LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 3 of total 3

Search options

  1. Article ; Online: Effects of lipid composition in cationic liposomes on suppression of mast cell activation.

    Inoh, Yoshikazu / Hirose, Takuya / Yokoi, Asami / Yokawa, Satoru / Furuno, Tadahide

    Chemistry and physics of lipids

    2020  Volume 231, Page(s) 104948

    Abstract: We previously showed that cationic liposomes composed of cholesteryl-3β-carboxyamidoethylene-N-hydroxyethylamine (OH-Chol) and 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE) inhibited mast cell degranulation mediated by the cross-linking of ... ...

    Abstract We previously showed that cationic liposomes composed of cholesteryl-3β-carboxyamidoethylene-N-hydroxyethylamine (OH-Chol) and 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE) inhibited mast cell degranulation mediated by the cross-linking of high-affinity IgE receptors (FcεRI). In this study, we prepared three kinds of cationic liposomes composed of OH-Chol and DOPE in different ratios (0.28, 0.60, and 0.86 of OH-Chol in mol ratio, named as L-liposome, M-liposome, and H-liposome, respectively) and investigated their effects on mast cell activation. We found that mast cell degranulation evoked with antigen was inhibited by pretreatment with cationic liposomes in the composite ratio-dependent manner of OH-Chol and that the H-liposome showed the highest inhibitory effect on degranulation among three kinds of liposomes. Store-operated Ca
    MeSH term(s) Animals ; Calcium/metabolism ; Cations/chemistry ; Cells, Cultured ; Endoplasmic Reticulum/drug effects ; Endoplasmic Reticulum/metabolism ; Lipids/chemistry ; Lipids/pharmacology ; Liposomes/chemistry ; Mast Cells/drug effects ; Mast Cells/metabolism ; Rats
    Chemical Substances Cations ; Lipids ; Liposomes ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2020-07-25
    Publishing country Ireland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 213869-4
    ISSN 1873-2941 ; 0009-3084
    ISSN (online) 1873-2941
    ISSN 0009-3084
    DOI 10.1016/j.chemphyslip.2020.104948
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 804: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article: Use of single-strand conformation polymorphism of amplified 16S rDNA for grouping of bacteria isolated from foods.

    Takahashi, Hajime / Kimura, Bon / Tanaka, Yuichiro / Mori, Mayumi / Yokoi, Asami / Fujii, Tateo

    Journal of food protection

    2008  Volume 71, Issue 4, Page(s) 839–844

    Abstract: The grouping method for isolated strains from foods using single-strand conformation polymorphism (SSCP) after PCR amplification of a portion of 16S rDNA was developed. This method was able to group the strains from various food samples based on 16S rDNA ...

    Abstract The grouping method for isolated strains from foods using single-strand conformation polymorphism (SSCP) after PCR amplification of a portion of 16S rDNA was developed. This method was able to group the strains from various food samples based on 16S rDNA sequence. As 97.8% of the isolated strains from various foods were grouped correctly, use of the PCR-SSCP method enables the prompt and labor-saving analysis of microbial population of food-derived bacterial strains. Advantages in speed and accuracy of bacterial population identification by the PCR-SSCP method have practical application for food suppliers and testing laboratories.
    MeSH term(s) Bacteria/classification ; Bacteria/isolation & purification ; Base Sequence ; DNA, Bacterial/genetics ; DNA, Ribosomal ; Food Contamination/analysis ; Food Microbiology ; Gene Amplification ; Gram-Negative Bacteria/classification ; Gram-Negative Bacteria/isolation & purification ; Gram-Positive Bacteria/classification ; Gram-Positive Bacteria/isolation & purification ; Molecular Sequence Data ; Phylogeny ; Polymerase Chain Reaction/methods ; Polymorphism, Single-Stranded Conformational
    Chemical Substances DNA, Bacterial ; DNA, Ribosomal
    Language English
    Publishing date 2008-03-10
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 243284-5
    ISSN 1944-9097 ; 0362-028X
    ISSN (online) 1944-9097
    ISSN 0362-028X
    DOI 10.4315/0362-028x-71.4.839
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 2631: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article: Development of multilocus single strand conformation polymorphism (MLSSCP) analysis of virulence genes of Listeria monocytogenes and comparison with existing DNA typing methods.

    Takahashi, Hajime / Handa-Miya, Satoko / Kimura, Bon / Sato, Miki / Yokoi, Asami / Goto, Seitaro / Watanabe, Itaru / Koda, Takashi / Hisa, Kazuo / Fujii, Tateo

    International journal of food microbiology

    2007  Volume 118, Issue 3, Page(s) 274–284

    Abstract: Development of rapid and simple typing methods is required for analyzing the distribution and contamination routes of food-borne pathogens. We established a simple typing method for Listeria monocytogenes using MLSSCP (Multilocus Single Strand ... ...

    Abstract Development of rapid and simple typing methods is required for analyzing the distribution and contamination routes of food-borne pathogens. We established a simple typing method for Listeria monocytogenes using MLSSCP (Multilocus Single Strand Conformation Polymorphism) analysis. Four virulence genes, hlyA, iap, actA and inlB were amplified by PCR, digested with endonucleases and applied to gels for SSCP. As banding patterns have been shown to reflect even a single nucleotide difference, this method has a potential discriminatory power comparable to that of sequencing analysis. The 64 strains isolated from five meat processing plants were divided into 18 groups by this MLSSCP. Additionally, clustering obtained with this method showed strong correspondence with phylogenetic lineages I and II, and was achieved with much less expenditure in time and cost than is required for other methods, such as MLST. The validity of the MLSSCP lineage classification was confirmed by PFGE, AFLP and ribotyping results. This newly developed MLSSCP method is suitable when obtaining accurate results quickly and simply is crucial.
    MeSH term(s) Cluster Analysis ; Food Contamination/analysis ; Food Microbiology ; Gene Amplification ; Genotype ; Listeria monocytogenes/classification ; Listeria monocytogenes/pathogenicity ; Meat Products/microbiology ; Phylogeny ; Polymerase Chain Reaction/methods ; Polymorphism, Single-Stranded Conformational ; Sensitivity and Specificity ; Species Specificity ; Virulence Factors/genetics
    Chemical Substances Virulence Factors
    Language English
    Publishing date 2007-09-30
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 87122-9
    ISSN 1879-3460 ; 0168-1605
    ISSN (online) 1879-3460
    ISSN 0168-1605
    DOI 10.1016/j.ijfoodmicro.2007.07.047
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 4668: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top