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Article ; Online: Harnessing synthetic biology for sustainable biomining with Fe/S-oxidizing microbes

Jinjin Chen / Yilan Liu / Patrick Diep / Radhakrishnan Mahadevan

Frontiers in Bioengineering and Biotechnology, Vol

2022 Volume 10

Abstract: Biomining is a biotechnological approach where microorganisms are used to recover metals from ores and waste materials. While biomining applications are motivated by critical issues related to the climate crisis (e.g., habitat destruction due to mine ... ...

Abstract	Biomining is a biotechnological approach where microorganisms are used to recover metals from ores and waste materials. While biomining applications are motivated by critical issues related to the climate crisis (e.g., habitat destruction due to mine effluent pollution, metal supply chains, increasing demands for cleantech-critical metals), its drawbacks hinder its widespread commercial applications: lengthy processing times, low recovery, and metal selectivity. Advances in synthetic biology provide an opportunity to engineer iron/sulfur-oxidizing microbes to address these limitations. In this forum, we review recent progress in synthetic biology-enhanced biomining with iron/sulfur-oxidizing microbes and delineate future research avenues.
Keywords	synthetic biology ; Fe/S-oxidizing microbes ; biomining ; design-build-test-learn (DBTL) cycle ; CRISPR ; Biotechnology ; TP248.13-248.65
Language	English
Publishing date	2022-09-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Patients’ caring experience during procedures under regional anesthesia in Mainland China

Rui Chen / Ying Chen / Meng Yang / Yilan Liu / Xinhui Zhang / Jianlei Li / Xue Yang / Yufeng Liao / Guijuan Du / Xiangyang Cao

Heliyon, Vol 9, Iss 10, Pp e20330- (2023)

A phenomenology study

2023

Abstract: Background: Although regional anesthesia is common, the procedure results in feelings of uncertainty and anxiety in some patients. Increased care is needed for these patients under general anesthesia. Few studies have focused on the intraoperative caring ...

Abstract	Background: Although regional anesthesia is common, the procedure results in feelings of uncertainty and anxiety in some patients. Increased care is needed for these patients under general anesthesia. Few studies have focused on the intraoperative caring experience of patients during regional anesthesia. This study focused on the caring experience of patients during procedures involving regional anesthesia. Methods: The descriptive phenomenology method of Husserl was employed. Semi-structured interviews were conducted with a purposive sample in five Grade III-A hospitals in Zhengzhou City, Henan Province. The seven-step analysis method of Colaizzi was applied for the analysis, summation, and theme refinement of the interview data. Results: A total of 14 patients from five hospitals participated in the interviews. Four domains and 16 themes emerged during analysis: be informed (about the operation site, progression of the operation, informed in advance, receive explanation for abnormal experience); take care of my body (painless, gentle movements, special care); be protected (work seriously, favorable atmosphere, skilled, authority); and treated as an individual (pay attention, accompany, ask for opinions, encourage patient expression, humorous). Conclusion: Patients during procedure under regional anesthesia had specific caring experiences relative to other patients. Medical staff should recognize the importance of regional anesthesia patients’ intraoperative caring experience. Hospital administrators should offer support to allow healthcare staff to provide targeted caring for patients during procedure under regional anesthesia.
Keywords	Caring ; Procedure under regional anesthesia ; Patient ; Phenomenology study ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
Language	English
Publishing date	2023-10-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: An analysis of the influencing factors of depression in older adults under the home care model

Yanjie You / Lei Huang / Xiao Peng / Lulu Liao / Fengjian Zhang / Mingjiao Feng / Yuqin Chen / Hongwei Chang / Beirong Mo / Yilan Liu

Frontiers in Public Health, Vol

2023 Volume 11

Abstract: ObjectivesTo explore and analyze the influencing factors of depression in older adults living at home, so as to propose suggestions for improving the quality of older adults living at home.MethodsWe conducted a cross-sectional study on 498 older adults ... ...

Abstract	ObjectivesTo explore and analyze the influencing factors of depression in older adults living at home, so as to propose suggestions for improving the quality of older adults living at home.MethodsWe conducted a cross-sectional study on 498 older adults living at home based on questionnaire survey on the general information, daily living ability, health status, and care perception (including self-care, care for cohabitants, and care for non-cohabitants) of older adults living at home, as well as their willingness to help each other, and analyzed the influencing factors of depression among older adults living at home.ResultsThe results showed a willingness to help older adults, self-care, and total activities of daily living (ADL), health status was an influential factor for depression in older adults (p < 0.05).ConclusionIt aims to take targeted measures, such as encouraging older adults at home to actively participate in mutual assistance activities for older adults and care for themselves, so as to prevent and reduce the occurrence of depression in older adults.
Keywords	depression ; home-based care ; self-care ; population aging ; mutual help ; Public aspects of medicine ; RA1-1270
Subject code	300
Language	English
Publishing date	2023-11-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Quantitative temperature and relative humidity changes recorded by the Lake Cuoqia in the southeastern Tibetan Plateau during the past 300 years

Tianlong Yan / Can Zhang / Haixia Zhang / Xiaoshuang Sun / Yilan Liu / Ruikun Liu / Wei Zhang / Cheng Zhao

Frontiers in Ecology and Evolution, Vol

2023 Volume 11

Abstract: High-elevation lakes on the Tibetan Plateau have the advantage of sensitive response to climate changes. Multiple proxy records in lake sediments can provide a large amount of extractable information for paleoclimate reconstructions and assessing the ... ...

Abstract	High-elevation lakes on the Tibetan Plateau have the advantage of sensitive response to climate changes. Multiple proxy records in lake sediments can provide a large amount of extractable information for paleoclimate reconstructions and assessing the position of recent global warming within the context of natural climate variability. In this study, we reconstruct the climatic and environmental changes over the past 300 years from a remote alpine lake (Lake Cuoqia) in the southeastern Tibetan Plateau using multiple proxies including branched glycerol dialkyl glycerol tetraethers (brGDGTs), n-alkanes, elements, fatty acids and their hydrogen isotopes. Due to ice-cover nature of lake surface during winter, brGDGTs mainly reflect the variation in warm-season temperature from March to October, supported by nearby instrumental data. Our reconstructed high-resolution temperature showed a continuous cooling trend between 1700 and 1950 AD, followed by a rapid warming afterward, in parallel with other proxies such as n-alkanes and fatty acids in the same core, which is also consistent with previously published regional temperature records. The hydrogen isotope (δD) of fatty acids, similar to regional tree-ring δ18O, can record the history of atmospheric precipitation isotope and further indicate the variations of regional relative humidity. Our record exhibited a long-term decrease since 1700 AD, in accord with the decreasing lake level inferred from the ratio of Fe/Mn. The combined pattern of reconstructed temperature and relative humidity showed consistent changes before 1950 AD toward to a gradually cold-dry trend, whereas started to decouple afterward. Before 1950 AD, the declined temperature and relative humidity are mainly driven by insolation and thermal contrast between the Indian-Pacific Ocean and south Asian continent. After 1950 AD, decoupling of temperature and relative humidity may be related to the increased regional evaporation and human-induced emission of greenhouse gases and aerosol.
Keywords	quantitative temperature ; relative humidity ; brGDGTs ; hydrogen isotopes ; past 300 years ; Evolution ; QH359-425 ; Ecology ; QH540-549.5
Subject code	550
Language	English
Publishing date	2023-02-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Length of Stay, Hospital Costs and Mortality Associated With Comorbidity According to the Charlson Comorbidity Index in Immobile Patients After Ischemic Stroke in China

Hongpeng Liu / Baoyun Song / Jingfen Jin / Yilan Liu / Xianxiu Wen / Shouzhen Cheng / Stephen Nicholas / Elizabeth Maitland / Xinjuan Wu / Dawei Zhu

International Journal of Health Policy and Management, Vol 11, Iss 9, Pp 1780-

A National Study

2022 Volume 1787

Abstract: Background In this study, we examined the length of stay (LoS)-predictive comorbidities, hospital costs-predictive comorbidities, and mortality‐predictive comorbidities in immobile ischemic stroke (IS) patients; second, we used the Charlson Comorbidity ... ...

Abstract	Background In this study, we examined the length of stay (LoS)-predictive comorbidities, hospital costs-predictive comorbidities, and mortality‐predictive comorbidities in immobile ischemic stroke (IS) patients; second, we used the Charlson Comorbidity Index (CCI) to assess the association between comorbidity and the LoS and hospitalization costs of stroke; third, we assessed the magnitude of excess IS mortality related to comorbidities. Methods Between November 2015 and July 2017, 5114 patients hospitalized for IS in 25 general hospitals from six provinces in eastern, western, and central China were evaluated. LoS was the period from the date of admission to the date of discharge or date of death. Costs were collected from the hospital information system (HIS) after the enrolled patients were discharged or died in hospital. The HIS belongs to the hospital’s financial system, which records all the expenses of the patient during the hospital stay. Cause of death was recorded in the HIS for 90 days after admission regardless of whether death occurred before or after discharge. Using the CCI, a comorbidity index was categorized as zero, one, two, and three or more CCI diseases. A generalized linear model with a gamma distribution and a log link was used to assess the association of LoS and hospital costs with the comorbidity index. Kaplan–Meier survival curves was used to examine overall survival rates.Results We found that 55.2% of IS patients had a comorbidity. Prevalence of peripheral vascular disease (21.7%) and diabetes without end-organ damage (18.8%) were the major comorbidities. A high CCI=3+ score was an effective predictor of a high risk of longer LoS and death compared with a low CCI score; and CCI=2 score and CCI=3+ score were efficient predictors of a high risk of elevated hospital costs. Specifically, the most notable LoS-specific comorbidities, and cost-specific comorbidities was dementia, while the most notable mortality-specific comorbidities was moderate or severe renal disease. Conclusion CCI has ...
Keywords	stroke ; comorbidity ; mortality ; costs ; length of stay ; Public aspects of medicine ; RA1-1270
Subject code	610
Language	English
Publishing date	2022-09-01T00:00:00Z
Publisher	Kerman University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Hospitalization costs among immobile patients with hemorrhagic or ischemic stroke in China

Hongpeng Liu / Chen Zhu / Jing Cao / Jing Jiao / Baoyun Song / Jingfen Jin / Yilan Liu / Xianxiu Wen / Shouzhen Cheng / Xinjuan Wu

BMC Health Services Research, Vol 20, Iss 1, Pp 1-

a multicenter cross-sectional study

2020 Volume 11

Abstract: Abstract Background In this study, we aimed to analyze the hospitalization costs for immobile patients with hemorrhagic stroke (IHS) or ischemic stroke (IIS) in China and to determine the factors associated with hospitalization costs. Methods We ... ...

Abstract	Abstract Background In this study, we aimed to analyze the hospitalization costs for immobile patients with hemorrhagic stroke (IHS) or ischemic stroke (IIS) in China and to determine the factors associated with hospitalization costs. Methods We evaluated patients with IHS and IIS hospitalized between November 2015 and July 2016 in six provinces or municipality cities of China. Linear regression analysis was used to examine the association with hospitalization costs and predictors. Results In total, 1573 patients with IHS and 3143 with IIS were enrolled and analyzed. For IHS and IIS, the average length of stay (LoS) was 17.40 ± 12.3 and 14.47 ± 11.55 days. The duration of immobility was 12.11 ± 9.98 and 7.36 ± 9.77 days, respectively. Median hospitalization costs were RMB 47000.68 (interquartile range 19,827.37, 91,877.09) for IHS and RMB 16578.44 (IQR 7020.13, 36,357.65) for IIS. In both IHS and IIS groups, medicine fees accounted for more than one-third of hospitalization costs. Materials fees and medical service fees accounted for the second and third largest proportions of hospital charges in both groups. Linear regression analysis showed that LoS, hospital level, and previous surgery were key determinants of hospitalization costs in all immobile patients with stroke. Subgroup analysis indicated that hospital level was highly correlated with hospitalization costs for IHS whereas pneumonia and deep vein thrombosis were key factors associated with hospitalization costs for IIS. Conclusions We found that hospitalization costs were notably higher in IHS than IIS, and medicine fees accounted for the largest proportion of hospitalization costs in both patient groups, perhaps because most patients ended up with complications such as pneumonia thereby requiring more medications. LoS and hospital level may greatly affect hospitalization costs. Increasing the reimbursement ratio of medical insurance for patients with IHS is recommended. Decreasing medicine fees and LoS, preventing complications, and improving ...
Keywords	Hospitalization costs ; Stroke subtype ; Prospective observational study ; Multicenter ; China ; Public aspects of medicine ; RA1-1270
Subject code	616
Language	English
Publishing date	2020-09-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Risk factors for 3-month mortality in bedridden patients with hospital-acquired pneumonia

Jing Jiao / Zhen Li / Xinjuan Wu / Jing Cao / Ge Liu / Ying Liu / Fangfang Li / Chen Zhu / Baoyun Song / Jingfen Jin / Yilan Liu / Xianxiu Wen / Shouzhen Cheng / Xia Wan

PLoS ONE, Vol 16, Iss 3, p e

A multicentre prospective study.

2021 Volume 0249198

Abstract: Background Mortality among patients with hospital-acquired pneumonia (HAP) is quite high; however, information on risk factors for short-term mortality in this population remains limited. The aim of the current study was to identify the risk factors for ... ...

Abstract	Background Mortality among patients with hospital-acquired pneumonia (HAP) is quite high; however, information on risk factors for short-term mortality in this population remains limited. The aim of the current study was to identify the risk factors for mortality in bedridden patients with HAP during a 3-month observation period. Methods A secondary data analysis was conducted. In total, 1141 HAP cases from 25 hospitals were included in the analysis. Univariate and multilevel regression analyses were performed to identify the risk factors for mortality. Results During the 3-month observation period, there were 189 deaths among bedridden patients with HAP. The mortality rate in this study was 16.56%. Multilevel regression analysis showed that ventilator-associated pneumonia (OR = 2.034, 95%CI: 1.256, 3.296, p = 0.004), pressure injuries (OR = 2.202, 95%CI: 1.258, 3.852, p = 0.006), number of comorbidities (OR = 1.076, 95%CI: 1.016,1.140, p = 0.013) and adjusted Charlson Comorbidity Index score (OR = 1.210, 95%CI: 1.090, 1.343, p<0.001) were associated with an increased risk of mortality, while undergoing surgery with general anaesthesia (OR = 0.582, 95%CI: 0.368, 0.920, p = 0.021) was associated with a decreased risk of mortality. Conclusions The identification of risk factors associated with mortality is an important step towards individualizing care plans. Our findings may help healthcare workers select high-risk patients for specific interventions. Further study is needed to explore whether appropriate interventions against modifiable risk factors, such as reduced immobility complications or ventilator-associated pneumonia, could improve the prognoses.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Deciphering molecular details in the assembly of alpha-type carboxysome

Yilan Liu / Xinyuan He / Weiping Lim / Joshua Mueller / Justin Lawrie / Levi Kramer / Jiantao Guo / Wei Niu

Scientific Reports, Vol 8, Iss 1, Pp 1-

2018 Volume 10

Abstract: Abstract Bacterial microcompartments (BMCs) are promising natural protein structures for applications that require the segregation of certain metabolic functions or molecular species in a defined microenvironment. To understand how endogenous cargos are ... ...

Abstract	Abstract Bacterial microcompartments (BMCs) are promising natural protein structures for applications that require the segregation of certain metabolic functions or molecular species in a defined microenvironment. To understand how endogenous cargos are packaged inside the protein shell is key for using BMCs as nano-scale reactors or delivery vesicles. In this report, we studied the encapsulation of RuBisCO into the α-type carboxysome from Halothiobacillus neapolitan. Our experimental data revealed that the CsoS2 scaffold proteins engage RuBisCO enzyme through an interaction with the small subunit (CbbS). In addition, the N domain of the large subunit (CbbL) of RuBisCO interacts with all shell proteins that can form the hexamers. The binding affinity between the N domain of CbbL and one of the major shell proteins, CsoS1C, is within the submicromolar range. The absence of the N domain also prevented the encapsulation of the rest of the RuBisCO subunits. Our findings complete the picture of how RuBisCOs are encapsulated into the α-type carboxysome and provide insights for future studies and engineering of carboxysome as a protein shell.
Keywords	Medicine ; R ; Science ; Q
Subject code	612
Language	English
Publishing date	2018-10-01T00:00:00Z
Publisher	Nature Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Factors associated with death in bedridden patients in China

Jing Cao / Tiantian Wang / Zhen Li / Ge Liu / Ying Liu / Chen Zhu / Jing Jiao / Jiaqian Li / Fangfang Li / Hongpeng Liu / Huaping Liu / Baoyun Song / Jingfen Jin / Yilan Liu / Xianxiu Wen / Shouzhen Cheng / Xia Wan / Xinjuan Wu

PLoS ONE, Vol 15, Iss 1, p e

A longitudinal study.

2020 Volume 0228423

Abstract: BACKGROUND:Immobility is common and associated with adverse outcomes in hospitalized patients, especially older people. However, the factors contributing to mortality in bedridden patients are not well known. This study aimed to estimate short-term ... ...

Abstract	BACKGROUND:Immobility is common and associated with adverse outcomes in hospitalized patients, especially older people. However, the factors contributing to mortality in bedridden patients are not well known. This study aimed to estimate short-term mortality and analyze risk factors that affect the prognosis of bedridden patients. METHODS:This was a multicenter study in China involving 23,738 patients admitted to 25 hospitals between November 2015 and June 2016. All-cause mortality was recorded for 90 days after enrollment regardless of whether death occurred before or after discharge. Socio-demographic and clinical information was obtained from an electronic database. Univariate and multivariate Cox regression analysis was used to identify factors associated with mortality. RESULTS:In total, 23,738 hospitalized bedridden patients, there were 1,114 (4.7%) observed deaths. The overall mortality rate was therefore 4.7%. Of these, 318 (1.4%) died while hospitalized and 796 (3.4%) after discharge. The univariate Cox regression analysis showed that variables significantly associated with 90-day mortality included total time spent bedridden, urinary tract infection and pulmonary infection (p<0.05). The multivariate Cox regression analysis showed that the independent risk factors for death were age (adjusted hazard ratio [aHR] 1.006, 95% CI 1.000-1.011), and pulmonary infection (aHR 1.439, 95% CI 1.266-1.635). The hazard ratios for mortality were reduced with urinary tract infection and more time spent bedridden. CONCLUSIONS:The mortality after discharge was significantly higher than mortality in hospital. The factors affecting short-term mortality in bedridden patients included age, time spent bedridden, urinary tract infection and pulmonary infection. This suggests these factors may be potential predictors of mortality in bedridden patients. It is essential for medical staff to improve health education of patients and family members, pay more attention to follow up after discharge and meet care needs at home.
Keywords	Medicine ; R ; Science ; Q
Subject code	310
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli

Olajuyin, Ayobami Matthew / Jiangnan Tian / Jianmin Xing / Maohua Yang / Oluwatosin Adekunle Adaramoye / Tingzhen Mu / Yilan Liu

Bioresource technology. 2016 Aug., v. 214

2016

Abstract: Succinic acid, a C4 dicarboxylic acid is used in many fields such as food, agriculture, pharmaceutical and polymer industries. In this study, microbial production of succinic acid from Palmaria palmata was investigated for the first time. In engineered ... ...

Abstract	Succinic acid, a C4 dicarboxylic acid is used in many fields such as food, agriculture, pharmaceutical and polymer industries. In this study, microbial production of succinic acid from Palmaria palmata was investigated for the first time. In engineered Escherichia coli KLPPP, lactate dehydrogenase, pyruvate formate lyase, phosphotransacetylase–acetate kinase and pyruvate oxidase genes were deleted while phosphoenolpyruvate carboxykinase was overexpressed. The recombinant exhibited higher molar yield of succinic acid on galactose (1.20±0.02mol/mol) than glucose (0.48±0.03mol/mol). The concentration and molar yield of succinic acid were 22.40±0.12g/L and 1.13±0.02mol/mol total sugar respectively after 72h dual phase fermentation from P. palmata hydrolysate which composed of glucose (12.57±0.17g/L) and galactose (18.03±0.10g/L). The results demonstrate that P. palmata red macroalgae biomass represents a novel and an economically alternative feedstock for biochemicals production.
Keywords	biomass ; Escherichia coli ; feedstocks ; fermentation ; formates ; galactose ; gene overexpression ; genes ; glucose ; hydrolysates ; industry ; lactate dehydrogenase ; macroalgae ; metabolic engineering ; Palmaria ; polymers ; pyruvate kinase ; pyruvate oxidase ; pyruvic acid ; succinic acid
Language	English
Dates of publication	2016-08
Size	p. 653-659.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	1065195-0
ISSN	1873-2976 ; 0960-8524
ISSN (online)	1873-2976
ISSN	0960-8524
DOI	10.1016/j.biortech.2016.04.117
Database	NAL-Catalogue (AGRICOLA)

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