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  1. AU="Yanxin Fan"
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  1. Article ; Online: Analyzing mitochondrial respiration of human induced pluripotent stem cell-derived myeloid progenitors using Seahorse technology

    Yanxin Fan / Yoko Mizoguchi / Megumi Tatematsu / Monika I. Linder / Stephanie Frenz / Jongsu Choi / Christoph Klein

    STAR Protocols, Vol 4, Iss 1, Pp 102073- (2023)

    2023  

    Abstract: Summary: Mitochondrial metabolism is critical in hematopoietic stem cell maintenance and differentiation. Here, we present a step-by-step protocol to efficiently differentiate human induced pluripotent stem cells into myeloid progenitors by a robust ... ...

    Abstract Summary: Mitochondrial metabolism is critical in hematopoietic stem cell maintenance and differentiation. Here, we present a step-by-step protocol to efficiently differentiate human induced pluripotent stem cells into myeloid progenitors by a robust feeder- and serum-free system. Furthermore, we provide a protocol to subsequently assess mitochondrial function in iPSC-derived myeloid progenitors. We comprehensively describe a protocol to analyze and to quantify key parameters of mitochondrial respiration of iPSC-derived myeloid progenitors by the Seahorse XFe96 Analyzer. Additionally, our protocol includes extensive troubleshooting suggestions.For complete details on the use and execution of this protocol, please refer to Fan et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
    Keywords Cell Biology ; Immunology ; Metabolism ; Stem Cells ; Cell Differentiation ; Science (General) ; Q1-390
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Circ-ZDHHC5 Accelerates Esophageal Squamous Cell Carcinoma Progression in vitro via miR-217/ZEB1 Axis

    Qian Wang / Lili Yang / Yanxin Fan / Weiwei Tang / Handong Sun / Zhipeng Xu / Jian Zhou / Yanzhou Zhang / Bin Zhu / Xiufeng Cao

    Frontiers in Cell and Developmental Biology, Vol

    2020  Volume 8

    Abstract: Circular RNA (circRNA) exhibits a covalently closed circular conformation and is structurally stable. Nevertheless, the precise effects exerted by circRNA in esophageal squamous cell carcinoma (ESCC) remains uncertain. circRNA was ascertained by a human ... ...

    Abstract Circular RNA (circRNA) exhibits a covalently closed circular conformation and is structurally stable. Nevertheless, the precise effects exerted by circRNA in esophageal squamous cell carcinoma (ESCC) remains uncertain. circRNA was ascertained by a human circRNA array study and was confirmed by the quantification of reverse transcriptase polymerase reactions. A luciferase reporter, fluorescence in situ hybridization experiment was exploited to explore the interaction between circ-ZDHHC5 and miR-217. The function of circ-ZDHHC5 was determined by siRNA-mediated knockout of circ-ZDHHC5 in in vitro proliferation, migration, and invasion. circ-ZDHHC5, rather than linear ZDHHC5 mRNA, rose in the tissues of patients with ESCC, plasma, and ESCC cell lines in comparison with normal controls. Knockdown of circ-ZDHHC5 inhibited tumorigenesis in ESCC cells, and the co-transfection of si-circ-ZDHHC5 and miR-217 mimics further enhanced the above effect. Noticeably, the present study showed that circ-ZDHHC5 was an miR-217 sponge that modulated the expression of zinc finger E-box binding homeobox 1 (ZEB1), further facilitating ESCC tumorigenesis. As revealed by this study, circ-ZDHHC5 can act as a new potential circular biomarker for detecting ESCC. It provides a novel perceptivity for the treatment of ESCC suggesting that circ-ZDHHC5 could impact on ESCC progression by sponging miR-217 with ZEB1.
    Keywords ESCC ; circ-ZDHHC5 ; miR-217 ; ZEB1 ; sponge ; Biology (General) ; QH301-705.5
    Subject code 500
    Language English
    Publishing date 2020-12-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: HAX1-dependent control of mitochondrial proteostasis governs neutrophil granulocyte differentiation

    Yanxin Fan / Marta Murgia / Monika I. Linder / Yoko Mizoguchi / Cong Wang / Marcin Łyszkiewicz / Natalia Ziȩtara / Yanshan Liu / Stephanie Frenz / Gabriela Sciuccati / Armando Partida-Gaytan / Zahra Alizadeh / Nima Rezaei / Peter Rehling / Sven Dennerlein / Matthias Mann / Christoph Klein

    The Journal of Clinical Investigation, Vol 132, Iss

    2022  Volume 9

    Abstract: The relevance of molecular mechanisms governing mitochondrial proteostasis to the differentiation and function of hematopoietic and immune cells is largely elusive. Through dissection of the network of proteins related to HCLS1-associated protein X-1, we ...

    Abstract The relevance of molecular mechanisms governing mitochondrial proteostasis to the differentiation and function of hematopoietic and immune cells is largely elusive. Through dissection of the network of proteins related to HCLS1-associated protein X-1, we defined a potentially novel functional CLPB/HAX1/(PRKD2)/HSP27 axis with critical importance for the differentiation of neutrophil granulocytes and, thus, elucidated molecular and metabolic mechanisms underlying congenital neutropenia in patients with HAX1 deficiency as well as bi- and monoallelic mutations in CLPB. As shown by stable isotope labeling by amino acids in cell culture (SILAC) proteomics, CLPB and HAX1 control the balance of mitochondrial protein synthesis and persistence crucial for proper mitochondrial function. Impaired mitochondrial protein dynamics are associated with decreased abundance of the serine-threonine kinase PRKD2 and HSP27 phosphorylated on serines 78 and 82. Cellular defects in HAX1–/– cells can be functionally reconstituted by HSP27. Thus, mitochondrial proteostasis emerges as a critical molecular and metabolic mechanism governing the differentiation and function of neutrophil granulocytes.
    Keywords Cell biology ; Immunology ; Medicine ; R
    Subject code 570
    Language English
    Publishing date 2022-05-01T00:00:00Z
    Publisher American Society for Clinical Investigation
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Author Correction

    Marcin Łyszkiewicz / Natalia Ziętara / Laura Frey / Ulrich Pannicke / Marcel Stern / Yanshan Liu / Yanxin Fan / Jacek Puchałka / Sebastian Hollizeck / Ido Somekh / Meino Rohlfs / Tuğba Yilmaz / Ekrem Ünal / Musa Karakukcu / Türkan Patiroğlu / Christina Kellerer / Ebru Karasu / Karl-Walter Sykora / Atar Lev /
    Amos Simon / Raz Somech / Joachim Roesler / Manfred Hoenig / Oliver T. Keppler / Klaus Schwarz / Christoph Klein

    Nature Communications, Vol 11, Iss 1, Pp 1-

    Human FCHO1 deficiency reveals role for clathrin-mediated endocytosis in development and function of T cells

    2020  Volume 1

    Abstract: An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    Abstract An amendment to this paper has been published and can be accessed via a link at the top of the paper.
    Keywords Science ; Q
    Language English
    Publishing date 2020-04-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Author Correction

    Marcin Łyszkiewicz / Natalia Ziętara / Laura Frey / Ulrich Pannicke / Marcel Stern / Yanshan Liu / Yanxin Fan / Jacek Puchałka / Sebastian Hollizeck / Ido Somekh / Meino Rohlfs / Tuğba Yilmaz / Ekrem Ünal / Musa Karakukcu / Türkan Patiroğlu / Christina Kellerer / Ebru Karasu / Karl-Walter Sykora / Atar Lev /
    Amos Simon / Raz Somech / Joachim Roesler / Manfred Hoenig / Oliver T. Keppler / Klaus Schwarz / Christoph Klein

    Nature Communications, Vol 11, Iss 1, Pp 1-

    Human FCHO1 deficiency reveals role for clathrin-mediated endocytosis in development and function of T cells

    2020  Volume 1

    Abstract: An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    Abstract An amendment to this paper has been published and can be accessed via a link at the top of the paper.
    Keywords Science ; Q
    Language English
    Publishing date 2020-04-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Human FCHO1 deficiency reveals role for clathrin-mediated endocytosis in development and function of T cells

    Marcin Łyszkiewicz / Natalia Ziętara / Laura Frey / Ulrich Pannicke / Marcel Stern / Yanshan Liu / Yanxin Fan / Jacek Puchałka / Sebastian Hollizeck / Ido Somekh / Meino Rohlfs / Tuğba Yilmaz / Ekrem Ünal / Musa Karakukcu / Türkan Patiroğlu / Christina Kellerer / Ebru Karasu / Karl-Walter Sykora / Atar Lev /
    Amos Simon / Raz Somech / Joachim Roesler / Manfred Hoenig / Oliver T. Keppler / Klaus Schwarz / Christoph Klein

    Nature Communications, Vol 11, Iss 1, Pp 1-

    2020  Volume 15

    Abstract: FCH domain only 1 (FCHO1) is a key molecule involved in clathrin-mediated endocytosis (CME). Here, the authors report homozygous FCHO1 mutations in individuals with variable T and B cell lymphopenia, which are associated with loss-of-function of FCHO1 ... ...

    Abstract FCH domain only 1 (FCHO1) is a key molecule involved in clathrin-mediated endocytosis (CME). Here, the authors report homozygous FCHO1 mutations in individuals with variable T and B cell lymphopenia, which are associated with loss-of-function of FCHO1 and impaired formation of clathrin-coated pits in T cells.
    Keywords Science ; Q
    Language English
    Publishing date 2020-02-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

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