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  1. AU="Ye, Guangming"
  2. AU="Yiyi, L"
  3. AU="Siles, Francisco"
  4. AU="Song, Sin-Mao"
  5. AU="Yaxuan He"
  6. AU="Wu, Jiaojie"
  7. AU="Tze Kwun Ng"
  8. AU="Leonard L Yeo"

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  1. Article ; Online: Highly sensitive and specific graphene oxide-based FRET aptasensor for quantitative detection of human soluble growth stimulating gene protein 2.

    Zeng, Zhikun / Li, Wenfeng / Zhang, Jixuan / Hu, Zijian / Wu, Junyi / Ye, Guangming / Luo, Yi

    Talanta

    2024  Volume 271, Page(s) 125629

    Abstract: Soluble growth stimulation expressed gene 2 (sST2) is a new generation biomarker in the diagnosis and prognosis of heart failure (HF). Here, the sST2-specific aptamers were selected from a random ssDNA library with the full length of 88 nucleotides (nt) ... ...

    Abstract Soluble growth stimulation expressed gene 2 (sST2) is a new generation biomarker in the diagnosis and prognosis of heart failure (HF). Here, the sST2-specific aptamers were selected from a random ssDNA library with the full length of 88 nucleotides (nt) via target-immobilized magnetic beads (MB)-based systematic evolution of ligands by exponential enrichment (SELEX) technology. After eight rounds of selection, six aptamers with the most enrichment were selected. Among, the aptamer L1 showed the high-affinity binding to sST2 with the lowest Kd value (77.3 ± 0.05 nM), which was chosen as the optimal aptamer for further molecular docking. Then, the aptamer L1 was used to construct a graphene oxide (GO) - based fluorescence resonance energy transfer (FRET) biosensor for sST2, which exhibits a linear detection range of 0.1-100 μg/ml and a detection limit of 3.7 ng/ml. The aptasensor was applied to detect sST2 in real samples, with a good correlation and agreement with the traditional enzyme-linked immunosorbent assay (ELISA) when quantitative analyzing the sST2 concentration in serum samples from HF patients. The results show that not only an efficient strategy for screening the practicable aptamer, but also a rapid and sensitive detection platform for sST2 were established.
    MeSH term(s) Humans ; Aptamers, Nucleotide/genetics ; Chromatography, Affinity ; DNA, Single-Stranded ; Fluorescence Resonance Energy Transfer ; Graphite ; Heart Failure/diagnosis ; Molecular Docking Simulation ; Oligonucleotides ; SELEX Aptamer Technique ; Biomarkers/analysis ; Receptors, Interleukin-1/analysis
    Chemical Substances Aptamers, Nucleotide ; DNA, Single-Stranded ; graphene oxide ; Graphite (7782-42-5) ; Oligonucleotides ; Biomarkers ; IL1RL1 protein, human ; Receptors, Interleukin-1
    Language English
    Publishing date 2024-01-09
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1500969-5
    ISSN 1873-3573 ; 0039-9140
    ISSN (online) 1873-3573
    ISSN 0039-9140
    DOI 10.1016/j.talanta.2024.125629
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  2. Article ; Online: Systematic alanine and stapling mutational analysis of antimicrobial peptide Chem-KVL.

    Zhen, Borui / Geng, Chenchen / Yang, Yi / Liang, Haiyan / Jiang, Yunyun / Li, Xiang / Ye, Guangming

    Bioorganic & medicinal chemistry letters

    2024  Volume 107, Page(s) 129794

    Abstract: Chem-KVL is a tandem repeating peptide, with 14 amino acids that was modified based on a short peptide from a fragment of the human host defense protein chemerin. Chem-KVL increases cationicity and hydrophobicity and shows broad-spectrum antibacterial ... ...

    Abstract Chem-KVL is a tandem repeating peptide, with 14 amino acids that was modified based on a short peptide from a fragment of the human host defense protein chemerin. Chem-KVL increases cationicity and hydrophobicity and shows broad-spectrum antibacterial activity. To determine the molecular determinants of Chem-KVL and whether staple-modified Chem-KVL would improve antibacterial activity and protease stability or decrease cytotoxicity, we combined alanine and stapling scanning, and designed a series of alanine and staple-derived Chem-KVL peptides, termed Chem-A1 to Chem-A14 and SCL-1 to SCL-7. We next examined their antibacterial activity against several gram-positive and gram-negative bacteria, their proteolytic stability, and their cytotoxicity. Ala scanning of Chem-KVL suggested that both the positively charged residues (Lys and Arg) and the hydrophobic residues (Lue and Val) were critical for the antibacterial activities of Chem-KVL peptide. Of note, Chem-A4 was able to remarkably inhibit the growth of gram-positive and gram-negative bacteria when compared to the original peptide. And the antibacterial activities of stapled SCL-4 and SCL-7 were several times higher than those of the linear peptide against gram-positive and gram-negative bacteria. Stapling modification of peptides resulted in increased helicity and protein stability when compared with the linear peptide. These stapled peptides, especially SCL-4 and SCL-7, may serve as the leading compounds for further optimization and antimicrobial therapy.
    Language English
    Publishing date 2024-05-10
    Publishing country England
    Document type Journal Article
    ZDB-ID 1063195-1
    ISSN 1464-3405 ; 0960-894X
    ISSN (online) 1464-3405
    ISSN 0960-894X
    DOI 10.1016/j.bmcl.2024.129794
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  3. Article ; Online: Microarray analysis for the identification of specific proteins and functional modules involved in the process of hepatocellular carcinoma originating from cirrhotic liver.

    Fan, Wufeng / Ye, Guangming

    Molecular medicine reports

    2018  Volume 17, Issue 4, Page(s) 5619–5626

    Abstract: In order to identify the potential pathogenesis of hepatocellular carcinoma (HCC) developing from cirrhosis, a microarray‑based transcriptome profile was analyzed. The GSE63898 expression profile was downloaded from the Gene Expression Omnibus database, ... ...

    Abstract In order to identify the potential pathogenesis of hepatocellular carcinoma (HCC) developing from cirrhosis, a microarray‑based transcriptome profile was analyzed. The GSE63898 expression profile was downloaded from the Gene Expression Omnibus database, which included data from 228 HCC tissue samples and 168 cirrhotic tissue samples. The Robust Multi‑array Average in the Affy package of R was used for raw data processing and Student's t‑test was used to screen differentially expressed genes (DEGs). An enrichment analysis was then conducted using the Database for Annotation, Visualization and Integrated Discovery online tool, and the protein‑protein interaction (PPI) network was constructed using the Search Tool for the Retrieval of Interacting Genes and Cytoscape. Furthermore, the MCODE plug‑in of Cytoscape was used to conduct a sub‑module analysis. A total of 634 DEGs were identified between HCC and cirrhosis, of which 165 were upregulated and 469 were downregulated. According to the cut‑off criteria, the PPI network was constructed and Jun proto‑oncogene, AP‑1 transcription factor subunit (degree, 39), Fos proto‑oncogene, AP‑1 transcription factor subunit (degree, 34) and v‑myc avian myelocytomatosis viral oncogene homolog (degree, 32) were identified as the hub nodes of the PPI network. Based on the sub‑module analysis, four specific modules were identified. In particular, module 1 was significantly enriched in the chemokine signaling pathway, and C‑X‑C motif chemokine ligand 12, C‑C motif chemokine receptor 7 (CCR7) and C‑C motif chemokine ligand 5 (CCL5) were three important proteins in this module. Module 4 was significantly enriched in chemical carcinogenesis, and cytochrome P450 family 2 subfamily E member 1, cytochrome P450 family 2 subfamily C member 9 (CYP2C9) and cytochrome P450 family 2 subfamily A member 6 (CYP2A6) were three important proteins in this module. In conclusion, the present study revealed that CCR7, CCL5, CYP2C9 and CYP2A6 are novel genes identified in the development of HCC; however, the actual functions of these genes require verification.
    MeSH term(s) Carcinoma, Hepatocellular/etiology ; Carcinoma, Hepatocellular/metabolism ; Carcinoma, Hepatocellular/pathology ; Computational Biology/methods ; Databases, Genetic ; Gene Expression Profiling ; Gene Regulatory Networks ; Humans ; Liver Cirrhosis/complications ; Liver Neoplasms/etiology ; Liver Neoplasms/metabolism ; Liver Neoplasms/pathology ; Microarray Analysis ; Protein Interaction Mapping ; Protein Interaction Maps ; Proteome ; Proteomics/methods ; Transcriptome
    Chemical Substances Proteome
    Language English
    Publishing date 2018-02-02
    Publishing country Greece
    Document type Journal Article
    ZDB-ID 2469505-1
    ISSN 1791-3004 ; 1791-2997
    ISSN (online) 1791-3004
    ISSN 1791-2997
    DOI 10.3892/mmr.2018.8555
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  4. Article ; Online: Family cluster of three recovered cases of pneumonia due to severe acute respiratory syndrome coronavirus 2 infection.

    Xiong, Yong / Song, Shihui / Ye, Guangming / Wang, Xinghuan

    BMJ case reports

    2020  Volume 13, Issue 5

    Abstract: The coronavirus disease (COVID-19) outbreak caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first reported in Wuhan, China, in late 2019 and has affected more than 1 270 000 people worldwide. The numbers of reported cases ... ...

    Abstract The coronavirus disease (COVID-19) outbreak caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first reported in Wuhan, China, in late 2019 and has affected more than 1 270 000 people worldwide. The numbers of reported cases continue to rise and threaten global health. Transmissions among family members are frequently observed, although the route of transmission is partially known. Here we report three cases of SARS-CoV-2 infection within one family. Sequencing of the S gene of the viral genome showed 100% identity among samples, suggesting that the same strain caused the infection. Following treatment with oseltamivir and short-term methylprednisolone combined with symptomatic management, all three patients recovered within 3 weeks, as evidenced by the disappearance of their symptoms, clearance of pulmonary infiltrates and consecutive negative molecular diagnostic test findings. Our observations suggest the importance of preventing family transmission and the efficacy of current integrated treatment for mild/moderate pneumonia in COVID-19 cases.
    MeSH term(s) Adult ; Antiviral Agents/therapeutic use ; Betacoronavirus/genetics ; Betacoronavirus/isolation & purification ; Clinical Laboratory Techniques ; Coronavirus Infections/diagnosis ; Coronavirus Infections/drug therapy ; Coronavirus Infections/transmission ; Family Health ; Female ; Genome, Viral ; Humans ; Male ; Methylprednisolone/therapeutic use ; Middle Aged ; Oseltamivir/therapeutic use ; Pandemics ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/drug therapy ; Pneumonia, Viral/transmission ; Real-Time Polymerase Chain Reaction ; Spike Glycoprotein, Coronavirus/genetics ; Tomography, X-Ray Computed ; Treatment Outcome
    Chemical Substances Antiviral Agents ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2 ; Oseltamivir (20O93L6F9H) ; Methylprednisolone (X4W7ZR7023)
    Keywords covid19
    Language English
    Publishing date 2020-05-04
    Publishing country England
    Document type Case Reports ; Journal Article
    ISSN 1757-790X
    ISSN (online) 1757-790X
    DOI 10.1136/bcr-2020-235302
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  5. Article: High Performance of SARS-Cov-2N Protein Antigen Chemiluminescence Immunoassay as Frontline Testing for Acute Phase COVID-19 Diagnosis: A Retrospective Cohort Study.

    Deng, Qiaoling / Ye, Guangming / Pan, Yunbao / Xie, Wen / Yang, Gui / Li, Zhiqiang / Li, Yirong

    Frontiers in medicine

    2021  Volume 8, Page(s) 676560

    Abstract: Objectives: ...

    Abstract Objectives:
    Language English
    Publishing date 2021-07-14
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2775999-4
    ISSN 2296-858X
    ISSN 2296-858X
    DOI 10.3389/fmed.2021.676560
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  6. Article: Family cluster of three recovered cases of pneumonia due to severe acute respiratory syndrome coronavirus 2 infection

    Xiong, Yong / Song, Shihui / Ye, Guangming / Wang, Xinghuan

    BMJ case reports

    Abstract: The coronavirus disease (COVID-19) outbreak caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first reported in Wuhan, China, in late 2019 and has affected more than 1 270 000 people worldwide. The numbers of reported cases ... ...

    Abstract The coronavirus disease (COVID-19) outbreak caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first reported in Wuhan, China, in late 2019 and has affected more than 1 270 000 people worldwide. The numbers of reported cases continue to rise and threaten global health. Transmissions among family members are frequently observed, although the route of transmission is partially known. Here we report three cases of SARS-CoV-2 infection within one family. Sequencing of the S gene of the viral genome showed 100% identity among samples, suggesting that the same strain caused the infection. Following treatment with oseltamivir and short-term methylprednisolone combined with symptomatic management, all three patients recovered within 3 weeks, as evidenced by the disappearance of their symptoms, clearance of pulmonary infiltrates and consecutive negative molecular diagnostic test findings. Our observations suggest the importance of preventing family transmission and the efficacy of current integrated treatment for mild/moderate pneumonia in COVID-19 cases.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #32371416
    Database COVID19

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  7. Article ; Online: Family cluster of three recovered cases of pneumonia due to severe acute respiratory syndrome coronavirus 2 infection

    Xiong, Yong / Song, Shihui / Ye, Guangming / Wang, Xinghuan

    BMJ Case Reports

    2020  Volume 13, Issue 5, Page(s) e235302

    Abstract: The coronavirus disease (COVID-19) outbreak caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first reported in Wuhan, China, in late 2019 and has affected more than 1 270 000 people worldwide. The numbers of reported cases ... ...

    Abstract The coronavirus disease (COVID-19) outbreak caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first reported in Wuhan, China, in late 2019 and has affected more than 1 270 000 people worldwide. The numbers of reported cases continue to rise and threaten global health. Transmissions among family members are frequently observed, although the route of transmission is partially known. Here we report three cases of SARS-CoV-2 infection within one family. Sequencing of the S gene of the viral genome showed 100% identity among samples, suggesting that the same strain caused the infection. Following treatment with oseltamivir and short-term methylprednisolone combined with symptomatic management, all three patients recovered within 3 weeks, as evidenced by the disappearance of their symptoms, clearance of pulmonary infiltrates and consecutive negative molecular diagnostic test findings. Our observations suggest the importance of preventing family transmission and the efficacy of current integrated treatment for mild/moderate pneumonia in COVID-19 cases.
    Keywords covid19
    Language English
    Publisher BMJ
    Publishing country uk
    Document type Article ; Online
    ISSN 1757-790X
    DOI 10.1136/bcr-2020-235302
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: IL-6 drives T cell death to participate in lymphopenia in COVID-19.

    Zhou, Xiaoqi / Ye, Guangming / Lv, Yibing / Guo, Yanyan / Pan, Xingfei / Li, Yirong / Shen, Guanxin / He, Yong / Lei, Ping

    International immunopharmacology

    2022  Volume 111, Page(s) 109132

    Abstract: Lymphopenia is a common observation in patients with COVID-19. To explore the cause of T cell lymphopenia in the disease, laboratory results of 64 hospitalized COVID-19 patients were retrospectively analyzed and six patients were randomly selected to ... ...

    Abstract Lymphopenia is a common observation in patients with COVID-19. To explore the cause of T cell lymphopenia in the disease, laboratory results of 64 hospitalized COVID-19 patients were retrospectively analyzed and six patients were randomly selected to trace their changes of T lymphocytes and plasma concentration of IL-6 for the course of disease. Results confirmed that the T-cell lymphopenia, especially CD4
    MeSH term(s) COVID-19 ; Cell Death ; Humans ; Interleukin-6 ; Lymphopenia ; Retrospective Studies ; SARS-CoV-2 ; T-Lymphocytes
    Chemical Substances Interleukin-6
    Language English
    Publishing date 2022-08-08
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2043785-7
    ISSN 1878-1705 ; 1567-5769
    ISSN (online) 1878-1705
    ISSN 1567-5769
    DOI 10.1016/j.intimp.2022.109132
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5408: Show issues Location:
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  9. Article ; Online: Positive RT-PCR Test Results in Patients Recovered From COVID-19.

    Lan, Lan / Xu, Dan / Ye, Guangming / Xia, Chen / Wang, Shaokang / Li, Yirong / Xu, Haibo

    JAMA

    2020  Volume 323, Issue 15, Page(s) 1502–1503

    MeSH term(s) Adult ; Betacoronavirus/genetics ; Betacoronavirus/isolation & purification ; COVID-19 ; COVID-19 Testing ; Clinical Laboratory Techniques ; Coronavirus Infections/diagnosis ; Female ; Humans ; Male ; Pandemics ; Pneumonia, Viral/diagnosis ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2
    Keywords covid19
    Language English
    Publishing date 2020-02-27
    Publishing country United States
    Document type Case Reports ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2958-0
    ISSN 1538-3598 ; 0254-9077 ; 0002-9955 ; 0098-7484
    ISSN (online) 1538-3598
    ISSN 0254-9077 ; 0002-9955 ; 0098-7484
    DOI 10.1001/jama.2020.2783
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  10. Article ; Online: Letter to the Editor: Low-density lipoprotein is a potential predictor of poor prognosis in patients with coronavirus disease 2019.

    Fan, Junli / Wang, Hui / Ye, Guangming / Cao, Xiaoling / Xu, Xianqun / Tan, Wenbin / Zhang, Yongxi

    Metabolism: clinical and experimental

    2020  Volume 107, Page(s) 154243

    Abstract: Background: The pandemic of coronavirus disease 2019 (COVID-19) has become a global threat to public health. The lipid pathophysiology in COVID-19 is unknown.: Methods: In this retrospective longitudinal study, we monitored the serum lipids in 17 ... ...

    Abstract Background: The pandemic of coronavirus disease 2019 (COVID-19) has become a global threat to public health. The lipid pathophysiology in COVID-19 is unknown.
    Methods: In this retrospective longitudinal study, we monitored the serum lipids in 17 surviving and 4 non-surviving COVID-19 cases prior to their viral infections and duration the entire disease courses.
    Results: In surviving cases, the low-density lipoprotein (LDL) levels decreased significantly on admission as compared with the levels before infection; the LDL levels remained constantly low during the disease progression and resumed to the original levels when patients recovered (pre-infection: 3.5 (3.0-4.4); on admission: 2.8 (2.3-3.1), p < 0.01; progression: 2.5 (2.3-3.0); discharge: 3.6 (2.7-4.1); median (IQR), in mmol/L). In non-surviving patients, LDL levels showed an irreversible and continuous decrease until death (1.1 (0.9-1.2), p = 0.02 versus the levels on admission). The ratio changes of LDL levels inversely correlated with ratio changes of high-sensitivity C-reactive protein levels. Logistic regression analysis showed increasing odds of lowered LDL levels associated with disease progression (odds ratio: 4.48, 95% IC: 1.55-12.92, p = 0.006) and in-hospital death (odds ratio: 21.72, 95% IC: 1.40-337.54, p = 0.028).
    Conclusions: LDL levels inversely correlated to disease severities, which could be a predictor for disease progress and poor prognosis.
    MeSH term(s) Aged ; Betacoronavirus ; C-Reactive Protein/analysis ; COVID-19 ; China ; Cholesterol, LDL/blood ; Comorbidity ; Coronavirus Infections/blood ; Coronavirus Infections/diagnosis ; Disease Progression ; Female ; Humans ; Longitudinal Studies ; Male ; Middle Aged ; Pandemics ; Pneumonia, Viral/blood ; Pneumonia, Viral/diagnosis ; Prognosis ; Retrospective Studies ; SARS-CoV-2
    Chemical Substances Cholesterol, LDL ; C-Reactive Protein (9007-41-4)
    Keywords covid19
    Language English
    Publishing date 2020-04-19
    Publishing country United States
    Document type Letter
    ZDB-ID 80230-x
    ISSN 1532-8600 ; 0026-0495
    ISSN (online) 1532-8600
    ISSN 0026-0495
    DOI 10.1016/j.metabol.2020.154243
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