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Article ; Online: Use of ultrasound to increase the catalytic activity of α-L-rhamnosidase.

Qi, Chen / Li, Le / Yu, Kunpeng / Lin, Yanling / Li, Lijun

Preparative biochemistry & biotechnology

2024 , Page(s) 1–5

Abstract: α-L-rhamnosidase (Rha) is ubiquitous in nature and has high feasibility in the food and biotechnology industries. A green and environmentally friendly method was used to improve the activity of Rha. Here, we show that the effects of ultrasound treatment ... ...

Abstract	α-L-rhamnosidase (Rha) is ubiquitous in nature and has high feasibility in the food and biotechnology industries. A green and environmentally friendly method was used to improve the activity of Rha. Here, we show that the effects of ultrasound treatment on the Rha. Ultrasonic treatment at 80 W for 10 min yielded the highest enzyme activity. Treatment increased enzyme activity by 26.3% and half-life by 124 min. Further, treatment increased the catalytic efficiency of Rha and increased the substrate conversion rate by 33.88%. These results demonstrate that ultrasound increases the catalytic activity and stability of Rha. Thus, ultrasonic treatment of Rha is cost-effective on the industrial scale.
Language	English
Publishing date	2024-03-13
Publishing country	England
Document type	Journal Article
ZDB-ID	1322522-4
ISSN	1532-2297 ; 1082-6068
ISSN (online)	1532-2297
ISSN	1082-6068
DOI	10.1080/10826068.2024.2326877
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Article ; Online: Antifouling strategies for electrochemical sensing in complex biological media.

Song, Zhen / Han, Rui / Yu, Kunpeng / Li, Rong / Luo, Xiliang

Mikrochimica acta

2024 Volume 191, Issue 3, Page(s) 138

Abstract: Surface fouling poses a significant challenge that restricts the analytical performance of electrochemical sensors in both in vitro and in vivo applications. Biofouling resistance is paramount to guarantee the reliable operation of electrochemical ... ...

Abstract	Surface fouling poses a significant challenge that restricts the analytical performance of electrochemical sensors in both in vitro and in vivo applications. Biofouling resistance is paramount to guarantee the reliable operation of electrochemical sensors in complex biofluids (e.g., blood, serum, and urine). Seeking efficient strategies for surface fouling and establishing highly sensitive sensing platforms for applications in complex media have received increasing attention in the past. In this review, we provide a comprehensive overview of recent research efforts focused on antifouling electrochemical sensors. Initially, we present a detailed illustration of the concept about biofouling along with an exploration of four key antifouling mechanisms. Subsequently, we delve into the commonly employed antifouling strategies in the fabrication of electrochemical sensors. These encompass physical surface topography (micro/nanostructure coatings and filtration membranes) and chemical surface modifications (PEG and its derivatives, zwitterionic polymers, peptides, proteins, and various other antifouling materials). The progress in antifouling electrochemical sensors is proposed concerning the antifouling mechanisms as well as sensing capability assessments (e.g., sensitivity, stability, and practical application ability). Finally, we summarize the evolving trends in the field and highlight some key remaining limitations.
MeSH term(s)	Biofouling/prevention & control ; Polymers/chemistry ; Proteins ; Peptides/chemistry ; Nanostructures/chemistry
Chemical Substances	Polymers ; Proteins ; Peptides
Language	English
Publishing date	2024-02-16
Publishing country	Austria
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	89-9
ISSN	1436-5073 ; 0026-3672
ISSN (online)	1436-5073
ISSN	0026-3672
DOI	10.1007/s00604-024-06218-2
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Article: Application status and future prospects of the PDX model in lung cancer.

Liu, Wei / Cui, Yishuang / Zheng, Xuan / Yu, Kunpeng / Sun, Guogui

Frontiers in oncology

2023 Volume 13, Page(s) 1098581

Abstract: Lung cancer is one of the most prevalent, fatal, and highly heterogeneous diseases that, seriously threaten human health. Lung cancer is primarily caused by the aberrant expression of multiple genes in the cells. Lung cancer treatment options include ... ...

Abstract	Lung cancer is one of the most prevalent, fatal, and highly heterogeneous diseases that, seriously threaten human health. Lung cancer is primarily caused by the aberrant expression of multiple genes in the cells. Lung cancer treatment options include surgery, radiation, chemotherapy, targeted therapy, and immunotherapy. In recent decades, significant progress has been made in developing therapeutic agents for lung cancer as well as a biomarker for its early diagnosis. Nonetheless, the alternative applications of traditional pre-clinical models (cell line models) for diagnosis and prognosis prediction are constrained by several factors, including the lack of microenvironment components necessary to affect cancer biology and drug response, and the differences between laboratory and clinical results. The leading reason is that substantial shifts accrued to cell biological behaviors, such as cell proliferative, metastatic, invasive, and gene expression capabilities of different cancer cells after decades of growing indefinitely
Language	English
Publishing date	2023-03-24
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2649216-7
ISSN	2234-943X
ISSN	2234-943X
DOI	10.3389/fonc.2023.1098581
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Article: Study on the fluidity of foamed alkali-activated slag cementitious material (AASCM).

Yu, Kunpeng / Ma, Liqiang / Ngo, Ichhuy / Wang, Yangyang / Zhai, Jiangtao

Heliyon

2023 Volume 9, Issue 11, Page(s) e22277

Abstract: This study aims to investigate the evolution patterns of fluidity and rheological properties of AASCM under varying dosages of foaming agent and particle sizes of filling aggregate. The flow characteristics of AASCM are significantly affected by the ... ...

Abstract	This study aims to investigate the evolution patterns of fluidity and rheological properties of AASCM under varying dosages of foaming agent and particle sizes of filling aggregate. The flow characteristics of AASCM are significantly affected by the filling aggregate's size and the foaming agent's dosage. Specifically, an increase in filling aggregate size (D(4,3) ϵ [26 μm, 69 μm]) enhances the fluidity of foamed AASCM, while an increase in foaming agent dosage reduces fluidity. These observed variations can be attributed to the presence of particle voids, the specific surface area of the aggregate, as well as the quantity and spatial distribution of bubbles within the slurry. A bubble-particle packing model is established, and by calibrating the simulation error coefficient to 1.1, the study investigates the evolution of water film thickness (WFT) in foamed AASCM with slurry expansion degree. It is observed that bubbles in the slurry affect the fluidity by altering the overall compactness and specific surface area of the foamed slurry, subsequently modifying the WFT.
Language	English
Publishing date	2023-11-14
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2023.e22277
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Article ; Online: Effect of the co-activation of sodium silicate and CO2 on setting and mechanical properties of coal gangue-fly ash backfill (CGFB)

Ngo, Ichhuy / Ma, Liqiang / Zhai, Jiangtao / Wang, Yangyang / Xu, Yujun / Wei, Tianxiang / Yu, Kunpeng

Environ Earth Sci. 2023 Apr., v. 82, no. 7 p.190-190

2023

Abstract: Static segregation of coal gangue-fly ash backfill (CGFB) material presents a significant impact on its mechanical performance for underground support. To resolve, a novel formulation was addressed using sodium silicate (SS) and CO₂ as co-activator. Its ... ...

Abstract	Static segregation of coal gangue-fly ash backfill (CGFB) material presents a significant impact on its mechanical performance for underground support. To resolve, a novel formulation was addressed using sodium silicate (SS) and CO₂ as co-activator. Its setting behaviors and mechanical properties were investigated with respect to the coal gangue content, CO₂ influx and the concentration of sodium silicate solution. The microstructure was characterized by SEM, EDX, XRD, and FTIR. The present method lowered the initial and final setting times to approximately 90% and 74% comparing to which of conventional activator. The compressive strength increased from 2.06 to 10.23 MPa with coal gangue ratio of 3.2 after 56 days curing. This mainly results from the mitigation of the effect of segregation through the generation of silica gel, which precipitated on the grain surface. The silica gel promoted the interparticle binding and rapid consistency, thus preventing gangue from settlement. Incorporating the microscale crystalline phase characterization, the carbonate products work as the filling particle and the coal gangue presents as the reinforcement after hardening, leading to the significant increase in material strength. This method not only ensures safe disposal of coal gangue and fly ash from segregation, but also mitigates overburden deformation and promotes CO₂ utilization. Therefore, the coordinated development of coal resource development, environmental protection, and carbon footprint reduction is realized.
Keywords	carbon dioxide ; carbon footprint ; carbonates ; coal ; compression strength ; deformation ; environmental protection ; fly ash ; microstructure ; resource management ; silica gel ; sodium silicate
Language	English
Dates of publication	2023-04
Size	p. 190.
Publishing place	Springer Berlin Heidelberg
Document type	Article ; Online
ZDB-ID	2493699-6
ISSN	1866-6299 ; 1866-6280
ISSN (online)	1866-6299
ISSN	1866-6280
DOI	10.1007/s12665-023-10863-w
Database	NAL-Catalogue (AGRICOLA)

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Article: The Neuromodulatory Role of the Noradrenergic and Cholinergic Systems and Their Interplay in Cognitive Functions: A Focused Review.

Slater, Cody / Liu, Yuxiang / Weiss, Evan / Yu, Kunpeng / Wang, Qi

Brain sciences

2022 Volume 12, Issue 7

Abstract: The noradrenergic and cholinergic modulation of functionally distinct regions of the brain has become one of the primary organizational principles behind understanding the contribution of each system to the diversity of neural computation in the central ... ...

Abstract	The noradrenergic and cholinergic modulation of functionally distinct regions of the brain has become one of the primary organizational principles behind understanding the contribution of each system to the diversity of neural computation in the central nervous system. Decades of work has shown that a diverse family of receptors, stratified across different brain regions, and circuit-specific afferent and efferent projections play a critical role in helping such widespread neuromodulatory systems obtain substantial heterogeneity in neural information processing. This review briefly discusses the anatomical layout of both the noradrenergic and cholinergic systems, as well as the types and distributions of relevant receptors for each system. Previous work characterizing the direct and indirect interaction between these two systems is discussed, especially in the context of higher order cognitive functions such as attention, learning, and the decision-making process. Though a substantial amount of work has been done to characterize the role of each neuromodulator, a cohesive understanding of the region-specific cooperation of these two systems is not yet fully realized. For the field to progress, new experiments will need to be conducted that capitalize on the modular subdivisions of the brain and systematically explore the role of norepinephrine and acetylcholine in each of these subunits and across the full range of receptors expressed in different cell types in these regions.
Language	English
Publishing date	2022-07-07
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2651993-8
ISSN	2076-3425
ISSN	2076-3425
DOI	10.3390/brainsci12070890
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Article ; Online: [Expression of β-glucosidase

Yu, Kunpeng / Peng, Cheng / Lin, Yanling / Li, Lijun / Ni, Hui / Li, Qingbiao

Sheng wu gong cheng xue bao = Chinese journal of biotechnology

2022 Volume 39, Issue 3, Page(s) 1232–1246

Abstract: Scopoletin is a coumarin compound with various biological activities including detumescence and analgesic, insecticidal, antibacterial and acaricidal effects. However, interference with scopolin and other components often leads to difficulties in ... ...

Abstract	Scopoletin is a coumarin compound with various biological activities including detumescence and analgesic, insecticidal, antibacterial and acaricidal effects. However, interference with scopolin and other components often leads to difficulties in purification of scopoletin with low extraction rates from plant resource. In this paper, heterologous expression of the gene encoding β-glucosidase
MeSH term(s)	Aspergillus niger/genetics ; beta-Glucosidase/genetics ; beta-Glucosidase/chemistry ; Scopoletin ; Polysorbates ; Coumarins
Chemical Substances	scopolin (1Y49270PY8) ; beta-Glucosidase (EC 3.2.1.21) ; Scopoletin (KLF1HS0SXJ) ; Polysorbates ; Coumarins
Language	Chinese
Publishing date	2022-11-14
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	1042206-7
ISSN	1872-2075 ; 1042-749X
ISSN (online)	1872-2075
ISSN	1042-749X
DOI	10.13345/j.cjb.220709
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Article ; Online: [Expression of β-xylosidase

Li, Le / Peng, Cheng / Yu, Kunpeng / Tang, Yiling / Lin, Yanling / Li, Lijun / Ni, Hui / Li, Qingbiao

Sheng wu gong cheng xue bao = Chinese journal of biotechnology

2023 Volume 39, Issue 11, Page(s) 4593–4607

Abstract: The hydrolysis of xylo-oligosaccharides catalyzed by β-xylosidase plays an important role in the degradation of lignocellulose. However, the enzyme is easily inhibited by its catalytic product xylose, which severely limits its application. Based on ... ...

Abstract	The hydrolysis of xylo-oligosaccharides catalyzed by β-xylosidase plays an important role in the degradation of lignocellulose. However, the enzyme is easily inhibited by its catalytic product xylose, which severely limits its application. Based on molecular docking, this paper studied the xylose affinity of
MeSH term(s)	Aspergillus niger/genetics ; Xylose/metabolism ; Molecular Docking Simulation ; Xylosidases/genetics ; Cellulases ; Tea ; Hydrogen-Ion Concentration ; Substrate Specificity
Chemical Substances	exo-1,4-beta-D-xylosidase (EC 3.2.1.37) ; Xylose (A1TA934AKO) ; Xylosidases (EC 3.2.1.-) ; Cellulases (EC 3.2.1.-) ; Tea
Language	Chinese
Publishing date	2023-09-15
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	1042206-7
ISSN	1872-2075 ; 1042-749X
ISSN (online)	1872-2075
ISSN	1042-749X
DOI	10.13345/j.cjb.230206
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Article ; Online: Effect of Electrolyte Chemistry and Sulfur Content in Li||Sulfurized Polyacrylonitrile (SPAN) Batteries.

Yu, Kunpeng / Cai, Guorui / Li, Mingqian / Wu, Junlin / Gupta, Varun / Lee, Dong Ju / Holoubek, John / Chen, Zheng

ACS applied materials & interfaces

2023 Volume 15, Issue 37, Page(s) 43724–43731

Abstract: Sulfurized polyacrylonitrile (SPAN) is considered as a high-value cathode material, which leverages the high energy of S redox while mitigating the negative externalities that limit elemental S cycling. As such, the sulfur content in Li-SPAN batteries ... ...

Abstract	Sulfurized polyacrylonitrile (SPAN) is considered as a high-value cathode material, which leverages the high energy of S redox while mitigating the negative externalities that limit elemental S cycling. As such, the sulfur content in Li-SPAN batteries plays a critical role. In this work, we demonstrate that high-S loading SPAN cathodes, where the PAN backbone approaches the saturation point without signs of elemental S, are highly dependent on the electrolyte chemistry for long-term reversibility. Specifically, we find that a localized-high-concentration electrolyte (LHCE) further enhances the reversible capacity and cycling stability of SPAN cathode with optimized S content relative to a carbonate control, largely due to the formation of a compatible interphase. With this LHCE as the electrolyte and 43% sulfur ratio of SPAN as the cathode, a full cell applying N/P ratio = 1.82, a cathode loading of 6 mAh cm
Language	English
Publishing date	2023-09-11
Publishing country	United States
Document type	Journal Article
ISSN	1944-8252
ISSN (online)	1944-8252
DOI	10.1021/acsami.3c08338
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Article ; Online: Organic-Inorganic Hybrid Crystal-Assisted Etching of Nickel Foam for the Collectively Exhaustive Electrochemical Performance of Oxygen Evolution Reaction.

Thangasamy, Pitchai / He, Rong / Chen, Xinqi / Yu, Kunpeng / Randriamahazaka, Hyacinthe / Chen, Zheng / Luo, Hongmei / Zhou, Xiao-Dong / Zhou, Meng

Chemistry (Weinheim an der Bergstrasse, Germany)

2023 Volume 29, Issue 52, Page(s) e202301469

Abstract: In this work, an organic-inorganic hybrid crystal, violet-crystal (VC), was used to etch the nickel foam (NF) to fabricate a self-standing electrode for the water oxidation reaction. The efficacy of VC-assisted etching manifests the promising ... ...

Abstract	In this work, an organic-inorganic hybrid crystal, violet-crystal (VC), was used to etch the nickel foam (NF) to fabricate a self-standing electrode for the water oxidation reaction. The efficacy of VC-assisted etching manifests the promising electrochemical performance towards the oxygen evolution reaction (OER), requiring only ~356 and ~376 mV overpotentials to reach 50 and 100 mA cm
Language	English
Publishing date	2023-08-17
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1478547-X
ISSN	1521-3765 ; 0947-6539
ISSN (online)	1521-3765
ISSN	0947-6539
DOI	10.1002/chem.202301469
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