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  1. Article ; Online: Development and Feasibility Testing of a Decision Aid for Acute Appendicitis.

    Rosen, Joshua E / Yang, Frank F / Liao, Joshua M / Flum, David R / Kohler, Jonathan E / Agrawal, Nidhi A / Davidson, Giana H

    The Journal of surgical research

    2023  Volume 289, Page(s) 82–89

    Abstract: Introduction: Patients with acute uncomplicated appendicitis will be increasingly asked to choose between surgery and antibiotic management. We developed a novel decision aid for patients in the emergency department (ED) with acute appendicitis who are ... ...

    Abstract Introduction: Patients with acute uncomplicated appendicitis will be increasingly asked to choose between surgery and antibiotic management. We developed a novel decision aid for patients in the emergency department (ED) with acute appendicitis who are facing this choice. We describe the development of the decision aid and an initial feasibility study of its implementation in a busy tertiary care ED.
    Materials and methods: We conducted a prepost survey analysis comparing patients before and after standardized implementation of the decision aid. Patients were surveyed about their experience making treatment decisions after discharge from the hospital. The primary outcome measure was the total score on the decisional conflict scale (; 0-100; lower scores better).
    Results: The study included 24 participants (12 in the predecision aid period; 12 in the post period). Only 33% of participants in each group knew antibiotics were a treatment option prior to arriving at the ED. Prior to implementing the use of decision aid, only 75% of patients reported being told antibiotics were a treatment option, while this increased to 100% after implementation of the decision aid. The mean total decisional conflict scalescores were similar in the pre and post periods (mean difference = 0.13, 95% CI: -13 - 13, P > 0.9).
    Conclusions: This novel appendicitis decision aid was effectively integrated into clinical practice and helped toinform patients about multiple treatment options. These data support further large-scale testing of the decision aid as part of standardized pathways for the management of patients with acute appendicitis.
    MeSH term(s) Humans ; Decision Support Techniques ; Appendicitis/diagnosis ; Appendicitis/surgery ; Appendicitis/drug therapy ; Feasibility Studies ; Patient Participation ; Acute Disease ; Anti-Bacterial Agents/therapeutic use
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2023-04-20
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 80170-7
    ISSN 1095-8673 ; 0022-4804
    ISSN (online) 1095-8673
    ISSN 0022-4804
    DOI 10.1016/j.jss.2023.03.028
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: The pathological role and prognostic impact of miR-181 in acute myeloid leukemia.

    Weng, Hengyou / Lal, Kumar / Yang, Frank F / Chen, Jianjun

    Cancer genetics

    2015  Volume 208, Issue 5, Page(s) 225–229

    Abstract: In addition to genetic abnormalities, such as chromosomal translocations and somatic mutations that have been widely acknowledged in the leukemogenesis of acute myeloid leukemia (AML), epigenetic modifications also play a vital role in this process. ... ...

    Abstract In addition to genetic abnormalities, such as chromosomal translocations and somatic mutations that have been widely acknowledged in the leukemogenesis of acute myeloid leukemia (AML), epigenetic modifications also play a vital role in this process. MicroRNA (miRNA) regulation is emerging as a new layer of epigenetic regulation besides DNA methylation and histone modifications. Among the miRNAs first identified to be specifically expressed in hematopoietic cells, the miR-181 family has been implicated in regulating the differentiation of B cells, T cells, and natural killer cells during normal hematopoiesis, and has been linked tightly to the pathogenesis and prognosis of AML. Accumulating evidence indicates that miR-181 acts as a tumor suppressor in the pathogenesis of AML and exhibits a significant impact on the survival of patients with AML. Herein, we review the role of miR-181 as a diagnostic marker and prognostic predictor in AML, and discuss the potential use of miR-181 as a therapeutic target for AML.
    MeSH term(s) Biomarkers, Tumor/genetics ; Biomarkers, Tumor/physiology ; Epigenesis, Genetic ; Genes, Tumor Suppressor ; Hematopoiesis/genetics ; Humans ; Leukemia, Myeloid, Acute/diagnosis ; Leukemia, Myeloid, Acute/genetics ; Leukemia, Myeloid, Acute/mortality ; MicroRNAs/genetics ; MicroRNAs/physiology ; Prognosis
    Chemical Substances Biomarkers, Tumor ; MIrn181 microRNA, human ; MicroRNAs
    Language English
    Publishing date 2015-05
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2599227-2
    ISSN 2210-7762
    ISSN 2210-7762
    DOI 10.1016/j.cancergen.2014.12.006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Evaluation of an auditory assessment protocol for Chinese infants with nonsyndromic cleft lip and/or palate.

    Yang, Frank F / McPherson, Bradley / Shu, Huang

    The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association

    2012  Volume 49, Issue 5, Page(s) 566–573

    Abstract: Objective: To objectively investigate the auditory status of mainland Chinese infants with nonsyndromic cleft lip and/or palate and to evaluate an auditory assessment protocol for this group.: Design: Prospective cohort case review.: Patients or ... ...

    Abstract Objective: To objectively investigate the auditory status of mainland Chinese infants with nonsyndromic cleft lip and/or palate and to evaluate an auditory assessment protocol for this group.
    Design: Prospective cohort case review.
    Patients or other participants: A total of 42 Chinese infants with nonsyndromic cleft lip and/or palate aged 6 to 24 months.
    Intervention: The hearing profiles of participants were acquired using otoscopy, tympanometry, transient-evoked otoacoustic emissions, and auditory brainstem response estimated hearing threshold. The efficiency of the current audiological test battery was analyzed also.
    Results: Sixty-four (64.2) percent of infants with nonsyndromic cleft lip and/or palate (55.9% of ears) were suspected to have middle ear disorder, and 85.7% of all subjects (83.3% of ears) were found to have hearing loss. Of those with confirmed loss, the overall mean auditory brainstem response estimated air-conduction hearing threshold was 53.5 ± 13.6 decibel normal hearing level [db nHL]. Using auditory brainstem response thresholds as a reference standard, the diagnostic agreement with the other assessment tools for normal/abnormal results was otoscopy, 69% (κ  =  .57); tympanometry, 66.7% (κ  =  .53); and transient-evoked otoacoustic emissions, 80% (κ  =  .64).
    Conclusion: The high prevalence of middle ear disease and hearing loss in mainland Chinese infants with nonsyndromic cleft lip and/or palate in this study was in contrast to previous studies of older age groups of Chinese children conducted in Hong Kong and Singapore. The audiological assessment protocol used in our study could provide useful information; although, modifications may be necessary to ensure a complete diagnostic overview for children with craniofacial clefts.
    MeSH term(s) Acoustic Impedance Tests ; China/epidemiology ; Cleft Lip/complications ; Cleft Palate/complications ; Clinical Protocols ; Evoked Potentials, Auditory, Brain Stem ; Female ; Hearing Loss/diagnosis ; Hearing Loss/epidemiology ; Hearing Loss/etiology ; Humans ; Infant ; Male ; Otoacoustic Emissions, Spontaneous ; Otoscopy ; Prevalence ; Prospective Studies
    Language English
    Publishing date 2012-09
    Publishing country United States
    Document type Evaluation Studies ; Journal Article
    ZDB-ID 1069409-2
    ISSN 1545-1569 ; 0009-8701 ; 1055-6656
    ISSN (online) 1545-1569
    ISSN 0009-8701 ; 1055-6656
    DOI 10.1597/09-140.1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Fracture healing in osteoporotic bone.

    Cheung, Wing Hoi / Miclau, Theodore / Chow, Simon Kwoon-Ho / Yang, Frank F / Alt, Volker

    Injury

    2016  Volume 47 Suppl 2, Page(s) S21–6

    Abstract: As the world population rises, osteoporotic fracture is an emerging global threat to the well-being of elderly patients. The process of fracture healing by intramembranous ossification or/and endochondral ossification involve many well-orchestrated ... ...

    Abstract As the world population rises, osteoporotic fracture is an emerging global threat to the well-being of elderly patients. The process of fracture healing by intramembranous ossification or/and endochondral ossification involve many well-orchestrated events including the signaling, recruitment and differentiation of mesenchymal stem cells (MSCs) during the early phase; formation of a hard callus and extracellular matrix, angiogenesis and revascularization during the mid-phase; and finally callus remodeling at the late phase of fracture healing. Through clinical and animal research, many of these factors are shown to be impaired in osteoporotic bone. Animal studies related to post-menopausal estrogen deficient osteoporosis (type I) have shown healing to be prolonged with decreased levels of MSCs and decreased levels of angiogenesis. Moreover, the expression of estrogen receptor (ER) was shown to be delayed in ovariectomy-induced osteoporotic fracture. This might be related to the observed difference in mechanical sensitivity between normal and osteoporotic bones, which requires further experiments to elucidate. In mice fracture models related to senile osteoporosis (type II), it was observed that chondrocyte and osteoblast differentiation were impaired; and that transplantation of juvenile bone marrow would result in enhanced callus formation. Other factors related to angiogenesis and vasculogenesis have also been noted to be impaired in aged models, affecting the degradation of cartilaginous matrixes and vascular invasion; the result is changes in matrix composition and growth factors concentrations that ultimately impairs healing during age-related osteoporosis. Most osteoporotic related fractures occur at metaphyseal sites clinically, and reports have indicated that differences exist between diaphyseal and metaphyseal fractures. An animal model that satisfies three main criteria (metaphyseal region, plate fixation, osteoporosis) is suggested for future research for more comprehensive understanding of the impairment in osteoporotic fractures. Therefore, a metaphyseal fracture or osteotomy that achieves complete discontinuity fixed with metal implants is suggested on ovariectomized aged rodent models.
    MeSH term(s) Animals ; Biomechanical Phenomena ; Bony Callus/pathology ; Disease Models, Animal ; Estrogens/pharmacology ; Fracture Healing/drug effects ; Fractures, Bone/pathology ; Humans ; Osteogenesis ; Osteoporotic Fractures/pathology ; Osteotomy ; Ovariectomy
    Chemical Substances Estrogens
    Language English
    Publishing date 2016-06
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 218778-4
    ISSN 1879-0267 ; 0020-1383
    ISSN (online) 1879-0267
    ISSN 0020-1383
    DOI 10.1016/S0020-1383(16)47004-X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Central auditory nervous system dysfunction in infants with non-syndromic cleft lip and/or palate.

    Yang, Frank F / McPherson, Bradley / Shu, Huang / Xiao, Yuhan

    International journal of pediatric otorhinolaryngology

    2012  Volume 76, Issue 1, Page(s) 82–89

    Abstract: Objective: Peripheral hearing loss has been commonly reported in children with non-syndromic cleft lip and/or palate (NSCLP) but few studies have provided information about central auditory nervous system (CANS) functioning for this group. The main ... ...

    Abstract Objective: Peripheral hearing loss has been commonly reported in children with non-syndromic cleft lip and/or palate (NSCLP) but few studies have provided information about central auditory nervous system (CANS) functioning for this group. The main objective of this study was to explore CANS functioning in infants with NSCLP through analysis of auditory evoked potentials (AEPs).
    Methods: AEPs including auditory brainstem response (ABR), middle latency response (MLR), and mismatch negativity (MMN) recordings were conducted in 34 infants of Chinese ethnicity with NSCLP and an equivalent number of normal controls.
    Results: There was no significant difference in ABR (all measurements, including wave I, III, V latencies, I-V inter-wave latency, and wave V amplitude), or MLR (recordable components, Na, Pa latencies, and Na-Pa amplitude) findings between the two groups. However, infants with NSCLP had a significantly smaller MMN response than their normal controls, using MMN strength as the measurement.
    Conclusions: Significant abnormal auditory evoked potential findings at the cortical level suggest that infants with NSCLP may be at risk of central auditory discrimination dysfunction. Further effort is needed to determine auditory processing abilities in infants with NSCLP.
    MeSH term(s) Age Distribution ; Auditory Diseases, Central/diagnosis ; Auditory Diseases, Central/epidemiology ; Auditory Diseases, Central/etiology ; China ; Cleft Lip/diagnosis ; Cleft Lip/epidemiology ; Cleft Palate/diagnosis ; Cleft Palate/epidemiology ; Cohort Studies ; Evoked Potentials, Auditory, Brain Stem/physiology ; Female ; Follow-Up Studies ; Hearing Loss, Sensorineural/diagnosis ; Hearing Loss, Sensorineural/epidemiology ; Humans ; Incidence ; Infant ; Male ; Risk Assessment ; Severity of Illness Index ; Sex Distribution
    Language English
    Publishing date 2012-01
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 754501-0
    ISSN 1872-8464 ; 0165-5876
    ISSN (online) 1872-8464
    ISSN 0165-5876
    DOI 10.1016/j.ijporl.2011.10.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: The APC tumor suppressor is required for epithelial cell polarization and three-dimensional morphogenesis.

    Lesko, Alyssa C / Goss, Kathleen H / Yang, Frank F / Schwertner, Adam / Hulur, Imge / Onel, Kenan / Prosperi, Jenifer R

    Biochimica et biophysica acta

    2015  Volume 1853, Issue 3, Page(s) 711–723

    Abstract: The Adenomatous Polyposis Coli (APC) tumor suppressor has been previously implicated in the control of apical-basal polarity; yet, the consequence of APC loss-of-function in epithelial polarization and morphogenesis has not been characterized. To test ... ...

    Abstract The Adenomatous Polyposis Coli (APC) tumor suppressor has been previously implicated in the control of apical-basal polarity; yet, the consequence of APC loss-of-function in epithelial polarization and morphogenesis has not been characterized. To test the hypothesis that APC is required for the establishment of normal epithelial polarity and morphogenesis programs, we generated APC-knockdown epithelial cell lines. APC depletion resulted in loss of polarity and multi-layering on permeable supports, and enlarged, filled spheroids with disrupted polarity in 3D culture. Importantly, these effects of APC knockdown were independent of Wnt/β-catenin signaling, but were rescued with either full-length or a carboxy (c)-terminal segment of APC. Moreover, we identified a gene expression signature associated with APC knockdown that points to several candidates known to regulate cell-cell and cell-matrix communication. Analysis of epithelial tissues from mice and humans carrying heterozygous APC mutations further supports the importance of APC as a regulator of epithelial behavior and tissue architecture. These data also suggest that the initiation of epithelial-derived tumors as a result of APC mutation or gene silencing may be driven by loss of polarity and dysmorphogenesis.
    MeSH term(s) Adenomatous Polyposis Coli Protein/genetics ; Adenomatous Polyposis Coli Protein/physiology ; Animals ; Cell Culture Techniques ; Cell Polarity/genetics ; Cells, Cultured ; Dogs ; Epithelial Cells/physiology ; Gene Knockdown Techniques ; Genes, Tumor Suppressor/physiology ; HEK293 Cells ; Humans ; Mice ; Morphogenesis/genetics ; Mutation/physiology
    Chemical Substances APC protein, human ; Adenomatous Polyposis Coli Protein
    Language English
    Publishing date 2015-03
    Publishing country Netherlands
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 60-7
    ISSN 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    ISSN (online) 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650
    ISSN 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    DOI 10.1016/j.bbamcr.2014.12.036
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Structural abnormalities of the central auditory pathway in infants with nonsyndromic cleft lip and/or palate.

    Yang, Frank F / McPherson, Bradley / Shu, Huang / Xie, Na / Xiang, Kui

    The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association

    2012  Volume 49, Issue 2, Page(s) 137–145

    Abstract: Objective: To investigate possible structural abnormalities of the central auditory pathway in infants with nonsyndromic cleft lip and/or palate (NSCL/P).: Participants: Twenty-seven Chinese infants with NSCL/P, aged from 6 to 24 months.: ... ...

    Abstract Objective: To investigate possible structural abnormalities of the central auditory pathway in infants with nonsyndromic cleft lip and/or palate (NSCL/P).
    Participants: Twenty-seven Chinese infants with NSCL/P, aged from 6 to 24 months.
    Intervention: Morphological magnetic resonance imaging (MRI) measurements of the central auditory nervous system (CANS) in infants with NSCL/P were analyzed and compared with those of age- and sex-matched normal controls.
    Results: No significant group differences were found in general brain measurements, including volumes of the brain stem and right hemisphere. However, infants with NSCL/P had statistically significantly smaller volumes of the left thalamus and left auditory cortex and notably decreased thickness of the left auditory cortex.
    Conclusion: Cortical abnormalities were more marked compared with other MRI measurements. Structural CANS abnormalities in infants with NSCL/P may be located mainly in the left cerebral hemisphere. The development and maturation of the auditory cortex in infants with NSCL/P may be abnormal when compared with those of normal children.
    MeSH term(s) Audiometry ; Auditory Pathways/abnormalities ; China ; Cleft Lip/physiopathology ; Cleft Palate/physiopathology ; Female ; Humans ; Infant ; Magnetic Resonance Imaging ; Male
    Language English
    Publishing date 2012-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1069409-2
    ISSN 1545-1569 ; 0009-8701 ; 1055-6656
    ISSN (online) 1545-1569
    ISSN 0009-8701 ; 1055-6656
    DOI 10.1597/11-014
    Database MEDical Literature Analysis and Retrieval System OnLINE

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