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Article: [Expression and influencing factors of hepcidin in classical paroxysmal nocturnal hemoglobinuria].

Wang, Y S / Yao, H J / Bo, L J / Zhao, Y P / Zhang, Y L / Zhang, F K

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi

2019 Volume 40, Issue 10, Page(s) 818–821

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Enzyme-Linked Immunosorbent Assay ; Ferritins ; Hemoglobinuria, Paroxysmal ; Hepcidins ; Humans ; Retrospective Studies
Chemical Substances	Hepcidins ; Ferritins (9007-73-2)
Language	Chinese
Publishing date	2019-11-24
Publishing country	China
Document type	Journal Article
ISSN	0253-2727
ISSN	0253-2727
DOI	10.3760/cma.j.issn.0253-2727.2019.10.004
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Article: [Clinical features and laboratory data analysis of Aeromonas bacteremia with hematological diseases].

Xu, C H / Lin, Q S / Lyu, Y X / Zhu, G Q / Tian, Z Y / Wang, C / Sun, F J / Yao, H J

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi

2020 Volume 40, Issue 12, Page(s) 1035–1039

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Aeromonas ; Anti-Bacterial Agents ; Bacteremia ; Data Analysis ; Hematologic Diseases ; Humans ; Retrospective Studies
Chemical Substances	Anti-Bacterial Agents
Language	Chinese
Publishing date	2020-02-05
Publishing country	China
Document type	Journal Article
ISSN	0253-2727
ISSN	0253-2727
DOI	10.3760/cma.j.issn.0253-2727.2019.12.013
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Article: Effects of a biocontrol agent and methyl jasmonate on postharvest diseases of peach fruit and the possible mechanisms involved.

Yao, H J / Tian, S P

Journal of applied microbiology

2005 Volume 98, Issue 4, Page(s) 941–950

Abstract: Aims: To investigate effects of application of 200 micromol l(-1) methyl jasmonate [MeJA (200)] and Cryptococcus laurentii alone or in combination against postharvest diseases (Monilinia fructicola and Penicillium expansum) in peach fruit stored at 25 ... ...

Abstract	Aims: To investigate effects of application of 200 micromol l(-1) methyl jasmonate [MeJA (200)] and Cryptococcus laurentii alone or in combination against postharvest diseases (Monilinia fructicola and Penicillium expansum) in peach fruit stored at 25 and 0 degrees C, and to evaluate the possible mechanisms involved. Methods and results: The efficacy of controlling postharvest diseases by resistance induced in peach fruit treated with MeJA (200) and C. laurentii alone or in combination and the relationship between activities of defence-related enzymes in peach fruit and lesions caused by M. fructicola and P. expansum were examined. At the same time, the effects of MeJA (200) on the population of C. laurentii in the peach wounds and on the mycelial growth of M. fructicola and P. expansumin vitro were investigated. The results indicated that treatment of peach fruit with C. laurentii at 1 x 10(8) CFU ml(-1) alone, or combining C. laurentii at 5 x 10(7) CFU ml(-1) with MeJA (200) all resulted in a lower lesion diameter of brown rot and blue mould caused by M. fructicola and P. expansum compared with the controls in peach fruit. MeJA (200) enhanced the population of C. laurentii, and inhibited mycelial growth of P. expansum. However, it had a little effect on M. fructicolain vitro. MeJA and C. laurentii alone or in combination induced higher activities of Chitinase, beta-1,3-glucanase, phenylalanine ammonia-lyase and peroxidase (POD) than applying the yeast alone at both 25 and 0 degrees C. Conclusions: MeJA (200) not only directly inhibited mycelial spread of postharvest pathogens, but also increased population of C. laurentii, which induced stronger disease resistance in fruit than MeJA or yeast alone, and resulted in a lower lesion diameter of brown rot and blue mould caused by M. fructicola and P. expansum. Significance and impact of the study: MeJA (200) in combination with C. laurentii was beneficial for controlling brown rot and blue mould caused by M. fructicola and P. expansum in peach fruit. The inhibitory mechanism was mainly because of resistance induced in peach fruit by MeJA and C. laurentii. In addition, direct inhibition of MeJA on P. expansum also played a role in controlling blue mould.
MeSH term(s)	Acetates/pharmacology ; Agriculture ; Ascomycota ; Chitinases/metabolism ; Cryptococcus/drug effects ; Cryptococcus/growth & development ; Cyclopentanes/pharmacology ; Glucan 1,3-beta-Glucosidase/metabolism ; Mycelium/drug effects ; Mycelium/growth & development ; Oxylipins ; Penicillium ; Pest Control, Biological/methods ; Plant Diseases ; Plant Growth Regulators/pharmacology ; Population Dynamics ; Prunus/drug effects ; Prunus/enzymology ; Prunus/microbiology ; Seasons
Chemical Substances	Acetates ; Cyclopentanes ; Oxylipins ; Plant Growth Regulators ; methyl jasmonate (900N171A0F) ; Chitinases (EC 3.2.1.14) ; Glucan 1,3-beta-Glucosidase (EC 3.2.1.58)
Language	English
Publishing date	2005
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1358023-1
ISSN	1365-2672 ; 1364-5072
ISSN (online)	1365-2672
ISSN	1364-5072
DOI	10.1111/j.1365-2672.2004.02531.x
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Article ; Online: Comparison of different approaches to the surgical treatment of penile fractures: quicker return to sexual function with longitudinal incisions.

Xu, M X / Zhou, Z / Yao, H J / Zhang, K / Da, J / Zhang, M / Wang, Z / Lu, M J

International journal of impotence research

2016 Volume 28, Issue 4, Page(s) 155–159

Abstract: The objective of this study was to compare the long-term clinical outcomes from longitudinal incisions and subcoronal circumferential degloving incisions in the surgical treatment of penile fractures. From July 2001 to July 2014, 23 patients were ... ...

Abstract	The objective of this study was to compare the long-term clinical outcomes from longitudinal incisions and subcoronal circumferential degloving incisions in the surgical treatment of penile fractures. From July 2001 to July 2014, 23 patients were identified with penile fractures. Fourteen patients underwent longitudinal incisions after ultrasound localization; nine patients underwent subcoronal circumferential degloving incisions. Sexual function was evaluated preoperatively and postoperatively using an abridged International Index of Erectile Function (IIEF) questionnaire. The mean (±s.d.) operative time was 19.1 (±3.9) min in the longitudinal incision group and was 45.1 (±6.5) min in the subcoronal circumferential degloving incision group (P<0.05). The mean (±s.d.) times required to recover sexual function were 35.6 (±6.0) days in the longitudinal incision group and 54.0 (±5.8) days in the circumferential incision group (P<0.05). Six months postoperatively, the erectile functions of all cases were comparable to the level preoperatively except three patients. One patient from each group reported symptoms associated with mild ED, but they experienced satisfying sexual orgasms after psychotherapy for 2 months. Another patient's score on the IIEF-5 declined from 25 to 24 points in the circumferential incision group 10 months postoperatively, and this was associated with maintaining an erection after vaginal penetration. In conclusion, the longitudinal incision may allow quicker return to sexual function but not necessarily improved the long-term clinical outcomes. Furthermore, postoperative psychosocial nursing and psychotherapy should receive more attention.
MeSH term(s)	Adult ; Coitus/physiology ; Humans ; Male ; Middle Aged ; Penile Diseases/surgery ; Penile Erection/physiology ; Penis/injuries ; Penis/surgery ; Personal Satisfaction ; Postoperative Care ; Treatment Outcome ; Urologic Surgical Procedures, Male/methods
Language	English
Publishing date	2016-07
Publishing country	England
Document type	Journal Article
ZDB-ID	1034295-3
ISSN	1476-5489 ; 0955-9930
ISSN (online)	1476-5489
ISSN	0955-9930
DOI	10.1038/ijir.2016.13
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Article: Effects of a biocontrol agent and methyl jasmonate on postharvest diseases of peach fruit and the possible mechanisms involved

Yao, H.J / Tian, S.P

Journal of applied microbiology. 2005, v. 98, no. 4

2005

Keywords	peaches ; fruit diseases ; postharvest diseases ; fungal diseases of plants ; Monilinia fructicola ; Penicillium expansum ; plant pathogenic fungi ; disease control ; biological control ; Cryptococcus laurentii ; biological control agents ; fungal antagonists ; methyl jasmonate
Language	English
Size	p. 941-950.
Document type	Article
ZDB-ID	1358023-1
ISSN	1364-5072
ISSN	1364-5072
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Effective extraction and purification of beta-xylosidase from Trichoderma koningii fermentation culture by aqueous two-phase partitioning.

Pan, I -H. / Yao, H -J. / Li, Y -K.

Enzyme and microbial technology

2001 Volume 28, Issue 2-3, Page(s) 196–201

Abstract: Effective extraction of protein from bulk medium is an important technique in bioresearch. In the present study, we describe an extracellular beta-xylosidase from the fermentation supernatant of Trichoderma koningii G-39 that was successfully extracted ... ...

Abstract	Effective extraction of protein from bulk medium is an important technique in bioresearch. In the present study, we describe an extracellular beta-xylosidase from the fermentation supernatant of Trichoderma koningii G-39 that was successfully extracted and purified simultaneously in a single step by using an aqueous two-phase partitioning method. This two-phase system was prepared by dissolving suitable amount of poly(ethylene glycol) (PEG) and sodium dihydrogenphosphate (NaH(2)PO(4)) in aqueous solution. beta-Xylosidase was recovered with high yield and high concentration in the bottom salt-rich phase when 25% (w/v) PEG 1500 and 20-25% (w/v) NaH(2)PO(4) were applied. Based on a 1-liter scale extraction, the purity of the enzyme was enhanced at least 33-fold. The total activity increased 422% in comparison with that in the untreated filtrate. The effectiveness and simplicity may make this technique potentially useful in various applications. The transxylosylation activity of the enzyme purified by this technique was also investigated.
Language	English
Publishing date	2001-02-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	423729-8
ISSN	1879-0909 ; 0141-0229
ISSN (online)	1879-0909
ISSN	0141-0229
DOI	10.1016/s0141-0229(00)00291-x
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Article: Transcriptional mapping in Chilo iridescent virus infections.

D'Costa, S M / Yao, H J / Bilimoria, S L

Archives of virology

2004 Volume 149, Issue 4, Page(s) 723–742

Abstract: Chilo iridescent virus (CIV) belongs to the family Iridoviridae, which are icosahedral cytoplasmic DNA viruses with large, linear, and circularly permuted genomes. Previous studies on infected-cell-specific polypeptides suggested temporal regulation of ... ...

Abstract	Chilo iridescent virus (CIV) belongs to the family Iridoviridae, which are icosahedral cytoplasmic DNA viruses with large, linear, and circularly permuted genomes. Previous studies on infected-cell-specific polypeptides suggested temporal regulation of CIV gene expression. Recently, we demonstrated three temporal classes at the transcriptional level, in CIV infections of a spruce budworm cell line. We also demonstrated a transcriptional cascade with positive and negative control. In this paper, we assign all detectable viral transcripts into respective temporal classes and map them using restriction fragments from a genomic library. More than 90 percent of the genome is transcriptionally active with at least four major clusters of immediate-early transcription and at least three delayed-early clusters. Late transcripts were observed throughout the genome. There was at least one exclusive region in the genome for each of the three temporal classes. We correlated transcribed regions with ORFs on the CIV genome and showed that known ORFs in the exclusive regions are generally consistent with phase-specific requirements of large DNA viruses. Our data also suggest the presence of 5' or 3' coterminal transcripts. This is the first complete transcription map for a member of the genus Iridovirus.
MeSH term(s)	Animals ; Cell Line ; Deoxyribonuclease EcoRI ; Genome, Viral ; Iridoviridae/genetics ; Iridoviridae/metabolism ; Moths ; RNA, Messenger/biosynthesis ; RNA, Viral/biosynthesis ; RNA, Viral/chemistry ; RNA, Viral/genetics ; Restriction Mapping
Chemical Substances	RNA, Messenger ; RNA, Viral ; Deoxyribonuclease EcoRI (EC 3.1.21.-)
Language	English
Publishing date	2004-04
Publishing country	Austria
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	7491-3
ISSN	1432-8798 ; 0304-8608
ISSN (online)	1432-8798
ISSN	0304-8608
DOI	10.1007/s00705-003-0232-4
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Article: Mechanistic study of beta-xylosidase from Trichoderma koningii G-39.

Li, Y K / Yao, H J / Pan, I H

Journal of biochemistry

2000 Volume 127, Issue 2, Page(s) 315–320

Abstract: The catalytic mechanism of the beta-xylosidase purified from the culture filtrate of Trichoderma koningii G-39 was investigated. By NMR spectroscopy, the stereochemistry of the enzyme catalyzing the hydrolysis of 2,4-dinitrophenyl and p-nitrophenyl-beta- ... ...

Abstract	The catalytic mechanism of the beta-xylosidase purified from the culture filtrate of Trichoderma koningii G-39 was investigated. By NMR spectroscopy, the stereochemistry of the enzyme catalyzing the hydrolysis of 2,4-dinitrophenyl and p-nitrophenyl-beta-D-xylosides was found unequivocally to involve retention of the anomeric configuration. Based on the k(cat) values of a series of arylxylosides with leaving group pK(a)s in the range of 4-10, an extended Bronsted plot was constructed with a slope (beta(lg)) near zero. Enzymatic hydrolysis of aryl-beta-D-xylosides in acetate buffer (pH 4.0) containing 3 or 5% methanol showed a constant product ratio (methylxyloside/xylose), indicating the presence of a common intermediate, probably the xylosyl-enzyme intermediate. In the presence of DTT, the k(cat) values of p-cyanophenyl-beta-D-xylopyranoside and p-nitrophenyl-beta-D-xylopyranoside increased greatly. A two-step mechanism involving the formation and breakdown of the xylosyl-enzyme intermediate was therefore proposed. The rate-limiting step is the breakdown of the intermediate. The secondary deuterium kinetic isotope effect (k(H)/k(D)) measured for 2,4-dinitrophenyl-beta-D-xyloside was 1.02+/-0.01, suggesting that the transition state for breakdown of the xylosyl-enzyme intermediate is S(N)2-like.
MeSH term(s)	Binding, Competitive ; Deuterium ; Dithiothreitol/pharmacology ; Enzyme Inhibitors/pharmacology ; Gluconates/pharmacology ; Glycosides/metabolism ; Kinetics ; Lactones ; Magnetic Resonance Spectroscopy ; Mechanics ; Pyrones/pharmacology ; Stereoisomerism ; Substrate Specificity ; Trichoderma/enzymology ; Xylose/analogs & derivatives ; Xylose/pharmacology ; Xylosidases/antagonists & inhibitors ; Xylosidases/chemistry ; Xylosidases/metabolism
Chemical Substances	Enzyme Inhibitors ; Gluconates ; Glycosides ; Lactones ; Pyrones ; delta-xylonolactone ; xylosides ; Xylose (A1TA934AKO) ; Deuterium (AR09D82C7G) ; Xylosidases (EC 3.2.1.-) ; exo-1,4-beta-D-xylosidase (EC 3.2.1.37) ; Dithiothreitol (T8ID5YZU6Y) ; beta-glucono-1,5-lactone (WQ29KQ9POT)
Language	English
Publishing date	2000-02
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	218073-x
ISSN	1756-2651 ; 0021-924X
ISSN (online)	1756-2651
ISSN	0021-924X
DOI	10.1093/oxfordjournals.jbchem.a022609
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Article: Effective induction, purification and characterization of Trichoderma koningii G-39 beta-xylosidase with high transferase activity.

Li, Y K / Yao, H J / Cho, Y t

Biotechnology and applied biochemistry

2000 Volume 31, Issue 2, Page(s) 119–125

Abstract: A beta-xylosidase was induced and purified from the culture filtrate of Trichoderma koningii G-39, grown in a medium containing 1% oat spelts xylan and 0.1% xylose. The presence of xylose unequivocally enhanced the induction of beta-xylosidase. The ... ...

Abstract	A beta-xylosidase was induced and purified from the culture filtrate of Trichoderma koningii G-39, grown in a medium containing 1% oat spelts xylan and 0.1% xylose. The presence of xylose unequivocally enhanced the induction of beta-xylosidase. The purified enzyme, which exhibited a significant alpha-arabinosidase activity, was obtained with high yield simply via ethanol precipitation and a single anion-exchange chromatography and was characterized as a monomeric glycoprotein with an estimated molecular mass of 104 kDa and a pI of 4.6. The K(m) values towards p-nitrophenyl beta-D-xylopyranoside and p-nitrophenyl alpha-L-arabinopyranoside are 0.04 and 7.5 mM, respectively. It is stable at pH 2.5-7.4, 37 degrees C. The pH and temperature optima are in the range of 3.5-4.0 and 55-60 degrees C, respectively. Contrary to most beta-xylosidases from other sources, Hg(2+) (up to 25 mM) has no effect on enzyme activity. Xylose was shown to inhibit the purified enzyme with a moderate K(i) value of 5 mM. The enzyme exhibited transxylosylation activity and was characterized as a 'retaining' enzyme, catalysing the hydrolysis of substrate with the retention of anomeric configuration.
MeSH term(s)	Biotechnology ; Enzyme Induction ; Enzyme Inhibitors/pharmacology ; Enzyme Stability ; Isoelectric Point ; Kinetics ; Magnetic Resonance Spectroscopy ; Metals/pharmacology ; Molecular Weight ; Substrate Specificity ; Temperature ; Trichoderma/enzymology ; Xylosidases/biosynthesis ; Xylosidases/chemistry ; Xylosidases/isolation & purification
Chemical Substances	Enzyme Inhibitors ; Metals ; Xylosidases (EC 3.2.1.-) ; exo-1,4-beta-D-xylosidase (EC 3.2.1.37)
Language	English
Publishing date	2000
Publishing country	United States
Document type	Journal Article
ZDB-ID	883433-7
ISSN	0885-4513
ISSN	0885-4513
DOI	10.1042/ba19990072
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Article: Transcriptional mapping in Chilo iridescent virus infections

D'Costa, S.M / Yao, H.J / Bilimoria, S.L

Archives of virology. 2004 Apr., v. 149, no. 4

2004

Keywords	Invertebrate iridescent virus 6 ; transcription (genetics) ; gene expression regulation ; genome
Language	English
Dates of publication	2004-04
Size	p. 723-742.
Document type	Article
ZDB-ID	7491-3
ISSN	1432-8798 ; 0304-8608
ISSN (online)	1432-8798
ISSN	0304-8608
Database	NAL-Catalogue (AGRICOLA)

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