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Article ; Online: Predicting Rural Ecological Space Boundaries in the Urban Fringe Area Based on Bayesian Network

Yangyang Yuan / Yuchen Yang / Ruijun Wang / Yuning Cheng

Land, Vol 11, Iss 1886, p

A Case Study in Nanjing, China

2022 Volume 1886

Abstract: Urban fringe areas are locations that compete between urban development and ecological protection; their ecological spatial boundaries face the risk of erosion and degradation. Previous studies have so far focused on the core area inside the ecological ... ...

Abstract	Urban fringe areas are locations that compete between urban development and ecological protection; their ecological spatial boundaries face the risk of erosion and degradation. Previous studies have so far focused on the core area inside the ecological space. However, research on the ecological boundary zone has so far been insufficient. The delineation of ECR is based on large-scale administrative units, while it is less precise at the level of small-scale rural areas. This study selected Paifang village in Nanjing City as the study area and built a Bayesian network model to predict the ecological space boundary for 2030. The study also identified the driving factors and their mechanisms affecting the changes in the rural ecological space in an urban fringe area and put forward targeted suggestions for its protection. The results suggested that: (1) The ecological space of Paifang village will expand in 2030. Specifically, agricultural land has the greatest potential for restoration of ecological space, followed by shrubland and grassland, and water bodies and their surrounding areas are potentially shrinking ecological space. (2) Artificial construction activities will disturb the ecological space, with the change in agricultural land being the main factor affecting the change in the ecological space boundary. (3) The Ecological Conservation Redline has a significant effect on the protection of the rural ecological space. The results of this study can provide a reference for rural planning and the formulation of protection policies in urban fringe areas.
Keywords	urban fringe area ; boundary prediction ; Bayesian network ; ecological space ; Ecological Conservation Redline ; Agriculture ; S
Subject code	720 ; 910
Language	English
Publishing date	2022-10-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Nursing Analysis Based on Medical Imaging Technology before and after Coronary Angiography in Cardiovascular Medicine

Qin Li / Yangyang Yuan / Guangyu Song / Yonghua Liu

Applied Bionics and Biomechanics, Vol

2022 Volume 2022

Abstract: With the advancement of technology, medical imaging technology has been greatly improved. This article mainly studies the nursing before and after coronary angiography in cardiovascular medicine based on medical imaging technology. This paper proposes a ... ...

Abstract	With the advancement of technology, medical imaging technology has been greatly improved. This article mainly studies the nursing before and after coronary angiography in cardiovascular medicine based on medical imaging technology. This paper proposes a multimodal medical image fusion algorithm based on multiscale decomposition and convolution sparse representation. The algorithm first decomposes the preregistered source medical image by NSST, takes the subimages of different scales as training images, and optimizes the subdictionaries of different scales; then convolution and sparse the subimages on each scale encoding to obtain the sparse coefficients of different subimages; secondly, the combination of improved L1 norm and improved spatial frequency (novel sum-modified SF (NMSF)) is used for high-frequency subimage coefficients, and the fusion of low-frequency subimages improved the rule of combining the L1 norm and the regional energy; finally, the final fused image is obtained by inverse NSST of the fused low-frequency subband and high-frequency subband. Experimental analysis found that the bifurcation angle has nothing to do with the damage of the branch vessels after the main branch stent is placed. The bifurcation angle greater than 50° is an independent predictor of MACE after stent extrusion for bifurcation lesions. Experimental results show that the proposed method has good performance in contrast enhancement, detail extraction, and information retention, and it improves the quality of the fusion image.
Keywords	Biotechnology ; TP248.13-248.65 ; Biology (General) ; QH301-705.5
Subject code	600
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Exposure to nanoplastics induces mitochondrial impairment and cytomembrane destruction in Leydig cells

Zhangbei Sun / Yiqian Wen / Fan Zhang / Zhendong Fu / Yangyang Yuan / Haibin Kuang / Xiaodong Kuang / Jian Huang / Liping Zheng / Dalei Zhang

Ecotoxicology and Environmental Safety, Vol 255, Iss , Pp 114796- (2023)

2023

Abstract: Plastic particle pollution poses an emerging threat to ecological and human health. Laboratory animal studies have illustrated that nano-sized plastics can accumulate in the testis and cause testosterone deficiency and spermatogenic impairment. In this ... ...

Abstract	Plastic particle pollution poses an emerging threat to ecological and human health. Laboratory animal studies have illustrated that nano-sized plastics can accumulate in the testis and cause testosterone deficiency and spermatogenic impairment. In this study, TM3 mouse Leydig cells were in vitro exposed to polystyrene nanoparticles (PS-NPs, size 20 nm) at dosages of 50, 100 and 150 μg/mL to investigate their cytotoxicity. Our results demonstrated that PS-NPs can be internalized into TM3 Leydig cells and led to a concentration-dependent decline in cell viability. Furthermore, PS-NPs stimulation amplified ROS generation and initiated cellular oxidative stress and apoptosis. Moreover, PS-NPs treatment affected the mitochondrial DNA copy number and collapsed the mitochondrial membrane potential, accompanied by a disrupted energy metabolism. The cells exposed to PS-NPs also displayed a down-regulated expression of steroidogenesis-related genes StAR, P450scc and 17β-HSD, along with a decrease in testosterone secretion. In addition, treatment with PS-NPs destructed plasma membrane integrity, as presented by increase in lactate dehydrogenase release and depolarization of cell membrane potential. In summary, these data indicated that exposure to PS-NPs in vitro produced cytotoxic effect on Leydig cells by inducing oxidative injury, mitochondrial impairment, apoptosis, and cytomembrane destruction. Our results provide new insights into male reproductive toxicity caused by NPs.
Keywords	PS-NPs ; Reproductive toxicity ; Oxidative stress ; Apoptosis ; Mitochondrial dysfunction ; Environmental pollution ; TD172-193.5 ; Environmental sciences ; GE1-350
Subject code	610
Language	English
Publishing date	2023-04-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Physiological Ovarian Aging Is Associated with Altered Expression of Post-Translational Modifications in Mice

Minli Wei / Jia Li / Huili Yan / Tao Luo / Jiang Huang / Yangyang Yuan / Liaoliao Hu / Liping Zheng

International Journal of Molecular Sciences, Vol 23, Iss 2, p

2022 Volume 2

Abstract: Post-translational modifications (PTMs) have been confirmed to be involved in multiple female reproductive events, but their role in physiological ovarian aging is far from elucidated. In this study, mice aged 3, 12 or 17 months (3M, 12M, 17M) were ... ...

Abstract	Post-translational modifications (PTMs) have been confirmed to be involved in multiple female reproductive events, but their role in physiological ovarian aging is far from elucidated. In this study, mice aged 3, 12 or 17 months (3M, 12M, 17M) were selected as physiological ovarian aging models. The expression of female reproductive function-related genes, the global profiles of PTMs, and the level of histone modifications and related regulatory enzymes were examined during physiological ovarian aging in the mice by quantitative real-time PCR and western blot, respectively. The results showed that the global protein expression of Kbhb (lysineβ-hydroxybutyryllysine), Khib (lysine 2-hydroxyisobutyryllysine), Kglu (lysineglutaryllysine), Kmal (lysinemalonyllysine), Ksucc (lysinesuccinyllysine), Kcr (lysinecrotonyllysine), Kbu (lysinebutyryllysine), Kpr (lysinepropionyllysine), SUMO1 (SUMO1 modification), ub (ubiquitination), P-Typ (phosphorylation), and 3-nitro-Tyr (nitro-tyrosine) increased significantly as mice aged. Moreover, the modification level of Kme2 (lysinedi-methyllysine) and Kac (lysineacetyllysine) was the highest in the 3M mice and the lowest in 12M mice. In addition, only trimethylation of histone lysine was up-regulated progressively and significantly with increasing age ( p < 0.001), H4 ubiquitination was obviously higher in the 12M and 17M mice than 3M ( p < 0.001), whereas the modification of Kpr (lysinepropionylation) and O-GlcNA in 17M was significantly decreased compared with the level in 3M mice ( p < 0.05, p < 0.01). Furthermore, the expression levels of the TIP60, P300, PRDM9, KMT5B, and KMT5C genes encoding PTM regulators were up-regulated in 17M compared to 3M female mice ( p < 0.05). These findings indicate that altered related regulatory enzymes and PTMs are associated with physiological ovarian aging in mice, which is expected to provide useful insights for the delay of ovarian aging and the diagnosis and treatment of female infertility.
Keywords	physiological ovarian aging ; histone modifications ; female reproductive functions ; post-translational modifications ; mice ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	570
Language	English
Publishing date	2022-12-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Genome-Wide Identification and Characterization of Apple P 3A -Type ATPase Genes, with Implications for Alkaline Stress Responses

Baiquan Ma / Meng Gao / Lihua Zhang / Haiyan Zhao / Lingcheng Zhu / Jing Su / Cuiying Li / Mingjun Li / Fengwang Ma / Yangyang Yuan

Forests, Vol 11, Iss 3, p

2020 Volume 292

Abstract: The P 3A -type ATPases play crucial roles in various physiological processes via the generation of a transmembrane H + gradient (∆pH). However, the P 3A -type ATPase superfamily in apple remains relatively uncharacterized. In this study, 15 apple P 3A - ... ...

Abstract	The P 3A -type ATPases play crucial roles in various physiological processes via the generation of a transmembrane H + gradient (∆pH). However, the P 3A -type ATPase superfamily in apple remains relatively uncharacterized. In this study, 15 apple P 3A -type ATPase genes were identified based on the new GDDH13 draft genome sequence. The exon-intron organization of these genes, the physical and chemical properties, and conserved motifs of the encoded enzymes were investigated. Analyses of the chromosome localization and ω values of the apple P 3A -type ATPase genes revealed the duplicated genes were influenced by purifying selection pressure. Six clades and frequent old duplication events were detected. Moreover, the significance of differences in the evolutionary rates of the P 3A -type ATPase genes were revealed. An expression analysis indicated that all of the P 3A -type ATPase genes were specifically expressed in more than one tissue. The expression of one P 3A -type ATPase gene ( MD15G1108400 ) was significantly upregulated in response to alkaline stress. Furthermore, a subcellular localization assay indicated that MD15G1108400 is targeted to the plasma membrane. These results imply that MD15G1108400 may be involved in responses to alkaline stress. Our data provide insights into the molecular characteristics and evolutionary patterns of the apple P 3A -type ATPase gene family and provide a theoretical foundation for future in-depth functional characterizations of P 3A -type ATPase genes under alkaline conditions.
Keywords	apple ; p 3a -type atpase ; evolutionary pattern ; expression pattern ; subcellular localization ; alkaline stress ; Plant ecology ; QK900-989
Subject code	290
Language	English
Publishing date	2020-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Genome-Wide Identification, Molecular Evolution, and Expression Divergence of Aluminum-Activated Malate Transporters in Apples

Baiquan Ma / Yangyang Yuan / Meng Gao / Tonghui Qi / Mingjun Li / Fengwang Ma

International Journal of Molecular Sciences, Vol 19, Iss 9, p

2018 Volume 2807

Abstract: Aluminum-activated malate transporters (ALMTs) play an important role in aluminum tolerance, stomatal opening, and fruit acidity in plants. However, the evolutionary pattern of the ALMT gene family in apples remains relatively unknown. In this study, a ... ...

Abstract	Aluminum-activated malate transporters (ALMTs) play an important role in aluminum tolerance, stomatal opening, and fruit acidity in plants. However, the evolutionary pattern of the ALMT gene family in apples remains relatively unknown. In this study, a total of 25 MdALMT genes were identified from the apple reference genome of the “Golden Delicious” doubled-haploid tree (GDDH13). The physiological and biochemical properties, gene structure, and conserved motifs of MdALMT genes were examined. Chromosome location and gene-duplication analysis indicated that whole-genome duplication/segmental duplication played an important role in the expansion of the MdALMT gene family. The Ka/Ks ratio of duplicated MdALMT genes showed that members of this family have undergone strong purifying selection. Through exploration of the phylogenetic relationships, seven subgroups were classified, and higher old gene duplication frequency and significantly different evolutionary rates of the ALMT gene families were detected. In addition, the functional divergence of ALMT genes occurred during the evolutionary process of Rosaceae species. Furthermore, the functional divergence of MdALMT genes was confirmed by expression discrepancy and different subcellular localizations. This study provides the foundation to better understand the molecular evolution of MdALMT genes and further facilitate functional analysis to unravel their exact role in apples.
Keywords	apple ; ALMT ; gene duplication ; expression profile ; subcellular localization ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	572
Language	English
Publishing date	2018-09-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Genome-wide Identification, Classification, Molecular Evolution and Expression Analysis of Malate Dehydrogenases in Apple

Baiquan Ma / Yangyang Yuan / Meng Gao / Libo Xing / Cuiying Li / Mingjun Li / Fengwang Ma

International Journal of Molecular Sciences, Vol 19, Iss 11, p

2018 Volume 3312

Abstract: Malate dehydrogenase plays crucial roles in energy homeostasis, plant development and cold and salt tolerance, as it mediates the reversible conversion of malate to oxaloacetate. However, the evolutionary pattern of MDH genes in apple remains elusive. In ...

Abstract	Malate dehydrogenase plays crucial roles in energy homeostasis, plant development and cold and salt tolerance, as it mediates the reversible conversion of malate to oxaloacetate. However, the evolutionary pattern of MDH genes in apple remains elusive. In this study, a total of 20 MDH genes were identified from the “Golden Delicious„ apple draft genome. We revealed the physiological and biochemical properties, gene structure, and conserved motifs of MdMDH genes. Chromosomal localization and Ka / Ks ratio analysis of MdMDH genes revealed different selective pressures acted on duplicated MdMDH genes. Exploration of the phylogenetic relationships revealed six clades and similar frequencies between old and recent duplications, and significant differences in the evolutionary rates of the MDH gene family were observed. One MdMDH gene, MDP0000807458, which was highly expressed during apple fruit development and flower bud differentiation, was under positive selection. Thus, we speculated that MDP0000807458 is a likely candidate gene involved in regulation of flower bud differentiation and organic acid metabolism in apple fruits. This study provides a foundation for improved understanding of the molecular evolution of MdMDH genes and further facilitates the functional analysis of MDP0000807458 to unravel its exact role in flower bud differentiation and organic acid metabolism.
Keywords	apple ; malate dehydrogenase ; evolutionary pattern ; positive selection ; expression profile ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	572
Language	English
Publishing date	2018-10-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Hydrogen transfer versus olefins methylation: On the formation trend of propene in the methanol-to-hydrocarbons reaction over Beta zeolites

Zhang, Jie / Lanjian Xu / Yanfei Zhang / Zhihua Huang / Xiaomin Zhang / Xinzhi Zhang / Yangyang Yuan / Lei Xu

Journal of catalysis. 2018 Dec., v. 368

2018

Abstract: Beta zeolites with similar textural properties but variable Brønsted acid site (BAS) densities in the range of 25.5–203.8 μmol g−1 were fabricated and investigated as catalysts in the methanol-to-hydrocarbons reaction. The intrinsic influence of BAS ... ...

Abstract	Beta zeolites with similar textural properties but variable Brønsted acid site (BAS) densities in the range of 25.5–203.8 μmol g−1 were fabricated and investigated as catalysts in the methanol-to-hydrocarbons reaction. The intrinsic influence of BAS amount, BAS density and methanol conversion on the reaction mechanism was investigated. Interestingly, methanol conversion depends on the BAS amount regardless of the BAS density. The increase of methanol concentration could modulate the product distribution by promoting the methanol-induced hydrogen transfer reaction and enhancing the olefins methylation as well as inhibiting the cracking of higher olefins. As the BAS density decreases from 203.8 to 56.6 μmol g−1, hydrogen transfer reaction is gradually restrained while the olefin interconversion is enhanced, leading to gradual replacement of aromatics-based cycle by the olefins-based cycle and thus increasing the propene selectivity. However, as BAS density further decreases from 56.6 μmol g−1, the propene selectivity declines because the methylation route in the olefins-based cycle is much more favoured than the cracking route. After detailed investigation of the deactivation behaviours of involved Beta zeolites, it has been found that the accumulation of long-chain alkanes and large polycyclic aromatics is responsible for the catalyst deactivation. These results provide further insights into the effect of BAS density on the reaction mechanism and product selectivity.
Keywords	Bronsted acids ; alkanes ; aromatic compounds ; catalysts ; cracking ; hydrogen ; methanol ; methylation ; propylene ; reaction mechanisms ; texture ; zeolites
Language	English
Dates of publication	2018-12
Size	p. 248-260.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	1468993-5
ISSN	0021-9517
ISSN	0021-9517
DOI	10.1016/j.jcat.2018.10.015
Database	NAL-Catalogue (AGRICOLA)

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Article: Exposure to 2,4-dichlorophenoxyacetic acid induces oxidative stress and apoptosis in mouse testis

Zhang, Dalei / Bei Yang / Changshui Xu / Haibin Kuang / Jianhua Yang / Lei Wu / Weiying Zou / Wenwen Liu / Yaling Wu / Yangyang Yuan

Pesticide biochemistry and physiology. 2017 Sept., v. 141

2017

Abstract: The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) is used worldwide. It has been associated with a variety of toxicities in rodents. In this study, male mice were orally administered 2,4-D at 50, 100 or 200mg/kg/day to investigate testicular toxicity ... ...

Abstract	The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) is used worldwide. It has been associated with a variety of toxicities in rodents. In this study, male mice were orally administered 2,4-D at 50, 100 or 200mg/kg/day to investigate testicular toxicity and the possible mechanisms of action. Exposure to 2,4-D at high concentrations (100 and 200mg/kg/day) for 14 consecutive days caused spermatogenic disruption and seminiferous epithelial destruction. Furthermore, 2,4-D administration (100 and 200mg/kg/day) increased the formation of the lipid peroxidation product malondialdehyde and decreased activities of the antioxidant enzymes superoxide dismutase and catalase in the testis. Moreover, 2,4-D exposure up-regulated the expression of p53 and Bax protein and down-regulated the expression of Bcl-2 protein in the testis. These results demonstrate that oxidative stress and apoptosis may be involved in testicular toxicity induced by high concentrations of 2,4-D in mice.
Keywords	2,4-D ; apoptosis ; catalase ; enzyme activity ; epithelium ; lipid peroxidation ; males ; malondialdehyde ; mechanism of action ; mice ; oral administration ; oxidative stress ; superoxide dismutase ; testes ; toxicity
Language	English
Dates of publication	2017-09
Size	p. 18-22.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	184819-7
ISSN	1095-9939 ; 0048-3583 ; 0048-3575
ISSN (online)	1095-9939
ISSN	0048-3583 ; 0048-3575
DOI	10.1016/j.pestbp.2016.10.006
Database	NAL-Catalogue (AGRICOLA)

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