feeding on the roots or indirectly by transmitting Tobacco rattle virus. These nematodes ... ...

    Abstract The stubby root nematode Paratrichodorus allius is an important plant-parasitic nematode species within the Trichodoridae family. It can directly harm the plants by feeding on the roots or indirectly by transmitting Tobacco rattle virus. These nematodes are mostly diagnosed either by traditional microscopic methods or a polymerase chain reaction (PCR)-based method. Droplet digital PCR (ddPCR) is a novel PCR technique which is sensitive and precise in quantifying DNA templates of the test samples. In this study, we developed a ddPCR assay to detect and quantify P. allius in soil. The specificity and sensitivity of the assay was first determined using P. allius nematode DNA or DNA from sterilized soil artificially inoculated with P. allius, and the assay was used to quantify P. allius populations in field soils. The assay did not detect nematodes other than P. allius, thus showing high specificity. It was able to detect P. allius equivalent to a 0.01 and 0.02 portion of a single nematode in soil DNA and nematode DNA extracts, respectively. Highly linear relationships between DNA copy numbers from ddPCR and serial dilutions of known concentrations were observed with DNA from P. allius nematodes (R² = 0.9842) and from artificially infested soil (R² = 0.9464). The P. allius populations from field soils determined by ddPCR were highly correlated with traditional microscopic counts (R² = 0.7963). To our knowledge, this is the first report of applying ddPCR to detect and quantify stubby root nematode in soil. The results of this study support the potentiality of a ddPCR assay as a new research tool in diagnostics of plant-parasitic nematodes.
    Keywords DNA ; Paratrichodorus ; Tobacco rattle virus ; droplets ; plant parasitic nematodes ; polymerase chain reaction ; soil ; soil sterilization ; detection ; droplet digital PCR ; identification ; Paratrichodorus allius ; quantification ; stubby root nematode
    Language English
    Dates of publication 2023-1101
    Size p. 3344-3353.
    Publishing place The American Phytopathological Society
    Document type Article ; Online
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS-03-23-0439-SR
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 2043: Show issues

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  8. Article: Editorial: Plant-parasitic nematode and plant interaction under abiotic and biotic stresses.

    Chen, Jingsheng / Guo, Xiaoli / Yan, Guiping / Hu, Yanfeng

    Frontiers in plant science

    2023  Volume 14, Page(s) 1321382

    Language English
    Publishing date 2023-11-07
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2023.1321382
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Development of a Droplet Digital PCR Assay for Detection and Quantification of Stubby Root Nematode,

    Lawaju, Bisho Ram / Yan, Guiping / Whitworth, Jonathan

    Plant disease

    2023  Volume 107, Issue 11, Page(s) 3344–3353

    Abstract: The stubby root ... ...

    Abstract The stubby root nematode
    MeSH term(s) Animals ; Soil/parasitology ; Nematoda/genetics ; Polymerase Chain Reaction/methods ; Plants ; Tylenchida/genetics ; DNA
    Chemical Substances Soil ; DNA (9007-49-2)
    Language English
    Publishing date 2023-11-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS-03-23-0439-SR
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 2043: Show issues

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  10. Article ; Online: Development of Real-Time and Conventional PCR Assays for Identifying a Newly Named Species of Root-Lesion Nematode (

    Chowdhury, Intiaz Amin / Yan, Guiping

    International journal of molecular sciences

    2021  Volume 22, Issue 11

    Abstract: A rapid and accurate PCR-based method was developed in this study for detecting and identifying a new species of root-lesion nematode ( ...

    Abstract A rapid and accurate PCR-based method was developed in this study for detecting and identifying a new species of root-lesion nematode (
    MeSH term(s) Animals ; DNA Primers/chemical synthesis ; DNA Primers/metabolism ; DNA, Helminth/genetics ; Limit of Detection ; North Dakota ; Plant Diseases/parasitology ; Plant Roots/parasitology ; Real-Time Polymerase Chain Reaction/methods ; Soil/parasitology ; Glycine max/parasitology ; Species Specificity ; Tylenchoidea/classification ; Tylenchoidea/genetics ; Tylenchoidea/isolation & purification
    Chemical Substances DNA Primers ; DNA, Helminth ; Soil
    Language English
    Publishing date 2021-05-30
    Publishing country Switzerland
    Document type Journal Article ; Validation Study
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms22115872
    Database MEDical Literature Analysis and Retrieval System OnLINE

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