LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 37

Search options

  1. Article ; Online: [Improving the position specificity of

    Ma, Yadi / You, Cuiping / Zhang, Guoqiang / Li, Jianghua / DU, Guocheng

    Sheng wu gong cheng xue bao = Chinese journal of biotechnology

    2023  Volume 39, Issue 8, Page(s) 3481–3493

    Abstract: Diacylglycerol (DAG) is an intermediate product in lipid metabolism and plays an important physiological role in human body. It is mainly prepared by hydrolyzing lipid with lipase. However, research on the detection method of 1, 2-diacylglycerol (1, 2- ... ...

    Abstract Diacylglycerol (DAG) is an intermediate product in lipid metabolism and plays an important physiological role in human body. It is mainly prepared by hydrolyzing lipid with lipase. However, research on the detection method of 1, 2-diacylglycerol (1, 2-DAG) and 1, 3-diacylglycerol (1, 3-DAG) and catalytic specificity of lipase was not enough, which limits its wide application. To address these challenges, an efficient quantitative detection method was first established for 1, 2-DAG (0.025-0.200 g/L) and 1, 3-DAG (0.025-0.150 g/L) by combining supercritical fluid chromatography with evaporative light scattering detector and optimizing the detection and analysis parameters. Based on the molecular docking between
    MeSH term(s) Humans ; Diglycerides ; Molecular Docking Simulation ; Binding Sites ; Catalysis ; Lipase/genetics
    Chemical Substances Diglycerides ; Lipase (EC 3.1.1.3)
    Language Chinese
    Publishing date 2023-08-25
    Publishing country China
    Document type English Abstract ; Journal Article
    ZDB-ID 1042206-7
    ISSN 1872-2075 ; 1042-749X
    ISSN (online) 1872-2075
    ISSN 1042-749X
    DOI 10.13345/j.cjb.220997
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  2. Article: Exploring the anti-glioma mechanism of the active components of Cortex Periplocae based on network pharmacology and iTRAQ proteomics in vitro.

    Huan, Jing / You, Cui-Ping / Lu, Yu-Cheng / Che, Feng-Yuan

    Advances in clinical and experimental medicine : official organ Wroclaw Medical University

    2023  Volume 32, Issue 11, Page(s) 1279–1290

    Abstract: Background: The active components of Cortex Periplocae (CP) exert antitumor properties in many cancers. However, little is known about their effects on glioma or the related underlying mechanisms.: Objectives: The study investigated the underlying ... ...

    Abstract Background: The active components of Cortex Periplocae (CP) exert antitumor properties in many cancers. However, little is known about their effects on glioma or the related underlying mechanisms.
    Objectives: The study investigated the underlying mechanism of CP in treating glioma.
    Material and methods: The U251 and TG905 cells were treated with an ethanol extract from CP. Cell proliferation was detected using Cell Counting Kit-8 (CCK-8) and a colony formation assay. The flow cytometric analysis was applied to explore the induction of cell cycle arrest and apoptosis. The expression levels of cell cycleand apoptosis-associated proteins were measured with western blot. A network pharmacology method was performed to predict the potential mechanism underlying the effects of the active components of CP on glioma. Then, isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics analysis was used to verify the differentially expressed proteins and pathways in order to reveal the underlying mechanisms. Furthermore, to determine the iTRAQ results, 6 candidate proteins were chosen for quantification using parallel reaction monitoring (PRM).
    Results: The CP extract inhibited the proliferation of U251 and TG905 cells and induced cell cycle arrest and apoptosis. There are 16 active compounds of CP. The antitumor mechanism of CP may be related to the apoptosis pathway, p53 signaling pathway, PI3K-AKT pathway, or transcriptional misregulation in cancer pathway. Six proteins (HSP90AB1, TOP2A, ATP1A1, TGFβ1, ATP1B1, and TYMS) were determined to be key factors involved in regulating CP in glioma.
    Conclusions: Our research revealed the underlying mechanism of CP in treating glioma using integrated network pharmacology and iTRAQ-based quantitative proteomics technology.
    MeSH term(s) Humans ; Phosphatidylinositol 3-Kinases ; Proteomics ; Network Pharmacology ; Cell Line, Tumor ; Glioma/drug therapy ; Glioma/pathology ; Apoptosis ; Cell Proliferation ; Proto-Oncogene Proteins c-akt/metabolism
    Chemical Substances Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1)
    Language English
    Publishing date 2023-11-29
    Publishing country Poland
    Document type Journal Article
    ZDB-ID 2270257-X
    ISSN 1899-5276 ; 1230-025X
    ISSN 1899-5276 ; 1230-025X
    DOI 10.17219/acem/161724
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  3. Article ; Online: Engineering hyperthermophilic pullulanase to efficiently utilize corn starch for production of maltooligosaccharides and glucose.

    Xie, Ting / Zhou, Li / Han, Laichuang / You, Cuiping / Liu, Zhongmei / Cui, Wenjing / Cheng, Zhongyi / Guo, Junling / Zhou, Zhemin

    Food chemistry

    2024  Volume 446, Page(s) 138652

    Abstract: Pullulanase is a starch-debranching enzyme that hydrolyzes side chain of starch, oligosaccharides and pullulan. Nevertheless, the limited activities of pullulanases constrain their practical application. Herein, the hyperthermophilic type II pullulanase ... ...

    Abstract Pullulanase is a starch-debranching enzyme that hydrolyzes side chain of starch, oligosaccharides and pullulan. Nevertheless, the limited activities of pullulanases constrain their practical application. Herein, the hyperthermophilic type II pullulanase from Pyrococcus yayanosii CH1 (Pul
    MeSH term(s) Starch/chemistry ; Glucose ; Zea mays/metabolism ; Glycoside Hydrolases/metabolism ; Oligosaccharides/chemistry ; Archaea ; Substrate Specificity
    Chemical Substances Starch (9005-25-8) ; pullulanase (EC 3.2.1.41) ; maltooligosaccharides ; Glucose (IY9XDZ35W2) ; Glycoside Hydrolases (EC 3.2.1.-) ; Oligosaccharides
    Language English
    Publishing date 2024-02-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 243123-3
    ISSN 1873-7072 ; 0308-8146
    ISSN (online) 1873-7072
    ISSN 0308-8146
    DOI 10.1016/j.foodchem.2024.138652
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  4. Article ; Online: Computer-Aided Reconstruction and Application of

    Zhou, Huimin / Cai, Yongchao / Long, Mengfei / Zheng, Nan / Zhang, Zehua / You, Cuiping / Hussain, Asif / Xia, Xiaole

    Journal of agricultural and food chemistry

    2024  Volume 72, Issue 2, Page(s) 1213–1227

    Abstract: β-1,4-Endoxylanase is the most critical hydrolase for xylan degradation during lignocellulosic biomass utilization. However, its poor stability and activity in hot and alkaline environments hinder its widespread application. In this study, BhS7Xyl ... ...

    Abstract β-1,4-Endoxylanase is the most critical hydrolase for xylan degradation during lignocellulosic biomass utilization. However, its poor stability and activity in hot and alkaline environments hinder its widespread application. In this study, BhS7Xyl from
    MeSH term(s) Hot Temperature ; Alkalies ; Bacillus ; Endo-1,4-beta Xylanases/genetics ; Endo-1,4-beta Xylanases/metabolism ; Hydrolysis ; Enzyme Stability ; Hydrogen-Ion Concentration
    Chemical Substances Alkalies ; Endo-1,4-beta Xylanases (EC 3.2.1.8)
    Language English
    Publishing date 2024-01-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.3c08221
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  5. Article ; Online: Simultaneous removal of tetracycline and sulfamethoxazole by laccase-mediated oxidation and ferrate(VI) oxidation: the impact of mediators and metal ions

    Tian, Qiaopeng / Zhang, Yong / Meng, Di / Zhai, Lixin / Shen, Yu / You, Cuiping / Guan, Zhengbing / Liao, Xiangru

    Environ Sci Pollut Res. 2023 Feb., v. 30, no. 6 p.15708-15721

    2023  

    Abstract: This study explores the impact of mediators and metal ions of laccase-mediated oxidation and ferrate(VI) oxidation for the simultaneous removal of tetracycline antibiotics (TCs) and sulfonamide antibiotics (SAs) and to effectively remove their ... ...

    Abstract This study explores the impact of mediators and metal ions of laccase-mediated oxidation and ferrate(VI) oxidation for the simultaneous removal of tetracycline antibiotics (TCs) and sulfonamide antibiotics (SAs) and to effectively remove their antimicrobial activity. The results showed that the antimicrobial activity of tetracycline against Bacillus altitudinis and Escherichia coli was significantly reduced, and the antimicrobial activity of sulfamethoxazole against B. altitudinis disappeared completely after treatment with the laccase–ABTS system. The combination of 6.0 U/mL of laccase and 0.2 mmol/L of ABTS removed 100% of 20.0 mg/L of tetracycline after 1.0 min at pH 6.0 and 25.0 °C, whereas the removal ratio of 20.0 mg/L of sulfamethoxazole was only 6.7%. The Al³⁺ and Cu²⁺ ions promoted the oxidation, and the Mn²⁺ ion decelerated the oxidation of tetracycline and sulfamethoxazole by the laccase-mediator systems. In contrast, the antimicrobial activity of tetracycline against B. altitudinis and E. coli was shown to be significantly reduced, and the sulfamethoxazole still retained high antimicrobial activity against B. altitudinis after treatment with Fe(VI) oxidation. The removal ratio of 20.0 mg/L of tetracycline was 100% after 1.0 min of treatment with 982.0 mg/L of K₂FeO₄ at pH 6.0 and 25.0 °C, whereas the removal ratio of 20.0 mg/L of sulfamethoxazole was only 49.5%. The Al³⁺, Cu²⁺, and Mn²⁺ ions both decelerated the oxidation of tetracycline and sulfamethoxazole by Fe(VI) oxidation. In general, the combination of the laccase–ABTS system and Fe(VI) was proposed for the simultaneous treatment of TCs and SAs in wastewater and to effectively remove their antimicrobial activity.
    Keywords Bacillus altitudinis ; Escherichia coli ; antimicrobial properties ; laccase ; oxidation ; pH ; sulfamethoxazole ; tetracycline ; wastewater
    Language English
    Dates of publication 2023-02
    Size p. 15708-15721.
    Publishing place Springer Berlin Heidelberg
    Document type Article ; Online
    ZDB-ID 1178791-0
    ISSN 1614-7499 ; 0944-1344
    ISSN (online) 1614-7499
    ISSN 0944-1344
    DOI 10.1007/s11356-022-23232-0
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

  6. Article ; Online: Development of an auto-inducible expression system by nitrogen sources switching based on the nitrogen catabolite repression regulation.

    Yan, Qin / Han, Laichuang / Liu, Xinyue / You, Cuiping / Zhou, Shengmin / Zhou, Zhemin

    Microbial cell factories

    2022  Volume 21, Issue 1, Page(s) 73

    Abstract: Background: The construction of protein expression systems is mainly focused on carbon catabolite repression and quorum-sensing systems. However, each of these regulatory modes has an inherent flaw, which is difficult to overcome. Organisms also ... ...

    Abstract Background: The construction of protein expression systems is mainly focused on carbon catabolite repression and quorum-sensing systems. However, each of these regulatory modes has an inherent flaw, which is difficult to overcome. Organisms also prioritize using different nitrogen sources, which is called nitrogen catabolite repression. To date, few gene regulatory systems based on nitrogen catabolite repression have been reported.
    Results: In this study, we constructed a nitrogen switching auto-inducible expression system (NSAES) based on nitrogen catabolite regulation and nitrogen utilization in Aspergillus nidulans. The P
    Conclusions: We described an efficient auto-inducible protein expression system based on nitrogen catabolite regulation. The system could be useful for protein production in the laboratory and industrial applications. Simultaneously, NSAES provides a new auto-inducible expression regulation mode for other filamentous fungi.
    MeSH term(s) Ammonium Compounds/metabolism ; Catabolite Repression ; Glucuronidase ; Nitrates/metabolism ; Nitrogen/metabolism
    Chemical Substances Ammonium Compounds ; Nitrates ; Glucuronidase (EC 3.2.1.31) ; Nitrogen (N762921K75)
    Language English
    Publishing date 2022-04-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 2091377-1
    ISSN 1475-2859 ; 1475-2859
    ISSN (online) 1475-2859
    ISSN 1475-2859
    DOI 10.1186/s12934-022-01794-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  7. Article ; Online: Simultaneous removal of tetracycline and sulfamethoxazole by laccase-mediated oxidation and ferrate(VI) oxidation: the impact of mediators and metal ions.

    Tian, Qiaopeng / Zhang, Yong / Meng, Di / Zhai, Lixin / Shen, Yu / You, Cuiping / Guan, Zhengbing / Liao, Xiangru

    Environmental science and pollution research international

    2022  Volume 30, Issue 6, Page(s) 15708–15721

    Abstract: This study explores the impact of mediators and metal ions of laccase-mediated oxidation and ferrate(VI) oxidation for the simultaneous removal of tetracycline antibiotics (TCs) and sulfonamide antibiotics (SAs) and to effectively remove their ... ...

    Abstract This study explores the impact of mediators and metal ions of laccase-mediated oxidation and ferrate(VI) oxidation for the simultaneous removal of tetracycline antibiotics (TCs) and sulfonamide antibiotics (SAs) and to effectively remove their antimicrobial activity. The results showed that the antimicrobial activity of tetracycline against Bacillus altitudinis and Escherichia coli was significantly reduced, and the antimicrobial activity of sulfamethoxazole against B. altitudinis disappeared completely after treatment with the laccase-ABTS system. The combination of 6.0 U/mL of laccase and 0.2 mmol/L of ABTS removed 100% of 20.0 mg/L of tetracycline after 1.0 min at pH 6.0 and 25.0 °C, whereas the removal ratio of 20.0 mg/L of sulfamethoxazole was only 6.7%. The Al
    MeSH term(s) Laccase/metabolism ; Sulfamethoxazole ; Escherichia coli/metabolism ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/chemistry ; Metals ; Tetracycline ; Oxidation-Reduction ; Ions ; Water Pollutants, Chemical/chemistry
    Chemical Substances ferrate ion (16836-06-9) ; Laccase (EC 1.10.3.2) ; Sulfamethoxazole (JE42381TNV) ; 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid (28752-68-3) ; Anti-Bacterial Agents ; Metals ; Tetracycline (F8VB5M810T) ; Ions ; Water Pollutants, Chemical
    Language English
    Publishing date 2022-09-29
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1178791-0
    ISSN 1614-7499 ; 0944-1344
    ISSN (online) 1614-7499
    ISSN 0944-1344
    DOI 10.1007/s11356-022-23232-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  8. Article: Stimulating mechanism of corn oil on biomass and polysaccharide production of Pleurotus tuber-regium mycelium

    Hu, Yan-Hong / Ge, Meng-Die / Sun, Dan-Ni / Chen, Lei / Cheung, Peter C.K. / You, Cui-Ping / Chen, Xian-Zhong / Sun, Ming-Xue / Ng, Huisuan

    International journal of biological macromolecules. 2022 Mar. 15, v. 201

    2022  

    Abstract: Hyperbranched polysaccharides (HBPSs) are the main components in cell wall and exopolysaccharide (EPS) of Pleurotus tuber-regium. To enhance the yield of these macromolecules, corn oil at 4% addition exhibited the best effect for production of mycelial ... ...

    Abstract Hyperbranched polysaccharides (HBPSs) are the main components in cell wall and exopolysaccharide (EPS) of Pleurotus tuber-regium. To enhance the yield of these macromolecules, corn oil at 4% addition exhibited the best effect for production of mycelial biomass at 20.49 g/L and EPS at 0.59 g/L, which was 2.56 folds and 1.90 folds of the control, respectively. The treated hyphae were much thicker with smooth surface, while its cell wall content (43.81 ± 0.02%) was 1.96 times of the control (22.34 ± 0.01%). Moreover, a large number of lipid droplets could be visualized under the view of confocal laser scanning microscopy (CLSM). RNA-seq analysis revealed that corn oil could enter the cells and result in the up-regulation of genes on cell morphology and membrane permeability, as well as the down-regulation on expression level of polysaccharide hydrolase and genes involved in the MAPK pathway, all of which probably contribute to the increase of polysaccharides production.
    Keywords Pleurotus tuber-regium ; biomass ; cell walls ; corn oil ; exopolysaccharides ; hydrolases ; hyphae ; lipids ; membrane permeability ; mycelium ; sequence analysis
    Language English
    Dates of publication 2022-0315
    Size p. 93-103.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2021.12.149
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

  9. Article ; Online: A novel composition of two heterozygous GFM1 mutations in a Chinese child with epilepsy and mental retardation.

    You, Cuiping / Xu, Na / Qiu, Shiyan / Li, Yufen / Xu, Liyun / Li, Xia / Yang, Li

    Brain and behavior

    2020  Volume 10, Issue 10, Page(s) e01791

    Abstract: Introduction: G elongation factor mitochondrial 1 (GFM1) encodes one of the mitochondrial translation elongation factors. GFM1 variants were reported to be associated with neurological diseases and liver diseases in a few cases. Here, we present a novel ...

    Abstract Introduction: G elongation factor mitochondrial 1 (GFM1) encodes one of the mitochondrial translation elongation factors. GFM1 variants were reported to be associated with neurological diseases and liver diseases in a few cases. Here, we present a novel composition of two heterozygous mutations of GFM1 in a boy with epilepsy, mental retardation, and other unusual phenotypes.
    Methods: The patient was found to be blind and experienced recurrent convulsive seizures such as nodding and hugging at the age of 3 months. After antiepileptic treatment with topiramate, he had no obvious seizures but still had mental retardation. The patient vomited frequently at 16 months old, sometimes accompanied by epileptic seizures. Hematuria metabolic screening, mutation screening of mitochondrial gene, and mitochondrial nuclear gene were negative. Then, he was analyzed by whole-exome sequencing (WES).
    Results: Whole-exome sequencing revealed a novel composition of two heterozygous mutations in GFM1, the maternal c.679G > A (has not been reported) and the paternal c.1765-1_1765-2del (previously reported). At present, there is no specific and effective treatment for the disease, and the prognosis is very poor.
    Conclusion: The discovery of new phenotypes and new genotypes will further enrich the diagnosis information of the disease and provide more experiences for clinicians to quickly diagnose the disease and judge the prognosis.
    MeSH term(s) Child ; China ; Epilepsy/genetics ; Heterozygote ; Humans ; Infant ; Intellectual Disability/genetics ; Male ; Mitochondrial Proteins ; Mutation ; Peptide Elongation Factor G/genetics
    Chemical Substances GFM1 protein, human ; Mitochondrial Proteins ; Peptide Elongation Factor G
    Language English
    Publishing date 2020-08-09
    Publishing country United States
    Document type Case Reports ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2623587-0
    ISSN 2162-3279 ; 2162-3279
    ISSN (online) 2162-3279
    ISSN 2162-3279
    DOI 10.1002/brb3.1791
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  10. Article: Proteomic landscape of liver tissue in old male mice that are long-term treated with polysaccharides from Sargassum fusiforme

    Chen, Peichao / Zhang, Ya / Xu, Man / Chen, Hongjun / Zou, Huixi / Zhang, Xu / Tong, Haibin / You, Cuiping / Wu, Mingjiang

    Food & function. 2020 Apr. 30, v. 11, no. 4

    2020  

    Abstract: Sargassum fusiforme is a type of brown algae and well known as a longevity promoting vegetable in Northeastern Asia. The polysaccharides derived from Sargassum fusiforme (SFPs) have been suggested as an antioxidant component for anti-aging function. ... ...

    Abstract Sargassum fusiforme is a type of brown algae and well known as a longevity promoting vegetable in Northeastern Asia. The polysaccharides derived from Sargassum fusiforme (SFPs) have been suggested as an antioxidant component for anti-aging function. However, global molecular changes in vivo by SFPs have not been fully elucidated. Here, we present a proteomics study using liver tissues of aged male mice that were fed with SFPs. Of forty-nine protein spots, thirty-eight were up-regulated and eleven were down-regulated, showing significant changes in abundance by two-dimensional gel electrophoresis. These differentially expressed proteins were mainly involved in oxidation–reduction, amino acid metabolism, and energy metabolism. Forty-six proteins were integrated into a unified network, with catalase (Cat) at the center. Intriguingly, most of the proteins were speculated as mitochondrial-located proteins. Our findings suggested that SFPs modulated antioxidant enzymes to scavenge redundant free radicals, thus preventing oxidative damage. In conclusion, our study provides a proteomic view on how SFPs have beneficial effects on the aspects of antioxidant and energy metabolism during the aging process. This study facilitates the understanding of anti-aging molecular mechanisms in polysaccharides derived from Sargassum fusiforme.
    Keywords Sargassum fusiforme ; amino acid metabolism ; antioxidant enzymes ; catalase ; energy metabolism ; free radicals ; liver ; longevity ; males ; mice ; polysaccharides ; protein synthesis ; proteins ; proteomics ; tissues ; two-dimensional gel electrophoresis ; vegetables ; Asia
    Language English
    Dates of publication 2020-0430
    Size p. 3632-3644.
    Publishing place The Royal Society of Chemistry
    Document type Article
    Note NAL-light
    ZDB-ID 2612033-1
    ISSN 2042-650X ; 2042-6496
    ISSN (online) 2042-650X
    ISSN 2042-6496
    DOI 10.1039/d0fo00187b
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

To top