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Article ; Online: Optimized Unilateral Magnetic Resonance Sensor with Constant Gradient and Its Applications in Composite Insulators.

Guo, Pan / Yang, Chenjie / Wu, Jiamin / Xu, Zheng

2023 Volume 23, Issue 12

Abstract: In this study, an optimized unilateral magnetic resonance sensor with a three-magnet array is presented for assessing the aging of composite insulators in power grids. The sensor's optimization involved enhancing the static magnetic field strength and ... ...

Abstract	In this study, an optimized unilateral magnetic resonance sensor with a three-magnet array is presented for assessing the aging of composite insulators in power grids. The sensor's optimization involved enhancing the static magnetic field strength and the homogeneity of the RF field while maintaining a constant gradient in the direction of the vertical sensor surface and maximizing homogeneity in the horizontal direction. The center layer of the target area was positioned 4 mm from the coil's upper surface, resulting in a magnetic field strength of 139.74 mT at the center point of the area, with a gradient of 2.318 T/m and a corresponding hydrogen atomic nuclear magnetic resonance frequency of 5.95 MHz. The magnetic field uniformity over a 10 mm × 10 mm range on the plane was 0.75%. The sensor measured 120 mm × 130.5 mm × 76 mm and weighed 7.5 kg. Employing the optimized sensor, magnetic resonance assessment experiments were conducted on composite insulator samples utilizing the CPMG (Carr-Purcell-Meiboom-Gill) pulse sequence. The T
MeSH term(s)	Magnetic Resonance Spectroscopy/methods ; Magnetic Resonance Imaging/methods ; Magnetic Fields ; Hydrogen
Chemical Substances	Hydrogen (7YNJ3PO35Z)
Language	English
Publishing date	2023-06-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s23125476
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Article: Dental and skeletal maturation of Chinese male children with unilateral cleft lip and palate.

Guo, Ying / Yang, Huiquan / Pan, Jing / Ren, Qianhui / Yang, Chenjie / Ji, Fang

The Journal of clinical pediatric dentistry

2024 Volume 48, Issue 2, Page(s) 40–46

Abstract: The aim of this study was to determine whether the relationship between dental age (DA), cervical stage (CS) and chronological age (CA) in Chinese male children with unilateral cleft lip and palate (UCLP) is similar to that of children without clefts. ... ...

Abstract	The aim of this study was to determine whether the relationship between dental age (DA), cervical stage (CS) and chronological age (CA) in Chinese male children with unilateral cleft lip and palate (UCLP) is similar to that of children without clefts. Panoramic and cephalometric radiographs of 105 male UCLP patients, aged from 8 to 16 years, were collected and compared to 210 age-matched healthy control males. The Demirjian and cervical vertebral maturation (CVM) methods were used to visually examine the radiographs and Spearman's correlation analysis was used to identify differences between the two groups with regards to CS, DA and CA. There was a significant positive correlation between DA and CA in both groups and the mean CA-DA difference was significantly higher in children with UCLP when compared to controls (0.319
MeSH term(s)	Child ; Humans ; Male ; Cleft Lip/diagnostic imaging ; Cleft Palate/diagnostic imaging ; Tooth ; China
Language	English
Publishing date	2024-03-03
Publishing country	Singapore
Document type	Journal Article
ZDB-ID	1062408-9
ISSN	1053-4628 ; 0145-5508
ISSN	1053-4628 ; 0145-5508
DOI	10.22514/jocpd.2024.032
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Article ; Online: High-Throughput Metabolic Pattern Screening Strategy for Early Colorectal and Gastric Cancers Based on Covalent Organic Frameworks-Assisted Laser Desorption/Ionization Mass Spectrometry.

Yang, Chenjie / Yu, Hailong / Li, Weihong / Lin, Hairu / Wu, Hao / Deng, Chunhui

Analytical chemistry

2024 Volume 96, Issue 16, Page(s) 6264–6274

Abstract: Precise early diagnosis and staging are conducive to improving the prognosis of colorectal cancer (CRC) and gastric cancer (GC) patients. However, due to intrusive inspections and limited sensitivity, the prevailing diagnostic methods impede precisely ... ...

Abstract	Precise early diagnosis and staging are conducive to improving the prognosis of colorectal cancer (CRC) and gastric cancer (GC) patients. However, due to intrusive inspections and limited sensitivity, the prevailing diagnostic methods impede precisely large-scale screening. In this work, we reported a high-throughput serum metabolic patterns (SMP) screening strategy based on covalent organic frameworks-assisted laser desorption/ionization mass spectrometry (hf-COFsLDI-MS) for early diagnosis and staging of CRC and GC. Notably, 473 high-quality SMP were extracted without any tedious sample pretreatment and coupled with multiple machine learning algorithms; the area under the curve (AUC) value is 0.938 with 96.9% sensitivity for early CRC diagnosis, and the AUC value is 0.974 with 100% sensitivity for early GC diagnosis. Besides, the discrimination of CRC and GC is accomplished with an AUC value of 0.966 for the validation set. Also, the screened-out features were identified by MS/MS experiments, and 8 metabolites were identified as the biomarkers for CRC and GC. Finally, the corresponding disordered metabolic pathways were revealed, and the staging of CRC and GC was completed. This work provides an alternative high-throughput screening strategy for CRC and GC and highlights the potential of metabolic molecular diagnosis in clinical applications.
MeSH term(s)	Humans ; Colorectal Neoplasms/diagnosis ; Colorectal Neoplasms/metabolism ; Stomach Neoplasms/diagnosis ; Stomach Neoplasms/metabolism ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods ; High-Throughput Screening Assays ; Early Detection of Cancer/methods ; Metal-Organic Frameworks/chemistry ; Male ; Middle Aged ; Female ; Biomarkers, Tumor/blood
Chemical Substances	Metal-Organic Frameworks ; Biomarkers, Tumor
Language	English
Publishing date	2024-04-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.3c05527
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Article ; Online: New Method for Counting and Picking Out Single Circulating Tumor Cells from Microliter-Volume Samples for Tumor Progression Surveillance and Single-Cell Heterogeneity Analysis.

Yu, Hailong / Yang, Chenjie / Tai, Qunfei / Gao, Mingxia / Zhang, Xiangmin

Analytical chemistry

2023 Volume 95, Issue 12, Page(s) 5232–5239

Abstract: Circulating tumor cells (CTCs) are crucial in tumor progression and metastasis, but the knowledge of their roles grows slowly at single-cell levels. Characterizing the rarity and fragility of CTCs by nature, highly stable and efficient single-CTC ... ...

Abstract	Circulating tumor cells (CTCs) are crucial in tumor progression and metastasis, but the knowledge of their roles grows slowly at single-cell levels. Characterizing the rarity and fragility of CTCs by nature, highly stable and efficient single-CTC sampling methods are still lacking, which impedes the development of single-CTC analysis. Herein, an improved, capillary-based single-cell sampling (SiCS) method, the so-called bubble-glue single-cell sampling (bubble-glue SiCS), is introduced. Benefiting from the characteristic that the cells tend to adhere to air bubbles in the solution, single cells can be sampled with bubbles as low as 20 pL with a self-designed microbubble-volume-controlled system. Benefiting from the excellent maneuverability, single CTCs are sampled directly from 10 μL volume of real blood samples after fluorescent labeling. Meanwhile, over 90% of the CTCs obtained survived and well proliferated after the bubble-glue SiCS process, which showed considerable superiority for downstream single-CTC profiling. Furthermore, a highly metastatic breast cancer model of the 4T1 cell line in vivo was employed for the real blood sample analysis. Increases in CTC numbers were observed during the tumor progression process, and significant heterogeneities among individual CTCs were discovered. In all, we propose a novel avenue for target SiCS and provide an alternative technique route for CTC separation and analysis.
MeSH term(s)	Humans ; Female ; Neoplastic Cells, Circulating/pathology ; Breast Neoplasms/pathology ; Neoplasm Metastasis ; Single-Cell Analysis/methods ; Biomarkers, Tumor
Chemical Substances	Biomarkers, Tumor
Language	English
Publishing date	2023-03-13
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.2c04994
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Article ; Online: Technological development of multidimensional liquid chromatography-mass spectrometry in proteome research.

Yu, Hailong / Tai, Qunfei / Yang, Chenjie / Gao, Mingxia / Zhang, Xiangmin

Journal of chromatography. A

2023 Volume 1700, Page(s) 464048

Abstract: Liquid chromatography-mass spectrometry (LC-MS) is the method of choice for high-throughput proteomic research. Limited by the peak capacity, the separation performance of conventional single-dimensional LC hampers the development of proteomics. ... ...

Abstract	Liquid chromatography-mass spectrometry (LC-MS) is the method of choice for high-throughput proteomic research. Limited by the peak capacity, the separation performance of conventional single-dimensional LC hampers the development of proteomics. Combining different separation modes orthogonally, multidimensional liquid chromatography (MDLC) with high peak capacity was developed to address this challenge. MDLC has evolved rapidly since its establishment, and the progress of proteomics has been greatly facilitated by the advent of novel MDLC-MS-based methods. In this paper, we will review the advances of MDLC-MS-based methodologies and technologies in proteomics studies, from different perspectives including novel application scenarios and proteomic targets, automation, miniaturization, and the improvement of the classic methods in recent years. In addition, attempts regarding new MDLC-MS models are also mentioned together with the outlook of MDLC-MS-based proteomics methods.
MeSH term(s)	Proteome/analysis ; Proteomics/methods ; Chromatography, Liquid/methods ; Mass Spectrometry/methods ; Automation
Chemical Substances	Proteome
Language	English
Publishing date	2023-05-04
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1171488-8
ISSN	1873-3778 ; 0021-9673
ISSN (online)	1873-3778
ISSN	0021-9673
DOI	10.1016/j.chroma.2023.464048
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Article ; Online: Assessment of Skeletal Maturation in Male Children With Unilateral Cleft Lip and Palate.

Yang, Chenjie / Shi, Jiajun / Chen, Zhenqi / Yu, Quan

The Journal of craniofacial surgery

2022 Volume 33, Issue 6, Page(s) e588–e592

Abstract: Objectives: To assess the skeletal maturation in male children with unilateral cleft lip and palate (UCLP) in comparison to that of noncleft peers using the cervical vertebral maturation (CVM) method.: Methods: A sample of 149 male UCLP patients aged ...

Abstract	Objectives: To assess the skeletal maturation in male children with unilateral cleft lip and palate (UCLP) in comparison to that of noncleft peers using the cervical vertebral maturation (CVM) method. Methods: A sample of 149 male UCLP patients aged from 8- to 16-year-old and 447 age-matched orthodontic individuals without clefts was retrospectively compiled. Cervical vertebral maturation was assessed based on the cephalometric radiographs. Results: The proportion of children in CVMSI and CVMSII was higher in the UCLP group compared to that in the noncleft group, but there was no significant difference in the CVM stage of the cleft patients compared to their noncleft peers. In the 12- to 14-year-old group, children with UCLP showed significantly delayed skeletal maturity in comparison with their noncleft peers. No significant difference was found in the other 3 age groups. There was a statistically significant correlation between the skeletal age and chronological age in both the UCLP group and the noncleft group. There was no significant difference in the mean age at CVMII and CVMIII between the cleft patients and noncleft peers. Conclusions: Males with UCLP aged 12- to 14-year-old have a statistically significant increased risk of delayed skeletal maturity in comparison with their noncleft peers. The chronological age is not an accurate indicator to assess the degree of skeletal maturation.
MeSH term(s)	Adolescent ; Cephalometry/methods ; Child ; Cleft Lip/diagnostic imaging ; Cleft Lip/surgery ; Cleft Palate/diagnostic imaging ; Cleft Palate/surgery ; Humans ; Male ; Retrospective Studies
Language	English
Publishing date	2022-03-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	1159501-2
ISSN	1536-3732 ; 1049-2275
ISSN (online)	1536-3732
ISSN	1049-2275
DOI	10.1097/SCS.0000000000008646
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Article ; Online: New Method for Counting and Picking Out Single Circulating Tumor Cells from Microliter-Volume Samples for Tumor Progression Surveillance and Single-Cell Heterogeneity Analysis

Yu, Hailong / Yang, Chenjie / Tai, Qunfei / Gao, Mingxia / Zhang, Xiangmin

Analytical Chemistry. 2023 Mar. 13, v. 95, no. 12 p.5232-5239

2023

Abstract	Circulating tumor cells (CTCs) are crucial in tumor progression and metastasis, but the knowledge of their roles grows slowly at single-cell levels. Characterizing the rarity and fragility of CTCs by nature, highly stable and efficient single-CTC sampling methods are still lacking, which impedes the development of single-CTC analysis. Herein, an improved, capillary-based single-cell sampling (SiCS) method, the so-called bubble-glue single-cell sampling (bubble-glue SiCS), is introduced. Benefiting from the characteristic that the cells tend to adhere to air bubbles in the solution, single cells can be sampled with bubbles as low as 20 pL with a self-designed microbubble-volume-controlled system. Benefiting from the excellent maneuverability, single CTCs are sampled directly from 10 μL volume of real blood samples after fluorescent labeling. Meanwhile, over 90% of the CTCs obtained survived and well proliferated after the bubble-glue SiCS process, which showed considerable superiority for downstream single-CTC profiling. Furthermore, a highly metastatic breast cancer model of the 4T1 cell line in vivo was employed for the real blood sample analysis. Increases in CTC numbers were observed during the tumor progression process, and significant heterogeneities among individual CTCs were discovered. In all, we propose a novel avenue for target SiCS and provide an alternative technique route for CTC separation and analysis.
Keywords	analytical chemistry ; blood ; blood sampling ; breast neoplasms ; cell lines ; fluorescence ; maneuverability ; metastasis ; models ; monitoring ; neoplasm progression
Language	English
Dates of publication	2023-0313
Size	p. 5232-5239.
Publishing place	American Chemical Society
Document type	Article ; Online
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.2c04994
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Article ; Online: Hollow Crystallization COF Capsuled MOF Hybrids Depict Serum Metabolic Profiling for Precise Early Diagnosis and Risk Stratification of Acute Coronary Syndrome.

Yang, Chenjie / Pan, Yilong / Yu, Hailong / Hu, Xufang / Li, Xiaodong / Deng, Chunhui

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

2023 Volume 10, Issue 24, Page(s) e2302109

Abstract: Acute coronary syndrome (ACS), comprising unstable angina (UA) and acute myocardial infarction (AMI), is the leading cause of death worldwide. Currently, lacking effective strategies for classifying ACS hinders the prognosis improvement of ACS patients. ... ...

Abstract	Acute coronary syndrome (ACS), comprising unstable angina (UA) and acute myocardial infarction (AMI), is the leading cause of death worldwide. Currently, lacking effective strategies for classifying ACS hinders the prognosis improvement of ACS patients. Disclosing the nature of metabolic disorders holds the potential to reflect disease progress and high-throughput mass spectrometry-based metabolic analysis is a promising tool for large-scale screening. Herein, a hollow crystallization COF capsuled MOF hybrids (UiO-66@HCOF) assisted serum metabolic analysis is developed for the early diagnosis and risk stratification of ACS. UiO-66@HCOF exhibits unrivaled chemical and structural stability as well as endowing satisfying desorption/ionization efficiency in the detection of metabolites. Paired with machine learning algorithms, early diagnosis of ACS is achieved with the area under the curve (AUC) value of 0.945 for validation sets. Besides, a comprehensive ACS risk stratification method is established, and the AUC value for the discrimination of ACS from healthy controls, and AMI from UA are 0.890, and 0.928. Moreover, the AUC value of the subtyping of AMI is 0.964. Finally, the potential biomarkers exhibit high sensitivity and specificity. This study makes metabolic molecular diagnosis a reality and provided new insight into the progress of ACS.
MeSH term(s)	Humans ; Acute Coronary Syndrome/diagnosis ; Crystallization ; Myocardial Infarction/diagnosis ; Angina, Unstable/diagnosis ; Early Diagnosis ; Risk Assessment/methods
Chemical Substances	UiO-66
Language	English
Publishing date	2023-06-20
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2808093-2
ISSN	2198-3844 ; 2198-3844
ISSN (online)	2198-3844
ISSN	2198-3844
DOI	10.1002/advs.202302109
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Article ; Online: Comparative transcriptomic analysis of the l-4i silkworm (Lepidoptera: Bombyx mori) mutants and its wild-type strain P33 by RNA-Seq.

Yang, Chenjie / Kang, Lequn / Zhao, Qiaoling

Comparative biochemistry and physiology. Part D, Genomics & proteomics

2021 Volume 38, Page(s) 100800

Abstract: The silkworm (Bombyx mori) is a domesticated holometabolous insect, and more than 400 Mendelian mutations have been identified. Investigating the mechanism behind these silkworm mutants is essential for understanding the development of silkworms and ... ...

Abstract	The silkworm (Bombyx mori) is a domesticated holometabolous insect, and more than 400 Mendelian mutations have been identified. Investigating the mechanism behind these silkworm mutants is essential for understanding the development of silkworms and other lepidopterans, and lethal genes could be used for pest control. The lethal silkworm mutant in the fourth instar (l-4i) has been recently found; however, the underlying mechanism is not yet clear. Herein, we studied the l-4i mutant and its wild-type strain P33 using RNA sequencing (RNA-seq). Our results revealed that 2013 genes were significantly downregulated, and 20 biological processes, including spliceosomal snRNP assembly, protein folding and protein catabolic process, were significantly enriched in these downregulated genes. Moreover, 2405 genes were significantly upregulated in the l-4i mutant, and 20 biological processes, including purine nucleobase metabolic process, nucleoside metabolic process and de novo IMP biosynthetic process, were significantly enriched in these upregulated genes. The study suggests that the imbalance of multiple biological processes and pathways and abnormal protein generation from RNA alternative splicing may cause the death of the l-4i mutant.
MeSH term(s)	Animals ; Bombyx/genetics ; Bombyx/physiology ; Genes, Insect ; Insect Proteins/genetics ; Mutation ; RNA-Seq ; Transcriptome
Chemical Substances	Insect Proteins
Language	English
Publishing date	2021-02-06
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2212119-5
ISSN	1878-0407 ; 1744-117X
ISSN (online)	1878-0407
ISSN	1744-117X
DOI	10.1016/j.cbd.2021.100800
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Article: Comparative transcriptomic analysis of the l-4i silkworm (Lepidoptera: Bombyx mori) mutants and its wild-type strain P33 by RNA-Seq

Yang, Chenjie / Kang, Lequn / Zhao, Qiaoling

Comparative biochemistry and physiology. 2021 June, v. 38

2021

Abstract	The silkworm (Bombyx mori) is a domesticated holometabolous insect, and more than 400 Mendelian mutations have been identified. Investigating the mechanism behind these silkworm mutants is essential for understanding the development of silkworms and other lepidopterans, and lethal genes could be used for pest control. The lethal silkworm mutant in the fourth instar (l-4i) has been recently found; however, the underlying mechanism is not yet clear. Herein, we studied the l-4i mutant and its wild-type strain P33 using RNA sequencing (RNA-seq). Our results revealed that 2013 genes were significantly downregulated, and 20 biological processes, including spliceosomal snRNP assembly, protein folding and protein catabolic process, were significantly enriched in these downregulated genes. Moreover, 2405 genes were significantly upregulated in the l-4i mutant, and 20 biological processes, including purine nucleobase metabolic process, nucleoside metabolic process and de novo IMP biosynthetic process, were significantly enriched in these upregulated genes. The study suggests that the imbalance of multiple biological processes and pathways and abnormal protein generation from RNA alternative splicing may cause the death of the l-4i mutant.
Keywords	Bombyx mori ; RNA ; biosynthesis ; catabolism ; death ; instars ; mutants ; nucleobases ; nucleosides ; pest control ; sequence analysis ; silkworms ; spliceosomes ; transcriptomics
Language	English
Dates of publication	2021-06
Publishing place	Elsevier Inc.
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	2212119-5
ISSN	1878-0407 ; 1744-117X
ISSN (online)	1878-0407
ISSN	1744-117X
DOI	10.1016/j.cbd.2021.100800
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