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Article: Simultaneous quantification of six indicator compounds in Wen-Qing-Yin by high-performance liquid chromatography-diode array detection

Yeh, Chi-Chen / Huang, Shih-Shan / Liu, Po-Yu / Wang, Bo-Cheng / Tsai, Chia-Fen / Wang, Der-Yuan / Cheng, Hwei-Fang

Yàowù shípǐn fēnxī. 2019 July, v. 27, no. 3

2019

Abstract: A simple gradient high-performance liquid chromatography with diode array detection (HPLC-DAD) method was used to simultaneously to analyze characteristics of six indicator compounds in the traditional Chinese medicine (TCM) formulation Wen-Qing-Yin (WQY) ...

Abstract	A simple gradient high-performance liquid chromatography with diode array detection (HPLC-DAD) method was used to simultaneously to analyze characteristics of six indicator compounds in the traditional Chinese medicine (TCM) formulation Wen-Qing-Yin (WQY). Separate optimization was performed using a Cosmosil C18 column gradient method with 0.1% formic acid in both mobile phases of aqueous and acetonitrile (ACN), at a flow rate, detection wavelength, and sample volume of 1.8 mL/min, 268 nm, and 10 μL, respectively. The linear regression of six active compounds berberine (BER), baicalin (BAI), ferulic acid (FER), geniposide (GEN), hydorxymethoxylfurfural (HMF), and paeoniflorin (PAE) was produced at the concentration range of 10–2000 μg/mL. The method validation revealed an acceptable precision (intra- and inter-day precision < 3.39% and 4.11%, respectively) and recovery (85.60–110.45% and 86.58–110.90%), a recovery range of 86.61–109.42%, and sensitivity (limit of detection [LOD] and limit of quantification [LOQ] values were in the range of 0.03–3.13, and 0.08–9.38 μg/mL, respectively) while the calibration curves were linear with a correlation coefficient (R2) ranging from 0.9966 to 0.9989. The qualitative and quantitative analyses were performed by direct comparison of the peaks of the WCY extract to retention times of reference standards. Additionally, principal component analysis (PCA) successfully discriminated four purchased commercial samples of all six indicator constituents, and the present results indicate their comprehensive potential usefulness for qualitative and quantitative analyses of the WQY decoction and its commercial products.
Keywords	Oriental traditional medicine ; acetonitrile ; active ingredients ; baicalin ; berberine ; calibration ; detection limit ; diodes ; ferulic acid ; formic acid ; high performance liquid chromatography ; principal component analysis ; quantitative analysis ; reference standards ; regression analysis ; wavelengths
Language	English
Dates of publication	2019-07
Size	p. 749-757.
Publishing place	Elsevier Taiwan LLC.
Document type	Article
ZDB-ID	1292535-4
ISSN	1021-9498
ISSN	1021-9498
DOI	10.1016/j.jfda.2018.12.003
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Simultaneous quantification of six indicator compounds in Wen-Qing-Yin by high-performance liquid chromatography-diode array detection.

Yeh, Chi-Chen / Huang, Shih-Shan / Liu, Po-Yu / Wang, Bo-Cheng / Tsai, Chia-Fen / Wang, Der-Yuan / Cheng, Hwei-Fang

Journal of food and drug analysis

2018 Volume 27, Issue 3, Page(s) 749–757

Abstract	A simple gradient high-performance liquid chromatography with diode array detection (HPLC-DAD) method was used to simultaneously to analyze characteristics of six indicator compounds in the traditional Chinese medicine (TCM) formulation Wen-Qing-Yin (WQY). Separate optimization was performed using a Cosmosil C18 column gradient method with 0.1% formic acid in both mobile phases of aqueous and acetonitrile (ACN), at a flow rate, detection wavelength, and sample volume of 1.8 mL/min, 268 nm, and 10 μL, respectively. The linear regression of six active compounds berberine (BER), baicalin (BAI), ferulic acid (FER), geniposide (GEN), hydorxymethoxylfurfural (HMF), and paeoniflorin (PAE) was produced at the concentration range of 10-2000 μg/mL. The method validation revealed an acceptable precision (intra- and inter-day precision < 3.39% and 4.11%, respectively) and recovery (85.60-110.45% and 86.58-110.90%), a recovery range of 86.61-109.42%, and sensitivity (limit of detection [LOD] and limit of quantification [LOQ] values were in the range of 0.03-3.13, and 0.08-9.38 μg/mL, respectively) while the calibration curves were linear with a correlation coefficient (R
MeSH term(s)	Berberine/analysis ; Chromatography, High Pressure Liquid ; Coumaric Acids/analysis ; Drugs, Chinese Herbal/chemistry ; Flavonoids/analysis ; Furaldehyde/analysis ; Glucosides/analysis ; Iridoids/analysis ; Medicine, Chinese Traditional ; Monoterpenes/analysis
Chemical Substances	Coumaric Acids ; Drugs, Chinese Herbal ; Flavonoids ; Glucosides ; Iridoids ; Monoterpenes ; Berberine (0I8Y3P32UF) ; geniposide (145295QLXY) ; peoniflorin (21AIQ4EV64) ; baicalin (347Q89U4M5) ; ferulic acid (AVM951ZWST) ; Furaldehyde (DJ1HGI319P)
Language	English
Publishing date	2018-12-28
Publishing country	China (Republic : 1949- )
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2224-6614
ISSN (online)	2224-6614
DOI	10.1016/j.jfda.2018.12.003
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Marine algal natural products with anti-oxidative, anti-inflammatory, and anti-cancer properties.

Lee, Jin-Ching / Hou, Ming-Feng / Huang, Hurng-Wern / Chang, Fang-Rong / Yeh, Chi-Chen / Tang, Jen-Yang / Chang, Hsueh-Wei

Cancer cell international

2013 Volume 13, Issue 1, Page(s) 55

Abstract: For their various bioactivities, biomaterials derived from marine algae are important ingredients in many products, such as cosmetics and drugs for treating cancer and other diseases. This mini-review comprehensively compares the bioactivities and ... ...

Abstract	For their various bioactivities, biomaterials derived from marine algae are important ingredients in many products, such as cosmetics and drugs for treating cancer and other diseases. This mini-review comprehensively compares the bioactivities and biological functions of biomaterials from red, green, brown, and blue-green algae. The anti-oxidative effects and bioactivities of several different crude extracts of algae have been evaluated both in vitro and in vivo. Natural products derived from marine algae protect cells by modulating the effects of oxidative stress. Because oxidative stress plays important roles in inflammatory reactions and in carcinogenesis, marine algal natural products have potential for use in anti-cancer and anti-inflammatory drugs.
Language	English
Publishing date	2013-06-03
Publishing country	England
Document type	Journal Article
ISSN	1475-2867
ISSN	1475-2867
DOI	10.1186/1475-2867-13-55
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Article ; Online: Butanol-Partitioned Extraction from Aqueous Extract of Gracilaria tenuistipitata Inhibits Cell Proliferation of Oral Cancer Cells Involving Apoptosis and Oxidative Stress.

Yeh, Chi-Chen / Li, Kun-Tzu / Tang, Jen-Yang / Wang, Hui-Ru / Liu, Jing-Ru / Huang, Hurng-Wern / Chang, Fang-Rong / Tsai, Cheng-En / Lo, I-Wen / Huang, Ming-Yii / Chang, Hsueh-Wei

DNA and cell biology

2016 Volume 35, Issue 5, Page(s) 210–216

Abstract: We have previously found that the aqueous extract of Gracilaria tenuistipitata (AEGT) and its partitioned fractions had antioxidant properties in biochemical assays. Although the butanol-partitioned fraction of AEGT (AEGT-pBuOH) had a stronger ... ...

Abstract	We have previously found that the aqueous extract of Gracilaria tenuistipitata (AEGT) and its partitioned fractions had antioxidant properties in biochemical assays. Although the butanol-partitioned fraction of AEGT (AEGT-pBuOH) had a stronger antioxidant performance than AEGT, its biological effects are still unknown. In this study, the cellular responses of oral cancer cells to AEGT-pBuOH were monitored in terms of cell viability, cell cycle progression, apoptosis, and oxidative stress responses. In an ATP content assay, the cell viability of oral cancer cells treated with AEGT-pBuOH was dose responsively inhibited (p < 0.005). For flow cytometry, AEGT-pBuOH was also found to dose responsively induce cell cycle disturbance by propidium iodide (PI) staining and to induce apoptosis by annexin V/PI and pan-caspase staining (p < 0.005). In AEGT-pBuOH-treated oral cancer cells, the reactive oxygen species (ROS) was increased and mitochondrial membrane potential was decreased in a dose-response manner (p < 0.005). These results suggest that AEGT-pBuOH inhibited the proliferation and induced apoptosis of oral cancer cells involving the ROS generation and mitochondrial depolarization.
MeSH term(s)	Antineoplastic Agents, Phytogenic/pharmacology ; Antioxidants/pharmacology ; Apoptosis/drug effects ; Cell Line, Tumor ; Cell Proliferation/drug effects ; DNA Damage/drug effects ; Gracilaria/chemistry ; Humans ; Membrane Potential, Mitochondrial/drug effects ; Mouth Neoplasms/pathology ; Oxidative Stress/drug effects ; Plant Extracts/pharmacology ; Reactive Oxygen Species/metabolism
Chemical Substances	Antineoplastic Agents, Phytogenic ; Antioxidants ; Plant Extracts ; Reactive Oxygen Species
Language	English
Publishing date	2016-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	1024454-2
ISSN	1557-7430 ; 0198-0238 ; 1044-5498
ISSN (online)	1557-7430
ISSN	0198-0238 ; 1044-5498
DOI	10.1089/dna.2015.3044
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Article ; Online: Antiproliferation and induction of apoptosis in Ca9-22 oral cancer cells by ethanolic extract of Gracilaria tenuistipitata.

Yeh, Chi-Chen / Tseng, Chao-Neng / Yang, Jing-Iong / Huang, Hurng-Wern / Fang, Yi / Tang, Jen-Yang / Chang, Fang-Rong / Chang, Hsueh-Wei

Molecules (Basel, Switzerland)

2012 Volume 17, Issue 9, Page(s) 10916–10927

Abstract: The water extract of Gracilaria tenuistipitata have been found to be protective against oxidative stress-induced cellular DNA damage, but the biological function of the ethanolic extracts of G. tenuistipitata (EEGT) is still unknown. In this study, the ... ...

Abstract	The water extract of Gracilaria tenuistipitata have been found to be protective against oxidative stress-induced cellular DNA damage, but the biological function of the ethanolic extracts of G. tenuistipitata (EEGT) is still unknown. In this study, the effect of EEGT on oral squamous cell cancer (OSCC) Ca9-22 cell line was examined in terms of the cell proliferation and oxidative stress responses. The cell viability of EEGT-treated OSCC cells was significantly reduced in a dose-response manner (p < 0.0001). The annexin V intensity and pan-caspase activity of EEGT-treated OSCC cells were significantly increased in a dose-response manner (p < 0.05 to 0.0001). EEGT significantly increased the reactive oxygen species (ROS) level (p < 0.0001) and decreased the glutathione (GSH) level (p < 0.01) in a dose-response manner. The mitochondrial membrane potential (MMP) of EEGT-treated OSCC cells was significantly decreased in a dose-response manner (p < 0.005). In conclusion, we have demonstrated that EEGT induced the growth inhibition and apoptosis of OSCC cells, which was accompanied by ROS increase, GSH depletion, caspase activation, and mitochondrial depolarization. Therefore, EEGT may have potent antitumor effect against oral cancer cells.
MeSH term(s)	Antineoplastic Agents, Phytogenic/pharmacology ; Apoptosis/drug effects ; Caspases/metabolism ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Ethanol ; Glutathione/analysis ; Gracilaria ; Humans ; Membrane Potential, Mitochondrial/drug effects ; Mitochondria/drug effects ; Mouth Neoplasms/drug therapy ; Oxidative Stress/drug effects ; Plant Extracts/pharmacology ; Reactive Oxygen Species/metabolism
Chemical Substances	Antineoplastic Agents, Phytogenic ; Plant Extracts ; Reactive Oxygen Species ; Ethanol (3K9958V90M) ; Caspases (EC 3.4.22.-) ; Glutathione (GAN16C9B8O)
Language	English
Publishing date	2012-09-11
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules170910916
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Article ; Online: Aqueous extracts of the edible Gracilaria tenuistipitata are protective against H₂O₂-induced DNA damage, growth inhibition, and cell cycle arrest.

Yang, Jing-Iong / Yeh, Chi-Chen / Lee, Jin-Ching / Yi, Szu-Cheng / Huang, Hurng-Wern / Tseng, Chao-Neng / Chang, Hsueh-Wei

Molecules (Basel, Switzerland)

2012 Volume 17, Issue 6, Page(s) 7241–7254

Abstract: Potential antioxidant properties of an aqueous extract of the edible red seaweed Gracilaria tenuistipitata (AEGT) against oxidative DNA damage were evaluated. The AEGT revealed several antioxidant molecules, including phenolics, flavonoids and ascorbic ... ...

Abstract	Potential antioxidant properties of an aqueous extract of the edible red seaweed Gracilaria tenuistipitata (AEGT) against oxidative DNA damage were evaluated. The AEGT revealed several antioxidant molecules, including phenolics, flavonoids and ascorbic acid. In a cell-free assay, the extract exhibited 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity that significantly reduced H₂O₂-induced plasmid DNA breaks in a dose-response manner (P < 0.001). The AEGT also suppressed H₂O₂-induced oxidative DNA damage in H1299 cells by reducing the percentage of damaged DNA in a dose-response manner (P < 0.001) as measured by a modified alkaline comet-nuclear extract (comet-NE) assay. The MTT assay results showed that AEGT confers significant protection against H₂O₂-induced cytotoxicity and that AEGT itself is not cytotoxic (P < 0.001). Moreover, H₂O₂-induced cell cycle G2/M arrest was significantly released when cells were co-treated with different concentrations of AEGT (P < 0.001). Taken together, these findings suggest that edible red algae Gracilaria water extract can prevent H₂O₂-induced oxidative DNA damage and its related cellular responses.
MeSH term(s)	Antioxidants/chemistry ; Antioxidants/pharmacology ; Ascorbic Acid/chemistry ; Cell Cycle Checkpoints/drug effects ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; DNA Breaks, Double-Stranded/drug effects ; DNA Damage/drug effects ; Flavonoids/chemistry ; Free Radical Scavengers/chemistry ; Free Radical Scavengers/pharmacology ; Gracilaria/chemistry ; Humans ; Hydrogen Peroxide/pharmacology ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Plasmids ; Polyphenols/chemistry
Chemical Substances	Antioxidants ; Flavonoids ; Free Radical Scavengers ; Plant Extracts ; Polyphenols ; Hydrogen Peroxide (BBX060AN9V) ; Ascorbic Acid (PQ6CK8PD0R)
Language	English
Publishing date	2012-06-13
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules17067241
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Article ; Online: Aqueous extract of Gracilaria tenuistipitata suppresses LPS-induced NF-κB and MAPK activation in RAW 264.7 and rat peritoneal macrophages and exerts hepatoprotective effects on carbon tetrachloride-treated rat.

Tseng, Chin-Kai / Lin, Chun-Kuang / Chang, Hsueh-Wei / Wu, Yu-Hsuan / Yen, Feng-Lin / Chang, Fang-Rong / Chen, Wei-Chun / Yeh, Chi-Chen / Lee, Jin-Ching

PloS one

2014 Volume 9, Issue 1, Page(s) e86557

Abstract: In addition to the previous investigations of bioactivity of aqueous extract of the edible Gracilaria tenuistipitata (AEGT) against H2O2-induced DNA damage and hepatitis C virus replication, the purpose of this study is to evaluate the potential ... ...

Abstract	In addition to the previous investigations of bioactivity of aqueous extract of the edible Gracilaria tenuistipitata (AEGT) against H2O2-induced DNA damage and hepatitis C virus replication, the purpose of this study is to evaluate the potential therapeutic properties of AEGT against inflammation and hepatotoxicity using lipopolysaccharide (LPS)-stimulated mouse RAW 264.7 cells, primary rat peritoneal macrophages and carbon tetrachloride (CCl4)-induced acute hepatitis model in rats. AEGT concentration-dependently inhibited the elevated RNA and protein levels of inducible nitric oxide synthase and cyclooxygenase-2, thereby reducing nitric oxide and prostaglandin E2 levels, respectively. Moreover, AEGT significantly suppressed the production of LPS-induced proinflammatory cytokines, including interleukin (IL)-1β, IL-6 and tumor necrosis factor-α. These inhibitory effects were associated with the suppression of nuclear factor-kappa B activation and mitogen-activated protein kinase phosphorylation by AEGT in LPS-stimulated cells. In addition, we highlighted the hepatoprotective and curative effects of AEGT in a rat model of CCl4-intoxicated acute liver injury, which was evident from reduction in the elevated serum aspartate aminotransferase and alanine aminotransferase levels as well as amelioration of histological damage by pre-treatment or post-treatment of AEGT. In conclusion, the results demonstrate that AEGT may serve as a potential supplement in the prevention or amelioration of inflammatory diseases.
MeSH term(s)	Animals ; Blotting, Western ; Carbon Tetrachloride/adverse effects ; Cell Line ; Chemical and Drug Induced Liver Injury/drug therapy ; Enzyme Activation/drug effects ; Gracilaria/chemistry ; Macrophages/metabolism ; Magnetic Resonance Spectroscopy ; Mice ; Mitogen-Activated Protein Kinases/metabolism ; NF-kappa B/metabolism ; Oligonucleotides/genetics ; Phytotherapy/methods ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Rats
Chemical Substances	NF-kappa B ; Oligonucleotides ; Plant Extracts ; Carbon Tetrachloride (CL2T97X0V0) ; Mitogen-Activated Protein Kinases (EC 2.7.11.24)
Language	English
Publishing date	2014-01-27
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0086557
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Aqueous extract of the edible Gracilaria tenuistipitata inhibits hepatitis C viral replication via cyclooxygenase-2 suppression and reduces virus-induced inflammation.

Chen, Kuan-Jen / Tseng, Chin-Kai / Chang, Fang-Rong / Yang, Jin-Iong / Yeh, Chi-Chen / Chen, Wei-Chun / Wu, Shou-Fang / Chang, Hsueh-Wei / Lee, Jin-Ching

PloS one

2013 Volume 8, Issue 2, Page(s) e57704

Abstract: Hepatitis C virus (HCV) is an important human pathogen leading to hepatocellular carcinoma. Using an in vitro cell-based HCV replicon and JFH-1 infection system, we demonstrated that an aqueous extract of the seaweed Gracilaria tenuistipitata (AEGT) ... ...

Abstract	Hepatitis C virus (HCV) is an important human pathogen leading to hepatocellular carcinoma. Using an in vitro cell-based HCV replicon and JFH-1 infection system, we demonstrated that an aqueous extract of the seaweed Gracilaria tenuistipitata (AEGT) concentration-dependently inhibited HCV replication at nontoxic concentrations. AEGT synergistically enhanced interferon-α (IFN-α) anti-HCV activity in a combination treatment. We found that AEGT also significantly suppressed virus-induced cyclooxygenase-2 (COX-2) expression at promoter transactivation and protein levels. Notably, addition of exogenous COX-2 expression in AEGT-treated HCV replicon cells gradually abolished AEGT anti-HCV activity, suggesting that COX-2 down-regulation was responsible for AEGT antiviral effects. Furthermore, we highlighted the inhibitory effect of AEGT in HCV-induced pro-inflammatory gene expression such as the expression of tumour necrosis factor-α, interleukin-1β, inducible nitrite oxide synthase and COX-2 in a concentration-dependent manner to evaluate the potential therapeutic supplement in the management of patients with chronic HCV infections.
MeSH term(s)	Antiviral Agents/pharmacology ; Antiviral Agents/therapeutic use ; Cell Line, Tumor ; Cyclooxygenase 2/genetics ; Cyclooxygenase 2/metabolism ; Down-Regulation/drug effects ; Drug Synergism ; Gracilaria/chemistry ; Hepacivirus/drug effects ; Hepacivirus/metabolism ; Hepacivirus/physiology ; Humans ; Inflammation/drug therapy ; Inflammation/genetics ; Inflammation/metabolism ; Inflammation/virology ; Interferon-alpha/pharmacology ; Interleukin-1beta/genetics ; NF-kappa B/metabolism ; Nitric Oxide Synthase Type II/genetics ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Plants, Edible/chemistry ; RNA, Viral/biosynthesis ; Tumor Necrosis Factor-alpha/genetics ; Viral Nonstructural Proteins/biosynthesis ; Viral Nonstructural Proteins/metabolism ; Viral Proteins/biosynthesis ; Virus Replication/drug effects ; Water/chemistry
Chemical Substances	Antiviral Agents ; Interferon-alpha ; Interleukin-1beta ; NF-kappa B ; Plant Extracts ; RNA, Viral ; Tumor Necrosis Factor-alpha ; Viral Nonstructural Proteins ; Viral Proteins ; Water (059QF0KO0R) ; Nitric Oxide Synthase Type II (EC 1.14.13.39) ; Cyclooxygenase 2 (EC 1.14.99.1) ; NS-5 protein, hepatitis C virus (EC 2.7.7.48)
Language	English
Publishing date	2013-02-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0057704
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Anti-proliferative effect of methanolic extract of Gracilaria tenuistipitata on oral cancer cells involves apoptosis, DNA damage, and oxidative stress.

Yeh, Chi-Chen / Yang, Jing-Iong / Lee, Jin-Ching / Tseng, Chao-Neng / Chan, Ya-Ching / Hseu, You-Cheng / Tang, Jen-Yang / Chuang, Li-Yeh / Huang, Hurng-Wern / Chang, Fang-Rong / Chang, Hsueh-Wei

BMC complementary and alternative medicine

2012 Volume 12, Page(s) 142

Abstract: Background: Methanolic extracts of Gracilaria tenuistipitata (MEGT) were obtained from the edible red algae. Previously, we found that water extract of G. tenuistipitata was able to modulate oxidative stress-induced DNA damage and its related cellular ... ...

Abstract	Background: Methanolic extracts of Gracilaria tenuistipitata (MEGT) were obtained from the edible red algae. Previously, we found that water extract of G. tenuistipitata was able to modulate oxidative stress-induced DNA damage and its related cellular responses. Methods: In this study, the methanol extraction product MEGT was used to evaluate the cell growth inhibition in oral cancer cells and its possible mechanism was investigated. Results: The cell viability of MEGT treated Ca9-22 oral cancer cell line was significantly decreased in a dose-response manner (p < 0.05). The sub-G1 population and annexin V intensity of MEGT-treated Ca9-22 cancer cells were significantly increased in a dose-response manner (p < 0.0005 and p < 0.001, respectively). The γH2AX intensities of MEGT-treated Ca9-22 cancer cells were significantly increased in a dose-response manner (p < 0.05). The reactive oxygen species (ROS) and glutathione (GSH)-positive intensities of MEGT-treated Ca9-22 oral cancer cells were significantly increased and decreased, respectively, in a dose-response manner (p < 0.05). The DiOC2(3) intensity for mitochondrial membrane potential (MMP) of MEGT-treated Ca9-22 cancer cells was significantly decreased in a dose-response manner (p < 0.05). Conclusions: These results indicated that MEGT had apoptosis-based cytotoxicity against oral cancer cells through the DNA damage, ROS induction, and mitochondrial depolarization. Therefore, MEGT derived from the edible algae may have potential therapeutic effects against oral squamous cell carcinoma (OSCC).
MeSH term(s)	Apoptosis/drug effects ; Cell Line, Tumor ; Cell Proliferation/drug effects ; DNA Damage/drug effects ; Gracilaria/chemistry ; Growth Inhibitors/pharmacology ; Humans ; Mouth Neoplasms/drug therapy ; Mouth Neoplasms/genetics ; Mouth Neoplasms/metabolism ; Mouth Neoplasms/physiopathology ; Oxidative Stress/drug effects ; Plant Extracts/pharmacology
Chemical Substances	Growth Inhibitors ; Plant Extracts
Language	English
Publishing date	2012-08-31
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2050429-9
ISSN	1472-6882 ; 1472-6882
ISSN (online)	1472-6882
ISSN	1472-6882
DOI	10.1186/1472-6882-12-142
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Antiproliferative effects of goniothalamin on Ca9-22 oral cancer cells through apoptosis, DNA damage and ROS induction.

Yen, Ching-Yu / Chiu, Chien-Chih / Haung, Rou-Wen / Yeh, Chi-Chen / Huang, Kuang-Jing / Chang, Kuo-Feng / Hseu, You-Cheng / Chang, Fang-Rong / Chang, Hsueh-Wei / Wu, Yang-Chang

Mutation research

2012 Volume 747, Issue 2, Page(s) 253–258

Abstract: Goniothalamin (GTN), a plant bioactive styryl-lactone, is a natural product with potent anti-tumorigenesis effects for several types of cancer. Nonetheless, the anticancer effect of GTN has not been examined in oral cancer. The present study was designed ...

Abstract	Goniothalamin (GTN), a plant bioactive styryl-lactone, is a natural product with potent anti-tumorigenesis effects for several types of cancer. Nonetheless, the anticancer effect of GTN has not been examined in oral cancer. The present study was designed to evaluate its potential anticancer effects in an oral squamous cell carcinoma (OSCC) model and to determine the possible mechanisms with respect to apoptosis, DNA damage, reactive oxygen species (ROS) induction, and mitochondrial membrane potential. Our data demonstrated that cell proliferation was significantly inhibited by GTN in Ca9-22 OSCC cancer cells in concentration- and time-dependent manners (p<0.05). For cell cycle and apoptotic effects of GTN-treated Ca9-22 cancer cells, the sub-G1 population and annexin V-intensity significantly increased in a concentration-dependent manner (p<0.001). For the analysis of DNA double strand breaks, γH2AX intensity significantly increased in GTN-treated Ca9-22 cancer cells in concentration-response relationship (p<0.05). Moreover, GTN significantly induced intracellular ROS levels in Ca9-22 cancer cells in a concentration- and time-dependent manner (p<0.05). For membrane depolarization of mitochondria, the DiOC(2)(3) (3,3'-diethyloxacarbocyanine iodide) intensity of GTN-treated Ca9-22 cancer cells was significantly decreased in concentration- and time-dependent relationships (p<0.001). Taken together, these results suggest that the anticancer effect of GTN against oral cancer cells is valid and GTN-induced growth inhibition and apoptosis influence the downstream cascade including ROS induction, DNA damage, and mitochondria membrane depolarization. Therefore, GTN has potential as a chemotherapeutic agent against oral cancer.
MeSH term(s)	Antineoplastic Agents, Phytogenic/pharmacology ; Apoptosis/drug effects ; Carcinoma, Squamous Cell/drug therapy ; Cell Line, Tumor ; Cell Proliferation/drug effects ; DNA Breaks, Double-Stranded/drug effects ; DNA Damage/drug effects ; Dose-Response Relationship, Drug ; Humans ; Membrane Potential, Mitochondrial/drug effects ; Mouth Neoplasms/drug therapy ; Pyrones/pharmacology ; Reactive Oxygen Species/metabolism
Chemical Substances	Antineoplastic Agents, Phytogenic ; Pyrones ; Reactive Oxygen Species ; goniothalamin (34W9GO6B2Z)
Language	English
Publishing date	2012-09-18
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	206607-5
ISSN	1873-135X ; 0027-5107 ; 1383-5718 ; 0165-1110 ; 0165-1161 ; 0165-7992 ; 0921-8777 ; 0165-1218 ; 1383-5726 ; 0167-8817 ; 0921-8734 ; 1383-5742
ISSN (online)	1873-135X
ISSN	0027-5107 ; 1383-5718 ; 0165-1110 ; 0165-1161 ; 0165-7992 ; 0921-8777 ; 0165-1218 ; 1383-5726 ; 0167-8817 ; 0921-8734 ; 1383-5742
DOI	10.1016/j.mrgentox.2012.06.003
Database	MEDical Literature Analysis and Retrieval System OnLINE

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