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  1. Article ; Online: Identification of a Novel Peptide with Alcohol Dehydrogenase Activating Ability from Ethanol-Induced

    Chen, Sisi / Yi, Juanjuan / Kang, Qiaozhen / Song, Mo / Raubenheimer, David / Lu, Jike

    Journal of agricultural and food chemistry

    2024  Volume 72, Issue 11, Page(s) 5746–5756

    Abstract: Alcohol dehydrogenase (ADH) is a crucial rate-limiting enzyme in alcohol metabolism. Our previous research found that ethanol-induced intracellular extracts ... ...

    Abstract Alcohol dehydrogenase (ADH) is a crucial rate-limiting enzyme in alcohol metabolism. Our previous research found that ethanol-induced intracellular extracts of
    MeSH term(s) Mice ; Animals ; Ethanol/metabolism ; Lactococcus lactis/metabolism ; Blood Alcohol Content ; Alcohol Dehydrogenase/metabolism ; Kelch-Like ECH-Associated Protein 1/metabolism ; Molecular Docking Simulation ; NF-E2-Related Factor 2/metabolism ; Liver/metabolism
    Chemical Substances Ethanol (3K9958V90M) ; Blood Alcohol Content ; Alcohol Dehydrogenase (EC 1.1.1.1) ; Kelch-Like ECH-Associated Protein 1 ; NF-E2-Related Factor 2
    Language English
    Publishing date 2024-03-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.3c07632
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Cognition of polysaccharides from confusion to clarity: when the next "omic" will come?

    Cui, Yinxin / Liu, Xin / Yi, Juanjuan / Kang, Qiaozhen / Hao, Limin / Lu, Jike

    Critical Reviews in Food Science and Nutrition. 2023 Aug. 7, v. 63, no. 20 p.4728-4743

    2023  

    Abstract: With the accelerated pace of modern life, people are facing more and more health pressure. The study of polysaccharides seemed a good choice as a potential treasure trove. Polysaccharides, one of the four basic substances (proteins, nucleic acids, lipids ...

    Abstract With the accelerated pace of modern life, people are facing more and more health pressure. The study of polysaccharides seemed a good choice as a potential treasure trove. Polysaccharides, one of the four basic substances (proteins, nucleic acids, lipids and carbohydrates) that constitute life activities, are obviously an underrated macromolecular substance with great potential. Compared with protein and nucleic acid, the research of polysaccharides is still in the primary stage. The relationship between structure and function of polysaccharides is not clear. In this review, we highlighted the main methods of extraction, purification and structure identification of polysaccharides; summarized their biological activities including immunoregulation, hypoglycemic, anti-tumor, anti-virus, anti-coagulation, and so on. Particularly, the relationship between their structures and activities was described. In addition, the applications of polysaccharides in health food, medicine and cosmetics were also reviewed. This review can help polysaccharide researchers quickly understand the whole process of polysaccharides research, and also provide a reference for the comprehensive utilization of polysaccharides. We need to standardize the research of polysaccharides to make the experimental data more universal, and take it as important references in the review process. Glycomic may appear as the next "omic" after genomic and proteomic in the future. This review provides support for the advancement of glycomics.
    Keywords cognition ; cosmetics ; food science ; glycomics ; health foods ; immunomodulation ; medicine ; nucleic acids ; nutrition ; polysaccharides ; proteomics ; Biological activities ; purification ; structure-activity relationship ; structural characterization
    Language English
    Dates of publication 2023-0807
    Size p. 4728-4743.
    Publishing place Taylor & Francis
    Document type Article ; Online
    ZDB-ID 1037504-1
    ISSN 1549-7852 ; 1040-8398
    ISSN (online) 1549-7852
    ISSN 1040-8398
    DOI 10.1080/10408398.2021.2007045
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  3. Article ; Online: Nanoparticles prepared by polysaccharides extracted from Biyang floral mushroom loaded with resveratrol: Characterization, bioactivity and release behavior under in vitro digestion

    Liu, Keke / Liu, Yongqi / Lu, Jike / Liu, Xin / Hao, Limin / Yi, Juanjuan

    Food Chemistry. 2023 Nov., v. 426 p.136612-

    2023  

    Abstract: Resveratrol (RES) is a common active factor in the functional food field, but poor water solubility and low bioavailability have limited its application. In the present study, the novel nanoparticles (RES-CBFMP NPs) using floral mushroom polysaccharide ... ...

    Abstract Resveratrol (RES) is a common active factor in the functional food field, but poor water solubility and low bioavailability have limited its application. In the present study, the novel nanoparticles (RES-CBFMP NPs) using floral mushroom polysaccharide as the wall material have been developed for delivering RES, aiming to overcome its application shortcomings. After ratio optimization, RES-CBFMP NPs (RES-CBFMP,1:8 w/w), which combined through the hydrogen bonds between RES and CBFMP, showed the best overall performance, with the encapsulation efficiency (EE) of 49.74 ± 0.16%, loading efficiency (LE) of 5.53 ± 0.02%, particle size of 158.56 ± 1.97 nm and zeta-potential of −17.56 ± 0.24 mV. In addition, RES-CBFMP NPs exhibited good physicochemical stabilities, sustained gastrointestinal digestive release property, as well as improved in vitro antioxidant and anticancer activities. This study may contribute to the development of RES oral delivery systems and the application of hydrophobic active molecules in the functional food field.
    Keywords antioxidants ; bioactive properties ; bioavailability ; digestion ; encapsulation ; food chemistry ; functional foods ; gastrointestinal system ; hydrogen ; hydrophobicity ; mushrooms ; nanoparticles ; particle size ; polysaccharides ; resveratrol ; water solubility ; zeta potential ; Biyang floral mushroom polysaccharides ; Physicochemical stability ; Sustained release ; Bioactivity
    Language English
    Dates of publication 2023-11
    Publishing place Elsevier Ltd
    Document type Article ; Online
    ZDB-ID 243123-3
    ISSN 1873-7072 ; 0308-8146
    ISSN (online) 1873-7072
    ISSN 0308-8146
    DOI 10.1016/j.foodchem.2023.136612
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  4. Article: A Key Factor for Psychosomatic Burden of Frontline Medical Staff: Occupational Pressure During the COVID-19 Pandemic in China.

    Yi, Juanjuan / Kang, Lijing / Li, Jun / Gu, Jianfang

    Frontiers in psychiatry

    2021  Volume 11, Page(s) 590101

    Abstract: The global outbreak of COVID-19 has severely affected the entire population, especially healthcare staff on the frontline, who bear heavy psychosomatic burdens. A cross-sectional study was conducted with 723 participants in China from April 26 to May 9, ... ...

    Abstract The global outbreak of COVID-19 has severely affected the entire population, especially healthcare staff on the frontline, who bear heavy psychosomatic burdens. A cross-sectional study was conducted with 723 participants in China from April 26 to May 9, 2020. We evaluated the psychosomatic status, including depression, anxiety, quality of life, somatic symptoms, stress, sleep disturbances, and posttraumatic stress symptoms in different exposure groups. We explored the risk factors that affect psychosomatic burdens and analyzed the relationship between psychosomatic problems and medical occupations. We found that the psychosomatic burdens of medical staff were significantly greater than those of non-medical staff (
    Language English
    Publishing date 2021-01-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2564218-2
    ISSN 1664-0640
    ISSN 1664-0640
    DOI 10.3389/fpsyt.2020.590101
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Structural characterization of a glycoprotein from white jade snails (Achatina Fulica) and its wound healing activity.

    Li, Zhipeng / Song, Yiming / Ling, Yunying / Liu, Yingxin / Yi, Juanjuan / Hao, Limin / Zhu, Jiaqing / Kang, Qiaozhen / Huang, Jinyong / Lu, Jike

    International journal of biological macromolecules

    2024  Volume 263, Issue Pt 2, Page(s) 130161

    Abstract: Snail mucus is rich in proteins and polysaccharides, which has been proved to promote wound healing in mice in our previous research. The aim of this study was to investigate the effective component in snail mucus that can exert the wound healing ... ...

    Abstract Snail mucus is rich in proteins and polysaccharides, which has been proved to promote wound healing in mice in our previous research. The aim of this study was to investigate the effective component in snail mucus that can exert the wound healing potential and its structural characterization. Here, the glycoprotein from the snail mucus (SM1S) was obtained by DEAE-Sepharose Fast Flow and Sephacryl S-300 columns. The structural characteristics of SM1S were investigated via chromatographic techniques, periodic acid oxidation, FT-IR spectroscopy and NMR spectroscopy. Results showed that SM1S was a glycoprotein with a molecular weight of 3.8 kDa (83.23 %), consists of mannose, glucuronic acid, glucose, galactose, xylose, arabinose, fucose at a ratio of 13.180:4.875:1043.173:7.552:1:3.501:2.058. In addition, the periodic acid oxidation and NMR analysis showed that SM1S contained 1,6-glycosidic bonds, and might also contain 1 → 4 and 1 → 2 glycosidic or 1 → 3 glycosidic bonds. Furthermore, the migration experiment of human skin fibroblasts in vitro suggested that SM1S had a good effect to accelerate the scratch healing of cells. This study suggested that SM1S may be a prospective candidate as a natural wound dressing for the development of snail mucus products.
    MeSH term(s) Animals ; Humans ; Mice ; Spectroscopy, Fourier Transform Infrared ; Periodic Acid ; Polysaccharides/pharmacology ; Polysaccharides/chemistry ; Glycoproteins ; Wound Healing ; Snails
    Chemical Substances Periodic Acid (10450-60-9) ; Polysaccharides ; Glycoproteins
    Language English
    Publishing date 2024-02-16
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2024.130161
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  6. Article ; Online: Probiotic properties and proteomics analysis of ethanol-induced Lactococcus lactis subsp. lactis IL1403.

    Chen, Sisi / Yi, Juanjuan / Suo, Keke / Kang, Qiaozhen / Lu, Laizheng / Lu, Jike

    World journal of microbiology & biotechnology

    2023  Volume 39, Issue 8, Page(s) 197

    Abstract: Our previous study indicated that ethanol-induced intracellular extracts (E-IEs) of Lactococcus lactis subsp. Lactis IL1403 (L. lactis IL1403) alleviated hangovers more effectively in mice than untreated intracellular extracts (U-IEs), but the material ... ...

    Abstract Our previous study indicated that ethanol-induced intracellular extracts (E-IEs) of Lactococcus lactis subsp. Lactis IL1403 (L. lactis IL1403) alleviated hangovers more effectively in mice than untreated intracellular extracts (U-IEs), but the material basis was unclear. Considering that stress-related proteins might play a significant role, the effects of ethanol induction on probiotic properties of L. lactis IL1403 and the associated stress response mechanism were initially explored in this study. E-IEs of L. lactis IL1403 showed better biological activities, significantly increased bacteria survival rates in oxidative stress environments, increased ADH activity, and enhanced proliferation in RAW264.7 and AML-12 cells. Proteomic analyses revealed that 414 proteins were significantly changed in response to ethanol induction. The expression of proteins involved in the universal stress response, DNA repair, oxidative stress response, and ethanol metabolism was rapidly upregulated under ethanol stress, and quantitative real-time PCR (qRT-PCR) results were consistent with proteomic data. KEGG pathway analysis indicated that citrate metabolism, starch and sucrose metabolism, and pyruvate metabolism were significantly enriched during ethanol stress to increase energy requirements and survival rates of stressed cells. Based on this observation, the active induction is an effective strategy for increasing the biological activity of L. lactis IL1403. Exploring the molecular mechanism and material basis of their functions in vivo can help us understand the adaptive regulatory mechanism of microorganisms.
    MeSH term(s) Animals ; Mice ; Lactococcus lactis/genetics ; Lactococcus lactis/metabolism ; Ethanol/metabolism ; Proteomics
    Chemical Substances Ethanol (3K9958V90M)
    Language English
    Publishing date 2023-05-15
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1499109-3
    ISSN 1573-0972 ; 0959-3993
    ISSN (online) 1573-0972
    ISSN 0959-3993
    DOI 10.1007/s11274-023-03627-y
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  7. Article ; Online: Nanoparticles prepared by polysaccharides extracted from Biyang floral mushroom loaded with resveratrol: Characterization, bioactivity and release behavior under in vitro digestion.

    Liu, Keke / Liu, Yongqi / Lu, Jike / Liu, Xin / Hao, Limin / Yi, Juanjuan

    Food chemistry

    2023  Volume 426, Page(s) 136612

    Abstract: Resveratrol (RES) is a common active factor in the functional food field, but poor water solubility and low bioavailability have limited its application. In the present study, the novel nanoparticles (RES-CBFMP NPs) using floral mushroom polysaccharide ... ...

    Abstract Resveratrol (RES) is a common active factor in the functional food field, but poor water solubility and low bioavailability have limited its application. In the present study, the novel nanoparticles (RES-CBFMP NPs) using floral mushroom polysaccharide as the wall material have been developed for delivering RES, aiming to overcome its application shortcomings. After ratio optimization, RES-CBFMP NPs (RES-CBFMP,1:8 w/w), which combined through the hydrogen bonds between RES and CBFMP, showed the best overall performance, with the encapsulation efficiency (EE) of 49.74 ± 0.16%, loading efficiency (LE) of 5.53 ± 0.02%, particle size of 158.56 ± 1.97 nm and zeta-potential of -17.56 ± 0.24 mV. In addition, RES-CBFMP NPs exhibited good physicochemical stabilities, sustained gastrointestinal digestive release property, as well as improved in vitro antioxidant and anticancer activities. This study may contribute to the development of RES oral delivery systems and the application of hydrophobic active molecules in the functional food field.
    MeSH term(s) Resveratrol/chemistry ; Agaricales ; Antioxidants/chemistry ; Nanoparticles/chemistry ; Digestion ; Particle Size ; Drug Carriers/chemistry
    Chemical Substances Resveratrol (Q369O8926L) ; Antioxidants ; Drug Carriers
    Language English
    Publishing date 2023-06-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 243123-3
    ISSN 1873-7072 ; 0308-8146
    ISSN (online) 1873-7072
    ISSN 0308-8146
    DOI 10.1016/j.foodchem.2023.136612
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  8. Article ; Online: Lef1

    Yang, Min / Huang, Xueshan / Shen, Fang / Yi, Juanjuan / Meng, Yanni / Chen, Yanping

    Experimental lung research

    2022  Volume 48, Issue 7-8, Page(s) 213–223

    Abstract: Purpose: Bronchopulmonary dysplasia (BPD) is a long-term respiratory condition. More than a quarter of extremely premature newborns are harmed by BPD. At present, there are no apparent effective drugs or treatments for the condition. In this study, we ... ...

    Abstract Purpose: Bronchopulmonary dysplasia (BPD) is a long-term respiratory condition. More than a quarter of extremely premature newborns are harmed by BPD. At present, there are no apparent effective drugs or treatments for the condition. In this study, we aimed to investigate the functional role and mechanism of lymphoid enhancer-binding factor 1 (
    Materials and methods: Blood samples from BPD patients and healthy volunteers were gathered, and an
    Results: The mRNA levels of
    Conclusion: Klf4
    MeSH term(s) Alveolar Epithelial Cells/metabolism ; Bronchopulmonary Dysplasia/metabolism ; Cell Hypoxia ; Humans ; Infant, Newborn ; Kruppel-Like Factor 4/genetics ; Kruppel-Like Factor 4/metabolism ; Lymphoid Enhancer-Binding Factor 1/genetics ; Lymphoid Enhancer-Binding Factor 1/metabolism
    Chemical Substances KLF4 protein, human ; Kruppel-Like Factor 4 ; LEF1 protein, human ; Lymphoid Enhancer-Binding Factor 1
    Language English
    Publishing date 2022-08-11
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 603791-4
    ISSN 1521-0499 ; 0190-2148
    ISSN (online) 1521-0499
    ISSN 0190-2148
    DOI 10.1080/01902148.2022.2108945
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  9. Article ; Online: Characterization and correlation of bacterial community and volatile flavor compounds in xiguajiang, a Chinese traditional fermented condiment

    Zhao, Changcheng / Tian, Zhiyuan / Yi, Juanjuan / Shi, Yanling / Zhu, Jiaqing / Ji, Zhengze / Chen, Sisi / Kang, Qiaozhen / Lu, Jike

    Food Research International. 2022 Dec., v. 162 p.111904-

    2022  

    Abstract: Xiguajiang (XGJ) is one kind of Chinese traditionally fermented soybean food. The aim of this study was to identify core bacterial communities and volatile compounds and explore their relationships in XGJ samples obtained from different manufacturers. ... ...

    Abstract Xiguajiang (XGJ) is one kind of Chinese traditionally fermented soybean food. The aim of this study was to identify core bacterial communities and volatile compounds and explore their relationships in XGJ samples obtained from different manufacturers. Results showed that Bacillus, Staphylococcus, Weissella, and Chromohalobacter were the predominant bacterial genus, although their relative abundance is quite diverse. Larger relative contents of esters and alcohols were detected in XGJ. Moreover, the results of E-nose analysis indicated that nitrogen oxides compounds, pyrazines, and ketones compounds also played a critical role in XGJ unique flavor. The correlation analysis suggested that 3-methyl-butanol, ethoxybenzene, ethyl acetate, acetaldehyde, and 2-(4-methyl-3-cyclohexen-1-yl)-2-propanyl acetate had a significant correlation with Enterobacter, Clostridium, Pseudomonas, Streptomyces, Weissella, Staphylococcus, and Bacillus. These results may provide vital information to understand the role of the microbiota in developing flavor in XGJ products, and improve the quality and safety of XGJ production in industries.
    Keywords Chromohalobacter ; Clostridium ; Enterobacter ; Pseudomonas ; Staphylococcus ; Streptomyces ; Weissella ; acetaldehyde ; bacterial communities ; condiments ; electronic nose ; ethyl acetate ; fermentation ; flavor ; food research ; microorganisms ; nitrogen ; pyrazines ; soybeans ; Xiguajiang ; Microbial community ; Volatile compounds ; GC–MS analysis ; Correlation analysis
    Language English
    Dates of publication 2022-12
    Publishing place Elsevier Ltd
    Document type Article ; Online
    Note Pre-press version
    ZDB-ID 1111695-x
    ISSN 1873-7145 ; 0963-9969
    ISSN (online) 1873-7145
    ISSN 0963-9969
    DOI 10.1016/j.foodres.2022.111904
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  10. Article: Characterization of physicochemical properties, microbial diversity and volatile compounds of traditional fermented soybean paste in Henan province of China

    Tian, Zhiyuan / Ameer, Kashif / Shi, Yanling / Yi, Juanjuan / Zhu, Jiaqing / Kang, Qiaozhen / Lu, Jike / Zhao, Changcheng

    Food bioscience. 2022 Dec., v. 50

    2022  

    Abstract: This study aimed to investigate the changes in microbial diversity, and volatile compounds of traditional fermented soybean paste originated from Henan province of China using 16S rRNA sequencing, headspace solid phase microextraction-gas chromatography/ ... ...

    Abstract This study aimed to investigate the changes in microbial diversity, and volatile compounds of traditional fermented soybean paste originated from Henan province of China using 16S rRNA sequencing, headspace solid phase microextraction-gas chromatography/mass spectrometry (HS-SPME-GC/MS), and electronic nose (E-nose). The amino acid nitrogen and reducing sugar contents ranged between 1.18 and 1.58 g/100 g and 2.29 and 3.74 mg/g, respectively. The results showed that fermented soybean paste exhibited the highest amount of glutamate and aspartate amino acids. Approximately 112 volatile compounds were detected in all SP samples comprising 10 alcohols, 19 esters, 10 acidic compounds, and 21 heterocyclic compounds. Furthermore, 16S rRNA sequencing indicated that Enterobacter, Bacillus, Staphylococcus, and Enterococcus were the predominant bacteria in fermented soybean paste samples. Moreover, network analysis revealed that Klebsiella, Bacillus, and Lactobacillus positively correlated with H-fluorendimethyl, octenol, and benzaldehyde. These findings helped in understanding the formation of volatile flavoring compounds during SP fermentation.
    Keywords Enterobacter ; Enterococcus ; Klebsiella ; Lactobacillus ; Staphylococcus ; aspartic acid ; benzaldehyde ; chromatography ; electronic nose ; fermentation ; glutamic acid ; headspace analysis ; heterocyclic compounds ; mass spectrometry ; nitrogen ; octenol ; soybean paste ; sugars ; China
    Language English
    Dates of publication 2022-12
    Publishing place Elsevier Ltd
    Document type Article
    ISSN 2212-4292
    DOI 10.1016/j.fbio.2022.102045
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