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Article ; Online: Circular RNA circRPS19 promotes chicken granulosa cell proliferation and steroid hormone synthesis by interrupting the miR-218-5p/INHBB axis.

Wei, Yuanhang / Shen, Xiaoxu / Zhao, Xiyu / He, Haorong / Zhang, Yao / Zhu, Qing / Yin, Huadong

2024 Volume 219, Page(s) 103–115

Abstract: Ovarian follicle development is an important physiological activity for females and makes great significance in maintaining female health and reproduction performance. The development of ovarian follicle is mainly affected by the granulosa cells (GCs), ... ...

Abstract	Ovarian follicle development is an important physiological activity for females and makes great significance in maintaining female health and reproduction performance. The development of ovarian follicle is mainly affected by the granulosa cells (GCs), whose growth is regulated by a variety of factors. Here, we identified a novel circular RNA (circRNA) derived from the Ribosomal protein S19 (RPS19) gene, named circRPS19, which is differentially expressed during chicken ovarian follicle development. Further explorations identified that circRPS19 promotes GCs proliferation and steroid hormone synthesis. Furthermore, circRPS19 was found to target and regulate miR-218-5p through a competitive manner with endogenous RNA (ceRNA). Functionals investigation revealed that miR-218-5p attenuates GCs proliferation and steroidogenesis, which is opposite to that of circRPS19. In addition, we also confirmed that circRPS19 upregulates the expression of Inhibin beta B subunit (INHBB) by binding with miR-218-5p to facilitate GCs proliferation and steroidogenesis. Overall, this study revealed that circRPS19 regulates GCs development by releasing the repression of miR-218-5p on INHBB, which suggests a novel mechanism in respect to circRNA and miRNA regulation in ovarian follicle development.
MeSH term(s)	Female ; Animals ; RNA, Circular/genetics ; RNA, Circular/metabolism ; Chickens/genetics ; Chickens/metabolism ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Granulosa Cells/metabolism ; Cell Proliferation ; Steroids/metabolism
Chemical Substances	RNA, Circular ; MicroRNAs ; Steroids
Language	English
Publishing date	2024-02-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	189232-0
ISSN	1879-3231 ; 0093-691X
ISSN (online)	1879-3231
ISSN	0093-691X
DOI	10.1016/j.theriogenology.2024.02.026
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Article: High expression circRALGPS2 in atretic follicle induces chicken granulosa cell apoptosis and autophagy via encoding a new protein.

He, Haorong / Wei, Yuanhang / Chen, Yuqi / Zhao, Xiyu / Shen, Xiaoxu / Zhu, Qing / Yin, Huadong

Journal of animal science and biotechnology

2024 Volume 15, Issue 1, Page(s) 42

Abstract: Background: The reproductive performance of chickens mainly depends on the development of follicles. Abnormal follicle development can lead to decreased reproductive performance and even ovarian disease among chickens. Chicken is the only non-human ... ...

Abstract	Background: The reproductive performance of chickens mainly depends on the development of follicles. Abnormal follicle development can lead to decreased reproductive performance and even ovarian disease among chickens. Chicken is the only non-human animal with a high incidence of spontaneous ovarian cancer. In recent years, the involvement of circRNAs in follicle development and atresia regulation has been confirmed. Results: In the present study, we used healthy and atretic chicken follicles for circRNA RNC-seq. The results showed differential expression of circRALGPS2. It was then confirmed that circRALGPS2 can translate into a protein, named circRALGPS2-212aa, which has IRES activity. Next, we found that circRALGPS2-212aa promotes apoptosis and autophagy in chicken granulosa cells by forming a complex with PARP1 and HMGB1. Conclusions: Our results revealed that circRALGPS2 can regulate chicken granulosa cell apoptosis and autophagy through the circRALGPS2-212aa/PARP1/HMGB1 axis.
Language	English
Publishing date	2024-03-11
Publishing country	England
Document type	Journal Article
ZDB-ID	2630162-3
ISSN	2049-1891 ; 1674-9782
ISSN (online)	2049-1891
ISSN	1674-9782
DOI	10.1186/s40104-024-01003-w
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Article ; Online: Effects of various selenium-enriched yeasts, selenomethionine, and nanoselenium on production performance, quality, and antioxidant capacity in laying hens.

Chen, Yuqi / He, Haorong / Bi, Xuejiao / Zhang, Yao / Yin, Huadong

Poultry science

2023 Volume 103, Issue 3, Page(s) 103387

Abstract: This study aimed to compare the effects of various selenium (Se) sources (2 mg/kg) on the performance, quality, and antioxidant capacity of laying hens as well as the Se content in their eggs and blood. We selected 720 34-wk-old Lohmann pink-shell laying ...

Abstract	This study aimed to compare the effects of various selenium (Se) sources (2 mg/kg) on the performance, quality, and antioxidant capacity of laying hens as well as the Se content in their eggs and blood. We selected 720 34-wk-old Lohmann pink-shell laying hens were randomly assigned into 6 groups and fed a basal diet (control) or a basal diet supplemented with various Se sources (Se-enriched yeast, SY-A, SY-C, SY-N; selenomethionine SM, nano-Se SN) for 16 wk. There were 10 replicates of 120 hens per group. Dietary Se supplementation increased the egg production rate of all laying hens. Egg and serum Se deposition was highest in the SM group. Yolk color scores of SY-A and SY-N groups were significantly lower than those of other groups (P < 0.01). The protein height and Haugh unit were significantly lower in the SN group than in the other groups (P < 0.05). The yolk height was significantly higher in the SN and SY-N groups than in the SY-A group (P < 0.05). Dietary supplementation of selenium can improve the antioxidant capacity of laying hens. The SOD content of SM group was significantly lower than that of SY-A and SN group (P < 0.05). The malondialdehyde (MDA) content was significantly higher in the SM group than in the SY-A group (P < 0.05). The present work empirically demonstrated that the production performance of laying hens supplemented with 2 mg/kg Se was superior to that of the hens receiving only a basal diet. The SY-C group exhibited the best production performance, the SY-A group had the highest antioxidant capacity, and the SM group produced eggs with the highest level of Se enrichment.
MeSH term(s)	Animals ; Female ; Antioxidants ; Chickens ; Ovum ; Saccharomyces cerevisiae ; Selenium/pharmacology ; Selenomethionine/pharmacology
Chemical Substances	Antioxidants ; Selenium (H6241UJ22B) ; Selenomethionine (964MRK2PEL)
Language	English
Publishing date	2023-12-16
Publishing country	England
Document type	Comparative Study ; Journal Article ; Randomized Controlled Trial, Veterinary
ZDB-ID	242586-5
ISSN	1525-3171 ; 0032-5791
ISSN (online)	1525-3171
ISSN	0032-5791
DOI	10.1016/j.psj.2023.103387
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Article ; Online: Quantitative proteomic and phosphoproteomic analysis of chicken skeletal muscle during embryonic development

Cui, Can / Yin, Huadong / Han, Shunshun / Zhang, Yao / Zhang, Yun / Zhu, Qing

Animal Biotechnology. 2023 Apr. 3, v. 34, no. 2 p.122-133

2023

Abstract: Skeletal muscle also plays a vital role in regulating the movement energy storage and health of metabolism. In order to investigate the expression profile of protein and phosphor-proteins in chicken skeletal muscle during embryonic development, we ... ...

Abstract	Skeletal muscle also plays a vital role in regulating the movement energy storage and health of metabolism. In order to investigate the expression profile of protein and phosphor-proteins in chicken skeletal muscle during embryonic development, we performed phosphor-proteomics analysis by label-free and TiO₂ enrichment strategy in chicken leg muscle tissues of at embryonic age embryo day 7(E7), E12, E17 and 3-day post-hatch (D3). The study led to the identification of 4332 proteins in the proteome and 1043 phosphorylation modification sites in the phosphorylated proteome, corresponding to 718 proteins (FC ≥ 2 or FC ≤ 0.5 and p < 0.05). The DEP-associated biological processes were involved in Focal adhesion, Glycolysis/gluconeogenesis, Arginine and proline metabolism by KEGG analysis. PPI analyses revealed that these DEPs TNNC1, TNNC2, TNNT2, TNNT3 and phosphorylated DEPs MYLPF interacted with involved pathways. Integrative analysis of proteome and phosphoproteome data found 324 common proteins, corresponding to 521 modification sites and Focal adhesion was the only pathway significantly enriched. These results provide a basis for further understanding the proteome and phosphoproteome and their regulatory biochemical pathways during the development of embryonic chicken skeletal muscle.
Keywords	arginine ; biotechnology ; chickens ; embryogenesis ; energy ; focal adhesions ; gluconeogenesis ; glycolysis ; muscles ; phosphoproteome ; phosphorylation ; proline ; proteomics ; skeletal muscle ; Proteome ; chicken ; embryo
Language	English
Dates of publication	2023-0403
Size	p. 122-133.
Publishing place	Taylor & Francis
Document type	Article ; Online
ZDB-ID	2043243-4
ISSN	1532-2378 ; 1049-5398
ISSN (online)	1532-2378
ISSN	1049-5398
DOI	10.1080/10495398.2021.1941071
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Article ; Online: Transcriptome profiling reveals SLC5A5 regulates chicken ovarian follicle granulosa cell proliferation, apoptosis, and steroid hormone synthesis.

Shen, Xiaoxu / Zhao, Xiyu / He, Haorong / Zhang, Yao / Zhu, Qing / Yin, Huadong

Poultry science

2023 Volume 103, Issue 1, Page(s) 103241

Abstract: The egg-laying performance of hens holds significant economic importance within the poultry industry. Broody inheritance of the parent stock of chickens can result in poor options for the improvement of egg production, and is a phenomenon influenced by ... ...

Abstract	The egg-laying performance of hens holds significant economic importance within the poultry industry. Broody inheritance of the parent stock of chickens can result in poor options for the improvement of egg production, and is a phenomenon influenced by multiple genetic factors. However, few studies have been conducted to delineate the molecular mechanism of ovarian regression in brooding chickens. Here, we explored the pivotal genes responsible for the regulation of ovarian follicles in laying hens, using RNA-sequencing analysis on the small ovarian follicles from broody and laying chickens. Sequencing data analysis revealed the differential expression of 200 genes, with a predominant enrichment in biological processes related to cell activation and metabolism. Among these genes, we focused on solute carrier family 5 member 5 (SLC5A5), which exhibited markedly higher RNA expression levels in follicles from laying compared with broody chickens. Subsequent cellular function studies with knockdown of SLC5A5 in chicken ovarian follicle granulosa cells (GCs) led to the down-regulation of genes associated with cell proliferation and steroid hormone synthesis, and concurrent promotion of gene expression linked to apoptosis. These findings indicated that SLC5A5 deficiency led to the inhibition of proliferation, steroid hormone synthesis and secretion, and promotion of apoptosis in chicken GCs. Our study demonstrated a pivotal role for SLC5A5 in the development and function of chicken GCs, shedding light on its potential significance in the broader context of chicken ovarian follicle development, and providing a prospective target to improve the egg-laying performance of chickens via molecular marker-assisted breeding technology.
MeSH term(s)	Animals ; Female ; Chickens/genetics ; Ovarian Follicle/physiology ; Granulosa Cells ; Gene Expression Profiling/veterinary ; Cell Proliferation ; Apoptosis ; Hormones/metabolism ; RNA/metabolism ; Steroids/metabolism
Chemical Substances	Hormones ; RNA (63231-63-0) ; Steroids
Language	English
Publishing date	2023-10-25
Publishing country	England
Document type	Journal Article
ZDB-ID	242586-5
ISSN	1525-3171 ; 0032-5791
ISSN (online)	1525-3171
ISSN	0032-5791
DOI	10.1016/j.psj.2023.103241
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Article ; Online: miRNA-29-3p targets PTEN to regulate follicular development through the PI3K/Akt/mTOR signaling pathway.

Hu, Chengfang / Zhao, Xiyu / Cui, Can / Zhang, Yao / Zhu, Qing / Yin, Huadong / Han, Shunshun

Theriogenology

2023 Volume 214, Page(s) 173–181

Abstract: Granulosa cells play a pivotal role in growth, development and ovulation of ovarian follicle. Simultaneously, autophagy and apoptosis processes are crucial determinants in the destiny of granulosa cells. Within this context, miR-29-3p, known to regulate ... ...

Abstract	Granulosa cells play a pivotal role in growth, development and ovulation of ovarian follicle. Simultaneously, autophagy and apoptosis processes are crucial determinants in the destiny of granulosa cells. Within this context, miR-29-3p, known to regulate a broad spectrum of biological processes and critical for tumor detection, prognosis, and treatment, is poised to clarify its roles in both autophagy and apoptosis. To enhance the understanding of the influence of miR-29-3p on follicular development, our study primarily delved into the realms autophagy and apoptosis. We employed a well-established chicken follicular atrophy model achieved through subcutaneous injection of tamoxifen (TMX) into hens. qPCR analysis revealed a significant decrease in the expression of miR-29-3p within the atrophic follicles. In our in vitro experiments with cultured chicken primary granulosa cells, miR-29-3p emerged as a novel microRNA capable of impeding autophagy and apoptosis when transfected with miR-29-3p mimics and inhibitors. Results from luciferase reporter assays corroborated that PTEN is a legitimate target of miR-29-3p. Unlike miR-29-3p, PTEN appeared to foster autophagy and apoptosis in chicken granulosa cells. Moreover, our findings uncovered that miR-29-3p facilitates the phosphorylation of Akt and mTOR proteins by targeting PTEN in chicken granulosa cells. In conclusion, the findings of this study suggest that miR-29-3p, through its targeting of PTEN via the Akt/mTOR signaling pathway, exerts inhibitory effects on autophagy and apoptosis. These effects may hold significant importance in the context of follicular development.
MeSH term(s)	Animals ; Female ; MicroRNAs/metabolism ; Proto-Oncogene Proteins c-akt/genetics ; Proto-Oncogene Proteins c-akt/metabolism ; Phosphatidylinositol 3-Kinases/genetics ; Phosphatidylinositol 3-Kinases/metabolism ; Chickens/genetics ; Chickens/metabolism ; Signal Transduction ; TOR Serine-Threonine Kinases/genetics ; TOR Serine-Threonine Kinases/metabolism ; PTEN Phosphohydrolase/genetics ; PTEN Phosphohydrolase/metabolism ; PTEN Phosphohydrolase/pharmacology ; Apoptosis/genetics
Chemical Substances	MicroRNAs ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; TOR Serine-Threonine Kinases (EC 2.7.11.1) ; PTEN Phosphohydrolase (EC 3.1.3.67)
Language	English
Publishing date	2023-10-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	189232-0
ISSN	1879-3231 ; 0093-691X
ISSN (online)	1879-3231
ISSN	0093-691X
DOI	10.1016/j.theriogenology.2023.10.024
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Article ; Online: MiRNA-21-5p induces chicken hepatic lipogenesis by targeting NFIB and KLF3 to suppress the PI3K/AKT signaling pathway.

Wu, Yamei / Zhao, Jing / Cui, Can / Zhang, Yao / Zhu, Qing / Han, Shunshun / Yang, Chaowu / Yin, Huadong

Journal of animal science

2024 Volume 102

Abstract: The liver plays a critical role in metabolic activity and is the body's first immune barrier, and maintaining liver health is particularly important for poultry production. MicroRNAs (miRNAs) are involved in a wide range of biological activities due to ... ...

Abstract	The liver plays a critical role in metabolic activity and is the body's first immune barrier, and maintaining liver health is particularly important for poultry production. MicroRNAs (miRNAs) are involved in a wide range of biological activities due to their capacity as posttranscriptional regulatory elements. A growing body of research indicates that miR-21-5p plays a vital role as a modulator of liver metabolism in various species. However, the effect of miR-21-5p on the chicken liver is unclear. In the current study, we discovered that the fatty liver had high levels of miR-21-5p. Then the qPCR, Western blot, flow cytometry, enzyme-linked immunosorbent assay, dual-luciferase, and immunofluorescence assays were, respectively, used to determine the impact of miR-21-5p in the chicken liver, and it turned out that miR-21-5p enhanced lipogenesis, oxidative stress, and inflammatory responses, which ultimately induced hepatocyte apoptosis. Mechanically, we verified that miR-21-5p can directly target nuclear factor I B (NFIB) and kruppel-like factor 3 (KLF3). Furthermore, our experiments revealed that the suppression of NFIB promoted apoptosis and inflammation, and the KLF3 inhibitor accelerated lipogenesis and enhanced oxidative stress. Furthermore, the cotransfection results suggest that the PI3K/AKT pathway is also involved in the process of miRNA-21-5p-mediate liver metabolism regulation. In summary, our study demonstrated that miRNA-21-5p plays a role in hepatocyte lipogenesis, oxidative stress, inflammation, and apoptosis, via targeting NFIB and KLF3 to suppress the PI3K/AKT signal pathway in chicken.
MeSH term(s)	Animals ; Proto-Oncogene Proteins c-akt/metabolism ; NFI Transcription Factors/metabolism ; Chickens/genetics ; Chickens/metabolism ; Phosphatidylinositol 3-Kinases/metabolism ; Lipogenesis/genetics ; Signal Transduction ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Liver/metabolism ; Apoptosis ; Inflammation/metabolism ; Inflammation/veterinary ; Cell Proliferation
Chemical Substances	Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; NFI Transcription Factors ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; MicroRNAs
Language	English
Publishing date	2024-04-02
Publishing country	United States
Document type	Journal Article
ZDB-ID	390959-1
ISSN	1525-3163 ; 0021-8812
ISSN (online)	1525-3163
ISSN	0021-8812
DOI	10.1093/jas/skae055
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Article ; Online: CircLRRFIP1 promotes the proliferation and differentiation of chicken skeletal muscle satellite cells by sponging the miR-15 family via activating AKT3-mTOR/p70S6K signaling pathway.

Wu, Yamei / Zhao, Jing / Zhao, Xiyu / He, Haorong / Cui, Can / Zhang, Yao / Zhu, Qing / Yin, Huadong / Han, Shunshun

Poultry science

2023 Volume 102, Issue 11, Page(s) 103050

Abstract: Skeletal muscle is important for animal meat production, regulating movements, and maintaining homeostasis. Circular RNAs (circRNAs) have been founded to play vital role in myogenesis. However, the effects of the numerous circRNAs on growth and ... ...

Abstract	Skeletal muscle is important for animal meat production, regulating movements, and maintaining homeostasis. Circular RNAs (circRNAs) have been founded to play vital role in myogenesis. However, the effects of the numerous circRNAs on growth and development of the skeletal muscle are yet to be uncovered. Herein, we identified circLRRFIP1, which is a novel circular RNA that is preferentially expressed in the skeletal muscle. To study the role of circLRRFIP1 in the skeletal muscle, the skeletal muscle satellite cells (SMSCs) was used to silenced or overexpressed circLRRFIP1. The results obtained in this study showed that circLRRFIP1 play a positive role in the proliferation and differentiation of SMSCs. The SMSCs were generated with stable knockdown and overexpression of circLRRFIP1, and the results showed that circLRRFIP1 exerts a stimulatory effect on the proliferation and differentiation of SMSCs. We further generated SMSCs with stable knockdown and overexpression of circLRRFIP1, and the results revealed that circLRRFIP1 exerts a stimulatory effect on the proliferation and differentiation of SMSCs. Mechanistically, circLRRFIP1 targets the myogenic inhibitory factor-miR-15 family to release the suppression of the miR-15 family to AKT3. The knockdown of AKT inhibits SMSC differentiation through the mTOR/p70S6K pathway. Taken together, the results obtained in this present study revealed the important role and the regulatory mechanisms of circLRRFIP1 in the development of chicken skeletal muscle. Therefore, this study provides an attractive target for molecular breeding to enhance meat production in the chicken industry.
MeSH term(s)	Animals ; Chickens/genetics ; Chickens/metabolism ; Ribosomal Protein S6 Kinases, 70-kDa/metabolism ; MicroRNAs/genetics ; MicroRNAs/metabolism ; RNA, Circular ; Satellite Cells, Skeletal Muscle/metabolism ; Signal Transduction ; Cell Differentiation/genetics ; TOR Serine-Threonine Kinases/genetics ; TOR Serine-Threonine Kinases/metabolism ; Muscle, Skeletal/metabolism ; Cell Proliferation/genetics ; Muscle Development
Chemical Substances	Ribosomal Protein S6 Kinases, 70-kDa (EC 2.7.11.1) ; MicroRNAs ; RNA, Circular ; TOR Serine-Threonine Kinases (EC 2.7.11.1)
Language	English
Publishing date	2023-08-21
Publishing country	England
Document type	Journal Article
ZDB-ID	242586-5
ISSN	1525-3171 ; 0032-5791
ISSN (online)	1525-3171
ISSN	0032-5791
DOI	10.1016/j.psj.2023.103050
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Article: Whole-genome resequencing analysis of Pengxian Yellow Chicken to identify genome-wide SNPs and signatures of selection.

Yin, Huadong / Li, Diyan / Wang, Yan / Zhu, Qing

3 Biotech

2019 Volume 9, Issue 11, Page(s) 383

Abstract: Chinese indigenous chickens have experienced strong selective pressure in genes or genomic regions controlling critical agricultural traits. To exploit the genetic features that may be useful in agriculture and are caused by artificial selection, we ... ...

Abstract	Chinese indigenous chickens have experienced strong selective pressure in genes or genomic regions controlling critical agricultural traits. To exploit the genetic features that may be useful in agriculture and are caused by artificial selection, we performed whole-genome sequencing of six Pengxian Yellow Chickens and downloaded the sequence data of five Red Jungle fowls from the NCBI. Through selective sweep analysis, we detected several regions with strong selection signals, containing 497 protein-coding genes. These genes were involved in developmental processes, metabolic processes, the response to external stimuli and other biological processes including digestion (ABCG5, ABCG8 and ADRB1), muscle development and growth (SMPD3, NELL1, and BICC1) and reduced immune function (CD86 and MTA3). Interestingly, we identified several genes with extremely strong selection signals associated with the loss of visual capability of domestic chickens relative to their wild ancestors. Amongst them, we propose that CTNND2 is involved in the evolutionary changes of domestic chickens toward reduced visual ability through the diopter system. VAT1 was also likely to contribute to these processes through its regulation of mitochondrial fusion. In summary, these data illustrate the patterns of genetic changes in Pengxian yellow chickens during domestication and provide valuable genetic resources that facilitate the utilization of chickens in agricultural production.
Language	English
Publishing date	2019-10-04
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2600522-0
ISSN	2190-5738 ; 2190-572X
ISSN (online)	2190-5738
ISSN	2190-572X
DOI	10.1007/s13205-019-1902-6
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Article: PDLIM5 Affects Chicken Skeletal Muscle Satellite Cell Proliferation and Differentiation via the p38-MAPK Pathway

He, Haorong / Yin, Huadong / Yu, Xueke / Zhang, Yao / Ma, Menggen / Li, Diyan / Zhu, Qing

Animals. 2021 Apr. 04, v. 11, no. 4

2021

Abstract: Skeletal muscle satellite cell growth and development is a complicated process driven by multiple genes. The PDZ and LIM domain 5 (PDLIM5) gene has been proven to function in C2C12 myoblast differentiation and is involved in the regulation of skeletal ... ...

Abstract	Skeletal muscle satellite cell growth and development is a complicated process driven by multiple genes. The PDZ and LIM domain 5 (PDLIM5) gene has been proven to function in C2C12 myoblast differentiation and is involved in the regulation of skeletal muscle development. The role of PDLIM5 in chicken skeletal muscle satellite cells, however, is unclear. In this study, in order to determine whether the PDLIM5 gene has a function in chicken skeletal muscle satellite cells, we examined the changes in proliferation and differentiation of chicken skeletal muscle satellite cells (SMSCs) after interfering and overexpressing PDLIM5 in cells. In addition, the molecular pathways of the PDLIM5 gene regulating SMSC proliferation and differentiation were analyzed by transcriptome sequencing. Our results show that PDLIM5 can promote the proliferation and differentiation of SMSCs; furthermore, through transcriptome sequencing, it can be found that the differential genes are enriched in the MAPK signaling pathway after knocking down PDLIM5. Finally, it was verified that PDLIM5 played an active role in the proliferation and differentiation of chicken SMSCs by activating the p38-MAPK signaling pathway. These results indicate that PDLIM5 may be involved in the growth and development of chicken skeletal muscle.
Keywords	cell growth ; cell proliferation ; chickens ; genes ; growth and development ; muscle development ; myoblasts ; skeletal muscle ; transcriptome
Language	English
Dates of publication	2021-0404
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
Note	NAL-AP-2-clean
ISSN	2076-2615
DOI	10.3390/ani11041016
Database	NAL-Catalogue (AGRICOLA)

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