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Article ; Online: Supramolecular fluorescence sensor array used for the analysis of tyrosine kinase inhibitors in biological fluids and cell imaging.

Yi, Hong-Ling / Yang, Ru-Pei / Tang, Qing / Tao, Zhu / Huang, Ying

2023 Volume 1287, Page(s) 342124

Abstract: Tyrosine kinase inhibitors (TKIs) are commonly used in tumor targeting therapy. However, the rapid analysis of TKIs remains a significant challenge, especially in complex biological fluid environments. In this work, we have constructed a supramolecular ... ...

Abstract	Tyrosine kinase inhibitors (TKIs) are commonly used in tumor targeting therapy. However, the rapid analysis of TKIs remains a significant challenge, especially in complex biological fluid environments. In this work, we have constructed a supramolecular fluorescence sensor array based on a cucurbituril-dye host-guest complex. The binding affinity between the three complexes and each TKI is different, resulting in different cross-response signals of the complexes to the fluorescence of each TKI. Combined with linear discriminant analysis(LDA), five kinds of TKIs can be well identified. The supramolecular fluorescence sensor array could accurately identify and distinguish the five TKIs in water and could classify mixtures containing different concentrations of TKIs in serum. The concentration and Factor 1 exhibited a good linear relationship and the detection limit (LOD) was as low as 10
MeSH term(s)	Reproducibility of Results ; Tyrosine Kinase Inhibitors ; Cell Differentiation ; Discriminant Analysis ; Drug Monitoring
Chemical Substances	Tyrosine Kinase Inhibitors
Language	English
Publishing date	2023-12-09
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1483436-4
ISSN	1873-4324 ; 0003-2670
ISSN (online)	1873-4324
ISSN	0003-2670
DOI	10.1016/j.aca.2023.342124
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Article ; Online: A white-light-emitting sensor array based on cucurbit[8]uril for quantitative detection of multicomponent nitroaniline isomers.

Huang, Shu-Zhen / Wei, Kai-Ni / Yang, Ru-Pei / Yi, Hong-Ling / Tang, Qing / Tao, Zhu / Huang, Ying

Mikrochimica acta

2023 Volume 190, Issue 12, Page(s) 460

Abstract: A white-light-emitting supramolecular complex through supramolecular interactions has been assembled; the white luminescent supramolecular complex exhibits two emission spectra. Based on this, a dual-channel white-light array sensor was constructed. The ... ...

Abstract	A white-light-emitting supramolecular complex through supramolecular interactions has been assembled; the white luminescent supramolecular complex exhibits two emission spectra. Based on this, a dual-channel white-light array sensor was constructed. The results show that it can quickly identify and detect nitroaniline isomer pollutants (p-nitroaniline, m-nitroaniline, o-nitroaniline). When these three nitroaniline isomers were added to the supramolecular white-light array sensor, the fluorescence intensity of the white-light complex decreased to varying degrees. Linear discriminant analysis (LDA) showed that the supramolecular white-light array sensor could recognize and distinguish three nitroaniline isomers and could classify mixtures containing different concentrations. Factor 1 of the array had a good linear relationship with the concentration of pollutants, and the detection limit (LOD) was as low as 0.7 μM. The method has good reproducibility and stability. In addition, it can also qualitatively detect the nitroaniline isomers in river water and contaminated rice seedling extract. It provides an ideal platform for constructing multiresponse sensors.
Language	English
Publishing date	2023-11-04
Publishing country	Austria
Document type	Journal Article
ZDB-ID	89-9
ISSN	1436-5073 ; 0026-3672
ISSN (online)	1436-5073
ISSN	0026-3672
DOI	10.1007/s00604-023-06040-2
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Article ; Online: Integrated genetic and epigenetic analysis reveals DNA repair alterations in multifocal hepatocellular carcinoma

Yi-Hong Ling / Meng-Ni Liu / Yi-Xin Yin / Zhong-Guo Zhou / Jie-Wei Chen / Wei Wei / Jing-Ping Yun / Dan Xie / Rong-Ping Guo / Mu-Yan Cai

Signal Transduction and Targeted Therapy, Vol 8, Iss 1, Pp 1-

2023 Volume 4

Keywords	Medicine ; R ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	Nature Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Clinical actionability of triaging DNA mismatch repair deficient colorectal cancer from biopsy samples using deep learning

Wu Jiang / Wei-Jian Mei / Shuo-Yu Xu / Yi-Hong Ling / Wei-Rong Li / Jin-Bo Kuang / Hao-Sen Li / Hui Hui / Ji-Bin Li / Mu-Yan Cai / Zhi-Zhong Pan / Hui-Zhong Zhang / Li Li / Pei-Rong Ding

EBioMedicine, Vol 81, Iss , Pp 104120- (2022)

2022

Abstract: Summary: Background: We aimed to develop a deep learning (DL) model to predict DNA mismatch repair (MMR) status in colorectal cancers (CRC) based on hematoxylin and eosin-stained whole-slide images (WSIs) and assess its clinical applicability. Methods: ... ...

Abstract	Summary: Background: We aimed to develop a deep learning (DL) model to predict DNA mismatch repair (MMR) status in colorectal cancers (CRC) based on hematoxylin and eosin-stained whole-slide images (WSIs) and assess its clinical applicability. Methods: The DL model was developed and validated through three-fold cross validation using 441 WSIs from the Cancer Genome Atlas (TCGA) and externally validated using 78 WSIs from the Pathology AI Platform (PAIP), and 355 WSIs from surgical specimens and 341 WSIs from biopsy specimens of the Sun Yet-sun University Cancer Center (SYSUCC). Domain adaption and multiple instance learning (MIL) techniques were adopted for model development. The performance of the models was evaluated using the area under the receiver operating characteristic curve (AUROC). A dual-threshold strategy was also built from the surgical cohorts and validated in the biopsy cohort. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), F1-score, and the percentage of patients avoiding IHC testing were evaluated. Findings: The MIL model achieved an AUROC of 0·8888±0·0357 in the TCGA-validation cohort, 0·8806±0·0232 in the PAIP cohort, 0·8457±0·0233 in the SYSUCC-surgical cohort, and 0·7679±0·0342 in the SYSUCC-biopsy cohort. A dual-threshold triage strategy was used to rule-in and rule-out dMMR patients with remaining uncertain patients recommended for further IHC testing, which kept sensitivity higher than 90% and specificity higher than 95% on deficient MMR patient triage from both the surgical and biopsy specimens, result in more than half of patients avoiding IHC based MMR testing. Interpretation: A DL-based method that could directly predict CRC MMR status from WSIs was successfully developed, and a dual-threshold triage strategy was established to minimize the number of patients for further IHC testing. Funding: The study was funded by the National Natural Science Foundation of China (82073159, 81871971 and 81700576), the Natural Science Foundation of ...
Keywords	Colorectal cancer ; Mismatch repair-deficient ; Deep learning ; Screening strategy ; Dual-threshold ; Medicine ; R ; Medicine (General) ; R5-920
Subject code	616
Language	English
Publishing date	2022-07-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: [Clinical value of fluorescence lateral flow immunoassay in diagnosis of influenza A in children].

Guo, Chun / Zhong, Li-Li / Yi, Hong-Ling / Chen, Min

Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics

2016 Volume 18, Issue 12, Page(s) 1272–1276

Abstract: Objective: To evaluate the clinical value of a new type of fluorescence lateral flow immunoassay in rapid detection of influenza A virus.: Methods: A total of 378 samples of nasopharyngeal secretions were collected from 378 children with influenza- ... ...

Abstract	Objective: To evaluate the clinical value of a new type of fluorescence lateral flow immunoassay in rapid detection of influenza A virus. Methods: A total of 378 samples of nasopharyngeal secretions were collected from 378 children with influenza-like symptoms to detect the influenza A virus by fluorescence lateral flow immunoassay, colloidal gold immunoassay, and RT-PCR between July 2015 and August 2015. Results: Of the 378 samples, 81 (21.4%) were positive for influenza A virus by RT-PCR. Compared with RT-PCR, the sensitivities of fluorescence lateral flow immunoassay and colloidal gold immunoassay were 90.1% (73/81) and 75.3% (61/81), respectively, and the specificities were 99.3% (295/297) and 98.3% (292/297), respectively. The average threshold cycle (Ct) value for the positive samples detected by the fluorescence lateral flow immunoassay (30.6) was higher than that for the positive samples detected by the colloidal gold immunoassay (28.7). Conclusions: Compared with colloidal gold immunoassay, fluorescence lateral flow immunoassay has higher sensitivity, specificity, and concordance rate with RT-PCR, suggesting that it can be used for early screening and diagnosis of influenza A.
MeSH term(s)	Child ; Child, Preschool ; Female ; Fluorescence ; Humans ; Immunoassay/methods ; Infant ; Influenza A virus/isolation & purification ; Influenza, Human/diagnosis ; Male ; Real-Time Polymerase Chain Reaction
Language	Chinese
Publishing date	2016-12-09
Publishing country	China
Document type	Journal Article
ISSN	1008-8830
ISSN	1008-8830
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: SATB2 is a Promising Biomarker for Identifying a Colorectal Origin for Liver Metastatic Adenocarcinomas

Yi-Jun Zhang / Jie-Wei Chen / Xiao-Sheng He / Hui-Zhong Zhang / Yi-Hong Ling / Jia-Huai Wen / Wei-Hao Deng / Peng Li / Jing-Ping Yun / Dan Xie / Mu-Yan Cai

EBioMedicine, Vol 28, Iss C, Pp 62-

2018 Volume 69

Abstract: SATB2 (Special AT-rich sequence-binding protein 2) has recently been shown to be a specific biomarker of colorectal cancer (CRC). The aim of this study was to investigate the diagnostic potential of SATB2 as a means of detecting a CRC origin for liver ... ...

Abstract	SATB2 (Special AT-rich sequence-binding protein 2) has recently been shown to be a specific biomarker of colorectal cancer (CRC). The aim of this study was to investigate the diagnostic potential of SATB2 as a means of detecting a CRC origin for liver metastases. SATB2 expression was examined in a resection cohort of 101 CRC and 273 non-CRC adenocarcinoma samples using immunohistochemistry (IHC). The diagnostic accuracy of CRC origins of liver metastases based on SATB2 and a three marker panel of SATB2, CK20 and CDX2 was evaluated using an independent cohort of 192 liver biopsies. IHC showed 97 of the 101 (96.0%) primary CRC samples were SATB2 positive, compared to only 6 of the 273 (2.1%) samples of other cancer types. The sensitivity, specificity and AUC values of SATB2 expression in resection samples were 97%, 97.1% and 0.977, respectively. Meanwhile, for the liver biopsy samples, the sensitivity, specificity and AUC values of a CRC liver metastases was 92.2%, 97.8% and 0.948 for SATB2, 95.1%, 91.0% and 0.959 for CK20, and 100%, 85.4% and 0.976 for CDX2, respectively. Further analysis demonstrated that all three-marker positivity was detected in 92/103 (89.3%) CRC and 2/89 (2.2%) non-CRC liver metastases sampled by biopsy. Our findings suggest that SATB2, as measured by IHC, could serve as a promising diagnostic biomarker of CRC metastases. Combining evaluation of SATB2 with CK20 and CDX2 to form a three marker panel further improved the detection of metastatic CRCs in liver biopsy tissues.
Keywords	Diagnostic biomarker ; Metastatic colorectal cancer ; Liver biopsy ; SATB2 ; Medicine ; R ; Medicine (General) ; R5-920
Subject code	610
Language	English
Publishing date	2018-02-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: [Clinical significance of bronchial reversibility test in the treatment of childhood asthma].

Li, Yun / Liu, Dan / Yi, Hong-Ling / Guo, Chun / Chen, Min / Li, Wen-Kai

Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics

2013 Volume 15, Issue 2, Page(s) 105–108

Abstract: Objective: To observe changes in the lung function of asthmatic children with different symptoms during treatment, and to investigate the clinical significance of bronchial reversibility test in the treatment of asthma in children.: Methods: A total ... ...

Abstract	Objective: To observe changes in the lung function of asthmatic children with different symptoms during treatment, and to investigate the clinical significance of bronchial reversibility test in the treatment of asthma in children. Methods: A total of 417 asthmatic children were treated by salmeterol/fluticasone inhalation for more than 3 months. These patients were divided into asymptomatic, single cough, paroxysmal cough and wheeze (cough+wheeze or wheeze alone) groups based on the symptoms when they revisited the clinic. Thirty-four healthy children were used as a control group. All children underwent bronchial reversibility test using nebulized salbutamol. Lung function testing was performed before and after the test. Results: After nebulization of salbutamol, each asthma group showed significantly decreased rate of abnormal lung function and significantly increased forced expiratory volume in one second percent (FEV1%) predicted (P<0.05). Before salbutamol nebulization, the single cough, paroxysmal cough and wheeze groups had significantly higher rates of abnormal lung function and significantly lower FEV1% predicted than the control group (P<0.05). There were significant differences in the rate of abnormal lung function and FEV1% predicted among the asthma groups (P<0.05). After salbutamol nebulization, the paroxysmal cough and wheeze groups had significantly higher rates of abnormal lung function than the control group (P<0.05), but there were no significant differences between other asthma and control groups; the wheeze group had significantly lower FEV1% predicted than the control group, but no significant differences were found between other asthma and the control groups. The positive rate of bronchial reversibility test in each asthma group was significantly higher than in the control group (P<0.05). There were significant differences in the positive rate of the test between the asthma groups except between the asymptomatic and single cough groups (P<0.05). Conclusions: Asthmatic children with different symptoms demonstrate different lung functions during treatment. Bronchial reversibility test combined with lung function test is useful in assessing asthma control and guiding treatment.
MeSH term(s)	Administration, Inhalation ; Adolescent ; Albuterol/administration & dosage ; Albuterol/adverse effects ; Albuterol/analogs & derivatives ; Androstadienes/administration & dosage ; Androstadienes/adverse effects ; Asthma/drug therapy ; Asthma/physiopathology ; Bronchi/physiopathology ; Child ; Drug Combinations ; Female ; Fluticasone-Salmeterol Drug Combination ; Forced Expiratory Volume ; Humans ; Lung/physiopathology ; Male
Chemical Substances	Androstadienes ; Drug Combinations ; Fluticasone-Salmeterol Drug Combination ; Albuterol (QF8SVZ843E)
Language	Chinese
Publishing date	2013-02
Publishing country	China
Document type	Journal Article
ISSN	1008-8830
ISSN	1008-8830
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: [Results of skin prick test in young children with wheezing or allergic diseases].

Luo, Rui / Zhong, Li-Li / Yi, Hong-Ling / Tan, Yu-Pin / Chen, Min / Li, Yun

Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics

2012 Volume 14, Issue 4, Page(s) 282–284

Abstract: Objective: To study the characteristics of allergic reactions to common aeroallergens in young children with wheezing or allergic diseases by examining the results of skin prick test in children under 5 years old.: Methods: A total of 196 children ... ...

Abstract	Objective: To study the characteristics of allergic reactions to common aeroallergens in young children with wheezing or allergic diseases by examining the results of skin prick test in children under 5 years old. Methods: A total of 196 children under 5 years old, from a district of Changsha City sampled between September 1 to December 31, 2010, were assigned into two groups according to the presence of wheezing or allergic diseases: allergen screening (n=102) and control (n=94). Skin prick tests were performed on both groups. Results: The positive rate of skin prick test in the allergen screening group was 61.8% (63/102), and this was significantly higher than in the control group (9.6%, 9/94; P<0.05). In the allergen screening group, the positive rate of skin prick test in children with both recurrent wheezing and allergic rhinitis was significantly higher than in children with wheezing alone (P<0.05). The frequency of wheezing was positively correlated with a positive skin prick test (r=0.91; P<0.05). The positive rate of skin prick test for mites was significantly higher than for other aeroallergens (24.2% vs 3.5%; P<0.05) in the allergen screening group. Skin prick testing of the children for dermatophagoides farinae showed a higher positive rate than for dermatophagoides pteronyssinus (50.0% vs 14.7%; P<0.05). Conclusions: Wheezing in early childhood may be associated with the occurrence of asthma. Skin prick testing contributes to the diagnosis of allergic diseases and assessment of allergic reactions to aeroallergens in children with wheezing.
MeSH term(s)	Asthma/etiology ; Child, Preschool ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Respiratory Sounds/diagnosis ; Respiratory Sounds/immunology ; Rhinitis, Allergic, Perennial/diagnosis ; Rhinitis, Allergic, Perennial/immunology ; Rhinitis, Allergic, Seasonal/diagnosis ; Rhinitis, Allergic, Seasonal/immunology ; Skin Tests
Language	Chinese
Publishing date	2012-04
Publishing country	China
Document type	English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1008-8830
ISSN	1008-8830
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: [A preliminary study on the disappearance time of influenza virus antigen].

Chen, Hao-Feng / Zhang, Li-Li / Fang, Yi-Bing / Chen, Min / Guo, Chun / Yi, Hong-Ling / Tao, Mei-Ting / Li, Yan / Dai, Chu-Feng

Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics

2017 Volume 19, Issue 5, Page(s) 564–566

Abstract: Objective: To investigate the antigen clearance time, time to symptom disappearance, and the association between them using immunofluorescence assay for dynamic monitoring of influenza virus antigen in children with influenza.: Methods: A total of 1 ... ...

Abstract	Objective: To investigate the antigen clearance time, time to symptom disappearance, and the association between them using immunofluorescence assay for dynamic monitoring of influenza virus antigen in children with influenza. Methods: A total of 1 063 children suspected of influenza who visited the Hunan People's Hospital from March to April, 2016 were enrolled. The influenza A/B virus antigen detection kit (immunofluorescence assay) was used for influenza virus antigen detection. The children with positive results were given oseltamivir as the antiviral therapy and were asked to re-examine influenza virus antigen at 5, 5-7, and 7 days after onset. Results: Of all children suspected of influenza, 560 (52.68%) had an influenza virus infection. A total of 215 children with influenza virus infection were followed up. The clearance rate of influenza virus antigen was 9.8% (21 cases) within 5 days after onset. The cumulative clearance rate of influenza virus antigen was 32.1% (69 cases) within 5-7 days, and 98.1% (211 cases) within 7-10 days after onset. Among these children, 6 children (2.8%) achieved the improvement in clinical symptoms within 3 days after onset. The cumulative rate of symptom improvement was 84.7% (182 cases) within 3-5 days after onset, and 100% achieved the improvement after 5 days of onset. Conclusions: The time to improvement in symptoms after treatment is earlier than antigen clearance time. Almost all of the children achieve influenza virus antigen clearance 7-10 days after onset. Therefore, it is relatively safe for children to go back to school within 7-10 days after onset when symptoms disappear.
MeSH term(s)	Antigens, Viral/blood ; Child ; Child, Preschool ; Female ; Fluorescent Antibody Technique ; Humans ; Infant ; Influenza A virus/immunology ; Influenza B virus/immunology ; Male ; Time Factors
Chemical Substances	Antigens, Viral
Language	Chinese
Publishing date	2017-05-10
Publishing country	China
Document type	Journal Article
ISSN	1008-8830
ISSN	1008-8830
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Article: [Comparison of clinical data of childhood asthma in different periods in Changsha].

Li, Yun / Wang, Tang / Huang, Han / Yi, Hong-Ling

Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics

2007 Volume 9, Issue 6, Page(s) 540–542

Abstract: Objective: The incidence of childhood asthma is growing. This study aimed to find the changes of clinical features of childhood asthma over a 20-year period.: Methods: The medical data of 200 children with asthma between May 1986 and February 1988 ( ... ...

Abstract	Objective: The incidence of childhood asthma is growing. This study aimed to find the changes of clinical features of childhood asthma over a 20-year period. Methods: The medical data of 200 children with asthma between May 1986 and February 1988 (group 1) and of another 200 asthmatic children between June 2005 and May 2006 (group 2) were studied retrospectively. Results: Sixty-seven patients (33.5%) in group 1 and 128 patients (64.0%) in group 2 had concomitant allergic rhinitis (P < 0.01). The incidence of exercise-induced asthma, drug allergy and irritating smell allergy was significantly higher in group 2 than in group 1 (P < 0.05). Twenty-nine patients (14.5%) in group 1 and 112 patients (56.0%) in group 2 re-visited to the clinic (P < 0.01). The patients in group 1 received nonspecific immune and desensitizing therapy but those in group 2 received inhaled glucocorticoid therapy during the remission stage. After 3 months treatment, asthma was in under control in 11 patients (37.9%, 11/29) in group 1 and in 93 patients in group 2 (83.0%, 93/112) (P < 0.01). Conclusions: The incidence of concomitant anaphylaxis increased in asthmatic children over a 20-year period. The compliance of re-examination has increased in asthmatic children in recent years and this may be associated with a better efficacy of inhaled glucocorticosteroid therapy.
MeSH term(s)	Asthma/epidemiology ; Asthma/etiology ; Asthma/therapy ; Child ; Child, Preschool ; China/epidemiology ; Female ; Humans ; Incidence ; Male
Language	Chinese
Publishing date	2007-12
Publishing country	China
Document type	Comparative Study ; English Abstract ; Journal Article
ISSN	1008-8830
ISSN	1008-8830
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