Article ; Online: Quantification of Trophoblast Syncytialization by Fluorescent Membrane Labeling.
Methods in molecular biology (Clifton, N.J.)
2023 Volume 2728, Page(s) 99–104
Abstract: Trophoblast fusion or syncytialization is a fundamental yet poorly understood process during placental development. Primary cultured cytotrophoblasts and human choriocarcinoma cell lines are commonly used to study trophoblast fusion mechanisms in vitro. ... ...
| Abstract | Trophoblast fusion or syncytialization is a fundamental yet poorly understood process during placental development. Primary cultured cytotrophoblasts and human choriocarcinoma cell lines are commonly used to study trophoblast fusion mechanisms in vitro. Quantification of trophoblast fusion index is a key for the in vitro studies. In this chapter, we describe a new method to quantify fusion index, which is based on fluorescent labeling of the plasma membrane using Di-8-ANEPPS, a membrane potential dye. This method directly works on live cells, thereby is simple, economic, and reliable. |
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| MeSH term(s) | Pregnancy ; Humans ; Female ; Trophoblasts ; Placenta ; Membranes ; Cell Membrane ; Cell Line ; Coloring Agents |
| Chemical Substances | Coloring Agents |
| Language | English |
| Publishing date | 2023-11-01 |
| Publishing country | United States |
| Document type | Journal Article |
| ISSN | 1940-6029 |
| ISSN (online) | 1940-6029 |
| DOI | 10.1007/978-1-0716-3495-0_8 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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