Article ; Online: [Improving the position specificity of
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
2023 Volume 39, Issue 8, Page(s) 3481–3493
Abstract: Diacylglycerol (DAG) is an intermediate product in lipid metabolism and plays an important physiological role in human body. It is mainly prepared by hydrolyzing lipid with lipase. However, research on the detection method of 1, 2-diacylglycerol (1, 2- ... ...
Abstract | Diacylglycerol (DAG) is an intermediate product in lipid metabolism and plays an important physiological role in human body. It is mainly prepared by hydrolyzing lipid with lipase. However, research on the detection method of 1, 2-diacylglycerol (1, 2-DAG) and 1, 3-diacylglycerol (1, 3-DAG) and catalytic specificity of lipase was not enough, which limits its wide application. To address these challenges, an efficient quantitative detection method was first established for 1, 2-DAG (0.025-0.200 g/L) and 1, 3-DAG (0.025-0.150 g/L) by combining supercritical fluid chromatography with evaporative light scattering detector and optimizing the detection and analysis parameters. Based on the molecular docking between |
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MeSH term(s) | Humans ; Diglycerides ; Molecular Docking Simulation ; Binding Sites ; Catalysis ; Lipase/genetics |
Chemical Substances | Diglycerides ; Lipase (EC 3.1.1.3) |
Language | Chinese |
Publishing date | 2023-08-25 |
Publishing country | China |
Document type | English Abstract ; Journal Article |
ZDB-ID | 1042206-7 |
ISSN | 1872-2075 ; 1042-749X |
ISSN (online) | 1872-2075 |
ISSN | 1042-749X |
DOI | 10.13345/j.cjb.220997 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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