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  1. Article ; Online: Highly effective heterogeneous doxycycline stabilized silver nanocatalyst for the degradation of ibuprofen and paracetamol drugs

    Yasmeen Junejo / Muhammad Safdar

    Arabian Journal of Chemistry, Vol 12, Iss 8, Pp 2823-

    2019  Volume 2832

    Abstract: In this work, a new doxycycline stabilized silver nanocatalyst (Dox-Ag (0) NPs) was synthesized in aqueous solution (green method) by one-pot simple synthetic method for the ultra-fast catalytic degradation of ibuprofen and paracetamol. The formation of ... ...

    Abstract In this work, a new doxycycline stabilized silver nanocatalyst (Dox-Ag (0) NPs) was synthesized in aqueous solution (green method) by one-pot simple synthetic method for the ultra-fast catalytic degradation of ibuprofen and paracetamol. The formation of the Dox-Ag (0) NPs was monitored using UV–Vis absorption spectroscopy which confirmed the formation of Dox-Ag (0) NPs by exciting the typical surface plasmon absorption maxima at 404 nm. Transmission electron microscopy (TEM) confirmed the spherical morphology and monodispersed Dox-Ag (0) NPs with particle size 6.87 ± 2.2 nm. The newly synthesized Dox-Ag (0) NPs had an excellent catalytic activity as a catalyst for the 100% degradation of ibuprofen and paracetamol, which was carried out in 60 s. The antimicrobial activities of this catalyst were also evaluated against Gram-negative bacteria Mycoplasma hominis, Escherichia coli, Pseudomonas aeruginosa and Gram-positive bacteria Staphylococcus aureus, Micrococcus flavus and Micrococcus luteus by the disk diffusion method. Whereas standard antibiotic showed no zone of inhibition, the Dox-Ag (0) NPs showed good inhibition zone. The antimicrobial results therefore reveal that newly synthesized Dox-Ag (0) NPs had a tremendous catalytic and antimicrobial activity as a catalyst. They were recovered easily from reaction medium and reused with enhanced catalytic potential seven times. The current findings are equally extendable for safeguarding the aquatic environment against the pollution caused by drugs and microbial activity via a facile, highly economical, rapid and efficient reduction/degradation method based on the catalytic potential of Dox-Ag (0) NPs. Keywords: Doxycycline stabilized silver nanocatalyst, Heterogeneous catalysis, Green method, Drugs degradation, Antimicrobial activity
    Keywords Chemistry ; QD1-999
    Subject code 540
    Language English
    Publishing date 2019-12-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: The development of a hexaplex-conventional PCR for identification of six animal and plant species in foodstuffs

    Safdar, Muhammad / Yasmeen Junejo

    Food chemistry. 2016 Feb. 01, v. 192

    2016  

    Abstract: A hexaplex-conventional PCR assay was developed for identification of five meat and one plant species origins in foodstuffs simultaneously. The method merges the use of horse (Equus caballus), soybean (Glycine max), sheep (Ovis aries), poultry (Meleagris ...

    Abstract A hexaplex-conventional PCR assay was developed for identification of five meat and one plant species origins in foodstuffs simultaneously. The method merges the use of horse (Equus caballus), soybean (Glycine max), sheep (Ovis aries), poultry (Meleagris meleagris), pork (Sus scrofa), and cow (Bos taurus) specific primers that amplify fragments (horse; 85bp, soybean; 100bp, sheep; 119bp, poultry; 183bp, pork; 212bp and cow; 271bp) of the mitochondrial cyt b, lectin, 12S rRNA, 12S rRNA, ATPase subunit 6 genes and ATPase subunit 8 genes respectively, and a universal 18S rRNA primers that amplifies a 141bp. Multiplex analysis of the reference food samples showed that detection limit of the hexaplex assay was 0.01% for each species. Taken together, all data indicated that this hexaplex PCR assay was a simple, fast, sensitive, specific, and cost-effective detection method for horse, soybean, sheep, poultry, pork and cow species in foodstuffs.
    Keywords adenosinetriphosphatase ; cost effectiveness ; cows ; detection limit ; genes ; Glycine max ; horses ; lectins ; Meleagris ; mitochondria ; polymerase chain reaction ; pork ; poultry ; ribosomal RNA ; sheep ; soybeans ; Sus scrofa
    Language English
    Dates of publication 2016-0201
    Size p. 745-749.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 243123-3
    ISSN 1873-7072 ; 0308-8146
    ISSN (online) 1873-7072
    ISSN 0308-8146
    DOI 10.1016/j.foodchem.2015.07.082
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  3. Article: A multiplex-conventional PCR assay for bovine, ovine, caprine and fish species identification in feedstuffs: Highly sensitive and specific

    Safdar, Muhammad / Yasmeen Junejo

    Food control. 2015 Apr., v. 50

    2015  

    Abstract: A multiplex PCR assay was developed for rapid and reliable identification of bovine, ovine, caprine and fish species in feedstuffs simultaneously. The method merges the use of bovine, ovine, caprine and fish primers that amplify fragments (ovine; 119 bp, ...

    Abstract A multiplex PCR assay was developed for rapid and reliable identification of bovine, ovine, caprine and fish species in feedstuffs simultaneously. The method merges the use of bovine, ovine, caprine and fish primers that amplify fragments (ovine; 119 bp, caprine; 142 bp, fish; 224 bp and bovine; 271 bp) of the mitochondrial t.glu gene forward and cyt b reverse, 12S rRNA, 12S rRNA, and ATPase subunit 8 genes respectively, and a universal 18S rRNA primers that amplifies a 99 bp from eukaryotic DNA. To evaluate the effect of heat treatment, a severe sterilization condition (133 °C at 300 kPa for 20 min) was applied. Multiplex analysis of the reference feedstuff samples showed that the detection limit of the assay was 0.01% for each species. Taken together, all data indicated that this multiplex PCR assay was a simple, rapid, sensitive, specific, and cost-effective detection method for bovine, ovine, caprine and fish species in feedstuffs.
    Keywords adenosinetriphosphatase ; cattle ; cost effectiveness ; detection limit ; DNA ; feeds ; fish ; genes ; goats ; heat treatment ; mitochondria ; polymerase chain reaction ; ribosomal RNA ; sheep ; species identification
    Language English
    Dates of publication 2015-04
    Size p. 190-194.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1027805-9
    ISSN 0956-7135
    ISSN 0956-7135
    DOI 10.1016/j.foodcont.2014.08.048
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 5255: Show issues

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  4. Article ; Online: Facile Synthesis, Characterization and Catalytic Function of Gelatin Stabilized Gold Nanoparticles

    Zulfiqar A. Tagar / Sirajuddin / Najma Memon / Muhammad S. Kalhoro / Nazar H. Kalwar / Yasmeen Junejo / Syeda Sara Hassan

    Pakistan Journal of Analytical & Environmental Chemistry, Vol 13, Iss 01, Pp 70-

    2012  Volume 77

    Abstract: In the present investigation, we report a new one phase, one pot method for synthesis of 3.5 ± 0.7 nm average sized gelatin capped gold nano particles (Gel-AuNPs) using strong reductant NaBH4 in aqueous system at room temperature. Size controlled Gel- ... ...

    Abstract In the present investigation, we report a new one phase, one pot method for synthesis of 3.5 ± 0.7 nm average sized gelatin capped gold nano particles (Gel-AuNPs) using strong reductant NaBH4 in aqueous system at room temperature. Size controlled Gel-AuNPs were characterized by UV-Visible, STEM, XRD, DLS and FT-IR. Spherical Gel-AuNPs showed excellent catalytic activity for reduction of three differently charged dyes eosin B (EB), methylene blue (MB) and rose bengal (RB) in the presence of NaBH4. The study revealed that 100% reduction of EB, MB and RB dyes was carried out in just 150 sec. The developed catalyst was easy to recover and capable to be reused three times. The process of reduction rate and kinetics of dyes was monitored using UV-Visible spectrophotometer. A plot of lnC Vs time (sec) showed that reaction follows the first order kinetics. Rate constant (K) was determined for EB, MB and RB reduction at 10 μM, which was found as 2.735 x10-2 sec-1, 2.738 x 10-2 sec-1 and 2.55 x 10-2 sec-1, respectively. The study revealed that catalytic reduction of dyes by Gel-AuNPs in aqueous medium is environmental friendly in terms of recovery of catalyst, is exceptionally fast and hence extremely economical.
    Keywords Gelatin ; Controlled ; Catalytic activity ; Dyes ; Recovery ; Environmental sciences ; GE1-350 ; Analytical chemistry ; QD71-142
    Subject code 540
    Language English
    Publishing date 2012-06-01T00:00:00Z
    Publisher National Centre of Excellence in Analytical Chemsitry
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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