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Article ; Online: Direct Identification of Complex Glycans via a Highly Sensitive Engineered Nanopore.

Yao, Guangda / Tian, Yinping / Ke, Wenjun / Fang, Jie / Ma, Shengzhou / Li, Tiehai / Cheng, Xi / Xia, Bingqing / Wen, Liuqing / Gao, Zhaobing

Journal of the American Chemical Society

2024

Abstract: The crucial roles that glycans play in biological systems are determined by their structures. However, the analysis of glycan structures still has numerous bottlenecks due to their inherent complexities. The nanopore technology has emerged as a powerful ... ...

Abstract	The crucial roles that glycans play in biological systems are determined by their structures. However, the analysis of glycan structures still has numerous bottlenecks due to their inherent complexities. The nanopore technology has emerged as a powerful sensor for DNA sequencing and peptide detection. This has a significant impact on the development of a related research area. Currently, nanopores are beginning to be applied for the detection of simple glycans, but the analysis of complex glycans by this technology is still challenging. Here, we designed an engineered α-hemolysin nanopore M113R/T115A to achieve the sensing of complex glycans at micromolar concentrations and under label-free conditions. By extracting characteristic features to depict a three-dimensional (3D) scatter plot, glycans with different numbers of functional groups, various chain lengths ranging from disaccharide to decasaccharide, and distinct glycosidic linkages could be distinguished. Molecular dynamics (MD) simulations show different behaviors of glycans with β1,3- or β1,4-glycosidic bonds in nanopores. More importantly, the designed nanopore system permitted the discrimination of each glycan isomer with different lengths in a mixture with a separation ratio of over 0.9. This work represents a proof-of-concept demonstration that complex glycans can be analyzed using nanopore sequencing technology.
Language	English
Publishing date	2024-04-11
Publishing country	United States
Document type	Journal Article
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021/jacs.4c02081
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Article ; Online: The effect of Banxia-houpo decoction on CUMS-induced depression by promoting M2 microglia polarization via TrkA/Akt signalling.

Liu, Li / Zhang, Rong / Chen, Chang / Xia, Changbo / Yao, Guangda / He, Xiaogang / Xia, Baomei

Journal of cellular and molecular medicine

2023 Volume 27, Issue 21, Page(s) 3339–3353

Abstract: It has been reported that Banxia-houpo decoction (BXHPD) serves as the anti-depressant treatment for a mild and severe depressive disease with limited side effects. The present study was performed to evaluate the protective effect of BXHPD on chronic ... ...

Abstract	It has been reported that Banxia-houpo decoction (BXHPD) serves as the anti-depressant treatment for a mild and severe depressive disease with limited side effects. The present study was performed to evaluate the protective effect of BXHPD on chronic unpredicted mild stress (CUMS)-induced depression and explore its effect on TrkA/Akt-mediated microglia polarization. The CUMS procedure was carried out, and the mice were intragastrically treated with BXHPD once daily. The selective TrkA inhibitor GW441756 was applied to further investigate the role of TrkA in BXHPD-mediated microglia polarization. The behaviour test including open field test (OFT), sucrose preference test (SPT), novelty-suppressed feeding test (NSFT), tail suspension test (TST) and forced swim test (FST) was performed. The concentrations of pro-inflammatory cytokines IL-6, TNF-α, IL-1β, IL-12 and anti-inflammatory cytokines IL-4, IL-10 were determined using Enzyme-linked immunosorbent assay. The population of Iba1+ cells and the length of microglia processes were observed under the fluorescence microscope. The mRNA expressions of Arg1, Ym1 and Fizzl1 were measured by PCR. The protein expressions of TrkA, p-Tyr490-TrkA, p-Ser473-Akt, p-Ser473-Akt1, p-Ser474-Akt2, p-CREB and Jmjd3 were detected by western blot. Our results showed that BXHPD attenuated CUMS-induced depressive-like behaviour, promoted anti-inflammatory cytokines, inhibited pro-inflammatory cytokines, suppressed microglia activation, promoted M2 phenotype-specific indices and upregulated the expressions of TrkA, p-Tyr490-TrkA, p-Ser473-Akt, p-Ser473-Akt1, p-Ser474-Akt2, p-CREB and Jmjd3. The above beneficial effect of BXHPD can be blocked by TrkA inhibitor GW441756. This work demonstrated that BXHPD exerted an anti-depressant effect by promoting M2 phenotype microglia polarization via TrkA/Akt pathway.
MeSH term(s)	Mice ; Animals ; Depression/drug therapy ; Depression/etiology ; Depression/metabolism ; Proto-Oncogene Proteins c-akt/metabolism ; Antidepressive Agents/pharmacology ; Antidepressive Agents/therapeutic use ; Microglia/metabolism ; Behavior, Animal ; Cytokines/metabolism ; Anti-Inflammatory Agents/pharmacology ; Stress, Psychological/complications ; Stress, Psychological/drug therapy ; Stress, Psychological/metabolism ; Disease Models, Animal ; Hippocampus/metabolism
Chemical Substances	Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Antidepressive Agents ; Cytokines ; Anti-Inflammatory Agents
Language	English
Publishing date	2023-08-15
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2074559-X
ISSN	1582-4934 ; 1582-4934 ; 1582-1838
ISSN (online)	1582-4934
ISSN	1582-4934 ; 1582-1838
DOI	10.1111/jcmm.17906
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Article: The Protective Effect of Pilose Antler Peptide on CUMS-Induced Depression Through AMPK/Sirt1/NF-κB/NLRP3-Mediated Pyroptosis.

Hu, Yue / Zhao, Min / Zhao, Tong / Qi, Mingming / Yao, Guangda / Dong, Yu

Frontiers in pharmacology

2022 Volume 13, Page(s) 815413

Abstract: Background: ...

Abstract	Background:
Language	English
Publishing date	2022-03-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2022.815413
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Article ; Online: Astragalin attenuates depression-like behaviors and memory deficits and promotes M2 microglia polarization by regulating IL-4R/JAK1/STAT6 signaling pathway in a murine model of perimenopausal depression.

Yao, Guangda / Bai, Zijun / Niu, Jianguo / Zhang, Rui / Lu, Youyuan / Gao, Tiantian / Wang, Hanqing

Psychopharmacology

2022 Volume 239, Issue 8, Page(s) 2421–2443

Abstract: Rationale: Neuroinflammation can be alleviated via M2 microglia polarization, which could promote the recovery of perimenopausal depression. Astragalin (AST) possesses anti-neuroinflammatory activity. However, the effects of AST on perimenopausal ... ...

Abstract	Rationale: Neuroinflammation can be alleviated via M2 microglia polarization, which could promote the recovery of perimenopausal depression. Astragalin (AST) possesses anti-neuroinflammatory activity. However, the effects of AST on perimenopausal depression and the molecular mechanism in regulating microglia polarization remained unknown. Objectives: The purpose was to investigate the effects of AST on mice with simulated perimenopausal depression through regulating microglia polarization. It was aimed to clarify the molecular mechanism related to the interleukin-4 receptor (IL-4R)/janus kinase (JAK) 1/signal transducer and activator of transcription (STAT) 6 signaling pathway. Methods: The ovariectomy (OVX)/chronic unpredictable mild stress (CUMS)-induced murine model of perimenopausal depression was established and treated with AST. Then the depression-like behaviors and cognitive ability of mice were examined. After that, we detected the markers of microglia polarization and its regulatory signals. In addition, lipopolysaccharides (LPS)/adenosine triphosphate (ATP)-induced inflammatory BV2 model were used to verify the potential molecular mechanism. Results: AST alleviated perimenopausal depression-like behaviors and memory deficits. AST alleviated microglia activation and increased Ki67-positive cells in dentate gyrus (DG). The viability of BV2 decreased by LPS/ATP was raised by AST. Moreover, both in vivo and in vitro, AST switched microglia from M1 phenotype caused by OVX/CUMS or LPS/ATP to M2 phenotype. The IL-4R/JAK1/STAT6 signaling was restored, and the levels of inducible nitric oxide synthase (iNOS), nuclear NF-KappaB-p65 were reduced by AST. Importantly, AST showed prevention against the ubiquitination modification and degradation of STAT6. Conclusions: Our results revealed new insights into molecular mechanism associated with microglia polarization in the effect of AST on the mouse model of perimenopausal depression.
MeSH term(s)	Adenosine Triphosphate/metabolism ; Adenosine Triphosphate/pharmacology ; Animals ; Depression/drug therapy ; Disease Models, Animal ; Female ; Kaempferols ; Lipopolysaccharides/pharmacology ; Memory Disorders/metabolism ; Mice ; Microglia/metabolism ; Perimenopause ; Receptors, Interleukin-4/metabolism ; STAT6 Transcription Factor/metabolism ; STAT6 Transcription Factor/pharmacology ; Signal Transduction
Chemical Substances	Kaempferols ; Lipopolysaccharides ; Receptors, Interleukin-4 ; STAT6 Transcription Factor ; Stat6 protein, mouse ; Adenosine Triphosphate (8L70Q75FXE) ; astragalin (APM8UQ3Z9O)
Language	English
Publishing date	2022-04-12
Publishing country	Germany
Document type	Journal Article
ZDB-ID	130601-7
ISSN	1432-2072 ; 0033-3158
ISSN (online)	1432-2072
ISSN	0033-3158
DOI	10.1007/s00213-022-06133-5
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Article ; Online: Mapping the Acetylamino and Carboxyl Groups on Glycans by Engineered α-Hemolysin Nanopores.

Xia, Bingqing / Fang, Jie / Ma, Shengzhou / Ma, Mengyao / Yao, Guangda / Li, Tiehai / Cheng, Xi / Wen, Liuqing / Gao, Zhaobing

Journal of the American Chemical Society

2023 Volume 145, Issue 34, Page(s) 18812–18824

Abstract: Glycan is a crucial class of biological macromolecules with important biological functions. Functional groups determine the chemical properties of glycans, which further affect their biological activities. However, the structural complexity of glycans ... ...

Abstract	Glycan is a crucial class of biological macromolecules with important biological functions. Functional groups determine the chemical properties of glycans, which further affect their biological activities. However, the structural complexity of glycans has set a technical hurdle for their direct identification. Nanopores have emerged as highly sensitive biosensors that are capable of detecting and characterizing various analytes. Here, we identified the functional groups on glycans with a designed α-hemolysin nanopore containing arginine mutations (M113R), which is specifically sensitive to glycans with acetamido and carboxyl groups. Molecular dynamics simulations indicated that the acetamido and carboxyl groups of the glycans produce unique electrical signatures by forming polar and electrostatic interactions with the M113R nanopores. Using these electrical features as the fingerprints, we mapped the length of the glycans containing acetamido and carboxyl groups at the monosaccharide, disaccharide, and trisaccharide levels. This proof-of-concept study provides a promising foundation for developing single-molecule glycan fingerprinting libraries and demonstrates the capability of biological nanopores in glycan sequencing.
MeSH term(s)	Hemolysin Proteins/chemistry ; Nanopores ; Molecular Dynamics Simulation
Chemical Substances	Hemolysin Proteins
Language	English
Publishing date	2023-08-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021/jacs.3c03563
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Article: Chemometric discrimination of the geographical origin of licorice in China by untargeted metabolomics

Lu, Youyuan / Yao, Guangda / Wang, Xuan / Zhang, Yang / Zhao, Jianjun / Yu, Yong-Jie / Wang, Hanqing

Food chemistry. 2022 June 30, v. 380

2022

Abstract: Licorice is famous as a herbal medicine and food sweetener. This study provided a comprehensive strategy for investigating the quality of licorice based on untargeted metabolomics. A new strategy for identifying metabolite was developed, including ... ...

Abstract	Licorice is famous as a herbal medicine and food sweetener. This study provided a comprehensive strategy for investigating the quality of licorice based on untargeted metabolomics. A new strategy for identifying metabolite was developed, including fragment ion identification algorithm and ion fusion algorithm. The results showed that it can accurately integrate mass spectra from positive and negative ion modes to benefit metabolite identification. Based on the strategy, a number of significant difference metabolites were identified among licorice samples and 9 metabolites were confirmed by standards. Additionally, the geographical discrimination models of licorice samples were comprehensively investigated by chemometric methods. The results indicated that the supporting vector machine provided the best performance, with a prediction accuracy above 80%. The study results supported the conclusion that the quality of licorice from different regions in China was inconsistent.
Keywords	algorithms ; chemometrics ; food chemistry ; herbal medicines ; licorice ; metabolites ; metabolomics ; prediction ; provenance ; China
Language	English
Dates of publication	2022-0630
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2022.132235
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: 14-3-3ζ Plays a key role in the modulation of neuroplasticity underlying the antidepressant-like effects of Zhi-Zi-Chi-Tang

Tao, Weiwei / Yao, Guangda / Yue, Qiyu / Xu, Chunyan / Hu, Yue / Cheng, Xiaolan / Zhao, Tong / Qi, Mingming / Chen, Gang / Zhao, Min / Yu, Yue

Phytomedicine. 2023 July, v. 116 p.154888-

2023

Abstract: Zhi-Zi-Chi-Tang (ZZCT) is an effective traditional Chinese medicinal formula. ZZCT has been used for the treatment of depression for centuries. Its clinical efficacy in relieving depression has been confirmed. However, the molecular mechanisms of ZZCT ... ...

Abstract	Zhi-Zi-Chi-Tang (ZZCT) is an effective traditional Chinese medicinal formula. ZZCT has been used for the treatment of depression for centuries. Its clinical efficacy in relieving depression has been confirmed. However, the molecular mechanisms of ZZCT regarding neuroplasticity in the pathogenesis of depression have not yet been elucidated. The present study aimed to examine the effects of ZZCT on neuroplasticity in mice exposed to chronic unpredictable mild stress (CUMS), and to explore the underlying molecular mechanisms. For this purpose, a murine model of depression was established using the CUMS procedure. Following the intragastric administration of ZZCT or fluoxetine, classic behavioral experiments were performed to observe the efficacy of ZZCT as an antidepressant. Immunofluorescence was used to label and quantify microtubule-associated protein (MAP2) and postsynaptic density protein (PSD95) in the hippocampus. Golgi staining was applied to visualize the dendritic spine density of neurons in the hippocampi. Isolated hippocampal slices were prepared to induce long-term potentiation (LTP) in the CA1 area. The hippocampal protein expression levels of glycogen synthase kinase-3β (GSK-3β), p-GSK-3β (Ser9), cAMP response element binding protein (CREB), p-CREB (Ser133), brain-derived neurotrophic factor (BDNF) and 14–3–3ζ were detected using western blot analysis. The interaction of 14–3–3ζ and p-GSK-3β (Ser9) was examined using co-immunoprecipitation. LV-shRNA was used to knockdown 14–3–3ζ by an intracerebroventricular injection. ZZCT (6 g/kg) and fluoxetine (20 mg/kg) alleviated depressive-like behavior, restored hippocampal MAP2⁺ PSD95⁺ intensity, and reversed the dendritic spine density of hippocampal neurons and LTP in the CA1 region of mice exposed to CUMS. Both low and high doses of ZZCT (3 and 6 g/kg) significantly promoted the binding of 14–3–3ζ to p-GSK-3β (Ser9) in the hippocampus, and ZZCT (6 g/kg) significantly promoted the phosphorylation of GSK-3β Ser9 and CREB Ser133 in the hippocampus. ZZCT (3 and 6 g/kg) upregulated hippocampal BDNF expression in mice exposed to CUMS. LV-sh14–3–3ξ reduced the antidepressant effects of ZZCT. ZZCT exerted antidepressant effects against CUMS-stimulated depressive-like behavior mice. The knockdown of 14–3–3ζ using lentivirus confirmed that 14–3–3ζ was involved in the ZZCT-mediated antidepressant effects through GSK-3β/CREB/BDNF signaling. On the whole, these results suggest that the antidepressant effects of ZZCT are attributed to restoring damage by neuroplasticity enhancement via the 14–3–3ζ/GSK-3β/CREB/BDNF signaling pathway.
Keywords	Lentivirus ; Western blotting ; animal models ; antidepressants ; dendrites ; fluorescent antibody technique ; fluoxetine ; glycogen (starch) synthase ; hippocampus ; intragastric administration ; neuroplasticity ; pathogenesis ; phosphorylation ; precipitin tests ; protein synthesis ; Zhi-Zi-Chi decoction (ZZCT) ; Anti-depression ; 14-3-3ζ ; BDNF ; ZZCT ; CUMS ; TST ; SPT ; FST ; OFT ; PSD95 ; MAP2 ; LTP ; GSK-3β ; CREB ; fEPSP
Language	English
Dates of publication	2023-07
Publishing place	Elsevier GmbH
Document type	Article ; Online
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2023.154888
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Article ; Online: Chemometric discrimination of the geographical origin of licorice in China by untargeted metabolomics.

Lu, Youyuan / Yao, Guangda / Wang, Xuan / Zhang, Yang / Zhao, Jianjun / Yu, Yong-Jie / Wang, Hanqing

Food chemistry

2022 Volume 380, Page(s) 132235

Abstract	Licorice is famous as a herbal medicine and food sweetener. This study provided a comprehensive strategy for investigating the quality of licorice based on untargeted metabolomics. A new strategy for identifying metabolite was developed, including fragment ion identification algorithm and ion fusion algorithm. The results showed that it can accurately integrate mass spectra from positive and negative ion modes to benefit metabolite identification. Based on the strategy, a number of significant difference metabolites were identified among licorice samples and 9 metabolites were confirmed by standards. Additionally, the geographical discrimination models of licorice samples were comprehensively investigated by chemometric methods. The results indicated that the supporting vector machine provided the best performance, with a prediction accuracy above 80%. The study results supported the conclusion that the quality of licorice from different regions in China was inconsistent.
MeSH term(s)	Chemometrics ; Chromatography, High Pressure Liquid ; Glycyrrhiza ; Mass Spectrometry ; Metabolomics
Language	English
Publishing date	2022-01-24
Publishing country	England
Document type	Journal Article
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2022.132235
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Article ; Online: 14-3-3ζ Plays a key role in the modulation of neuroplasticity underlying the antidepressant-like effects of Zhi-Zi-Chi-Tang.

Tao, Weiwei / Yao, Guangda / Yue, Qiyu / Xu, Chunyan / Hu, Yue / Cheng, XiaoLan / Zhao, Tong / Qi, Mingming / Chen, Gang / Zhao, Min / Yu, Yue

Phytomedicine : international journal of phytotherapy and phytopharmacology

2023 Volume 116, Page(s) 154888

Abstract: Background: Zhi-Zi-Chi-Tang (ZZCT) is an effective traditional Chinese medicinal formula. ZZCT has been used for the treatment of depression for centuries. Its clinical efficacy in relieving depression has been confirmed. However, the molecular ... ...

Abstract	Background: Zhi-Zi-Chi-Tang (ZZCT) is an effective traditional Chinese medicinal formula. ZZCT has been used for the treatment of depression for centuries. Its clinical efficacy in relieving depression has been confirmed. However, the molecular mechanisms of ZZCT regarding neuroplasticity in the pathogenesis of depression have not yet been elucidated. Purpose: The present study aimed to examine the effects of ZZCT on neuroplasticity in mice exposed to chronic unpredictable mild stress (CUMS), and to explore the underlying molecular mechanisms. Methods: For this purpose, a murine model of depression was established using the CUMS procedure. Following the intragastric administration of ZZCT or fluoxetine, classic behavioral experiments were performed to observe the efficacy of ZZCT as an antidepressant. Immunofluorescence was used to label and quantify microtubule-associated protein (MAP2) and postsynaptic density protein (PSD95) in the hippocampus. Golgi staining was applied to visualize the dendritic spine density of neurons in the hippocampi. Isolated hippocampal slices were prepared to induce long-term potentiation (LTP) in the CA1 area. The hippocampal protein expression levels of glycogen synthase kinase-3β (GSK-3β), p-GSK-3β (Ser9), cAMP response element binding protein (CREB), p-CREB (Ser133), brain-derived neurotrophic factor (BDNF) and 14-3-3ζ were detected using western blot analysis. The interaction of 14-3-3ζ and p-GSK-3β (Ser9) was examined using co-immunoprecipitation. LV-shRNA was used to knockdown 14-3-3ζ by an intracerebroventricular injection. Results: ZZCT (6 g/kg) and fluoxetine (20 mg/kg) alleviated depressive-like behavior, restored hippocampal MAP2 Conclusion: ZZCT exerted antidepressant effects against CUMS-stimulated depressive-like behavior mice. The knockdown of 14-3-3ζ using lentivirus confirmed that 14-3-3ζ was involved in the ZZCT-mediated antidepressant effects through GSK-3β/CREB/BDNF signaling. On the whole, these results suggest that the antidepressant effects of ZZCT are attributed to restoring damage by neuroplasticity enhancement via the 14-3-3ζ/GSK-3β/CREB/BDNF signaling pathway.
MeSH term(s)	Mice ; Animals ; Glycogen Synthase Kinase 3 beta/metabolism ; Fluoxetine/pharmacology ; Brain-Derived Neurotrophic Factor/metabolism ; 14-3-3 Proteins/metabolism ; 14-3-3 Proteins/pharmacology ; Antidepressive Agents/pharmacology ; Neuronal Plasticity/physiology ; Cyclic AMP Response Element-Binding Protein/metabolism ; Hippocampus ; Stress, Psychological/drug therapy ; Depression/drug therapy ; Depression/metabolism ; Disease Models, Animal
Chemical Substances	Glycogen Synthase Kinase 3 beta (EC 2.7.11.1) ; Fluoxetine (01K63SUP8D) ; Brain-Derived Neurotrophic Factor ; 14-3-3 Proteins ; Antidepressive Agents ; Cyclic AMP Response Element-Binding Protein
Language	English
Publishing date	2023-05-18
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2023.154888
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Article ; Online: Protective effects of liquiritin on UVB-induced skin damage in SD rats.

Li, Yuanjie / Xia, Changbo / Yao, Guangda / Zhang, Xia / Zhao, Jianjun / Gao, Xiaojuan / Yong, Jingjiao / Wang, Hanqing

International immunopharmacology

2021 Volume 97, Page(s) 107614

Abstract: Overexposure to ultraviolet B (UVB) rays can cause damage to the skin. Liquiritin has a variety of pharmacological effects, such as anti-inflammatory and antioxidant. In the present study, the effect of liquiritin on UVB irradiated rat skin was ... ...

Abstract	Overexposure to ultraviolet B (UVB) rays can cause damage to the skin. Liquiritin has a variety of pharmacological effects, such as anti-inflammatory and antioxidant. In the present study, the effect of liquiritin on UVB irradiated rat skin was investigated. Results showed that UVB irradiation caused erythema and wrinkles on the skin surface, as well as thickening and loss of elasticity of the epidermis and a significant increase in the level of ROS in the skin tissue. At the same time, western blot detected an increase in nuclear factor kappa-B (NF-κB) and matrix metalloproteinases (MMPs) and Elisa also detected an increase in pro-inflammatory factors. Therefore, we hypothesized that UVB irradiation-induced damage is associated with inflammation. Interestingly, application of liquiritin to exposed skin of rats reduced the increase in ROS, pro-inflammatory factors, and MMPs caused by UVB irradiation and increased the levels of Sirtuin3 (SIRT3) and Collagen α1. In addition, after intraperitoneal injection of the SIRT3 inhibitor 3-TYP in rats, the protective effect of liquiritin against UVB damage was found to be diminished. These results suggested that promotion of SIRT3 with liquiritin inhibits UVB-induced production of pro-inflammatory mediators, possibly acting through the SIRT3/ROS/NF-κB pathway. In conclusion, this study suggests that liquiritin is an effective drug candidate for the prevention of UVB damage.
MeSH term(s)	Animals ; Humans ; Rats ; Collagen/agonists ; Collagen/metabolism ; Disease Models, Animal ; Flavanones/pharmacology ; Flavanones/therapeutic use ; Glucosides/pharmacology ; Glucosides/therapeutic use ; NF-kappa B/metabolism ; Oxidative Stress/drug effects ; Oxidative Stress/immunology ; Photosensitivity Disorders/etiology ; Photosensitivity Disorders/pathology ; Photosensitivity Disorders/prevention & control ; Proteolysis/drug effects ; Reactive Oxygen Species/metabolism ; Signal Transduction/drug effects ; Signal Transduction/immunology ; Sirtuins/metabolism ; Skin/drug effects ; Skin/immunology ; Skin/pathology ; Skin/radiation effects ; Skin Neoplasms/etiology ; Skin Neoplasms/pathology ; Skin Neoplasms/prevention & control ; Ultraviolet Rays/adverse effects
Chemical Substances	Collagen (9007-34-5) ; Flavanones ; Glucosides ; liquiritin (T0O79T74CD) ; NF-kappa B ; Reactive Oxygen Species ; SIRT3 protein, rat ; Sirtuins (EC 3.5.1.-)
Language	English
Publishing date	2021-04-22
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2043785-7
ISSN	1878-1705 ; 1567-5769
ISSN (online)	1878-1705
ISSN	1567-5769
DOI	10.1016/j.intimp.2021.107614
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In stock of ZB MED Cologne/Königswinter

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In stock of ZB MED Bonn / Germany

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Inter-library loan at ZB MED

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In stock of ZB MED Cologne/Königswinter

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In stock of ZB MED Cologne/Königswinter

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