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  1. Article ; Online: Effects of daphnetin on biofilm formation and motility of

    Ye, Zuoji / Ye, Liumei / Li, Dingbin / Lin, Shunsheng / Deng, Wusheng / Zhang, Li / Liang, Jinhua / Li, Jinlong / Wei, Qingjun / Wang, Ke

    Frontiers in cellular and infection microbiology

    2022  Volume 12, Page(s) 1033540

    Abstract: Introduction: Pseudomonas aeruginosa: Methods: In this study, the minimal inhibitory concentration of DAP against : Results: We found that DAP at concentrations of 0.445-1.781 mg/mL and 0.89-1.781 mg/mL can effectively inhibit biofilm formation ... ...

    Abstract Introduction: Pseudomonas aeruginosa
    Methods: In this study, the minimal inhibitory concentration of DAP against
    Results: We found that DAP at concentrations of 0.445-1.781 mg/mL and 0.89-1.781 mg/mL can effectively inhibit biofilm formation and eradicate the formed biofilm of
    Discussion: In conclusion, our results support the conclusion that DAP can effectively eradicate formed biofilm and inhibit biofilm formation, bacterial motility, and pyocyanin production of
    MeSH term(s) Humans ; Pseudomonas aeruginosa ; Pyocyanine ; Umbelliferones/pharmacology ; Anti-Bacterial Agents/pharmacology
    Chemical Substances Pyocyanine (9OQM399341) ; daphnetin (XC84571RD2) ; Umbelliferones ; Anti-Bacterial Agents
    Language English
    Publishing date 2022-11-18
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2022.1033540
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Superoxide dismutase A (SodA) is a c-di-GMP effector protein governing oxidative stress tolerance in Stenotrophomonas maltophilia.

    Sun, Xiao-Yu / Deng, Jie / Zhang, Chenhui / Fung, Sin-Yee / Siu, Kam-Leung / Cheng, Ying-Ying / Ye, Liumei / Qin, Jiaoxia / Wang, Ke / Qu, Jiu-Xin / Gao, Wenying / Wang, Fuxiang / Jin, Dong-Yan / Yang, Liang

    Microbiological research

    2023  Volume 278, Page(s) 127535

    Abstract: C-di-GMP is a bacterial second messenger implicated in the regulation of many key functions including antibiotic tolerance and biofilm formation. Our understanding of how c-di-GMP exerts its action via receptors to modulate different biological functions ...

    Abstract C-di-GMP is a bacterial second messenger implicated in the regulation of many key functions including antibiotic tolerance and biofilm formation. Our understanding of how c-di-GMP exerts its action via receptors to modulate different biological functions is still limited. Here we used a c-di-GMP affinity pull-down assay coupled to LC-MS/MS to identify c-di-GMP-binding proteins in the opportunistic pathogen Stenotrophomonas maltophilia. This analysis identified Smlt3238 (SodA), a protein of the superoxide dismutase family, as a c-di-GMP-binding protein. Microscale thermophoresis showed that purified SodA protein bound c-di-GMP with an estimated dissociation constant (Kd) value of 141.5 μM. Using various in vivo and in vitro experiments, we demonstrated that c-di-GMP modulates the enzyme activity of SodA directly. Circular dichroism experiments revealed that SodA protein gradually altered its basic structure with increasing levels of c-di-GMP. Phenotypic experiments conducted in the presence of a range of intracellular c-di-GMP levels showed that SodA function is modulated by c-di-GMP. The findings thus identify a novel c-di-GMP binding protein that governs oxidative stress tolerance in S. maltophilia.
    MeSH term(s) Stenotrophomonas maltophilia/metabolism ; Bacterial Proteins/metabolism ; Chromatography, Liquid ; Tandem Mass Spectrometry ; Cyclic GMP/metabolism ; Superoxide Dismutase/metabolism ; Carrier Proteins/metabolism ; Oxidative Stress ; Gene Expression Regulation, Bacterial ; Biofilms
    Chemical Substances bis(3',5')-cyclic diguanylic acid (61093-23-0) ; Bacterial Proteins ; Cyclic GMP (H2D2X058MU) ; Superoxide Dismutase (EC 1.15.1.1) ; Carrier Proteins
    Language English
    Publishing date 2023-10-22
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1189614-0
    ISSN 1618-0623 ; 0944-5013
    ISSN (online) 1618-0623
    ISSN 0944-5013
    DOI 10.1016/j.micres.2023.127535
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Ud II Zs.7: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Metagenomic Next-Generation Sequencing Assists in the Diagnosis of Mediastinal

    Deng, Wusheng / Jiang, Yun / Qin, Jiaoxia / Chen, Gang / Lv, Yongjie / Lei, Yanmei / Luo, Jing / Hong, Kangkang / Huang, Bing / Qin, Luhai / Tang, Xiujia / Ye, Liumei / Dang, Yuhai / Wang, Chao / Long, Feiyang / Wang, Ke / Kong, Jinliang

    Infection and drug resistance

    2023  Volume 16, Page(s) 1865–1874

    Abstract: Background: Aspergillus fumigatus: Case presentation: Here, we report a case of a mediastinal : Conclusion: Our study presented a rare case with ... ...

    Abstract Background: Aspergillus fumigatus
    Case presentation: Here, we report a case of a mediastinal
    Conclusion: Our study presented a rare case with mediastinal
    Language English
    Publishing date 2023-03-30
    Publishing country New Zealand
    Document type Case Reports
    ZDB-ID 2494856-1
    ISSN 1178-6973
    ISSN 1178-6973
    DOI 10.2147/IDR.S399484
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: DNase inhibits early biofilm formation in <i>Pseudomonas aeruginosa</i>- or <i>Staphylococcus aureus</i>-induced empyema models.

    Deng, Wusheng / Lei, Yanmei / Tang, Xiujia / Li, Dingbin / Liang, Jinhua / Luo, Jing / Liu, Liuyuan / Zhang, Wenshu / Ye, Liumei / Kong, Jinliang / Wang, Ke / Chen, Zhaoyan

    Frontiers in cellular and infection microbiology

    2022  Volume 12, Page(s) 917038

    Abstract: Anti-infection strategies against pleural empyema include the use of antibiotics and drainage treatments, but bacterial eradication rates remain low. A major challenge is the formation of biofilms in the pleural cavity. DNase has antibiofilm efficacy ... ...

    Abstract Anti-infection strategies against pleural empyema include the use of antibiotics and drainage treatments, but bacterial eradication rates remain low. A major challenge is the formation of biofilms in the pleural cavity. DNase has antibiofilm efficacy in vitro, and intrapleural therapy with DNase is recommended to treat pleural empyema, but the relevant mechanisms remain limited. Our aim was to investigate whether DNase I inhibit the early biofilm formation in Pseudomonas aeruginosa- or Staphylococcus aureus-induced empyema models. We used various assays, such as crystal violet staining, confocal laser scanning microscopy (CLSM) analysis, peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH), and scanning electron microscopy (SEM) analysis. Our results suggested that DNase I significantly inhibited early biofilm formation in a dose-dependent manner, without affecting the growth of P. aeruginosa or S. aureus in vitro. CLSM analysis confirmed that DNase I decreased the biomass and thickness of both bacterial biofilms. The PNA-FISH and SEM analyses also revealed that DNase I inhibited early (24h) biofilm formation in two empyema models. Thus, the results indicated that DNase inhibited early (24h) biofilm formation in P. aeruginosa- or S. aureus-induced rabbit empyema models and showed its therapeutic potential against empyema biofilms.
    MeSH term(s) Animals ; Rabbits ; Pseudomonas aeruginosa ; Staphylococcus aureus ; Deoxyribonucleases/pharmacology ; In Situ Hybridization, Fluorescence ; Staphylococcal Infections/drug therapy ; Biofilms ; Anti-Bacterial Agents/therapeutic use ; Deoxyribonuclease I/pharmacology ; Empyema, Pleural
    Chemical Substances Deoxyribonucleases (EC 3.1.-) ; Anti-Bacterial Agents ; Deoxyribonuclease I (EC 3.1.21.1)
    Language English
    Publishing date 2022-10-12
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2022.917038
    Database MEDical Literature Analysis and Retrieval System OnLINE

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