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Article ; Online: Effect of miR-9 on myocardial fibrosis in rats via TGF-β1/Smads signaling pathway.

Jin, X / Yu, L-L / Yu, C-X

European review for medical and pharmacological sciences

2019 Volume 23, Issue 16, Page(s) 7083–7088

Abstract: Objective: To observe the effect of micro ribonucleic acid (miR)-9 on the myocardial fibrosis (MF) in rats and explore its mechanism of action.: Materials and methods: A total of 30 rats were selected to prepare the models of MF and randomly divided ... ...

Abstract	Objective: To observe the effect of micro ribonucleic acid (miR)-9 on the myocardial fibrosis (MF) in rats and explore its mechanism of action. Materials and methods: A total of 30 rats were selected to prepare the models of MF and randomly divided into Sham group, Model group and MiR-9 group. The rat heart weight (HW)/body weight (BW) and left ventricular weight (LVW)/BW were determined. The content of hydroxyproline (Hyp) in the myocardial tissues of left ventricle was measured using a spectrophotometer. Enzyme-linked immunosorbent assay (ELISA) was performed to determine the levels of type I and type III collagens in myocardial tissues, and the level of miR-9 in myocardial tissues was determined via reverse transcription-polymerase chain reaction (RT-PCR). Finally, Western blotting was conducted to measure the levels of transforming growth factor-β1 (TGF-β1) and Smads2 proteins. Results: Compared with Sham group, Model group exhibited increased HW/BW and LVW/BW and markedly raised Hyp content and type I collagen, type III collagen, TGF-β1 protein and Smads2 protein levels, but significantly declined miR-9 expression levels in rats. In MiR-9 group, the rats had substantially decreased HW/BW, LVW/BW, Hyp content, and type I and type III collagen levels as well as lowered TGF-β1 protein and Smads2 protein levels. Conclusions: MiR-9 can alleviate the MF in rats by inhibiting the TGF-β1/Smads signaling pathway.
MeSH term(s)	Animals ; Cardiomyopathies/metabolism ; Cardiomyopathies/pathology ; Disease Models, Animal ; Male ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Myocardium/metabolism ; Myocardium/pathology ; Rats ; Rats, Sprague-Dawley ; Signal Transduction ; Smad2 Protein/metabolism ; Transforming Growth Factor beta1/metabolism
Chemical Substances	MIRN9 microRNA, rat ; MicroRNAs ; Smad2 Protein ; Smad2 protein, rat ; Tgfb1 protein, rat ; Transforming Growth Factor beta1
Language	English
Publishing date	2019-09-05
Publishing country	Italy
Document type	Journal Article
ZDB-ID	605550-3
ISSN	2284-0729 ; 1128-3602 ; 0392-291X
ISSN (online)	2284-0729
ISSN	1128-3602 ; 0392-291X
DOI	10.26355/eurrev_201908_18752
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Article: [Argon plasma coagulation combined with CO(2) cryotherapy under bronchoscope for treatment of 40 patients with pulmoconiosis complicated by lung cancer in central airway].

Chen, L / Zhang, Y Y / Yu, C X

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases

2017 Volume 35, Issue 5, Page(s) 375–377

Language	Chinese
Publishing date	2017-08-05
Publishing country	China
Document type	Journal Article
ISSN	1001-9391
ISSN	1001-9391
DOI	10.3760/cma.j.issn.1001-9391.2017.05.016
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Article ; Online: Free fatty acids mediates human umbilical vein endothelial cells inflammation through toll-like receptor-4.

Chen, L / Yu, C-X / Song, B / Cai, W / Liu, C / Guan, Q-B

European review for medical and pharmacological sciences

2018 Volume 22, Issue 8, Page(s) 2421–2431

Abstract: Objective: To investigate the role of Toll-like receptor-4 (TLR4) in the free fatty acids (FFAs) induced human umbilical vein endothelial cells (HUVECs) inflammation and to explore the underlying mechanisms.: Materials and methods: HUVECs and HEK293 ... ...

Abstract	Objective: To investigate the role of Toll-like receptor-4 (TLR4) in the free fatty acids (FFAs) induced human umbilical vein endothelial cells (HUVECs) inflammation and to explore the underlying mechanisms. Materials and methods: HUVECs and HEK293 cell lines were obtained from Shanghai Type Culture Collection. Cell counting kit-8 (CCK8) and flow cytometry (FCM) were performed to examine the cell viability and apoptosis rate of HUVECs induced by FFAs treatments with or without infection of toll-like receptor-4 interference (TLR4i) adenovirus. Enzyme-linked immunosorbent assay (ELISA) was performed to evaluate the inflammatory cytokines release. Quantitative polymerase chain reaction (qPCR) and Western Blot (WB) were used to test the molecular mechanisms of inflammation. Results: FFAs induced inflammatory responses in HUVECs via modulating the TLR4 receptor complex. TLR4i adenovirus interference increased cell viability and decreased cell apoptosis rate. FFAs treatments significantly increased the expressions of inflammatory cytokines interleukin-6 (IL-6), interleukin-8 (IL-8), C-C motif chemokine ligand 5 (CCL5) and CXC chemokine ligand 10 (CXCL10), while TLR4i adenovirus interference significantly reduced these cytokines levels. TLR4-mediated myeloid differential protein-88 (MyD88) expression activating the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and inhabiting kappa B kinase-beta (IKK-β). TLR4i adenovirus interference decreased the expressions of these genes at both mRNA level and protein level. Conclusions: TLR4 mediates FFAs induced inflammatory responses in HUVECs. TLR4 interference in HUVECs significantly reduces the inflammatory cytokines expression, decreases the cell apoptosis rate and increases cell viability.
MeSH term(s)	Cell Survival/drug effects ; Cell Survival/physiology ; Fatty Acids, Nonesterified/toxicity ; HEK293 Cells ; Human Umbilical Vein Endothelial Cells/drug effects ; Human Umbilical Vein Endothelial Cells/metabolism ; Humans ; Inflammation/chemically induced ; Inflammation/metabolism ; Inflammation Mediators/antagonists & inhibitors ; Inflammation Mediators/metabolism ; Toll-Like Receptor 4/antagonists & inhibitors ; Toll-Like Receptor 4/metabolism ; Umbilical Veins/drug effects ; Umbilical Veins/metabolism
Chemical Substances	Fatty Acids, Nonesterified ; Inflammation Mediators ; TLR4 protein, human ; Toll-Like Receptor 4
Language	English
Publishing date	2018-05-15
Publishing country	Italy
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605550-3
ISSN	2284-0729 ; 1128-3602 ; 0392-291X
ISSN (online)	2284-0729
ISSN	1128-3602 ; 0392-291X
DOI	10.26355/eurrev_201804_14835
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Article: Design considerations for the sides of multileaf collimator leaves.

Yu, C X

Physics in medicine and biology

1998 Volume 43, Issue 5, Page(s) 1335–1342

Abstract: The use of multileaf collimators (MLCs) in radiation therapy is becoming increasingly widespread as more commercial vendors offer them as options on medical linear accelerators. In most of the commercially available multileaf collimators, the sides of ... ...

Abstract	The use of multileaf collimators (MLCs) in radiation therapy is becoming increasingly widespread as more commercial vendors offer them as options on medical linear accelerators. In most of the commercially available multileaf collimators, the sides of the leaves have a 'tongue and groove' (TG) feature, which is necessary for blocking the straight through leakage when the leaves are staggered together. The dosimetric effects of the TG design have not been fully studied for applications in block replacement or in dynamic intensity modulation. This article analytically explains the TG effect by calculating a deficit in photon flux when the side of a leaf is exposed. It is shown that the maximum photon deficit occurs when the leaf thickness is shared equally by the tongue and groove of adjacent leaves. New designs are proposed based on the theoretical analysis and improvements of the new design in leakage and the TG effect are verified with Monte-Carlo simulations. Measurements of the TG effect on an MLC from one manufacturer are also conducted. The results of the measurements confirm the analytical explanations of the TG effect.
MeSH term(s)	Computer Simulation ; Equipment Design ; Monte Carlo Method ; Particle Accelerators ; Radiotherapy/instrumentation ; Radiotherapy Dosage ; Radiotherapy Planning, Computer-Assisted
Language	English
Publishing date	1998-05
Publishing country	England
Document type	Journal Article
ZDB-ID	208857-5
ISSN	1361-6560 ; 0031-9155
ISSN (online)	1361-6560
ISSN	0031-9155
DOI	10.1088/0031-9155/43/5/022
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Article ; Online: Necrostatin-1 Ameliorates Neutrophilic Inflammation in Asthma by Suppressing MLKL Phosphorylation to Inhibiting NETs Release.

Han, X A / Jie, H Y / Wang, J H / Zhang, X M / Wang, Jun / Yu, C X / Zhang, J L / He, J / Chen, J Q / Lai, K F / Sun, E W

Frontiers in immunology

2020 Volume 11, Page(s) 666

Abstract: Neutrophilic inflammation occurs during asthma exacerbation, and especially, in patients with steroid-refractory asthma, but the underlying mechanisms are poorly understood. Recently, a significant accumulation of neutrophil extracellular traps (NETs) in ...

Abstract	Neutrophilic inflammation occurs during asthma exacerbation, and especially, in patients with steroid-refractory asthma, but the underlying mechanisms are poorly understood. Recently, a significant accumulation of neutrophil extracellular traps (NETs) in the airways of neutrophilic asthma has been documented, suggesting that NETs may play an important role in the pathogenesis. In this study, we firstly demonstrated that NETs could induce human airway epithelial cell damage
MeSH term(s)	Animals ; Anti-Asthmatic Agents/therapeutic use ; Asthma/drug therapy ; Disease Models, Animal ; Extracellular Traps/metabolism ; Humans ; Imidazoles/therapeutic use ; Indoles/therapeutic use ; Inflammation/drug therapy ; Male ; Mice ; Mice, Inbred C57BL ; Neutrophil Activation ; Neutrophils/immunology ; Phosphorylation ; Protein Kinases/metabolism
Chemical Substances	Anti-Asthmatic Agents ; Imidazoles ; Indoles ; necrostatin-1 ; MLKL protein, human (EC 2.7.-) ; Protein Kinases (EC 2.7.-)
Language	English
Publishing date	2020-04-24
Publishing country	Switzerland
Document type	Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2020.00666
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Article: First Report of Cladobotryum protrusum Causing Cobweb Disease on Cultivated Morchella importuna

Lan, Y. F / An, X. R / Cong, Q. Q / Cui, X / Kong, F. H / Li, X. D / Li, X. M / Tang, L. N / Wang, Q. W / Yu, C. X / Yu, Q. W

Plant disease. 2020 Mar., v. 104, no. 3

2020

Abstract: Morel mushroom (Morchella spp.) has high nutritional, medicinal, and commercial values and is popular worldwide. The cultivation area of M. importuna has been increasing rapidly in China since 2012. Now, China has become the largest producer and exporter ...

Abstract	Morel mushroom (Morchella spp.) has high nutritional, medicinal, and commercial values and is popular worldwide. The cultivation area of M. importuna has been increasing rapidly in China since 2012. Now, China has become the largest producer and exporter of morel mushrooms. During a survey in Tai’an of Shandong Province, China, in April 2019, unusual symptoms were observed on M. importuna, with an incidence of 2 to 5% in three of the 20 mushroom sheds investigated. First, white cobweb-like mycelia appeared on the surface of the soil and the base of the stipe, spreading gradually to the pileus. Later, the fruiting bodies of M. importuna became soft. Infected tissues of M. importuna were plated onto potato dextrose agar (PDA) and incubated at 25°C in the dark to isolate the pathogen. Five fungal isolates with the same morphology were obtained from different fruiting bodies. Colonies on PDA grew fast, reaching 90 mm in 3 to 4 days at 25°C, and were white to yellowish in color before turning brick red. Conidiogenous cells were polyblastic and had protrusions on the tips. Conidia were ellipsoidal, 20.8 to 25.6 × 5.6 to 7.8 μm, hyaline to pale yellowish green, and had no or one septum. All these characteristics were consistent with those of Cladobotryum protrusum (Põldmaa 2011). To confirm the identity of the pathogen, the genomic fragments for the internal transcribed spacer (ITS), 28S rDNA, and translation elongation factor 1-alpha (TEF1) of two isolates were amplified by PCR. The resulting sequences from one isolate were deposited in GenBank (MN208482, MN208480, and MN208485, respectively). BLAST results showed >99% identity with those of C. protrusum (KU237239, MH875894, and FN868727). A pathogenicity test was performed by spraying 30 ml of spore suspension (1 × 106 conidia/ml) onto 30 young fruiting bodies and associated casing soil of M. importuna. Fruiting bodies and casing soil treated with the same volume of sterilized distilled water were used as controls. All treatments were placed in the mushroom shed (18 to 22°C, 90% relative humidity) for symptom development. Cobweb-like mycelia and soft M. importuna fruiting bodies were observed 5 to 7 days after inoculation with the spore suspension, whereas the controls remained healthy. Isolates of C. protrusum that were morphologically the same as those used for inoculation were reisolated from diseased fruiting bodies but not from the controls. Cobweb disease and its causal agent C. protrusum were previously reported on cultivated Coprinus comatus in China (Wang et al. 2015). To our knowledge, this is the first report of C. protrusum causing cobweb on cultivated M. importuna in the world. Considering that it could infect the fruiting bodies and significantly reduce the commercial value of M. importuna, C. protrusum poses a potential threat to the production of M. importuna in China.
Keywords	cobweb disease ; color ; conidia ; Coprinus comatus ; cultivation area ; culture media ; fruiting bodies ; genomics ; Hypomyces ; internal transcribed spacers ; Morchella ; mushrooms ; mycelium ; pathogenicity ; pathogens ; peptide elongation factors ; polymerase chain reaction ; relative humidity ; ribosomal DNA ; soil ; spraying ; surveys ; China
Language	English
Dates of publication	2020-03
Publishing place	Plant Disease
Document type	Article
ZDB-ID	754182-x
ISSN	0191-2917
ISSN	0191-2917
DOI	10.1094/PDIS-08-19-1611-PDN
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Bilateral superficial cervical plexus block reduces postoperative nausea and vomiting and early postoperative pain after thyroidectomy.

Cai, H-D / Lin, C-Z / Yu, C-X / Lin, X-Z

The Journal of international medical research

2012 Volume 40, Issue 4, Page(s) 1390–1398

Abstract: Objective: To compare the incidence of postoperative nausea and vomiting (PONV) and postoperative pain in thyroidectomy patients undergoing general anaesthesia, with or without bilateral superficial cervical plexus block (BSCPB).: Methods: In this ... ...

Abstract	Objective: To compare the incidence of postoperative nausea and vomiting (PONV) and postoperative pain in thyroidectomy patients undergoing general anaesthesia, with or without bilateral superficial cervical plexus block (BSCPB). Methods: In this prospective, randomized, double-blind study, adult patients scheduled for thyroid surgery under general anaesthesia were randomized to receive BSCPB with 20 ml 0.5% ropivacaine (ropivacaine group) or placebo (20 ml saline; saline group) before surgery. The incidence of PONV and postoperative pain, and the need for rescue antiemetics were assessed at 0-24 h postoperatively. Results: Data from 135 patients were evaluated and the incidence of PONV, the need for rescue antiemetics and the number of patients needing additional perioperative pain relief in the postanaesthetic care unit were significantly lower in the ropivacaine group compared with the saline group. Early postoperative (0-8 h) visual analogue scale pain scores were significantly lower in the ropivacaine group compared with the saline group. Conclusions: BSCPB with 0.5% ropivacaine administered before surgery can significantly reduce the incidence of PONV and early postoperative pain and also reduce perioperative opioid requirements in thyroidectomy patients undergoing general anaesthesia.
MeSH term(s)	Adult ; Amides ; Analgesics/administration & dosage ; Analysis of Variance ; Anesthetics, Local ; Antiemetics/administration & dosage ; Autonomic Nerve Block ; Cervical Plexus ; Double-Blind Method ; Female ; Flurbiprofen/administration & dosage ; Humans ; Male ; Middle Aged ; Ondansetron/administration & dosage ; Pain, Postoperative/drug therapy ; Pain, Postoperative/prevention & control ; Postoperative Care ; Postoperative Nausea and Vomiting/drug therapy ; Postoperative Nausea and Vomiting/prevention & control ; Prospective Studies ; Statistics, Nonparametric ; Thyroidectomy/adverse effects
Chemical Substances	Amides ; Analgesics ; Anesthetics, Local ; Antiemetics ; Ondansetron (4AF302ESOS) ; Flurbiprofen (5GRO578KLP) ; ropivacaine (7IO5LYA57N)
Language	English
Publishing date	2012
Publishing country	England
Document type	Journal Article ; Randomized Controlled Trial
ZDB-ID	184023-x
ISSN	1473-2300 ; 0300-0605 ; 0142-2596
ISSN (online)	1473-2300
ISSN	0300-0605 ; 0142-2596
DOI	10.1177/147323001204000417
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Zs.A 925: Show issues

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Article: Intensity-modulated arc therapy with dynamic multileaf collimation: an alternative to tomotherapy.

Yu, C X

Physics in medicine and biology

1995 Volume 40, Issue 9, Page(s) 1435–1449

Abstract: The desire to improve local tumour control and cure more cancer patients, coupled with advances in computer technology and linear accelerator design, has spurred the developments of three-dimensional conformal radiotherapy techniques. Optimized treatment ...

Abstract	The desire to improve local tumour control and cure more cancer patients, coupled with advances in computer technology and linear accelerator design, has spurred the developments of three-dimensional conformal radiotherapy techniques. Optimized treatment plans, aiming to deliver high dose to the target while minimizing dose to the surrounding tissues, can be delivered with multiple fields each with spatially modulated beam intensities or with multiple-slice treatments. This paper introduces a new method, intensity-modulated arc therapy (IMAT), for delivering optimized treatment plans to improve the therapeutic ratio. It utilizes continuous gantry motion as in conventional arc therapy. Unlike conventional arc therapy, the field shape, which is conformed with the multileaf collimator, changes during gantry rotation. Arbitrary two-dimensional beam intensify distributions at different beam angles are delivered with multiple superimposing arcs. A system capable of delivering the IMAT has been implemented. An example is given that illustrates the feasibility of this new method. Advantages of this new technique over tomotherapy and other slice-based delivery schemes are also discussed.
MeSH term(s)	Algorithms ; Head ; Humans ; Neoplasms/radiotherapy ; Phantoms, Imaging ; Radiotherapy/instrumentation ; Radiotherapy Dosage ; Radiotherapy Planning, Computer-Assisted
Language	English
Publishing date	1995-09
Publishing country	England
Document type	Comparative Study ; Journal Article
ZDB-ID	208857-5
ISSN	1361-6560 ; 0031-9155
ISSN (online)	1361-6560
ISSN	0031-9155
DOI	10.1088/0031-9155/40/9/004
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Article: [Gonadal tumor and Y chromosome].

Yu, C X

Zhonghua fu chan ke za zhi

1993 Volume 28, Issue 12, Page(s) 728–30, 760

Abstract: The histologic features of gonads occurrence of gonadal tumor, and RIA reproductive hormone levels in 50 cases containing a Y chromosome karyotype were analysed. The correlation between gonadal tumor and Y chromosome, clinical manifestation and genotype, ...

Abstract	The histologic features of gonads occurrence of gonadal tumor, and RIA reproductive hormone levels in 50 cases containing a Y chromosome karyotype were analysed. The correlation between gonadal tumor and Y chromosome, clinical manifestation and genotype, and the principle of diagnosis and treatment are discussed. There were seven cases of gonadal tumor in these fifty cases the frequency of gonadal tumor is 14% (7/50). All of them were present in cases with gonadal dysgenesis, with the frequency rate of 27% (7/26). Five cases were bilateral, and two were unilateral, that made a total of twelve tumors in these seven cases. Gonadoblastoma was type of tumor commonly found, about 75% (9/12). The above date suggested that there is a higher frequency of gonadal tumor in cases of gonadal dysgenesis with a Y chromosome. Both hypergonadotropins and low sex steroids levels were the feature of gonadal dysgenesis, but no relative with occurrence of the tumor. The phenotype of these fifty cases were largely due to the process of abnormal sexual differentiation not only depend on the sex chromosome complement. In treatment, prophylactic bilateral gonadectomy is emphasized.
MeSH term(s)	Female ; Gonadal Dysgenesis/complications ; Gonadal Dysgenesis, 46,XY/complications ; Gonadoblastoma/etiology ; Humans ; Male ; Ovarian Neoplasms/etiology ; Sex Chromosome Aberrations ; Testicular Neoplasms/etiology ; Turner Syndrome/complications ; Y Chromosome
Language	Chinese
Publishing date	1993-12
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	604570-4
ISSN	0529-567X
ISSN	0529-567X
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Article ; Online: Profile preferentially partial occlusion removal for three-dimensional integral imaging.

Xie, S L / Wang, P / Sang, X Z / Chen, Z D / Guo, N / Yan, B B / Wang, K R / Yu, C X

Optics express

2016 Volume 24, Issue 20, Page(s) 23519–23530

Abstract: A profile preferentially partial occlusion removal method for integral imaging is presented. The profile of the occlusion always contains details with significant texture structure, and regions with significant texture structure often lead to reliable ... ...

Abstract	A profile preferentially partial occlusion removal method for integral imaging is presented. The profile of the occlusion always contains details with significant texture structure, and regions with significant texture structure often lead to reliable depth estimation. Taking the advantage of the significant texture structure, the profile of occlusion is preferentially dealt with, and then the entire occlusion region is determined via regional spreading according to the accurate profile. The details of occlusion can be accurately removed and the occluded scene is also retained to the maximum degree. In our method, elemental images are integrated into a four-dimensional light field to provide consistently reliable depth estimation and occlusion decisions among all elemental images. Experimental results show that the proposed method is efficient to deal with the details of the occlusion, and it is robust for the occlusions with different kinds of texture structure.
Language	English
Publishing date	2016-10-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	1491859-6
ISSN	1094-4087 ; 1094-4087
ISSN (online)	1094-4087
ISSN	1094-4087
DOI	10.1364/OE.24.023519
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